##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138897_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30710335
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606480066075477	31.0	31.0	34.0	28.0	34.0
2	31.65063878332815	33.0	31.0	34.0	28.0	34.0
3	31.745792190153576	33.0	31.0	34.0	28.0	34.0
4	35.26599814036545	37.0	35.0	37.0	32.0	37.0
5	35.08820252204999	37.0	35.0	37.0	32.0	37.0
6	35.1335812520443	37.0	35.0	37.0	32.0	37.0
7	35.0127144493865	37.0	35.0	37.0	32.0	37.0
8	35.03512638334945	37.0	35.0	37.0	32.0	37.0
9	36.56100833807251	39.0	37.0	39.0	32.0	39.0
10-11	36.507869305235516	39.0	37.0	39.0	32.0	39.0
12-13	36.47620377635086	39.0	37.0	39.0	32.0	39.0
14-15	37.676254817799936	40.0	37.0	41.0	32.0	41.0
16-17	37.69505558959223	40.0	37.0	41.0	32.0	41.0
18-19	37.747440706849986	40.0	37.0	41.0	32.0	41.0
20-21	37.771606451398284	40.0	37.0	41.0	32.0	41.0
22-23	37.73567347492537	40.0	37.0	41.0	32.0	41.0
24-25	37.728250884974315	40.0	37.0	41.0	31.5	41.0
26-27	37.66161331260285	40.0	37.0	41.0	31.0	41.0
28-29	37.60811055251527	40.0	37.0	41.0	31.0	41.0
30-31	37.51787273874229	40.0	37.0	41.0	31.0	41.0
32-33	37.44414767438859	40.0	36.0	41.0	31.0	41.0
34-35	37.35640591484806	40.0	36.0	41.0	30.5	41.0
36-37	37.32257965714467	40.0	36.0	41.0	30.0	41.0
38-39	37.26755221636081	40.0	36.0	41.0	30.0	41.0
40-41	37.247636104033134	40.0	36.0	41.0	30.0	41.0
42-43	37.209200620522275	40.0	36.0	41.0	30.0	41.0
44-45	37.13473819786779	40.0	36.0	41.0	30.0	41.0
46-47	37.05829836113327	40.0	36.0	41.0	30.0	41.0
48-49	36.998467119172155	39.0	35.0	41.0	30.0	41.0
50-51	36.922312867580686	39.0	35.0	41.0	30.0	41.0
52-53	37.1329187799729	39.0	36.0	41.0	30.0	41.0
54-55	37.119702266057374	39.0	35.0	41.0	30.0	41.0
56-57	37.03219531381923	39.0	35.0	41.0	30.0	41.0
58-59	36.89499243262644	39.0	35.0	41.0	30.0	41.0
60-61	36.76476603364162	39.0	35.0	41.0	30.0	41.0
62-63	36.5982347951287	38.5	35.0	41.0	30.0	41.0
64-65	36.39246811439389	38.0	35.0	40.0	30.0	41.0
66-67	36.16233766411984	37.5	34.5	40.0	29.5	41.0
68-69	35.902516564389195	37.0	34.0	40.0	29.0	41.0
70-71	35.64629913881119	36.5	34.0	39.0	29.0	41.0
72-73	35.365438578204994	36.0	34.0	39.0	29.0	41.0
74-75	35.09451101349818	36.0	34.0	39.0	29.0	40.5
76-77	34.7967026985808	35.0	34.0	37.5	29.0	40.0
78-79	34.52223872256993	35.0	34.0	37.0	29.0	39.0
80-81	34.2631938710045	35.0	33.5	37.0	29.0	39.0
82-83	34.00652881018618	35.0	33.0	36.0	29.0	38.5
84-85	33.798320886286575	35.0	33.0	36.0	29.0	37.0
86-87	33.579909853981775	35.0	33.0	35.5	29.0	37.0
88-89	33.443551087869494	35.0	33.0	35.0	29.0	36.5
90-91	33.348148291817964	35.0	33.0	35.0	29.0	36.0
92-93	33.281811720259284	35.0	33.0	35.0	29.0	36.0
94-95	33.34064970447529	35.0	33.0	35.0	29.0	36.0
96-97	33.52933182412234	35.0	33.0	35.0	30.0	36.0
98-99	33.62792333022224	35.0	34.0	35.0	30.0	35.0
100	32.82984283803759	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	8.0
14	11.0
15	35.0
16	76.0
17	381.0
18	3345.0
19	16420.0
20	50322.0
21	110748.0
22	183736.0
23	252851.0
24	315550.0
25	373720.0
26	428331.0
27	479232.0
28	539459.0
29	612033.0
30	701501.0
31	820892.0
32	984695.0
33	1214392.0
34	1562502.0
35	2091188.0
36	3097137.0
37	5341902.0
38	7446726.0
39	3922863.0
40	160277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.742679459536994	21.162823525044583	14.526139164551607	24.568357850866818
2	14.365776055466938	22.507801414374665	40.16325188867649	22.963170641481902
3	17.098559817077867	27.645335031350193	31.336701471996314	23.91940367957562
4	12.365163529827525	19.64353303838104	39.387623773884535	28.6036796579069
5	12.57778202111704	38.70626405802934	36.03638713365245	12.679566787201171
6	28.138583017881295	39.77111217674701	19.428130829550653	12.662173975821037
7	24.926133450268487	33.502605347959694	24.488323493903597	17.082937707868222
8	24.060767888367394	36.61563286191182	21.943928211136072	17.379671038584714
9	25.633257040253326	16.359513998025598	21.375775596293607	36.63145336542746
10-11	23.47107695836619	27.01591802333908	29.857114749831954	19.65589026846278
12-13	24.651142503599523	24.778865045328903	29.556677675669864	21.013314775401714
14-15	21.950965962729043	25.88395813062257	27.110452982735882	25.0546229239125
16-17	21.440971682340994	28.916136042222583	27.673905488277335	21.968986787159096
18-19	21.5333466719511	28.196770998395536	29.21641492698458	21.05346740266878
20-21	22.433480368199014	27.362952324175115	28.967809897946	21.23575740967987
22-23	22.124459319612715	27.311457818786256	28.842653643445388	21.721429218155645
24-25	21.853807293660008	27.49401946798951	28.89063397641306	21.761539261937422
26-27	21.82960582798865	27.635488082403352	28.762142242776417	21.772763846831577
28-29	21.79859604782762	27.573280251965038	28.8551995555948	21.772924144612546
30-31	22.065967091809597	27.43982887898596	28.767200426587653	21.72700360261679
32-33	21.889105124678437	27.63093594822943	28.671672454756653	21.808286472335475
34-35	21.98098726997884	27.638579827107396	28.663101971888388	21.717330931025376
36-37	22.051122387429213	27.546997632603688	28.59545463557801	21.80642534438909
38-39	21.905053540221363	27.653563079223463	28.6422680574205	21.799115323134664
40-41	22.088313359061992	27.55832663614612	28.509736282137098	21.843623722654794
42-43	22.021331581862707	27.634587858420208	28.355389632135736	21.98869092758135
44-45	22.071619300784047	27.745138192972707	28.1797488477366	22.003493658506645
46-47	22.19272459678842	27.741364244197026	27.882837799180393	22.183073359834157
48-49	22.140218453532974	27.825361630986407	27.889365305217478	22.14505461026314
50-51	22.12142690381493	27.93484685273891	27.771363069710752	22.172363173735405
52-53	22.254681645700742	27.85481263543025	27.729752684785637	22.160753034083374
54-55	22.234660157874423	27.79918039454956	27.793431864704644	22.172727582871378
56-57	22.231821321282737	27.890003407137286	27.745310184391087	22.13286508718889
58-59	22.188287763768496	27.911643162535015	27.760148169322456	22.13992090437403
60-61	22.279020565970704	27.899889146777852	27.720112091765177	22.10097819548627
62-63	22.205483728923696	27.99095438952466	27.6894918201098	22.114070061441847
64-65	22.173835212617725	27.9033887782065	27.70304832278036	22.219727686395412
66-67	22.27275596589858	27.92225611594778	27.606497829803317	22.198490088350326
68-69	22.22839256856481	27.899445694487074	27.654746883975616	22.2174148529725
70-71	22.248565931275223	27.690480446477554	27.730452753856007	22.330500868391216
72-73	22.254734940180896	27.75647077031761	27.658949724396546	22.32984456510495
74-75	22.10815418812849	27.863224719485313	27.67586609997683	22.352754992409363
76-77	22.22777691613874	27.811572841391907	27.644677526823475	22.31597271564587
78-79	22.211988918543888	27.87900305900456	27.600070989968767	22.30893703248279
80-81	22.236596314951143	27.84149948022113	27.571643303362354	22.350260901465372
82-83	22.22351920053117	27.824071685455788	27.662274801205932	22.290134312807112
84-85	22.275332681176714	27.83145612466804	27.628799091664806	22.264412102490432
86-87	22.182103484327385	27.833152894295488	27.678040803245953	22.306702818131175
88-89	22.2323065173429	27.8330744559786	27.653154899279542	22.28146412739896
90-91	22.219348978683136	27.84313267201351	27.693273682311002	22.24424466699235
92-93	22.245840989363415	27.810928384535906	27.664701251825424	22.27852937427525
94-95	22.078874595585056	27.886810249439286	27.72775078914226	22.3065643658334
96-97	22.137207569943172	28.027434288693758	27.797801595741895	22.037556545621175
98-99	23.21908680635703	29.969785192065668	27.972445375935774	18.83868262564153
100	22.280731239967942	31.00719129387927	21.6476345654497	25.06444290070309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6443.0
1	6526.0
2	8548.5
3	10523.5
4	12854.0
5	21677.0
6	29683.0
7	34526.0
8	36843.0
9	34380.0
10	29252.0
11	23752.0
12	24809.5
13	34427.5
14	60030.0
15	98511.0
16	131257.5
17	150350.0
18	156477.5
19	151158.0
20	138423.0
21	125926.0
22	121168.5
23	131038.5
24	157336.0
25	200015.0
26	257506.0
27	322548.0
28	391507.0
29	467384.5
30	543014.0
31	616676.0
32	693720.5
33	767299.5
34	842039.5
35	916076.5
36	989829.0
37	1054302.5
38	1099482.0
39	1140268.5
40	1185661.5
41	1222198.5
42	1249235.5
43	1273352.5
44	1291199.0
45	1309249.5
46	1315924.0
47	1311663.0
48	1299408.0
49	1271330.5
50	1235672.0
51	1188415.0
52	1126333.5
53	1054171.0
54	978224.0
55	901605.5
56	824563.5
57	746480.5
58	665774.0
59	580581.0
60	487152.5
61	396438.0
62	316717.0
63	248076.0
64	189659.5
65	144095.0
66	109884.0
67	84921.0
68	66216.5
69	50220.0
70	37699.5
71	28454.0
72	21581.0
73	16179.5
74	11834.5
75	8664.0
76	6255.5
77	4392.5
78	3204.5
79	2391.5
80	1782.5
81	1366.5
82	1088.0
83	754.5
84	497.5
85	344.5
86	259.0
87	203.5
88	142.5
89	101.5
90	81.0
91	69.5
92	57.0
93	33.0
94	12.0
95	6.5
96	6.0
97	5.5
98	4.5
99	5.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01316820542660964
3	0.0
4	0.1282499848992204
5	0.03660656909148012
6	0.0048615555642750235
7	0.005135079119130416
8	5.470471097107863E-4
9	8.270831301579745E-4
10-11	0.004874580495458613
12-13	5.2099724734360595E-5
14-15	4.721537554051429E-5
16-17	4.3633519465027E-4
18-19	2.116551317333399E-5
20-21	8.587751444921772E-4
22-23	0.02990346462040326
24-25	0.0010666427442493052
26-27	0.004519216606967919
28-29	5.815501653773044E-4
30-31	0.002677593260975493
32-33	0.0034758617210330026
34-35	0.019888586576572043
36-37	0.00449948482391958
38-39	0.0016314859846262306
40-41	0.0010173264117980855
42-43	9.362510702245425E-4
44-45	0.030167104123744334
46-47	0.003935968428101914
48-49	0.005747589245102685
50-51	0.019052707554090286
52-53	0.004318774590616305
54-55	9.737174711310916E-4
56-57	0.0012339508597615026
58-59	0.004150178614884175
60-61	0.0028934714700997025
62-63	8.356708549407315E-4
64-65	1.2867891170288378E-4
66-67	1.8528023394519907E-6
68-69	3.1572976132997183E-4
70-71	0.012924116140653131
72-73	0.0041000384285938395
74-75	0.0028116227154966504
76-77	3.8657781716748015E-6
78-79	0.0
80-81	7.285050212602378E-5
82-83	9.941069340946867E-6
84-85	2.6096964314790175E-5
86-87	0.005418655280719792
88-89	0.0
90-91	8.495400362318465E-5
92-93	1.3216310664360652E-4
94-95	2.763914384817929E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	18563.0
20-21	35679.0
22-23	36620.0
24-25	39323.0
26-27	44948.0
28-29	57836.0
30-31	86761.0
32-33	99070.0
34-35	119567.0
36-37	153361.0
38-39	187324.0
40-41	193082.0
42-43	221211.0
44-45	261130.0
46-47	251548.0
48-49	224616.0
50-51	198501.0
52-53	190533.0
54-55	198890.0
56-57	208586.0
58-59	214682.0
60-61	214431.0
62-63	203052.0
64-65	209935.0
66-67	221186.0
68-69	229303.0
70-71	231143.0
72-73	217845.0
74-75	217577.0
76-77	227300.0
78-79	240527.0
80-81	247262.0
82-83	242174.0
84-85	240658.0
86-87	250659.0
88-89	271919.0
90-91	294195.0
92-93	308706.0
94-95	360697.0
96-97	1017474.0
98-99	2010382.0
100-101	2.0212079E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.93106273118312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.05594924062198	72.14517501087276
2	7.947975242924931	13.02372116427946
3	2.1173398010119238	5.204277001798161
4	0.8186493088143898	2.6829123150124583
5	0.40125905765463304	1.6437790512078583
6	0.2181515389355047	1.0724032452857384
7	0.1327277683778035	0.7612168981992232
8	0.08311047273456632	0.5447463484187229
9	0.05028609914844686	0.37079941894541035
>10	0.16974025967885373	2.138157123955322
>50	0.003621601978309543	0.19967142404559826
>100	0.0011180700611061315	0.14741699527754637
>500	5.5867714628242804E-5	0.030031920677519926
>1k	1.5670342928232514E-5	0.03569208202419207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2562327958975376E-6	0.0
2	0.0	3.581856075487291E-5	0.0	3.2562327958975376E-6	0.0
3	0.0	3.581856075487291E-5	0.0	3.2562327958975376E-6	0.0
4	0.0	1.1396814785641382E-4	0.0	3.2562327958975376E-6	0.0
5	0.0	1.1722438065231135E-4	0.0	3.2562327958975376E-6	0.0
6	3.907479355077045E-5	1.2373684624410641E-4	0.0	3.2562327958975376E-6	3.2562327958975376E-6
7	3.907479355077045E-5	1.2699307904000395E-4	0.0	3.2562327958975376E-6	3.2562327958975376E-6
8	4.558725914256552E-5	1.3676177742769657E-4	0.0	6.512465591795075E-6	3.2562327958975376E-6
9	4.558725914256552E-5	2.2468006291693007E-4	0.0	5.5355957530258135E-5	6.512465591795075E-6
10-11	5.047160833641183E-5	2.2468006291693007E-4	0.0	6.349653952000197E-5	6.512465591795075E-6
12-13	5.535595753025814E-5	2.2468006291693007E-4	0.0	9.605886747897736E-5	6.512465591795075E-6
14-15	6.512465591795074E-5	2.800360204471882E-4	0.0	1.0419944946872119E-4	1.628116397948769E-5
16-17	8.791828548923351E-5	2.898047188348808E-4	0.0	1.253649626420552E-4	1.628116397948769E-5
18-19	9.443075108102859E-5	3.3864821077334386E-4	0.0	1.416461266215429E-4	1.628116397948769E-5
20-21	9.605886747897736E-5	3.907479355077045E-4	0.0	1.725803381825695E-4	1.628116397948769E-5
22-23	1.1234003145846504E-4	4.884349193846306E-4	0.0	1.9374585135590347E-4	2.442174596923153E-5
24-25	1.1885249705026012E-4	5.617001572923253E-4	0.0	2.1328324813128872E-4	3.581856075487292E-5
26-27	1.2048061344820888E-4	5.828656704656592E-4	0.0	2.523580416820591E-4	3.907479355077045E-5
28-29	1.2048061344820888E-4	5.893781360574543E-4	0.0	4.233102634666799E-4	3.907479355077045E-5
30-31	1.2373684624410641E-4	6.561309083733537E-4	0.0	9.443075108102859E-4	4.0702909948719216E-5
32-33	1.2699307904000395E-4	6.626433739651489E-4	0.0	0.00219470090443494	4.884349193846306E-5
34-35	1.3024931183590148E-4	7.424210774646385E-4	0.0	0.003803279905608324	5.5355957530258135E-5
36-37	1.416461266215429E-4	8.270831301579745E-4	0.0	0.006308951042051479	5.5355957530258135E-5
38-39	1.4653047581538917E-4	8.563892253210523E-4	0.0	0.0105127475815552	5.8612190326155675E-5
40-41	1.4653047581538917E-4	9.231419976369519E-4	0.0	0.01515287931570919	5.8612190326155675E-5
42-43	1.514148250092355E-4	9.443075108102858E-4	0.0	0.019760448721904206	6.024030672410444E-5
44-45	1.562991742030818E-4	0.0010371101454933656	0.0	0.024457564529986403	6.186842312205321E-5
46-47	1.6281163979487686E-4	0.001113631616196958	0.0	0.029665908887024514	6.349653952000199E-5
48-49	1.6932410538667193E-4	0.0011250284309825991	0.0	0.034903559339225704	6.838088871384829E-5
50-51	1.9374585135590347E-4	0.00129598065276722	0.0	0.040375658552731515	6.838088871384829E-5
52-53	2.0514266614154485E-4	0.0013057493511549126	0.0	0.04566703684606502	8.466205269333597E-5
54-55	2.1165513173333994E-4	0.00130900558395081	0.0	0.05073210696008364	1.0419944946872119E-4
56-57	2.2305194651898132E-4	0.0013448241447056829	0.0	0.05543085088456378	1.1396814785641382E-4
58-59	2.3119252850872517E-4	0.0013692458906749145	0.0	0.06007749508430957	1.2048061344820888E-4
60-61	2.523580416820591E-4	0.0013969238694400436	0.0	0.06485438859589125	1.2373684624410641E-4
62-63	2.865484860389833E-4	0.0014278580810010702	0.0	0.06956452933515703	1.302493118359015E-4
64-65	2.8817660243693206E-4	0.0015792729060103057	0.0	0.07365110149400844	1.3350554463179904E-4
66-67	3.077139992123173E-4	0.0015922978371938957	0.0	0.0779363038534096	1.3513366102974782E-4
68-69	3.125983484061636E-4	0.0016590506095097953	0.0	0.08221824998001487	1.3838989382564533E-4
70-71	3.1911081399795866E-4	0.0016981254030605658	0.0	0.08601013307083755	1.4327424301949164E-4
72-73	3.3864821077334386E-4	0.001709522217846207	0.0	0.08896190810031868	1.6932410538667196E-4
74-75	3.435325599671902E-4	0.001751853244192875	0.0	0.09106055013727463	1.725803381825695E-4
76-77	3.467887927630877E-4	0.001821862249304672	0.0	0.09328618525327059	1.7420845458051827E-4
78-79	3.598137239466779E-4	0.0018462839952739037	0.0	0.09464891867835372	1.7583657097846702E-4
80-81	3.630699567425754E-4	0.001849540228069801	0.0	0.09516340346010553	1.8072092017231334E-4
82-83	3.679543059364217E-4	0.001857680810059545	0.0	0.09555415139561324	1.9211773495795472E-4
84-85	3.72838655130268E-4	0.0018690776248451865	0.0	0.09568602882384708	1.9211773495795472E-4
86-87	3.760948879261656E-4	0.0018967556036103156	0.0	0.09572347550099991	1.9537396775385225E-4
88	3.8749170271180694E-4	0.0019993269366810878	0.0	0.0957299879665917	1.9863020054974978E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	37240	0.0	49.07157	1
GTATCAA	65970	0.0	35.63017	1
TCAACGC	79695	0.0	28.942677	4
ATCAACG	80185	0.0	28.771084	3
CAACGCA	81870	0.0	28.11642	5
TATCAAC	84940	0.0	27.30102	2
AACGCAG	84840	0.0	27.224218	6
ACGCAGA	97260	0.0	23.672873	7
CGCAGAG	99085	0.0	23.253971	8
GTACATG	87975	0.0	19.913668	1
TACATGG	87250	0.0	19.565668	2
GCAGAGT	118550	0.0	19.166355	9
ACATGGG	89210	0.0	18.576277	3
GAGTACT	65700	0.0	18.57407	12-13
AGAGTAC	105160	0.0	18.26492	10-11
CAGAGTA	114850	0.0	17.59736	10-11
CATGGGG	60545	0.0	16.631348	4
GTACTTT	74960	0.0	16.225277	14-15
AGTACTT	72225	0.0	16.064507	12-13
GTGGTAT	17865	0.0	13.876012	1
>>END_MODULE