##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42274840
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610407514256707	31.0	31.0	34.0	28.0	34.0
2	31.651467373974686	33.0	31.0	34.0	28.0	34.0
3	31.74584187663395	33.0	31.0	34.0	28.0	34.0
4	35.26645676246202	37.0	35.0	37.0	32.0	37.0
5	35.09333125802487	37.0	35.0	37.0	32.0	37.0
6	35.139325731333344	37.0	35.0	37.0	32.0	37.0
7	35.018342115546744	37.0	35.0	37.0	32.0	37.0
8	35.041818419655755	37.0	35.0	37.0	32.0	37.0
9	36.5623161672522	39.0	37.0	39.0	32.0	39.0
10-11	36.51282908462811	39.0	37.0	39.0	32.0	39.0
12-13	36.47942821309318	39.0	37.0	39.0	32.0	39.0
14-15	37.67408790902579	40.0	37.0	41.0	32.0	41.0
16-17	37.697397920370605	40.0	37.0	41.0	32.0	41.0
18-19	37.75639349551648	40.0	37.0	41.0	32.0	41.0
20-21	37.78487450877045	40.0	37.0	41.0	32.0	41.0
22-23	37.747551505139484	40.0	37.0	41.0	32.0	41.0
24-25	37.740011960263956	40.0	37.0	41.0	32.0	41.0
26-27	37.671327560430186	40.0	37.0	41.0	31.0	41.0
28-29	37.617979732554716	40.0	37.0	41.0	31.0	41.0
30-31	37.52423896086241	40.0	37.0	41.0	31.0	41.0
32-33	37.44765323941715	40.0	36.0	41.0	31.0	41.0
34-35	37.35823900898382	40.0	36.0	41.0	30.5	41.0
36-37	37.32577897899128	40.0	36.0	41.0	30.0	41.0
38-39	37.26802861180481	40.0	36.0	41.0	30.0	41.0
40-41	37.25199361387961	40.0	36.0	41.0	30.0	41.0
42-43	37.21820040831008	40.0	36.0	41.0	30.0	41.0
44-45	37.14719330357643	40.0	36.0	41.0	30.0	41.0
46-47	37.07411634760898	40.0	36.0	41.0	30.0	41.0
48-49	37.01798279288771	39.0	35.5	41.0	30.0	41.0
50-51	36.94586794783006	39.0	35.0	41.0	30.0	41.0
52-53	37.164822550297714	39.0	36.0	41.0	30.0	41.0
54-55	37.15836302234577	39.0	35.5	41.0	30.0	41.0
56-57	37.07918080678661	39.0	35.0	41.0	30.0	41.0
58-59	36.94744385353652	39.0	35.0	41.0	30.0	41.0
60-61	36.82690554569007	39.0	35.0	41.0	30.0	41.0
62-63	36.67131385800033	39.0	35.0	41.0	30.0	41.0
64-65	36.475986145563354	38.0	35.0	40.5	30.0	41.0
66-67	36.25201720299116	38.0	34.5	40.0	30.0	41.0
68-69	35.99986776015143	37.0	34.0	40.0	29.0	41.0
70-71	35.75177930068311	37.0	34.0	39.5	29.0	41.0
72-73	35.47574447377883	36.0	34.0	39.0	29.0	41.0
74-75	35.206955191258615	36.0	34.0	39.0	29.0	41.0
76-77	34.90838576594983	35.5	34.0	38.5	29.0	40.0
78-79	34.62998201386234	35.0	34.0	37.0	29.0	39.0
80-81	34.36493629003283	35.0	34.0	37.0	29.0	39.0
82-83	34.10052824999412	35.0	33.0	36.5	29.0	39.0
84-85	33.88338472597394	35.0	33.0	36.0	29.0	37.0
86-87	33.6570864494292	35.0	33.0	36.0	29.0	37.0
88-89	33.510687162409184	35.0	33.0	35.0	29.0	37.0
90-91	33.4076403817096	35.0	33.0	35.0	29.0	36.0
92-93	33.33313474649971	35.0	33.0	35.0	29.0	36.0
94-95	33.38464458712092	35.0	33.0	35.0	29.0	36.0
96-97	33.56243577742437	35.0	33.5	35.0	30.0	36.0
98-99	33.656705254984786	35.0	34.0	35.0	30.0	36.0
100	32.86396636285536	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	11.0
14	14.0
15	40.0
16	133.0
17	559.0
18	4351.0
19	22091.0
20	68752.0
21	151502.0
22	251264.0
23	344452.0
24	428684.0
25	507783.0
26	579957.0
27	650467.0
28	733067.0
29	834604.0
30	959146.0
31	1123001.0
32	1345619.0
33	1659310.0
34	2132077.0
35	2841658.0
36	4171965.0
37	7176735.0
38	1.0245571E7
39	5785861.0
40	256165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.75841185915783	21.32434327368241	14.561888347773758	24.355356519386
2	14.167038233433704	22.760667227058825	40.456018469614605	22.616276069892862
3	16.855190936263746	28.047117387079407	31.598052174768725	23.49963950188812
4	11.990398273775437	19.96556323727549	39.88945305574044	28.154585433208627
5	12.23455554600605	38.952354453853864	36.29392262704135	12.519167373098739
6	27.366914174038325	40.382678560677824	19.71473629092361	12.535670974360237
7	24.456406827877416	33.823495249818215	24.726091530755856	16.99400639154851
8	23.744721243476643	36.87401527828721	22.19545413450534	17.185809343730806
9	25.378134590740736	16.48823600460022	21.61869550763205	36.514933897027
10-11	23.055645638356374	27.311527494594223	30.174523181398964	19.45830368565043
12-13	24.417545000969962	25.062327304077126	29.88065517312425	20.63947252182866
14-15	21.668042327938448	26.171170850979724	27.502923400940283	24.65786342014155
16-17	21.01868235776509	29.292033850199584	28.015915062328283	21.67336872970704
18-19	21.123036108282825	28.424475372514515	29.736008751969067	20.716479767233597
20-21	22.068089443586068	27.571009352275876	29.477900720796214	20.883000483341846
22-23	21.690213340157317	27.584876247752643	29.384801272758732	21.34010913933131
24-25	21.46034740463644	27.755455767225662	29.433204216340926	21.350992611796975
26-27	21.465309300039763	27.857969671638706	29.305861626686724	21.370859401634807
28-29	21.405831450941033	27.85158394660432	29.35802377988182	21.38456082257283
30-31	21.737256706405255	27.70709554809066	29.26389710991032	21.29175063559377
32-33	21.44376559274881	27.92806964013282	29.21184719854019	21.41631756857818
34-35	21.596429707172454	27.922723890157563	29.187109513184993	21.293736889484986
36-37	21.57664950932254	27.88047490571193	29.12106885354978	21.421806731415746
38-39	21.48530228758071	27.885260259971822	29.187624761880958	21.44181269056651
40-41	21.663756407923167	27.79893320369898	29.053545015164044	21.48376537321381
42-43	21.654287410086322	27.88385382550212	28.877504589864472	21.584354174547087
44-45	21.671484054420887	28.020527934205614	28.676320685582883	21.631667325790612
46-47	21.842994416899927	28.000586651640663	28.318146240948934	21.83827269051048
48-49	21.77274135396991	28.107858282255	28.304952005718775	21.814448358056314
50-51	21.72536093454499	28.229776482022373	28.188601775550225	21.856260807882414
52-53	21.881190014021655	28.161083949169875	28.172291617258672	21.785434419549798
54-55	21.839527766655056	28.08494798037671	28.217110089939613	21.85841416302862
56-57	21.88479826270564	28.194654733015685	28.099091506475798	21.821455497802873
58-59	21.82285596306968	28.257482543493612	28.16630093180325	21.753360561633453
60-61	21.908818372445317	28.274423592552928	28.075503807835528	21.74125422716623
62-63	21.793135825095593	28.387720685831564	28.055321462039256	21.763822027033587
64-65	21.78950085952006	28.29247978466392	28.068769138749865	21.84925021706615
66-67	21.88479453415009	28.310008258268148	27.972725886073395	21.83247132150836
68-69	21.85316343223607	28.263629062557733	28.01487609206451	21.86833141314169
70-71	21.88978178837446	28.013493614399398	28.10306870484615	21.993655892379987
72-73	21.907578124468355	28.08287110133465	28.039394091856778	21.97015668234022
74-75	21.754999798941498	28.189675005210006	28.053318098926383	22.00200709692211
76-77	21.877990310464774	28.16739019138725	27.989456343716025	21.965163154431956
78-79	21.862369195122984	28.19108364864535	27.95250060525758	21.994046550974087
80-81	21.867431146894774	28.175069921742786	27.94025893007693	22.01724000128552
82-83	21.87528507129648	28.146532860151453	28.02263903906591	21.955543029486158
84-85	21.9192745016297	28.133069329631784	28.014584371561746	21.933071797176773
86-87	21.808571329957534	28.159183737670347	28.06336213075527	21.968882801616846
88-89	21.89799907600766	28.152295770740555	27.98604827928542	21.963656873966368
90-91	21.870865832695003	28.189074041226984	28.050293282114858	21.889766843963155
92-93	21.88729657007358	28.178256303750874	27.999055176091307	21.935391950084234
94-95	21.755546295303265	28.21412122767224	28.059992214538944	21.970340262485553
96-97	21.782392013085	28.361927049873042	28.135874607640606	21.719806329401354
98-99	22.81365542987285	30.307813060640232	28.359902044489576	18.518629464997346
100	21.890176976398763	31.35859804877377	22.074159474734465	24.677065500092997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7335.0
1	7642.0
2	12742.0
3	17861.5
4	22376.5
5	37449.0
6	50757.0
7	58997.5
8	63158.5
9	58886.0
10	49321.0
11	39194.5
12	40866.0
13	57725.0
14	101151.5
15	164733.5
16	217102.0
17	245808.5
18	252695.0
19	241462.0
20	220064.0
21	200977.5
22	196222.5
23	215786.5
24	260774.5
25	329415.5
26	420083.0
27	519867.0
28	617912.0
29	724013.0
30	830263.5
31	931550.0
32	1036591.0
33	1136779.0
34	1237379.5
35	1330093.0
36	1420391.5
37	1501452.0
38	1557146.5
39	1608652.5
40	1669330.5
41	1714986.5
42	1741875.5
43	1772198.5
44	1800795.0
45	1819435.5
46	1808848.0
47	1780909.0
48	1748334.5
49	1698286.0
50	1643583.0
51	1571568.5
52	1477782.0
53	1372910.0
54	1262920.5
55	1157486.0
56	1051360.5
57	941978.5
58	830591.5
59	720835.0
60	606288.0
61	492237.5
62	389797.0
63	302928.0
64	230478.5
65	172608.0
66	129893.0
67	100515.0
68	78994.0
69	59988.0
70	44621.0
71	33296.0
72	24854.5
73	18316.5
74	13551.0
75	10089.0
76	7343.5
77	5178.5
78	3770.5
79	2778.0
80	2057.0
81	1553.5
82	1211.5
83	852.5
84	593.5
85	452.5
86	357.5
87	279.0
88	186.5
89	122.5
90	85.5
91	64.5
92	50.0
93	26.0
94	9.5
95	7.5
96	6.0
97	4.0
98	3.5
99	6.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013319979448769056
3	0.0
4	0.12698333098363
5	0.03673106746234876
6	0.004790083179498728
7	0.005066843540980876
8	4.8728747406258664E-4
9	8.089918258708962E-4
10-11	0.004738042769647384
12-13	6.85987220767719E-5
14-15	4.6126726913691456E-5
16-17	4.0331317634791753E-4
18-19	2.4837468338141553E-5
20-21	8.889973310429795E-4
22-23	0.030158011649949714
24-25	0.001001448778386742
26-27	0.0044685586345111356
28-29	5.497916682285748E-4
30-31	0.0025942659946607263
32-33	0.003420864755124773
34-35	0.019813337204013282
36-37	0.0043558867825531184
38-39	0.001582076643994198
40-41	9.893478988143825E-4
42-43	9.27497678672587E-4
44-45	0.030565831628194633
46-47	0.003907266962843933
48-49	0.005739282547205852
50-51	0.01941717801223997
52-53	0.004314701849095582
54-55	9.670651608634676E-4
56-57	0.0012609773418524686
58-59	0.004034816858769948
60-61	0.00289399781147042
62-63	8.635370198720822E-4
64-65	1.5336537306382607E-4
66-67	1.3566834841151358E-6
68-69	3.6410687034896244E-4
70-71	0.01335059671515905
72-73	0.004109355370707848
74-75	0.0030520016496561175
76-77	7.09402823709837E-6
78-79	0.0
80-81	8.533472553322162E-5
82-83	1.0228410040125468E-5
84-85	2.8040276167204166E-5
86-87	0.0055653049852728885
88-89	0.0
90-91	8.38756452972771E-5
92-93	1.1893695141393097E-4
94-95	2.975605888955838E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24821.0
20-21	48328.0
22-23	49979.0
24-25	53964.0
26-27	62721.0
28-29	84866.0
30-31	127390.0
32-33	145322.0
34-35	176926.0
36-37	222472.0
38-39	272933.0
40-41	281420.0
42-43	326124.0
44-45	387514.0
46-47	373545.0
48-49	330293.0
50-51	290296.0
52-53	272488.0
54-55	285888.0
56-57	300325.0
58-59	310291.0
60-61	310672.0
62-63	295070.0
64-65	307245.0
66-67	323458.0
68-69	337805.0
70-71	337150.0
72-73	309683.0
74-75	306139.0
76-77	320841.0
78-79	340839.0
80-81	354047.0
82-83	340184.0
84-85	338990.0
86-87	352685.0
88-89	383051.0
90-91	413123.0
92-93	433785.0
94-95	499681.0
96-97	1369045.0
98-99	2715938.0
100-101	2.7457503E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.97175864272259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.97510098597456	69.55551783976782
2	8.452853483854435	13.519791173062073
3	2.3395672226854227	5.612979157830704
4	0.958274776672448	3.065396766138316
5	0.47482165971923246	1.8986161584701708
6	0.25927661268389157	1.2440884014755305
7	0.14389962963916048	0.8055534515196078
8	0.10073155643689827	0.6444543775261934
9	0.06788894579065423	0.48862785485511695
>10	0.22150284575257442	2.6348015414824464
>50	0.004652362884260688	0.24844430021995692
>100	0.0013463841468002945	0.1782861467764432
>500	3.287604939207938E-5	0.018061122741617707
>1k	5.065771030678757E-5	0.08538170813387835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.41928390503666E-5
2	0.0	3.0751151275794304E-5	0.0	2.3654731750611E-6	1.41928390503666E-5
3	0.0	3.0751151275794304E-5	0.0	9.4618927002444E-6	1.41928390503666E-5
4	0.0	8.51570343021996E-5	0.0	2.12892585755499E-5	1.41928390503666E-5
5	2.3654731750611E-6	8.98879806523218E-5	0.0	2.6020204925672102E-5	1.41928390503666E-5
6	4.9674936676283105E-5	9.225345382738291E-5	0.0	2.6020204925672102E-5	2.6020204925672102E-5
7	4.9674936676283105E-5	9.225345382738291E-5	0.0	3.78475708009776E-5	2.6020204925672102E-5
8	5.2040409851344205E-5	1.0171534652762731E-4	0.0	3.78475708009776E-5	2.6020204925672102E-5
9	5.4405883026405304E-5	1.916033271799491E-4	0.0	1.159081855779939E-4	3.3116624450855404E-5
10-11	5.913682937652751E-5	1.916033271799491E-4	0.0	1.3364923439095216E-4	3.903030738850815E-5
12-13	6.386777572664971E-5	1.916033271799491E-4	0.0	1.537557563789715E-4	4.6126726913691456E-5
14-15	6.741598548924135E-5	2.6493299560684323E-4	0.0	1.608521759041548E-4	5.913682937652751E-5
16-17	1.159081855779939E-4	2.767603614821487E-4	0.0	1.797759613046436E-4	6.978145866430246E-5
18-19	1.2300460510317722E-4	3.07511512757943E-4	0.0	1.963342735300713E-4	7.56951416019552E-5
20-21	1.253700782782383E-4	3.4299361038385955E-4	0.0	2.140753223430296E-4	8.04260879520774E-5
22-23	1.41928390503666E-4	4.092268592855703E-4	0.0	2.3654731750611004E-4	8.397429771466906E-5
24-25	1.6321764907921592E-4	4.5890179596185345E-4	0.0	2.767603614821487E-4	8.51570343021996E-5
26-27	1.726795417794603E-4	4.884702106501172E-4	0.0	3.3471445427114566E-4	8.51570343021996E-5
28-29	1.750450149545214E-4	4.932011570002394E-4	0.0	5.334142009762781E-4	8.51570343021996E-5
30-31	1.8332417106723526E-4	5.819064010650307E-4	0.0	0.0013317613975593993	8.51570343021996E-5
32-33	1.9278606376747965E-4	5.901855571777445E-4	0.0	0.002865770751586523	1.0881176605281061E-4
34-35	2.081616394053768E-4	6.717943817173525E-4	0.0	0.004894163999201416	1.18273658753055E-4
36-37	2.105271125804379E-4	7.640478355447353E-4	0.0	0.007775310326425837	1.2300460510317722E-4
38-39	2.1170984916796845E-4	7.995299331706519E-4	0.0	0.01251571856924828	1.3246649780342161E-4
40-41	2.1289258575549902E-4	8.764078113601376E-4	0.0	0.018011895491502748	1.3601470756601327E-4
42-43	2.1289258575549902E-4	9.142553821611151E-4	0.0	0.0232017436375868	1.3956291732860493E-4
44-45	2.1407532234302956E-4	0.0010183362018638036	0.0	0.02900543207259921	1.4311112709119655E-4
46-47	2.152580589305601E-4	0.0010928486068782282	0.0	0.035149748644820414	1.466593368537882E-4
48-49	2.1998900528068233E-4	0.0010952140800532893	0.0	0.04132481636831742	1.5730396614156315E-4
50-51	2.4719194679388495E-4	0.0012217668949190581	0.0	0.04746321925760097	1.608521759041548E-4
52-53	2.507401565564766E-4	0.001238325207144486	0.0	0.05348216575154394	1.632176490792159E-4
54-55	2.507401565564766E-4	0.0012477870998447303	0.0	0.058811576814956606	1.6794859542933812E-4
56-57	2.507401565564766E-4	0.0012702590950078108	0.0	0.06283169847597295	1.703140686043992E-4
58-59	2.519228931440071E-4	0.0012761727779454636	0.0	0.06691687064930346	1.7386227836699086E-4
60-61	2.625675224317821E-4	0.001301010246283605	0.0	0.07116171226195062	1.750450149545214E-4
62-63	2.885877273574542E-4	0.0013400405536721132	0.0	0.07522322970353051	1.797759613046436E-4
64-65	2.885877273574542E-4	0.0014417559001997406	0.0	0.07884950008089918	1.8095869789217417E-4
66-67	3.015978298202903E-4	0.0014701415783004738	0.0	0.08263898810734707	1.8450690765476583E-4
68-69	3.051460395828819E-4	0.0015198165149767568	0.0	0.08644739991919544	1.868723808298269E-4
70-71	3.1579066887065687E-4	0.0015517504028400818	0.0	0.08991991454018514	1.9160332717994914E-4
72-73	3.394454006212679E-4	0.0015659432418904484	0.0	0.09268751815500662	2.057961662303157E-4
74-75	3.406281372087984E-4	0.0016144354419792009	0.0	0.09485429158336259	2.057961662303157E-4
76-77	3.4417634697139006E-4	0.001724429944619542	0.0	0.09688623304074007	2.0816163940537682E-4
78-79	3.4535908355892063E-4	0.0017634602520080502	0.0	0.09815767487233541	2.0816163940537682E-4
80-81	3.4535908355892063E-4	0.0017658257251831112	0.0	0.09860829751218456	2.1762353210562122E-4
82-83	3.4535908355892063E-4	0.0017705566715332335	0.0	0.09893355007375546	2.3536458091857947E-4
84-85	3.4535908355892063E-4	0.001782384037408539	0.0	0.09904827552274592	2.4009552726870167E-4
86-87	3.536382396716345E-4	0.0018107697155092723	0.0	0.09909085403989701	2.4364373703129332E-4
88	3.5482097625916503E-4	0.001916033271799491	0.0	0.09909676772283467	2.483746833814155E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	56380	0.0	49.55747	1
GTATCAA	101890	0.0	37.049168	1
TCAACGC	125320	0.0	29.549845	4
ATCAACG	125700	0.0	29.524628	3
CAACGCA	128110	0.0	28.885881	5
TATCAAC	131985	0.0	28.289207	2
AACGCAG	132395	0.0	28.011343	6
ACGCAGA	151030	0.0	24.509901	7
CGCAGAG	153185	0.0	24.162245	8
GCAGAGT	179410	0.0	20.34209	9
GTACATG	131255	0.0	19.873434	1
TACATGG	129400	0.0	19.652975	2
ACATGGG	131825	0.0	18.888803	3
GAGTACT	103340	0.0	18.883177	12-13
AGAGTAC	161955	0.0	18.831993	10-11
CAGAGTA	174480	0.0	18.178669	10-11
CTTATAC	26725	0.0	17.44588	1
CATGGGG	84540	0.0	16.710901	4
AGTACTT	113860	0.0	16.553982	12-13
GTACTTT	118230	0.0	16.504812	14-15
>>END_MODULE