FastQCFastQC Report
Fri 27 May 2016
SRR2138902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81243089
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1119900.054.021851
GTATCAA2153900.039.3083341
TCAACGC2706300.030.63284
ATCAACG2743150.030.218183
CAACGCA2763100.030.0069835
TATCAAC2812300.029.662042
AACGCAG2841900.029.2135316
ACGCAGA3282250.025.272117
CGCAGAG3333450.024.8647278
GCAGAGT3881200.021.2655079
GTACATG2622150.020.8089181
TACATGG2627500.020.2933242
AGAGTAC3448450.019.8286210-11
GAGTACT2259250.019.65507112-13
ACATGGG2660850.019.5620483
CAGAGTA3747800.018.79303610-11
CTTATAC795950.017.7849141
AGTACTT2478650.017.74123212-13
CATGGGG1936850.017.6640364
GTACTTT2569950.017.2756214-15
Produced by FastQC (version 0.11.5)