FastQCFastQC Report
Fri 27 May 2016
SRR2138903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6690934
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80450.050.32851
GTATCAA155350.038.66921
TCAACGC181900.032.27454
ATCAACG185700.031.5910913
CAACGCA186400.031.4495685
AACGCAG191250.030.7842626
TATCAAC197700.029.9972842
ACGCAGA220850.026.5617337
CGCAGAG223650.026.2101148
GCAGAGT264700.022.1776529
AGAGTAC236700.019.89857110-11
GAGTACT151150.019.88492612-13
GTACATG186200.019.3391151
TACATGG182850.019.2967572
CAGAGTA258700.018.8825310-11
ACATGGG186450.018.6038133
AGTACTT169750.017.1657212-13
GTACTTT175550.017.03672614-15
CATGGGG120900.016.6214314
GTATAAT49650.014.3506321
Produced by FastQC (version 0.11.5)