##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85479774
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24403825634822	33.0	31.0	34.0	30.0	34.0
2	32.28519918641806	34.0	31.0	34.0	30.0	34.0
3	32.385326299529055	34.0	31.0	34.0	30.0	34.0
4	35.97609992511211	37.0	35.0	37.0	35.0	37.0
5	35.91383631875302	37.0	35.0	37.0	35.0	37.0
6	35.984501994588804	37.0	35.0	37.0	35.0	37.0
7	35.924768296649916	37.0	35.0	37.0	35.0	37.0
8	35.927286506396236	37.0	35.0	37.0	35.0	37.0
9	37.61772396590567	39.0	37.0	39.0	35.0	39.0
10-11	37.57375481596383	39.0	37.0	39.0	35.0	39.0
12-13	37.54491079960039	39.0	37.0	39.0	35.0	39.0
14-15	38.88505836480101	40.0	38.0	41.0	35.0	41.0
16-17	38.892086308042884	40.0	38.0	41.0	35.0	41.0
18-19	38.9465521984183	40.0	38.0	41.0	35.0	41.0
20-21	38.96272120991463	40.0	38.0	41.0	35.0	41.0
22-23	38.93128385884461	40.0	38.0	41.0	35.0	41.0
24-25	38.90990264901066	40.0	38.0	41.0	35.0	41.0
26-27	38.824930843672725	40.0	38.0	41.0	34.5	41.0
28-29	38.74371421932902	40.0	38.0	41.0	34.0	41.0
30-31	38.64880695242135	40.0	38.0	41.0	34.0	41.0
32-33	38.56869494826116	40.0	38.0	41.0	34.0	41.0
34-35	38.360465300337	40.0	38.0	41.0	33.5	41.0
36-37	38.384148302590766	40.0	38.0	41.0	34.0	41.0
38-39	38.4283655987077	40.0	38.0	41.0	34.0	41.0
40-41	38.41353236873782	40.0	38.0	41.0	34.0	41.0
42-43	38.337415415844745	40.0	38.0	41.0	33.0	41.0
44-45	38.329437640457726	40.0	38.0	41.0	33.0	41.0
46-47	38.277334291217635	40.0	38.0	41.0	33.0	41.0
48-49	38.22517074913083	40.0	38.0	41.0	33.0	41.0
50-51	38.150539359994696	40.0	37.0	41.0	33.0	41.0
52-53	38.08096133414625	40.0	37.0	41.0	33.0	41.0
54-55	37.96376862424037	40.0	37.0	41.0	33.0	41.0
56-57	37.83160388940297	40.0	36.5	41.0	33.0	41.0
58-59	37.6826404399053	39.5	36.0	41.0	32.5	41.0
60-61	37.513685407938794	39.0	36.0	41.0	32.0	41.0
62-63	37.31353089500193	39.0	35.5	41.0	32.0	41.0
64-65	37.073671547313495	39.0	35.0	41.0	31.5	41.0
66-67	36.707599954129066	38.0	35.0	40.0	31.0	41.0
68-69	36.484032169196745	37.5	35.0	40.0	31.0	41.0
70-71	36.23636021287266	37.0	35.0	39.5	31.0	41.0
72-73	35.844577165015565	36.5	35.0	39.0	31.0	41.0
74-75	35.60299188688674	36.0	34.0	39.0	31.0	40.5
76-77	35.29376906651453	35.5	34.0	38.5	31.0	40.0
78-79	34.961236414675994	35.0	34.0	37.0	30.0	39.0
80-81	34.511920447360794	35.0	34.0	37.0	30.0	39.0
82-83	34.128046921716575	35.0	34.0	36.5	29.5	39.0
84-85	34.05098780891697	35.0	34.0	36.0	29.5	37.0
86-87	34.06800877186818	35.0	34.0	36.0	30.0	37.0
88-89	33.998613232132364	35.0	34.0	35.5	30.0	37.0
90-91	33.69557179919952	35.0	34.0	35.0	30.0	36.0
92-93	33.62374571655171	35.0	34.0	35.0	30.0	36.0
94-95	33.602407885160694	35.0	34.0	35.0	30.0	36.0
96-97	33.60453039629009	35.0	34.0	35.0	30.0	36.0
98-99	33.715438619174975	35.0	34.0	35.0	30.5	35.5
100	33.44274181565214	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	30.0
19	107.0
20	516.0
21	2538.0
22	11420.0
23	39278.0
24	102757.0
25	219471.0
26	395267.0
27	627459.0
28	905343.0
29	1212683.0
30	1547856.0
31	1931278.0
32	2414984.0
33	3062602.0
34	4039455.0
35	5579715.0
36	8579590.0
37	1.6083969E7
38	2.400895E7
39	1.3883687E7
40	830815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.485036846260265	20.844637469443942	13.756230801452517	24.91409488284328
2	14.234001466452204	22.645153990349904	40.257464654912745	22.86337988828515
3	16.89957673495955	27.946684791188147	31.364049933028603	23.789688540823704
4	12.018140104113986	19.58134447103241	39.998704254880224	28.401811169973378
5	12.331705663661396	38.77556830243315	36.207886024033826	12.684840009871627
6	27.621534188894792	40.11717789520595	19.59139597163652	12.669891944262746
7	24.55232275181261	33.75245470349512	24.68391645490312	17.011306089789148
8	23.895556860035686	36.62981373815986	22.129625658579773	17.345003743224684
9	25.444462452603116	16.471419309087082	21.5754980821545	36.5086201561553
10-11	23.106213266295498	27.183192570094146	30.107225836919145	19.603368326691214
12-13	24.507354958094396	24.955874322602284	29.814189990514762	20.722580728788557
14-15	21.808932952463213	26.045099156788513	27.44341509232939	24.70255279841889
16-17	21.131281732980607	29.11182470080004	27.957157108458215	21.799736457761146
18-19	21.218900274584254	28.274133597966696	29.703159954540826	20.803806172908228
20-21	22.08919987628968	27.443639003010787	29.499368699132965	20.967792421566564
22-23	21.74774403559615	27.35866200146034	29.41063649374823	21.482957469195284
24-25	21.542450342566163	27.539491037917614	29.414601902707066	21.503456716809154
26-27	21.56363690833967	27.674374439983236	29.288915000330114	21.473073651346986
28-29	21.55983956414265	27.610550119472432	29.36888969413472	21.4607206222502
30-31	21.83601303175448	27.48903112011115	29.259698704344945	21.41525714378943
32-33	21.649201730402588	27.666720760605052	29.1937362158173	21.490341293175057
34-35	21.859946892150496	27.710310496947834	29.11393080713684	21.315811803764834
36-37	21.80452650346499	27.598538393313767	29.10809142249467	21.488843680726568
38-39	21.65363706232699	27.711505568857113	29.142865633156894	21.491991735659003
40-41	21.857850717599124	27.59966377983535	28.99767095809439	21.544814544471137
42-43	21.848472047288112	27.686008699869973	28.77603355377226	21.689485699069653
44-45	21.8523761951524	27.812187428171004	28.597330355656926	21.73810602101967
46-47	21.97993671853948	27.814622019935364	28.24829697271457	21.957144288810586
48-49	21.958230688040768	27.906422200253957	28.205980401627222	21.929366710078057
50-51	21.95142528856862	28.03585266821998	28.061538463832715	21.951183579378686
52-53	22.113556251655524	27.93422484598812	27.989488433747926	21.962730468608427
54-55	22.072353291142893	27.870231591441865	28.051157221235112	22.006257896180127
56-57	22.055036295519855	27.954051935868097	28.010132641011133	21.98077912760092
58-59	22.040582451092693	27.988159890889904	28.009258193403515	21.96199946461389
60-61	22.134948272416803	27.954105134069827	27.981198273574986	21.929748319938376
62-63	22.06466575078269	28.072076724333016	27.93246359035796	21.93079393452633
64-65	22.05194369782402	27.981260533911616	27.935400200241272	22.03139556802309
66-67	22.216373705310062	28.05810331162369	27.79125962896798	21.934263354098267
68-69	22.06195579585623	27.998470035990735	27.88873343308612	22.050840735066913
70-71	22.122187460483666	27.755345016424076	27.940805221071262	22.181662302020992
72-73	22.186737099994584	27.846349354794064	27.85146547262341	22.11544807258794
74-75	21.968863812716425	27.959036362357843	27.88845803247143	22.1836417924543
76-77	22.106231616258327	27.91426274657505	27.80846603995986	22.17103959720676
78-79	22.08050499100967	27.9519281807458	27.782662497369508	22.184904330875025
80-81	22.195710759536453	27.96550334974927	27.715723046292318	22.123062844421955
82-83	22.23444514344077	27.933319612764212	27.777793282838424	22.054441960956595
84-85	22.163971908381654	27.88644095657974	27.833511502795456	22.116075632243152
86-87	22.01624667660158	27.92850009141443	27.894055893509957	22.161197338474032
88-89	22.083331568573175	27.92636131616335	27.83046594135701	22.159841173906468
90-91	22.290274855778776	27.95698102723778	27.804975370593667	21.947768746389784
92-93	22.214844753981712	27.9131877053931	27.798991062701905	22.07297647792328
94-95	22.02740842963074	27.98136986682509	27.860146418160703	22.131075285383464
96-97	22.07504161256849	28.133507151402576	27.932949139322698	21.858502096706232
98-99	23.108859890204037	30.045639669198653	28.130545239995087	18.714955200602226
100	22.128376252068882	31.060070831159504	21.969685026331014	24.841867890440604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15926.0
1	16037.5
2	29938.5
3	44484.0
4	55375.0
5	91062.5
6	123242.5
7	145340.0
8	157744.5
9	147256.0
10	118673.0
11	89091.5
12	90773.0
13	127359.5
14	222736.5
15	356933.5
16	464330.5
17	521220.5
18	527592.5
19	492825.5
20	436279.0
21	382399.0
22	358616.5
23	385614.5
24	465830.5
25	595993.0
26	766510.0
27	953132.0
28	1135915.5
29	1331726.5
30	1531036.0
31	1726589.0
32	1938032.0
33	2143595.0
34	2342731.5
35	2525786.5
36	2715482.5
37	2887057.0
38	3001287.0
39	3102353.0
40	3217802.5
41	3316986.5
42	3384274.5
43	3456218.5
44	3510486.5
45	3549121.0
46	3555964.0
47	3541159.5
48	3512484.0
49	3423509.0
50	3305975.5
51	3162059.0
52	2983680.5
53	2786224.5
54	2581141.5
55	2375592.0
56	2167784.0
57	1949865.5
58	1724964.0
59	1489342.0
60	1234572.0
61	992194.5
62	775590.0
63	589784.5
64	438368.0
65	321102.0
66	236645.0
67	178589.5
68	135166.5
69	99409.0
70	72954.5
71	53061.5
72	37589.0
73	26207.5
74	17786.0
75	11839.0
76	7789.5
77	4948.0
78	3241.5
79	2161.5
80	1472.5
81	1017.0
82	757.5
83	556.0
84	388.5
85	278.5
86	208.0
87	157.5
88	106.5
89	74.0
90	54.5
91	42.5
92	35.5
93	24.0
94	13.5
95	9.0
96	10.0
97	15.5
98	24.5
99	44.5
100	86.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.3397347774925095E-6
3	0.0
4	0.0
5	0.05977671396276738
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.003821956759034014
12-13	7.405260570763792E-4
14-15	0.002535102631413134
16-17	0.00990351237943142
18-19	0.0
20-21	7.780002130433667E-5
22-23	9.770280692324665E-5
24-25	1.4629337014714316E-5
26-27	2.1014349663301535E-4
28-29	0.03359705879458933
30-31	0.048018497553223294
32-33	0.03719636254028054
34-35	0.006724029222110231
36-37	0.009892063651660885
38-39	8.287394040766042E-4
40-41	1.314657696510207E-5
42-43	5.4117277660652956E-6
44-45	5.198836594894364E-4
46-47	4.2251052685589107E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.004610031914780301
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	1.3491871730805642E-6
74-75	2.7199910577573985E-6
76-77	1.3025350162716444E-5
78-79	0.009263391049526922
80-81	0.0
82-83	0.0
84-85	0.0
86-87	7.46679292822052E-5
88-89	1.0951170911885653E-4
90-91	0.0
92-93	9.65539895967384E-6
94-95	0.0
96-97	0.003270319374003476
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2434.0
20-21	9975.0
22-23	18010.0
24-25	23348.0
26-27	40152.0
28-29	79407.0
30-31	158875.0
32-33	208244.0
34-35	275625.0
36-37	380144.0
38-39	488765.0
40-41	504755.0
42-43	593827.0
44-45	736267.0
46-47	720104.0
48-49	628889.0
50-51	551196.0
52-53	510229.0
54-55	538782.0
56-57	569930.0
58-59	583282.0
60-61	585689.0
62-63	548769.0
64-65	569992.0
66-67	608959.0
68-69	632537.0
70-71	642721.0
72-73	594199.0
74-75	587998.0
76-77	621950.0
78-79	665325.0
80-81	686077.0
82-83	660186.0
84-85	646038.0
86-87	679985.0
88-89	765664.0
90-91	826620.0
92-93	868485.0
94-95	1049497.0
96-97	2861854.0
98-99	5477090.0
100-101	5.7277899E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.16025854513981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.42027850798743	61.86469422059918
2	10.634873284343957	15.77369904723496
3	2.904174954315642	6.461230964171028
4	1.1887005984354084	3.526173748509292
5	0.6127931874604412	2.272245060838332
6	0.34120037169264095	1.518210466825443
7	0.23183018289822283	1.203481041160948
8	0.15012061893863993	0.890638713075676
9	0.10513061123063316	0.7016861978885102
>10	0.3983224276883676	4.705081950422704
>50	0.008952823458562931	0.4564016338444484
>100	0.003452141112499784	0.4437863426114951
>500	1.1233793054788184E-4	0.055134958623761686
>1k	4.354883795401268E-5	0.06433145436787094
>5k	1.440366903288256E-5	0.06320419982630444
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.754801083119382E-5	0.0	0.0	0.0
3	0.0	1.754801083119382E-5	0.0	2.3397347774925095E-6	0.0
4	0.0	4.211522599486517E-5	0.0	4.679469554985019E-6	0.0
5	1.1698673887462547E-6	4.445496077235768E-5	0.0	4.679469554985019E-6	0.0
6	1.1698673887462547E-6	4.445496077235768E-5	0.0	7.019204332477529E-6	1.1698673887462547E-6
7	1.1698673887462547E-6	4.445496077235768E-5	0.0	1.6378143442447568E-5	1.1698673887462547E-6
8	1.1698673887462547E-6	4.9134430327342696E-5	0.0	1.9887745608686332E-5	4.679469554985019E-6
9	1.1698673887462547E-6	9.943872804343166E-5	0.0	5.7323502048566484E-5	1.2868541276208802E-5
10-11	1.1698673887462548E-5	1.0002366173780479E-4	0.0	7.136191071352155E-5	1.2868541276208802E-5
12-13	1.2868541276208802E-5	1.0060859543217791E-4	0.0	7.604138026850657E-5	1.2868541276208802E-5
14-15	1.4038408664955056E-5	1.497430257595206E-4	0.0	9.124965632220788E-5	2.3982281469298222E-5
16-17	1.6963077136820694E-5	1.5500742900887874E-4	0.0	1.0996753454214794E-4	3.685082274550702E-5
18-19	1.9302811914313206E-5	1.7138557245132633E-4	0.0	1.1874153995774486E-4	4.211522599486517E-5
20-21	2.1057612997432584E-5	1.8717878219940077E-4	0.0	1.497430257595206E-4	4.2700159689238295E-5
22-23	2.3397347774925096E-5	2.216898701674153E-4	0.0	1.7431024092319196E-4	4.562482816110394E-5
24-25	3.2171353190522E-5	2.579557592185492E-4	0.0	2.1467066583493775E-4	5.3228966187954593E-5
26-27	3.9775491217372664E-5	2.760887037441161E-4	0.0	2.626352287735342E-4	5.790843574293961E-5
28-29	4.0945358606118916E-5	2.784284385216086E-4	0.0	4.2583172950363675E-4	5.8493369437312736E-5
30-31	4.737962924422332E-5	3.205436645164738E-4	0.0	8.896841491415268E-4	6.083310421480525E-5
32-33	4.9134430327342696E-5	3.228833992939663E-4	0.0	0.0018337671318597543	6.902217593602903E-5
34-35	5.55687009654471E-5	3.702630285381896E-4	0.0	0.00313290486706247	7.721124765725281E-5
36-37	5.9663236826058995E-5	4.1939745886553233E-4	0.0	0.005088338207351835	7.721124765725281E-5
38-39	5.9663236826058995E-5	4.480592098898156E-4	0.0	0.008276226841685379	8.072084982349158E-5
40-41	6.20029716035515E-5	4.8257029785783007E-4	0.0	0.012019217551979022	8.306058460098408E-5
42-43	6.4342706381044E-5	5.030429771608895E-4	0.0	0.015476175685724205	8.364551829535721E-5
44-45	6.668244115853652E-5	5.586116781263366E-4	0.0	0.01941804385210471	8.481538568410346E-5
46-47	6.902217593602903E-5	5.942926334830974E-4	0.0	0.02401211308771125	9.066472262783475E-5
48-49	6.902217593602903E-5	6.013118378155749E-4	0.0	0.028698016913334373	9.943872804343166E-5
50-51	6.902217593602903E-5	6.791080191672009E-4	0.0	0.03348277453330656	9.943872804343166E-5
52-53	7.25317781022678E-5	6.849573561109321E-4	0.0	0.03808971231018931	1.1230726931964045E-4
54-55	7.25317781022678E-5	6.878820245827977E-4	0.0	0.04251883024398263	1.327799486226999E-4
56-57	7.25317781022678E-5	6.949012289152753E-4	0.0	0.04698889353638207	1.3862928556643118E-4
58-59	7.25317781022678E-5	6.98410831081514E-4	0.0	0.051603435451291674	1.4272382142704307E-4
60-61	7.25317781022678E-5	7.106944386633498E-4	0.0	0.05643908230267432	1.497430257595206E-4
62-63	7.370164549101405E-5	7.270725821057973E-4	0.0	0.06096880883189981	1.5910196486949065E-4
64-65	7.428657918538718E-5	8.107181004011545E-4	0.0	0.06533475392670084	1.626115670357294E-4
66-67	7.604138026850656E-5	8.230017079829902E-4	0.0	0.0696778866074213	1.6787597028508757E-4
68-69	7.779618135162595E-5	8.469839894522885E-4	0.0	0.07399060273603437	1.6963077136820695E-4
70-71	7.838111504599907E-5	8.616073318116166E-4	0.0	0.07789620501336375	1.7548010831193822E-4
72-73	7.838111504599907E-5	8.651169339778553E-4	0.0	0.08096359730665642	1.8951851697689327E-4
74-75	7.838111504599907E-5	8.902690828358999E-4	0.0	0.08341329961869108	1.8951851697689327E-4
76-77	7.955098243474533E-5	9.534419218281976E-4	0.0	0.08575946866682169	1.9185825175438578E-4
78-79	8.189071721223783E-5	9.856132750187198E-4	0.0	0.08728146613958057	1.9419798653187828E-4
80-81	8.189071721223783E-5	9.879530097962122E-4	0.0	0.0877576021668003	2.053117267249677E-4
82-83	8.247565090661096E-5	9.908776782680778E-4	0.0	0.08818167909522082	2.2461453863928093E-4
84-85	8.306058460098408E-5	9.955571478230628E-4	0.0	0.08833785639161845	2.2519947233365405E-4
86-87	8.306058460098408E-5	0.0010131051586542567	0.0	0.08838640588825142	2.2695427341677343E-4
88	8.306058460098408E-5	0.0010692587933140768	0.0	0.08838816068933453	2.2812414080551968E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	112295	0.0	51.494007	1
GTATCAA	207940	0.0	37.847313	1
TCAACGC	253765	0.0	30.36729	4
ATCAACG	255060	0.0	30.199625	3
CAACGCA	259290	0.0	29.704674	5
TATCAAC	268410	0.0	28.917034	2
AACGCAG	268005	0.0	28.788918	6
ACGCAGA	305920	0.0	25.192808	7
CGCAGAG	311655	0.0	24.733353	8
GCAGAGT	367390	0.0	20.94605	9
GTACATG	245490	0.0	19.537224	1
TACATGG	240565	0.0	19.388506	2
AGAGTAC	330080	0.0	19.321737	10-11
GAGTACT	225755	0.0	19.189217	12-13
ACATGGG	241560	0.0	18.788902	3
CAGAGTA	357640	0.0	18.5779	10-11
AGTACTT	245345	0.0	17.280369	12-13
GTACTTT	256300	0.0	16.835924	14-15
CATGGGG	165950	0.0	16.40814	4
ACTTTTT	300050	0.0	14.580943	16-17
>>END_MODULE