FastQCFastQC Report
Fri 27 May 2016
SRR2138897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30710335
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA372400.049.071571
GTATCAA659700.035.630171
TCAACGC796950.028.9426774
ATCAACG801850.028.7710843
CAACGCA818700.028.116425
TATCAAC849400.027.301022
AACGCAG848400.027.2242186
ACGCAGA972600.023.6728737
CGCAGAG990850.023.2539718
GTACATG879750.019.9136681
TACATGG872500.019.5656682
GCAGAGT1185500.019.1663559
ACATGGG892100.018.5762773
GAGTACT657000.018.5740712-13
AGAGTAC1051600.018.2649210-11
CAGAGTA1148500.017.5973610-11
CATGGGG605450.016.6313484
GTACTTT749600.016.22527714-15
AGTACTT722250.016.06450712-13
GTGGTAT178650.013.8760121
Produced by FastQC (version 0.11.5)