FastQCFastQC Report
Fri 27 May 2016
SRR2138900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75847506
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA988050.050.496391
GTATCAA1817050.037.444321
TCAACGC2201700.030.1659624
ATCAACG2214050.030.013243
CAACGCA2256450.029.4326175
TATCAAC2335750.028.7257182
AACGCAG2328550.028.5691186
ACGCAGA2659750.024.9728267
CGCAGAG2707150.024.5212758
GCAGAGT3199000.020.7079229
GTACATG2209100.020.158821
TACATGG2214600.019.6561532
AGAGTAC2870200.019.15107510-11
GAGTACT1896400.019.11892912-13
ACATGGG2227400.018.9773123
CAGAGTA3133150.018.25685510-11
CATGGGG1515550.016.9145074
AGTACTT2096200.016.82064212-13
GTACTTT2163400.016.7074314-15
GTGGTAT457550.014.3755711
Produced by FastQC (version 0.11.5)