FastQCFastQC Report
Fri 27 May 2016
SRR2138905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451672
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66650.048.4438821
GTATCAA122250.037.3051221
ATCAACG146650.030.7478033
TCAACGC148550.030.354534
CAACGCA150600.029.8844665
TATCAAC155300.029.2557832
AACGCAG157600.028.6093546
ACGCAGA183450.024.554667
CGCAGAG184650.024.3487078
TGGTATC32550.023.1541822
GTGGTAT34500.023.0866871
GCAGAGT220100.020.4270299
TACATGG157850.018.8270662
GAGTACT129750.018.59612512-13
GTACATG162150.018.4598431
AGAGTAC201600.018.0005210-11
CAGAGTA215450.017.92651710-11
ACATGGG162100.017.8579443
GGACCGA8850.017.4178756
ACCGAAC6350.016.8578518
Produced by FastQC (version 0.11.5)