##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138904_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7476436
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.187390623018775	30.0	18.0	32.0	18.0	33.0
2	26.57660802553516	28.0	18.0	32.0	18.0	33.0
3	29.736490622002247	32.0	27.0	33.0	25.0	33.0
4	30.86786926284128	33.0	31.0	33.0	27.0	33.0
5	32.39819775090698	33.0	33.0	33.0	32.0	34.0
6	36.60279857943009	38.0	37.0	38.0	34.0	38.0
7	36.778393341426316	38.0	37.0	38.0	34.0	38.0
8	37.07067926482618	38.0	38.0	38.0	36.0	38.0
9	37.176665325564215	38.0	38.0	38.0	36.0	38.0
10-11	37.187822312663414	38.0	38.0	38.0	36.0	38.0
12-13	37.24436322333261	38.0	38.0	38.0	36.0	38.0
14-15	37.23535171036039	38.0	38.0	38.0	36.0	38.0
16-17	37.218063860909126	38.0	38.0	38.0	36.0	38.0
18-19	37.23863268541321	38.0	38.0	38.0	36.0	38.0
20-21	37.256583657048665	38.0	38.0	38.0	36.0	38.0
22-23	37.26743506007756	38.0	38.0	38.0	37.0	38.0
24-25	37.26452188765235	38.0	38.0	38.0	37.0	38.0
26-27	37.222000543475346	38.0	38.0	38.0	36.0	38.0
28-29	37.19620082355678	38.0	38.0	38.0	36.0	38.0
30-31	37.2015604752977	38.0	38.0	38.0	36.0	38.0
32-33	37.190105764934934	38.0	38.0	38.0	36.0	38.0
34-35	37.165846694128206	38.0	38.0	38.0	36.0	38.0
36-37	37.14752528658436	38.0	38.0	38.0	36.0	38.0
38-39	37.13091849419039	38.0	38.0	38.0	36.0	38.0
40-41	37.10659808384418	38.0	38.0	38.0	36.0	38.0
42-43	37.072387301565854	38.0	38.0	38.0	36.0	38.0
44-45	37.01955928443324	38.0	38.0	38.0	36.0	38.0
46-47	36.98496439034666	38.0	38.0	38.0	36.0	38.0
48-49	36.94565375401034	38.0	38.0	38.0	36.0	38.0
50-51	36.90876298436474	38.0	38.0	38.0	36.0	38.0
52-53	36.88634056715262	38.0	38.0	38.0	36.0	38.0
54-55	36.862104942531104	38.0	38.0	38.0	35.5	38.0
56-57	36.86328871498476	38.0	38.0	38.0	35.5	38.0
58-59	36.852535081800625	38.0	38.0	38.0	35.0	38.0
60-61	36.8688239407782	38.0	38.0	38.0	35.0	38.0
62-63	36.86542913390633	38.0	38.0	38.0	35.0	38.0
64-65	36.863309346843295	38.0	38.0	38.0	35.0	38.0
66-67	36.85627893445497	38.0	38.0	38.0	35.0	38.0
68-69	36.8687726058497	38.0	38.0	38.0	35.0	38.0
70-71	36.853662392853515	38.0	38.0	38.0	35.0	38.0
72-73	36.84460505829414	38.0	38.0	38.0	35.0	38.0
74-75	36.86575272750887	38.0	38.0	38.0	35.0	38.0
76-77	36.84940604970073	38.0	38.0	38.0	35.0	38.0
78-79	36.82792509956265	38.0	38.0	38.0	35.0	38.0
80-81	36.822242348724714	38.0	38.0	38.0	35.0	38.0
82-83	36.76839927794357	38.0	38.0	38.0	35.0	38.0
84-85	36.773229025602646	38.0	38.0	38.0	35.0	38.0
86-87	36.76724851602679	38.0	38.0	38.0	35.0	38.0
88-89	36.74885414399337	38.0	38.0	38.0	35.0	38.0
90-91	36.73133866456544	38.0	38.0	38.0	35.0	38.0
92-93	36.73561804939249	38.0	38.0	38.0	35.0	38.0
94-95	36.68140297007615	38.0	38.0	38.0	34.5	38.0
96-97	36.644339755905534	38.0	38.0	38.0	34.0	38.0
98-99	36.66921059128951	38.0	38.0	38.0	34.0	38.0
100	35.07249936825228	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	15.0
19	43.0
20	91.0
21	234.0
22	669.0
23	1985.0
24	4832.0
25	9715.0
26	16896.0
27	27438.0
28	40979.0
29	59668.0
30	80792.0
31	104919.0
32	135388.0
33	180872.0
34	266964.0
35	494219.0
36	1528937.0
37	4521776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26891743606178	18.14615948026573	13.451128318359176	22.13379476531331
2	14.102421941868526	20.553180088630693	42.58591039206997	22.758487577430806
3	18.314113837127742	27.13338280432013	30.84095149079053	23.71155186776159
4	11.72517226122179	18.420916062145118	41.25564640692437	28.598265269708723
5	12.399063917736475	38.61733919044614	35.717669756533546	13.265927135283844
6	27.633505880011466	39.18099424712139	19.95331729805304	13.232182574814106
7	25.058263054749613	34.14489203144386	24.173603572611334	16.62324134119519
8	23.42462371108373	37.06370254490241	22.209004397282342	17.302669346731516
9	25.88762881137483	17.042478528539533	22.219798310317913	34.85009434976772
10-11	23.309903274768885	27.49583491385468	29.58826237528148	19.605999436094955
12-13	24.45310171851936	25.45697575689807	29.68895874986424	20.400963774718328
14-15	21.72109143982507	26.411702046269102	27.928487584191185	23.93871892971464
16-17	20.572944114013684	29.6128663443384	28.545559675759947	21.268629865887974
18-19	20.540977010971538	28.554260345437317	30.72924719746146	20.17551544612968
20-21	21.799020707721414	27.58161354169349	30.511144022441528	20.10822172814357
22-23	21.449442466823058	27.523756815548396	30.3105164685051	20.716284249123447
24-25	20.950244346042663	27.66128434056333	30.587811706497405	20.800659606896605
26-27	20.860590722335136	27.86813513501581	30.456681204981408	20.81459293766765
28-29	20.766687422266887	27.985379883700823	30.50830964642844	20.739623047603853
30-31	21.064332947288342	27.784651893761435	30.42209439828679	20.72892076066343
32-33	20.883470776514777	27.87717543713006	30.349365399795573	20.889988386559587
34-35	20.994031383061895	27.946995763326427	30.366915633806247	20.69205721980543
36-37	20.968675575997395	27.844965501687913	30.33972990101286	20.846629021301833
38-39	20.80309215017065	27.958648464163822	30.424395904436864	20.81386348122867
40-41	21.06775821229401	27.870774496158628	30.273483707120825	20.787983584426538
42-43	21.025060407528688	27.874635443695496	30.121678245108818	20.978625903667
44-45	21.076725707571498	28.085773003080984	29.880208403406566	20.957292885940955
46-47	21.079115214992143	28.047476102770773	29.677248851240577	21.196159830996507
48-49	21.134425735017086	28.150857637015193	29.575724723492275	21.13899190447545
50-51	21.10843402478099	28.318517253415216	29.359387837928608	21.213660883875182
52-53	21.195542942090484	28.30532337799937	29.251130832881106	21.24800284702904
54-55	21.266719375110206	28.26671634645536	29.23021270833914	21.236351570095298
56-57	21.241663596900004	28.28307533987111	29.19917031612615	21.276090747102735
58-59	21.172421880331825	28.30286144992849	29.292010872823482	21.2327057969162
60-61	21.236801925691733	28.279777598829163	29.280835991258996	21.202584484220104
62-63	21.159070059193393	28.240916301243242	29.28319470939061	21.316818930172758
64-65	21.138262171824863	28.23132250901745	29.315036759167356	21.31537855999033
66-67	21.22722222423569	28.32774438309319	29.084840847675746	21.360192544995368
68-69	21.2630006861404	28.272628579561786	29.155441300708507	21.308929433589306
70-71	21.228695308911462	28.109574709405344	29.155101945262697	21.506628036420498
72-73	21.265442739323184	28.094645819736357	29.21566446791634	21.424246973024115
74-75	21.218787207302555	28.184473806059003	29.084047162323056	21.512691824315386
76-77	21.248751116651103	28.16643922162409	28.997510518740405	21.5872991429844
78-79	21.31586392446207	28.23522152728584	28.918975865270376	21.529938682981708
80-81	21.31027985899898	28.22557518634829	28.888105855168984	21.576039099483747
82-83	21.418034195706436	28.181083738494905	28.972096910334212	21.428785155464443
84-85	21.521878318755363	28.23767170326948	28.772125366056763	21.468324611918398
86-87	21.30690700548648	28.27759508344346	28.849560916604705	21.56593699446535
88-89	21.417063203127654	28.255934308833513	28.777610831970975	21.549391656067858
90-91	21.461906876093053	28.208059167948434	28.827898593056783	21.50213536290173
92-93	21.418972018382977	28.228649190165246	28.84529581514297	21.50708297630881
94-95	21.270094575013747	28.358687233287363	28.82759442280175	21.543623768897145
96-97	21.413678451481562	28.43580528768248	28.90425948198669	21.246256778849272
98-99	22.38486258991983	30.38700226339609	29.135366057791977	18.092769088892098
100	28.108823264581755	41.31839213670149	30.57278459871675	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2707.0
1	2418.5
2	3056.0
3	4255.0
4	5318.5
5	7468.5
6	9372.5
7	11184.0
8	14074.0
9	15560.0
10	14670.5
11	14105.0
12	15419.5
13	18767.5
14	24975.5
15	31925.5
16	37516.0
17	41947.5
18	42464.5
19	39032.0
20	34939.0
21	32213.5
22	32902.0
23	38158.0
24	47861.5
25	61479.0
26	79983.5
27	100256.5
28	119945.0
29	141393.0
30	161904.5
31	180224.0
32	199312.5
33	219047.0
34	238184.5
35	255541.0
36	272803.5
37	288204.5
38	298837.0
39	306985.0
40	314835.5
41	319325.0
42	320857.0
43	323005.5
44	324778.0
45	323992.0
46	319780.5
47	314450.5
48	306950.0
49	295984.0
50	284094.5
51	268780.5
52	249785.5
53	231042.0
54	211940.0
55	191572.0
56	170586.5
57	150106.0
58	129956.5
59	111235.5
60	93094.0
61	74108.5
62	57087.0
63	43567.0
64	32657.5
65	23962.0
66	17083.0
67	12449.5
68	9099.0
69	6384.5
70	4562.5
71	3232.5
72	2221.0
73	1491.5
74	967.5
75	643.0
76	431.5
77	273.0
78	189.5
79	132.0
80	79.0
81	47.0
82	32.0
83	18.5
84	11.0
85	8.0
86	6.0
87	6.5
88	5.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003276962445742865
3	0.0
4	0.0
5	0.002273810676637906
6	6.687678460699723E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	6.719185161727763E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	3.512425707716147E-4
50-51	0.0014129560388074535
52-53	0.004067855041253976
54-55	0.006123417366218473
56-57	0.007065497987041862
58-59	0.008679105058342287
60-61	0.008309695172936192
62-63	0.0061720993983859765
64-65	0.0036063827210181
66-67	0.001276347515707741
68-69	4.863958276487482E-4
70-71	3.0675916370348077E-4
72-73	5.55656472576678E-4
74-75	0.0
76-77	2.6781142660970987E-4
78-79	2.6274052093647844E-4
80-81	7.727300475306253E-5
82-83	1.739637198662219E-5
84-85	1.409306088510588E-4
86-87	1.1596480307659976E-4
88-89	2.7114626813708683E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	73.0
20-21	236.0
22-23	615.0
24-25	840.0
26-27	2376.0
28-29	6270.0
30-31	19089.0
32-33	24267.0
34-35	32821.0
36-37	50240.0
38-39	58865.0
40-41	59202.0
42-43	67038.0
44-45	78979.0
46-47	80807.0
48-49	76545.0
50-51	68299.0
52-53	64317.0
54-55	66908.0
56-57	69994.0
58-59	71948.0
60-61	70405.0
62-63	69484.0
64-65	71961.0
66-67	75184.0
68-69	76709.0
70-71	75924.0
72-73	72161.0
74-75	72055.0
76-77	74639.0
78-79	75510.0
80-81	76172.0
82-83	72232.0
84-85	71220.0
86-87	72445.0
88-89	75038.0
90-91	75704.0
92-93	74597.0
94-95	84270.0
96-97	220023.0
98-99	1578144.0
100-101	3442830.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.81600075806642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56733200934094	78.62693564378998
2	6.745677476489755	11.712654818252123
3	1.5966698783121667	4.158494800977927
4	0.5562240216416244	1.931565803379761
5	0.22683012734834315	0.9846242253913032
6	0.10655992457323427	0.5550663895517645
7	0.06934844754062475	0.42143884119803254
8	0.03324079478308522	0.23086662920696455
9	0.022548385259849424	0.17618045686310352
>10	0.07375535183671533	0.9687634230498087
>50	0.0012678210295430136	0.07365523012445611
>100	4.55317746560288E-4	0.07491293507835492
>500	4.5222051057214976E-5	0.031663170796122214
>1k	4.5222046459004566E-5	0.05317763234037686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3375356921399447E-5	0.0
2	0.0	0.0	0.0	1.3375356921399447E-5	0.0
3	0.0	0.0	0.0	1.3375356921399447E-5	0.0
4	0.0	0.0	0.0	1.3375356921399447E-5	0.0
5	0.0	4.012607076419834E-5	0.0	1.3375356921399447E-5	0.0
6	0.0	4.012607076419834E-5	0.0	2.6750713842798894E-5	0.0
7	0.0	4.012607076419834E-5	0.0	4.012607076419834E-5	1.3375356921399447E-5
8	0.0	4.012607076419834E-5	0.0	6.687678460699724E-5	1.3375356921399447E-5
9	0.0	6.687678460699724E-5	0.0	8.025214152839669E-5	1.3375356921399447E-5
10-11	0.0	8.025214152839669E-5	0.0	8.693981998909641E-5	1.3375356921399447E-5
12-13	0.0	9.362749844979613E-5	0.0	9.362749844979613E-5	1.3375356921399447E-5
14-15	0.0	1.471289261353939E-4	0.0	1.0031517691049585E-4	1.3375356921399447E-5
16-17	0.0	1.6050428305679337E-4	0.0	1.0700285537119558E-4	1.3375356921399447E-5
18-19	0.0	1.6050428305679337E-4	0.0	1.2706589075329476E-4	6.018910614629751E-5
20-21	0.0	2.0063035382099172E-4	0.0	1.3375356921399448E-4	1.0700285537119558E-4
22-23	0.0	2.273810676637906E-4	0.0	1.3375356921399448E-4	1.0700285537119558E-4
24-25	0.0	2.4075642458519007E-4	0.0	1.8056731843889255E-4	1.0700285537119558E-4
26-27	0.0	2.4075642458519007E-4	0.0	2.1400571074239118E-4	1.0700285537119558E-4
28-29	0.0	2.4075642458519007E-4	0.0	5.216389199345784E-4	1.0700285537119558E-4
30-31	0.0	2.875701738100881E-4	0.0	0.0017655471136247272	1.0700285537119558E-4
32-33	0.0	3.076332091921873E-4	0.0	0.00435367867791552	1.0700285537119558E-4
34-35	0.0	3.410716014956859E-4	0.0	0.007831271477479376	1.0700285537119558E-4
36-37	0.0	4.012607076419834E-4	0.0	0.013549236561377642	1.0700285537119558E-4
38-39	0.0	4.213237430240826E-4	0.0	0.028061498821096043	1.0700285537119558E-4
40-41	0.0	4.413867784061818E-4	0.0	0.04472719354515975	1.0700285537119558E-4
42-43	0.0	4.413867784061818E-4	0.0	0.05521347337153692	1.136905338318953E-4
44-45	0.0	4.547621353275812E-4	0.0	0.06747198798999951	1.2037821229259503E-4
46-47	0.0	4.6813749224898065E-4	0.0	0.08086072026832036	1.2037821229259503E-4
48-49	0.0	4.6813749224898065E-4	0.0	0.0943163293312482	1.2037821229259503E-4
50-51	0.0	5.082635630131791E-4	0.0	0.10796588106953633	1.3375356921399448E-4
52-53	0.0	5.350142768559779E-4	0.0	0.14320994655742386	1.3375356921399448E-4
54-55	0.0	5.617649906987768E-4	0.0	0.18605790245512702	1.3375356921399448E-4
56-57	0.0	5.751403476201763E-4	0.0	0.23644287197803873	1.3375356921399448E-4
58-59	0.0	5.885157045415756E-4	0.0	0.27608742989306667	1.3375356921399448E-4
60-61	0.0	5.885157045415756E-4	0.0	0.2906331305450886	1.5381660459609365E-4
62-63	0.0	6.152664183843746E-4	0.0	0.3081481604336612	1.6719196151749309E-4
64-65	0.0	6.28641775305774E-4	0.0	0.3165946983295249	1.7387963997819283E-4
66-67	0.0	6.487048106878732E-4	0.0	0.32336262893175305	1.7387963997819283E-4
68-69	0.0	6.687678460699723E-4	0.0	0.330645510775455	1.8725499689959226E-4
70-71	0.0	6.754555245306721E-4	0.0	0.33733318923615474	2.0731803228169144E-4
72-73	0.0	6.821432029913718E-4	0.0	0.34306452967697443	2.1400571074239115E-4
74-75	0.0	6.821432029913718E-4	0.0	0.34735801924874365	2.273810676637906E-4
76-77	0.0	7.222692737555702E-4	0.0	0.351404064717467	2.273810676637906E-4
78-79	0.0	7.49019987598369E-4	0.0	0.3539453825325329	2.273810676637906E-4
80-81	0.0	7.623953445197685E-4	0.0	0.35478803001858106	2.3406874612449035E-4
82-83	0.0	7.623953445197685E-4	0.0	0.35531635661697636	2.474441030458898E-4
84-85	0.0	7.757707014411679E-4	0.0	0.3555838637554043	2.5413178150658953E-4
86-87	0.0	7.824583799018676E-4	0.0	0.3557176173246183	2.5413178150658953E-4
88	0.0	8.292721291267657E-4	0.0	0.3557443680384611	2.5413178150658953E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12220	0.0	52.95738	1
GTATCAA	23735	0.0	39.04038	1
ATCAACG	27715	0.0	32.892235	3
TCAACGC	28160	0.0	32.446297	4
TATCAAC	28310	0.0	32.364037	2
CAACGCA	28795	0.0	31.701893	5
AACGCAG	29685	0.0	30.90429	6
ACGCAGA	33825	0.0	27.060116	7
CGCAGAG	34290	0.0	26.68103	8
TGGTATC	4935	0.0	25.366901	2
GTGGTAT	5405	0.0	24.31529	1
GCAGAGT	39625	0.0	23.099266	9
TACATGG	33105	0.0	22.613457	2
GTACATG	34065	0.0	22.31803	1
ACATGGG	33470	0.0	22.055168	3
GAGTACT	22145	0.0	20.835264	12-13
CAGAGTA	39090	0.0	19.894056	10-11
CATGGGG	20955	0.0	19.826477	4
GTACTTT	25355	0.0	18.115587	14-15
AGAGTAC	37660	0.0	17.977179	10-11
>>END_MODULE