##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81243089
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2311576803782	33.0	31.0	34.0	30.0	34.0
2	32.26563907485103	34.0	31.0	34.0	30.0	34.0
3	32.367278120111855	34.0	31.0	34.0	30.0	34.0
4	35.964162748661614	37.0	35.0	37.0	35.0	37.0
5	35.899287679718825	37.0	35.0	37.0	35.0	37.0
6	35.971612686957286	37.0	35.0	37.0	35.0	37.0
7	35.90604952256308	37.0	35.0	37.0	35.0	37.0
8	35.91011049567552	37.0	35.0	37.0	35.0	37.0
9	37.5983929660774	39.0	37.0	39.0	35.0	39.0
10-11	37.54826430221037	39.0	37.0	39.0	35.0	39.0
12-13	37.51592925645651	39.0	37.0	39.0	35.0	39.0
14-15	38.84635669749091	40.0	38.0	41.0	35.0	41.0
16-17	38.8522255659186	40.0	38.0	41.0	35.0	41.0
18-19	38.90823162816963	40.0	38.0	41.0	35.0	41.0
20-21	38.92477564985366	40.0	38.0	41.0	35.0	41.0
22-23	38.89249507883429	40.0	38.0	41.0	35.0	41.0
24-25	38.86617665402158	40.0	38.0	41.0	34.0	41.0
26-27	38.77360456508303	40.0	38.0	41.0	34.0	41.0
28-29	38.68821779734326	40.0	38.0	41.0	34.0	41.0
30-31	38.58908189422368	40.0	38.0	41.0	34.0	41.0
32-33	38.50139867072331	40.0	38.0	41.0	34.0	41.0
34-35	38.28750055325336	40.0	38.0	41.0	33.5	41.0
36-37	38.304572544847865	40.0	38.0	41.0	33.5	41.0
38-39	38.33939897200334	40.0	38.0	41.0	34.0	41.0
40-41	38.32048325446252	40.0	38.0	41.0	33.0	41.0
42-43	38.23821042001217	40.0	38.0	41.0	33.0	41.0
44-45	38.21820736376115	40.0	38.0	41.0	33.0	41.0
46-47	38.1521316018101	40.0	37.0	41.0	33.0	41.0
48-49	38.08822577122298	40.0	37.0	41.0	33.0	41.0
50-51	37.994792681612175	40.0	37.0	41.0	33.0	41.0
52-53	37.91476842702005	40.0	37.0	41.0	33.0	41.0
54-55	37.77305777986503	40.0	36.5	41.0	33.0	41.0
56-57	37.61792724741083	39.5	36.0	41.0	32.0	41.0
58-59	37.4480108596192	39.0	36.0	41.0	32.0	41.0
60-61	37.250994087067966	39.0	35.0	41.0	32.0	41.0
62-63	37.025724998884996	39.0	35.0	41.0	31.5	41.0
64-65	36.763582709530596	38.0	35.0	40.0	31.0	41.0
66-67	36.374159596978515	37.5	35.0	40.0	31.0	41.0
68-69	36.13804972904862	37.0	35.0	40.0	31.0	41.0
70-71	35.885008209878315	36.5	35.0	39.0	31.0	41.0
72-73	35.49466338848047	36.0	34.0	39.0	30.0	41.0
74-75	35.25954694736521	35.5	34.0	38.5	30.0	40.0
76-77	34.96761563026675	35.0	34.0	37.0	30.0	39.5
78-79	34.66013994621793	35.0	34.0	37.0	30.0	39.0
80-81	34.23823456063609	35.0	34.0	36.5	29.5	39.0
82-83	33.88378341789222	35.0	33.5	36.0	29.5	37.5
84-85	33.83793221324595	35.0	34.0	36.0	29.5	37.0
86-87	33.890306843483614	35.0	34.0	35.0	30.0	37.0
88-89	33.850511298178326	35.0	34.0	35.0	30.0	36.0
90-91	33.56827717132688	35.0	34.0	35.0	29.5	36.0
92-93	33.515193531200545	35.0	34.0	35.0	30.0	36.0
94-95	33.51008943163827	35.0	34.0	35.0	30.0	36.0
96-97	33.52683108941902	35.0	34.0	35.0	30.0	35.5
98-99	33.647343713717646	35.0	34.0	35.0	30.0	35.0
100	33.3729503485198	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	13.0
19	109.0
20	505.0
21	2827.0
22	12588.0
23	43663.0
24	112118.0
25	235526.0
26	415992.0
27	651162.0
28	922316.0
29	1217002.0
30	1542965.0
31	1915389.0
32	2380944.0
33	3008185.0
34	3980152.0
35	5546244.0
36	8655993.0
37	1.627342E7
38	2.2013461E7
39	1.160584E7
40	706672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35324544343704	20.20151892550516	13.360992465463738	25.08424316559406
2	14.69116363474515	22.20815141837051	39.622915367274075	23.477769579610268
3	17.193750473963394	27.577602323811195	31.136713671731513	24.091933530493897
4	12.425907390104284	18.93700767581597	38.909339106985456	29.72774582709429
5	12.739798169191749	38.50423685081078	35.570780816589846	13.185184163407628
6	28.577506451041025	39.20681917941353	18.916727796994522	13.298946572550928
7	25.10038607714682	33.50199055085166	23.89071764614957	17.506905725851954
8	24.70998363934685	35.868185661921345	21.569065400750578	17.852765297981225
9	25.860972617621663	15.939973675791672	20.85349068891263	37.34556301767403
10-11	23.74250697938775	26.584708943756652	29.43922874186481	20.233555334990793
12-13	25.118733739497628	24.41716430341447	29.18149619066958	21.28260576641832
14-15	22.476291456274677	25.35341198496002	26.74473681293601	25.425559745829297
16-17	21.80869701611591	28.49707834514838	27.19132115336412	22.502903485371586
18-19	21.955770908710768	27.51014981717399	29.022713550441193	21.511365723674047
20-21	22.757683902087706	26.6278909873374	28.84807007289006	21.766355037684836
22-23	22.486251074037934	26.578381698940834	28.78945339053788	22.14591383648336
24-25	22.249118374921586	26.874368344741534	28.77823173976384	22.09828154057304
26-27	22.207254777956795	26.889174522624398	28.78212081410189	22.121449885316917
28-29	22.149012982660622	26.700307659784194	28.84389734355276	22.306782014002422
30-31	22.419998683629323	26.612183615680024	28.738626081946173	22.229191618744476
32-33	22.241533828807533	26.883998874547327	28.69714128760541	22.177326009039728
34-35	22.49691939557675	26.947060867342227	28.65342422904008	21.902595508040946
36-37	22.472260734215705	26.80922347649784	28.50693421021331	22.211581579073144
38-39	22.365469170338017	26.875695505482515	28.564667515304553	22.19416780887492
40-41	22.50694106734621	26.705563829857148	28.529855333358196	22.257639769438445
42-43	22.502734308245202	26.810256527885702	28.2957812796721	22.391227884197
44-45	22.41798353195988	26.976091262629392	28.12286940209438	22.483055803316343
46-47	22.729766885670006	26.919109461308544	27.681991741062827	22.669131911958626
48-49	22.59220047764013	27.094083374510607	27.588625858647315	22.725090289201948
50-51	22.557840979146484	27.243007050388933	27.49369121981957	22.705460750645006
52-53	22.71830195555509	27.150781116794743	27.456015487238965	22.674901440411197
54-55	22.66108021478603	27.063395222134684	27.475061842439573	22.800462720639715
56-57	22.699846364638997	27.186388830810422	27.40606169440616	22.70770311014443
58-59	22.571079462478533	27.288512255329106	27.485814572236773	22.654593709955588
60-61	22.813818468267748	27.33985909651541	27.313973358174138	22.532349077042703
62-63	22.631049429866035	27.540272414869253	27.238783558722286	22.589894596542422
64-65	22.636275749870347	27.41627567282734	27.203042205674084	22.744406371628234
66-67	22.76744195603864	27.481266171195003	27.11518953259607	22.636102340170297
68-69	22.635936778768972	27.324757291314977	27.21035097827783	22.828954951638224
70-71	22.730887366065474	27.03423096862275	27.30258685986204	22.932294805449736
72-73	22.83276365030769	27.100164179410758	27.133868877827044	22.933203292454508
74-75	22.5754665250394	27.226244629240874	27.241860211837515	22.956428633882208
76-77	22.75763179015502	27.1151590077202	27.169356629259507	22.95785257286527
78-79	22.698547164120146	27.182180893660025	27.110008147252884	23.009263794966945
80-81	22.877587333391183	27.231996853272733	26.991915939473206	22.898499873862878
82-83	22.895068013227633	27.163451927660848	27.08921453981421	22.85226551929731
84-85	22.770521341242365	27.173926159104372	27.162853395129005	22.892699104524258
86-87	22.670740689193167	27.20190883583068	27.214175997505713	22.913174477470438
88-89	22.68366470927709	27.172503446575334	27.162716848253588	22.981114995893986
90-91	22.901765125161877	27.147295733371568	27.184478054058204	22.76646108740835
92-93	22.839338463970773	27.13072353260646	27.16209174746166	22.867846255961112
94-95	22.633588229757084	27.17829294226971	27.249386999532817	22.93873182844039
96-97	22.721359281782405	27.31500129204762	27.293795391881908	22.66984403428807
98-99	23.786858831613785	29.24437605519078	27.411608923043175	19.557156190152263
100	22.798184953623295	30.163115367861437	21.23275375438181	25.80594592413346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27793.0
1	25830.5
2	36428.0
3	49747.5
4	60187.5
5	97073.0
6	132366.0
7	161053.0
8	182440.5
9	174274.0
10	138080.0
11	100635.0
12	100164.5
13	135707.5
14	230301.5
15	363803.0
16	467590.0
17	516917.5
18	514100.5
19	470098.0
20	405844.0
21	346443.5
22	315991.0
23	331550.5
24	391258.5
25	491446.5
26	623031.5
27	761994.5
28	897628.0
29	1052706.5
30	1209141.5
31	1363333.0
32	1540894.0
33	1714035.5
34	1879326.5
35	2031785.5
36	2204247.0
37	2375333.5
38	2506976.0
39	2618136.5
40	2731190.0
41	2838284.5
42	2938278.5
43	3059114.0
44	3203845.0
45	3350803.0
46	3405817.0
47	3438217.5
48	3457759.0
49	3408647.0
50	3359596.5
51	3293101.5
52	3189857.0
53	3052566.0
54	2901205.5
55	2733030.5
56	2539119.5
57	2320410.0
58	2083020.5
59	1819313.5
60	1516651.0
61	1229056.5
62	979891.5
63	755897.5
64	562086.0
65	411353.0
66	300157.0
67	224873.0
68	170142.5
69	123439.0
70	88289.0
71	62605.5
72	42883.5
73	28349.5
74	18599.0
75	12178.5
76	7856.5
77	4956.5
78	3218.5
79	2181.0
80	1551.0
81	1090.0
82	810.0
83	589.0
84	426.0
85	316.0
86	235.0
87	169.0
88	104.5
89	68.0
90	55.0
91	39.5
92	26.5
93	14.5
94	8.0
95	11.0
96	15.5
97	20.0
98	28.0
99	54.0
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.23087392701181E-6
3	0.0
4	0.0
5	0.05951152349709401
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.003883407239722261
12-13	7.336008604990389E-4
14-15	0.002677766228214193
16-17	0.009801449080795045
18-19	0.0
20-21	7.878043649851832E-5
22-23	9.972358530444436E-5
24-25	1.354607835243827E-5
26-27	2.2174145116795596E-4
28-29	0.03303555722191001
30-31	0.04737121537490717
32-33	0.03703838686269464
34-35	0.006662334061474389
36-37	0.010074122207674379
38-39	8.039873467783126E-4
40-41	1.0701516579271053E-5
42-43	3.801830449564676E-6
44-45	6.099989209087152E-4
46-47	4.3917631778659055E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.004899347020182807
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.5913369425503455E-6
74-75	7.245201356426312E-7
76-77	1.8272717709783515E-5
78-79	0.01010750164325296
80-81	0.0
82-83	0.0
84-85	0.0
86-87	8.530061920872195E-5
88-89	1.0254243957739184E-4
90-91	0.0
92-93	1.0360962215228555E-5
94-95	0.0
96-97	0.003248886190155494
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2708.0
20-21	11164.0
22-23	19988.0
24-25	24914.0
26-27	42742.0
28-29	84951.0
30-31	160667.0
32-33	212492.0
34-35	278506.0
36-37	378544.0
38-39	473820.0
40-41	506626.0
42-43	590483.0
44-45	736641.0
46-47	719583.0
48-49	640132.0
50-51	544958.0
52-53	484831.0
54-55	512704.0
56-57	550099.0
58-59	609045.0
60-61	618472.0
62-63	588211.0
64-65	624247.0
66-67	666750.0
68-69	702888.0
70-71	691912.0
72-73	606532.0
74-75	596502.0
76-77	628879.0
78-79	675492.0
80-81	703283.0
82-83	672794.0
84-85	651736.0
86-87	681161.0
88-89	767080.0
90-91	829130.0
92-93	872511.0
94-95	1036469.0
96-97	2783607.0
98-99	5128851.0
100-101	5.3130984E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.81144545159154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.82055846037014	61.030960200591785
2	10.127007419942663	14.74724096690036
3	2.7675889122310826	6.045364473460291
4	1.2114835511561939	3.5283947400203846
5	0.633124551834947	2.304935688499679
6	0.38065821758491647	1.662976502723052
7	0.258371834567383	1.3168698717181448
8	0.17274719609155584	1.0062378436108566
9	0.12222771159167549	0.8009618719707129
>10	0.48692313130971226	5.772413730357
>50	0.01308881564520403	0.6511726234434366
>100	0.005752986123081435	0.8044165214162199
>500	3.7102409903725953E-4	0.18286814547652558
>1k	8.933548336418507E-5	0.09649899667544554
>5k	6.851968955903299E-6	0.04868782313595148
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2308739270118103E-6
2	0.0	3.3233596029318875E-5	0.0	0.0	2.4617478540236206E-6
3	2.4617478540236206E-6	3.446446995633069E-5	0.0	0.0	2.4617478540236206E-6
4	2.4617478540236206E-6	7.754505740174405E-5	0.0	1.2308739270118103E-6	2.4617478540236206E-6
5	6.154369635059051E-6	8.862292274485033E-5	0.0	1.2308739270118103E-6	2.4617478540236206E-6
6	2.7079226394259824E-5	8.862292274485033E-5	0.0	1.2308739270118103E-6	3.6926217810354307E-6
7	2.7079226394259824E-5	8.985379667186214E-5	0.0	1.2308739270118103E-6	3.6926217810354307E-6
8	2.8310100321271633E-5	1.0093166201496844E-4	0.0	1.2308739270118103E-6	6.154369635059051E-6
9	2.8310100321271633E-5	1.673988540736062E-4	0.0	2.4617478540236206E-6	9.846991416094482E-6
10-11	3.015641121178935E-5	1.698606019276298E-4	0.0	2.4617478540236206E-6	9.846991416094482E-6
12-13	3.3233596029318875E-5	1.7909215638021837E-4	0.0	4.923495708047241E-6	1.1693302306612197E-5
14-15	4.246515048190745E-5	2.4740565932937383E-4	0.0	4.923495708047241E-6	2.707922639425982E-5
16-17	6.09282593870846E-5	2.5786808770897427E-4	0.0	6.769806598564957E-6	3.815709173736612E-5
18-19	6.831350294915546E-5	2.7756207054116316E-4	0.0	1.4155050160635817E-5	4.861952011696651E-5
20-21	7.077525080317909E-5	3.095647926434703E-4	0.0	2.2771167649718492E-5	5.846651153306098E-5
22-23	7.692962043823813E-5	3.520299431253777E-4	0.0	3.938796566437792E-5	7.077525080317909E-5
24-25	8.185311614628538E-5	3.9326421968027337E-4	0.0	7.446787258421451E-5	7.754505740174405E-5
26-27	8.36994270368031E-5	4.228051939285568E-4	0.0	1.5139749302245264E-4	7.754505740174405E-5
28-29	8.49303009638149E-5	4.2772868963660406E-4	0.0	5.680483173159505E-4	8.000680525576767E-5
30-31	8.739204881783852E-5	4.837334533156414E-4	0.0	0.0018173853532329378	8.36994270368031E-5
32-33	9.046923363536805E-5	4.948113186587478E-4	0.0	0.004202819023781826	8.862292274485033E-5
34-35	9.78544771974389E-5	5.569704519728441E-4	0.0	0.007548334357399926	1.0462428379600386E-4
36-37	1.0154709897847435E-4	6.246685179584937E-4	0.0	0.013631313304692291	1.0708603165002749E-4
38-39	1.0339340986899206E-4	6.671336684404012E-4	0.0	0.03399673786406619	1.1016321646755702E-4
40-41	1.0954777950405111E-4	7.477559106596747E-4	0.0	0.05717224759880807	1.1385583824859245E-4
42-43	1.1077865343106292E-4	7.779123218714641E-4	0.0	0.06959053464843021	1.1693302306612197E-4
44-45	1.1631758610261607E-4	8.474566987476313E-4	0.0	0.08472536045496744	1.193947709201456E-4
46-47	1.218565187741692E-4	8.936144710105741E-4	0.0	0.10027929883365218	1.2985719929974596E-4
48-49	1.2247195573767512E-4	8.991534036821274E-4	0.0	0.11513963975446576	1.4278137553336997E-4
50-51	1.3354982108078141E-4	0.001017932737638767	0.0	0.12994459134856381	1.446276864238877E-4
52-53	1.4278137553337E-4	0.001030241476908885	0.0	0.17365415537068019	1.6062904747504123E-4
54-55	1.446276864238877E-4	0.0010560898293761332	0.0	0.22187979583100292	1.7663040852619475E-4
56-57	1.452431233873936E-4	0.001077014686135334	0.0	0.2683926752218887	1.803230303072302E-4
58-59	1.4647399731440542E-4	0.0010880925514784403	0.0	0.310057142214275	1.8770827386930107E-4
60-61	1.5447467783998218E-4	0.001102247601639076	0.0	0.3282556132251446	1.957089543948778E-4
62-63	1.673988540736062E-4	0.001115787214836206	0.0	0.350386726432817	2.0063245010292505E-4
64-65	1.673988540736062E-4	0.0012099490702526094	0.0	0.35840279288248134	2.0186332402993688E-4
66-67	1.710914758546416E-4	0.0012290276161212926	0.0	0.3637897126240486	2.1478750026356086E-4
68-69	1.7539953459918295E-4	0.001258568590369576	0.0	0.3692184820791341	2.2094186989861993E-4
70-71	1.81553904234242E-4	0.0012856478167638358	0.0	0.3738188733813408	2.2832711346069078E-4
72-73	2.0247876099344276E-4	0.0013071881104865426	0.0	0.3774154869960693	2.5294459200092697E-4
74-75	2.037096349204546E-4	0.0013533458827494852	0.0	0.3802089553734226	2.535600289644329E-4
76-77	2.0678681973798413E-4	0.0014210439487351348	0.0	0.38290518471054197	2.554063398549506E-4
78-79	2.0924856759200772E-4	0.0014450459903118651	0.0	0.38476934327300133	2.566372137819624E-4
80-81	2.1047944151901955E-4	0.0014512003599469242	0.0	0.3854204755803906	2.683305160885746E-4
82-83	2.1232575240953726E-4	0.001457970166545489	0.0	0.3858937466053266	2.806392553586927E-4
84-85	2.1355662633654909E-4	0.0014690480318885956	0.0	0.38603468166996946	2.8310100321271635E-4
86-87	2.1417206330005497E-4	0.001491819199538314	0.0	0.3860691461399258	2.8433187713972817E-4
88	2.2278818078913764E-4	0.001589058239772247	0.0	0.38607099245081633	2.8679362499375177E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	111990	0.0	54.02185	1
GTATCAA	215390	0.0	39.308334	1
TCAACGC	270630	0.0	30.6328	4
ATCAACG	274315	0.0	30.21818	3
CAACGCA	276310	0.0	30.006983	5
TATCAAC	281230	0.0	29.66204	2
AACGCAG	284190	0.0	29.213531	6
ACGCAGA	328225	0.0	25.27211	7
CGCAGAG	333345	0.0	24.864727	8
GCAGAGT	388120	0.0	21.265507	9
GTACATG	262215	0.0	20.808918	1
TACATGG	262750	0.0	20.293324	2
AGAGTAC	344845	0.0	19.82862	10-11
GAGTACT	225925	0.0	19.655071	12-13
ACATGGG	266085	0.0	19.562048	3
CAGAGTA	374780	0.0	18.793036	10-11
CTTATAC	79595	0.0	17.784914	1
AGTACTT	247865	0.0	17.741232	12-13
CATGGGG	193685	0.0	17.664036	4
GTACTTT	256995	0.0	17.27562	14-15
>>END_MODULE