##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83371892
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2467602270559	33.0	31.0	34.0	30.0	34.0
2	32.286660389091324	34.0	31.0	34.0	30.0	34.0
3	32.38854998036988	34.0	31.0	34.0	30.0	34.0
4	35.97709070822094	37.0	35.0	37.0	35.0	37.0
5	35.913368644674634	37.0	35.0	37.0	35.0	37.0
6	35.98421616724255	37.0	35.0	37.0	35.0	37.0
7	35.922626345099616	37.0	35.0	37.0	35.0	37.0
8	35.925157222052725	37.0	35.0	37.0	35.0	37.0
9	37.616195096064274	39.0	37.0	39.0	35.0	39.0
10-11	37.570085785026926	39.0	37.0	39.0	35.0	39.0
12-13	37.54091314732308	39.0	37.0	39.0	35.0	39.0
14-15	38.88115540786816	40.0	38.0	41.0	35.0	41.0
16-17	38.88758199226185	40.0	38.0	41.0	35.0	41.0
18-19	38.94197991812396	40.0	38.0	41.0	35.0	41.0
20-21	38.959302337664965	40.0	38.0	41.0	35.0	41.0
22-23	38.92769204832048	40.0	38.0	41.0	35.0	41.0
24-25	38.90561536599334	40.0	38.0	41.0	35.0	41.0
26-27	38.82008655568377	40.0	38.0	41.0	34.5	41.0
28-29	38.7379354759043	40.0	38.0	41.0	34.0	41.0
30-31	38.643860655518424	40.0	38.0	41.0	34.0	41.0
32-33	38.56368043995103	40.0	38.0	41.0	34.0	41.0
34-35	38.3556569891318	40.0	38.0	41.0	33.5	41.0
36-37	38.379153479962	40.0	38.0	41.0	34.0	41.0
38-39	38.42387438206093	40.0	38.0	41.0	34.0	41.0
40-41	38.40836219731272	40.0	38.0	41.0	34.0	41.0
42-43	38.33172862122106	40.0	38.0	41.0	33.0	41.0
44-45	38.322833638999896	40.0	38.0	41.0	33.0	41.0
46-47	38.27126113521442	40.0	38.0	41.0	33.0	41.0
48-49	38.21978911115967	40.0	38.0	41.0	33.0	41.0
50-51	38.1431236347955	40.0	37.0	41.0	33.0	41.0
52-53	38.07063891767602	40.0	37.0	41.0	33.0	41.0
54-55	37.95153527513456	40.0	37.0	41.0	33.0	41.0
56-57	37.81950842947044	40.0	36.5	41.0	33.0	41.0
58-59	37.669904483240636	39.5	36.0	41.0	32.5	41.0
60-61	37.50000057967004	39.0	36.0	41.0	32.0	41.0
62-63	37.30016837643184	39.0	35.0	41.0	32.0	41.0
64-65	37.05980788161716	39.0	35.0	41.0	31.5	41.0
66-67	36.694503131670885	38.0	35.0	40.0	31.0	41.0
68-69	36.4714538016955	37.5	35.0	40.0	31.0	41.0
70-71	36.22387806383197	37.0	35.0	39.5	31.0	41.0
72-73	35.831805770540655	36.5	35.0	39.0	31.0	41.0
74-75	35.58841499352587	36.0	34.0	39.0	31.0	40.5
76-77	35.279417621800015	35.5	34.0	38.5	31.0	40.0
78-79	34.945970421433685	35.0	34.0	37.0	30.0	39.0
80-81	34.498235886647734	35.0	34.0	37.0	30.0	39.0
82-83	34.115437071681356	35.0	34.0	36.5	29.5	38.5
84-85	34.03957019036948	35.0	34.0	36.0	29.5	37.0
86-87	34.05613842192792	35.0	34.0	36.0	30.0	37.0
88-89	33.98790984120794	35.0	34.0	35.5	30.0	37.0
90-91	33.68767618362496	35.0	34.0	35.0	30.0	36.0
92-93	33.616522278677145	35.0	34.0	35.0	30.0	36.0
94-95	33.59665875842038	35.0	34.0	35.0	30.0	36.0
96-97	33.60045648888288	35.0	34.0	35.0	30.0	36.0
98-99	33.712320457940976	35.0	34.0	35.0	30.5	35.0
100	33.43634362127031	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	23.0
19	105.0
20	484.0
21	2647.0
22	11613.0
23	39610.0
24	102859.0
25	217846.0
26	392513.0
27	620454.0
28	888541.0
29	1186597.0
30	1515605.0
31	1891849.0
32	2362065.0
33	2988938.0
34	3947970.0
35	5438923.0
36	8396775.0
37	1.5691827E7
38	2.3425232E7
39	1.3483132E7
40	766280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49789346270323	20.782259565370065	13.703030752858531	25.016816219068172
2	14.244534596863891	22.54217644479029	40.26293297985849	22.95035597848733
3	16.944676030621928	27.834191408298615	31.3198805659826	23.90125199509686
4	12.081809298510342	19.505020948786914	39.775594873149814	28.63757487955293
5	12.415327858809434	38.72065599329693	36.083962729237804	12.780053418655834
6	27.86015099669323	39.850289111826804	19.542276910304494	12.747282981175479
7	24.71590425223887	33.70959123729614	24.55701257205486	17.017491938410128
8	24.052660337851034	36.51130167466992	22.02927936432101	17.40675862315803
9	25.650161567642005	16.32799816993478	21.439680174224666	36.58216008819855
10-11	23.26346475818573	27.04830503809265	29.98212571372833	19.706104489993294
12-13	24.599204001656254	24.85984711833436	29.708576259217516	20.832372620791865
14-15	21.87982646560286	25.913179496727313	27.35686493689306	24.850129100776762
16-17	21.226873850635375	28.985492208382276	27.885971357456736	21.901662583525617
18-19	21.306421833392005	28.168217053296573	29.612054383988312	20.913306729323114
20-21	22.195584903866322	27.33161472370595	29.400450003442284	21.072350368985443
22-23	21.891299778967525	27.255028917270423	29.279368312945508	21.574302990816545
24-25	21.64961067386001	27.444707168424802	29.28071920639625	21.62496295131893
26-27	21.647621848328047	27.557041157590522	29.204533247775384	21.59080374630605
28-29	21.63274932436443	27.55153621039029	29.23840486327856	21.577309601966725
30-31	21.920866234942476	27.386356583818166	29.128250304655882	21.564526876583475
32-33	21.73864885323135	27.558102253982774	29.08506587203677	21.6181830207491
34-35	21.975687283037356	27.612378532144277	28.983029337006105	21.428904847812262
36-37	21.86990807232121	27.53032188879413	28.982914533463887	21.61685550542077
38-39	21.748488768564215	27.613493170409203	28.9987197475525	21.639298313474086
40-41	21.951280169933646	27.522949801519157	28.84943865744638	21.67633137110082
42-43	21.935522078981823	27.6060547710241	28.635788488521698	21.822634661472378
44-45	21.953149071907486	27.7301629703271	28.433981875312114	21.8827060824533
46-47	22.062247892921118	27.732754803901955	28.116515041010103	22.088482262166824
48-49	22.053461648025834	27.85746145275061	28.04115218818673	22.047924711036828
50-51	22.04750912559753	27.96576006593669	27.90518097659807	22.081549831867715
52-53	22.195547194948023	27.870096175673126	27.839785738372374	22.094570891006477
54-55	22.147014025417633	27.793509891222385	27.9300911421909	22.129384941169086
56-57	22.161998849689226	27.873105452834885	27.856889285249352	22.108006412226537
58-59	22.147792756506764	27.915748897287052	27.862010572921143	22.074447773285044
60-61	22.23844117966123	27.861880142571678	27.860114544963338	22.039564132803754
62-63	22.156865954780496	27.968147943703002	27.805043089679334	22.069943011837168
64-65	22.1323517638822	27.905253758562587	27.802530422189896	22.159864055365315
66-67	22.3145497121376	27.95978628256262	27.66852098432697	22.057143020972802
68-69	22.155317944775383	27.915612944634066	27.754362439550317	22.17470667104023
70-71	22.19092672579267	27.694461282246596	27.812017067718052	22.30259492424268
72-73	22.29072110139223	27.773546753010127	27.721830983693717	22.213901161903927
74-75	22.067861741434083	27.864685573753356	27.75095295920146	22.316499725611102
76-77	22.185468630132195	27.815607049811153	27.68016298414996	22.318761335906686
78-79	22.19534190966063	27.86712569805593	27.646969788530377	22.29056260375306
80-81	22.277282218516646	27.894888153569763	27.599050390926756	22.228779236986835
82-83	22.321992737768532	27.839823022954562	27.65123321731923	22.186951021957675
84-85	22.228324073775227	27.820324673132003	27.703468821200033	22.24788243189274
86-87	22.119134222495195	27.83123119891003	27.743381172313736	22.30625340628104
88-89	22.179809269960256	27.823563510401133	27.685687738696345	22.310939480942263
90-91	22.376378796605163	27.857026711251315	27.665657848530227	22.100936643613288
92-93	22.2883673020676	27.83050492891956	27.680444534113935	22.200683234898904
94-95	22.135843832126394	27.891820589848088	27.727657481973317	22.2446780960522
96-97	22.164019357961653	28.054792224069423	27.798890590074716	21.982297827894207
98-99	23.17864816559458	29.948431250481367	28.033522464727206	18.839398119196844
100	22.195019317212594	30.988757206563005	21.80990573568198	25.00631774054242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16635.0
1	16173.5
2	28146.5
3	41403.0
4	52949.5
5	89495.0
6	120240.5
7	136880.0
8	141697.0
9	128173.0
10	105482.5
11	82154.5
12	85916.5
13	122836.0
14	216544.5
15	349728.5
16	458158.0
17	517696.5
18	525954.5
19	490348.5
20	429192.0
21	367183.0
22	333913.0
23	351265.5
24	419967.0
25	535967.0
26	695214.5
27	875582.0
28	1056315.5
29	1251885.5
30	1451144.0
31	1647511.5
32	1850857.0
33	2045079.5
34	2240953.0
35	2424580.0
36	2611736.0
37	2782943.0
38	2906249.5
39	3019399.0
40	3149558.5
41	3263622.0
42	3338222.0
43	3408379.5
44	3459773.0
45	3499488.0
46	3522487.0
47	3516471.5
48	3481989.0
49	3394564.0
50	3283850.0
51	3142860.5
52	2963057.5
53	2763338.5
54	2546774.5
55	2332118.5
56	2124027.5
57	1909561.0
58	1690265.0
59	1463912.5
60	1221955.5
61	988346.5
62	779390.0
63	600714.5
64	452511.5
65	338389.5
66	253689.0
67	192882.5
68	147131.5
69	108720.0
70	79715.5
71	58511.0
72	42617.5
73	30775.0
74	21757.0
75	14835.5
76	9746.5
77	6116.5
78	3998.0
79	2688.0
80	1837.5
81	1233.0
82	891.5
83	646.5
84	458.5
85	332.5
86	250.5
87	197.0
88	150.0
89	108.5
90	76.5
91	60.0
92	48.5
93	28.0
94	14.0
95	13.0
96	14.0
97	17.5
98	24.5
99	47.5
100	89.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06005021452553818
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0038963971214663086
12-13	7.352597923530391E-4
14-15	0.002564413435645673
16-17	0.009800065470506535
18-19	0.0
20-21	8.516492346363272E-5
22-23	8.157877630239963E-5
24-25	1.4399485304797265E-5
26-27	2.4067016738730176E-4
28-29	0.033314125540536324
30-31	0.04772681939882209
32-33	0.03721159455153277
34-35	0.006726113498210726
36-37	0.009931479341968088
38-39	8.503287535540999E-4
40-41	1.778595271955282E-5
42-43	8.024717760336829E-6
44-45	5.363240054020645E-4
46-47	4.2329527014952213E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.004685033634787696
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	6.933895870135564E-7
74-75	1.3979106547771569E-6
76-77	1.0570060559964038E-5
78-79	0.009386393727056864
80-81	0.0
82-83	0.0
84-85	0.0
86-87	8.045273306717601E-5
88-89	1.0513132109897036E-4
90-91	0.0
92-93	1.9853395805759385E-5
94-95	0.0
96-97	0.0032481341000171102
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2456.0
20-21	10160.0
22-23	18271.0
24-25	23790.0
26-27	39910.0
28-29	80545.0
30-31	165236.0
32-33	213707.0
34-35	280075.0
36-37	388546.0
38-39	499269.0
40-41	511593.0
42-43	598303.0
44-45	739212.0
46-47	716488.0
48-49	621570.0
50-51	543549.0
52-53	500571.0
54-55	528931.0
56-57	559039.0
58-59	572572.0
60-61	574115.0
62-63	537922.0
64-65	555498.0
66-67	591839.0
68-69	618210.0
70-71	625147.0
72-73	578819.0
74-75	572895.0
76-77	608457.0
78-79	647909.0
80-81	668433.0
82-83	642858.0
84-85	632281.0
86-87	664617.0
88-89	745045.0
90-91	804266.0
92-93	845637.0
94-95	1023849.0
96-97	2802685.0
98-99	5356895.0
100-101	5.5660722E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.8853624124298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.1360778501978	61.425392415098415
2	10.730713344354262	15.856852887830225
3	2.982106411527023	6.6100203890431395
4	1.2171196484376985	3.5970930529643397
5	0.6525979824103109	2.4108719220003154
6	0.38005470933802366	1.6848287961594326
7	0.23422983254083274	1.211430924555752
8	0.14858867981598078	0.8782822766870587
9	0.1091066267573049	0.7255244393605063
>10	0.39623365149140877	4.530749671403825
>50	0.009218409036539957	0.46256650523157145
>100	0.003777005207748663	0.47224505596443783
>500	1.3555865493625888E-4	0.06015236104660852
>1k	3.5281567366310295E-5	0.049598196295783895
>5k	5.008662637495296E-6	0.024391106358538236
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1994450119951697E-6	1.1994450119951697E-6
2	1.1994450119951697E-6	2.8786680287884075E-5	0.0	1.1994450119951697E-6	2.3988900239903394E-6
3	1.1994450119951697E-6	2.8786680287884075E-5	0.0	1.0795005107956529E-5	2.3988900239903394E-6
4	1.1994450119951697E-6	7.91633707916812E-5	0.0	1.9191120191922715E-5	2.3988900239903394E-6
5	4.797780047980679E-6	8.036281580367638E-5	0.0	2.1590010215913058E-5	2.3988900239903394E-6
6	5.997225059975849E-6	8.156226081567154E-5	0.0	2.1590010215913058E-5	5.997225059975849E-6
7	5.997225059975849E-6	8.276170582766672E-5	0.0	2.3988900239903397E-5	7.196670071971019E-6
8	7.196670071971019E-6	9.595560095961359E-5	0.0	3.838224038384543E-5	7.196670071971019E-6
9	8.396115083966188E-6	1.703211917033141E-4	0.0	6.3570585635744E-5	8.396115083966188E-6
10-11	2.1590010215913058E-5	1.703211917033141E-4	0.0	7.316614573170535E-5	8.995837589963773E-6
12-13	2.5188345251898566E-5	1.703211917033141E-4	0.0	8.935865339364016E-5	1.0195282601958944E-5
14-15	2.9986125299879244E-5	2.3928927989303638E-4	0.0	1.1454699864553872E-4	1.6792230167932376E-5
16-17	3.838224038384543E-5	2.4528650495301223E-4	0.0	1.3373811883746144E-4	2.099028770991547E-5
18-19	4.37797429378237E-5	2.6627679266292767E-4	0.0	1.5352896153538175E-4	3.058584780587683E-5
20-21	4.617863296181404E-5	2.9866180798679724E-4	0.0	1.8111619681127064E-4	3.598335035985509E-5
22-23	4.7977800479806793E-5	3.610329486105461E-4	0.0	2.063045420631692E-4	3.778251787784785E-5
24-25	6.357058563574399E-5	4.1920603169231184E-4	0.0	2.3149288731506777E-4	4.198057541983094E-5
26-27	7.496531324969811E-5	4.4739298947419834E-4	0.0	2.8007041030087216E-4	4.3180020431826116E-5
28-29	7.736420327368845E-5	4.479927119801959E-4	0.0	4.306007593062659E-4	4.4979187949818866E-5
30-31	7.91633707916812E-5	5.037669050379713E-4	0.0	0.0010003371400039715	4.5578910455816455E-5
32-33	8.216198332166913E-5	5.091644075919497E-4	0.0	0.002188987146891185	4.7977800479806793E-5
34-35	9.355671093562323E-5	5.859288883596405E-4	0.0	0.0037506645525088957	4.917724549180196E-5
36-37	1.0435171604357977E-4	6.584953115853483E-4	0.0	0.00617354347673914	5.037669050379713E-5
38-39	1.0555116105557494E-4	6.938789394392057E-4	0.0	0.010180289539309004	5.277558052778747E-5
40-41	1.151467211515363E-4	7.7724036777287E-4	0.0	0.014789156997900444	5.517447055177781E-5
42-43	1.151467211515363E-4	8.024287130247686E-4	0.0	0.019088567643397128	5.637391556377298E-5
44-45	1.2054422370551457E-4	8.845906963464377E-4	0.0	0.023825775718272053	5.757336057576815E-5
46-47	1.265414487654904E-4	9.439632244401987E-4	0.0	0.02905535597057099	5.817308308176574E-5
48-49	1.2894033878948076E-4	9.499604495001745E-4	0.0	0.03438748877139552	6.477003064773917E-5
50-51	1.2954006129547833E-4	0.0010741030082416746	0.0	0.040029078385314804	6.477003064773917E-5
52-53	1.337381188374614E-4	0.001079500510795653	0.0	0.0453690075787173	6.836836568372467E-5
54-55	1.355372863554542E-4	0.0010902955159036094	0.0	0.05046664887969677	7.436559074370052E-5
56-57	1.355372863554542E-4	0.0011172830286735008	0.0	0.055872547548759	8.156226081567154E-5
58-59	1.355372863554542E-4	0.0011232802537334764	0.0	0.06162388638127584	8.396115083966188E-5
60-61	1.3733645387344694E-4	0.0011394727613954112	0.0	0.06733384436087884	8.636004086365223E-5
62-63	1.379361763794445E-4	0.0011628619391293171	0.0	0.07273734413991709	9.595560095961359E-5
64-65	1.391356213914397E-4	0.0012840058853408292	0.0	0.07788116407385837	9.715504597160875E-5
66-67	1.4033506640343487E-4	0.001303197005532752	0.0	0.0831137429386873	9.775476847760634E-5
68-69	1.4393340143942037E-4	0.001352374251024554	0.0	0.08827435510279652	1.0015365850159668E-4
70-71	1.4393340143942037E-4	0.0013853589888544213	0.0	0.09260255242858109	1.0255254852558702E-4
72-73	1.4393340143942037E-4	0.001398552883986368	0.0	0.09599398319999743	1.0914949609156044E-4
74-75	1.4393340143942037E-4	0.001444731516948182	0.0	0.09876829951274227	1.1034894110355562E-4
76-77	1.475317364754059E-4	0.0015268935002698512	0.0	0.10139628353402368	1.1214810862154837E-4
78-79	1.4993062649939622E-4	0.0015694737981956797	0.0	0.1030797046083589	1.151467211515363E-4
80-81	1.4993062649939622E-4	0.00157187268821967	0.0	0.10361225819368475	1.2234339122350733E-4
82-83	1.5053034900539382E-4	0.0015754710232556556	0.0	0.10404105978547303	1.2954006129547833E-4
84-85	1.5232951652338657E-4	0.0015868657508696096	0.0	0.1042227757047903	1.307395063074735E-4
86-87	1.5232951652338657E-4	0.0016180513211814839	0.0	0.10426415655770413	1.349375638494566E-4
88	1.5232951652338657E-4	0.001737995822381001	0.0	0.1042701537827641	1.379361763794445E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	112130	0.0	50.359364	1
GTATCAA	203510	0.0	37.587494	1
TCAACGC	248765	0.0	30.060932	4
ATCAACG	250955	0.0	29.79689	3
CAACGCA	254425	0.0	29.394411	5
TATCAAC	262710	0.0	28.752918	2
AACGCAG	262000	0.0	28.593777	6
ACGCAGA	300865	0.0	24.86731	7
CGCAGAG	305620	0.0	24.48041	8
GCAGAGT	360240	0.0	20.681646	9
GTACATG	249915	0.0	20.223873	1
TACATGG	248830	0.0	19.834066	2
GAGTACT	212915	0.0	19.127886	12-13
ACATGGG	251365	0.0	19.075459	3
AGAGTAC	324250	0.0	19.025925	10-11
CAGAGTA	352955	0.0	18.275146	10-11
AGTACTT	233680	0.0	17.002934	12-13
CATGGGG	168940	0.0	16.937363	4
GTACTTT	243405	0.0	16.698124	14-15
ACTTTTT	284905	0.0	14.506776	16-17
>>END_MODULE