FastQCFastQC Report
Fri 27 May 2016
SRR2138898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42274840
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA563800.049.557471
GTATCAA1018900.037.0491681
TCAACGC1253200.029.5498454
ATCAACG1257000.029.5246283
CAACGCA1281100.028.8858815
TATCAAC1319850.028.2892072
AACGCAG1323950.028.0113436
ACGCAGA1510300.024.5099017
CGCAGAG1531850.024.1622458
GCAGAGT1794100.020.342099
GTACATG1312550.019.8734341
TACATGG1294000.019.6529752
ACATGGG1318250.018.8888033
GAGTACT1033400.018.88317712-13
AGAGTAC1619550.018.83199310-11
CAGAGTA1744800.018.17866910-11
CTTATAC267250.017.445881
CATGGGG845400.016.7109014
AGTACTT1138600.016.55398212-13
GTACTTT1182300.016.50481214-15
Produced by FastQC (version 0.11.5)