FastQCFastQC Report
Fri 27 May 2016
SRR2138904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7476436
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122200.052.957381
GTATCAA237350.039.040381
ATCAACG277150.032.8922353
TCAACGC281600.032.4462974
TATCAAC283100.032.3640372
CAACGCA287950.031.7018935
AACGCAG296850.030.904296
ACGCAGA338250.027.0601167
CGCAGAG342900.026.681038
TGGTATC49350.025.3669012
GTGGTAT54050.024.315291
GCAGAGT396250.023.0992669
TACATGG331050.022.6134572
GTACATG340650.022.318031
ACATGGG334700.022.0551683
GAGTACT221450.020.83526412-13
CAGAGTA390900.019.89405610-11
CATGGGG209550.019.8264774
GTACTTT253550.018.11558714-15
AGAGTAC376600.017.97717910-11
Produced by FastQC (version 0.11.5)