FastQCFastQC Report
Fri 27 May 2016
SRR2138901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85479774
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1122950.051.4940071
GTATCAA2079400.037.8473131
TCAACGC2537650.030.367294
ATCAACG2550600.030.1996253
CAACGCA2592900.029.7046745
TATCAAC2684100.028.9170342
AACGCAG2680050.028.7889186
ACGCAGA3059200.025.1928087
CGCAGAG3116550.024.7333538
GCAGAGT3673900.020.946059
GTACATG2454900.019.5372241
TACATGG2405650.019.3885062
AGAGTAC3300800.019.32173710-11
GAGTACT2257550.019.18921712-13
ACATGGG2415600.018.7889023
CAGAGTA3576400.018.577910-11
AGTACTT2453450.017.28036912-13
GTACTTT2563000.016.83592414-15
CATGGGG1659500.016.408144
ACTTTTT3000500.014.58094316-17
Produced by FastQC (version 0.11.5)