FastQCFastQC Report
Fri 27 May 2016
SRR2138899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83371892
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1121300.050.3593641
GTATCAA2035100.037.5874941
TCAACGC2487650.030.0609324
ATCAACG2509550.029.796893
CAACGCA2544250.029.3944115
TATCAAC2627100.028.7529182
AACGCAG2620000.028.5937776
ACGCAGA3008650.024.867317
CGCAGAG3056200.024.480418
GCAGAGT3602400.020.6816469
GTACATG2499150.020.2238731
TACATGG2488300.019.8340662
GAGTACT2129150.019.12788612-13
ACATGGG2513650.019.0754593
AGAGTAC3242500.019.02592510-11
CAGAGTA3529550.018.27514610-11
AGTACTT2336800.017.00293412-13
CATGGGG1689400.016.9373634
GTACTTT2434050.016.69812414-15
ACTTTTT2849050.014.50677616-17
Produced by FastQC (version 0.11.5)