FastQCFastQC Report
Fri 27 May 2016
SRR2138896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54717425
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA695250.049.5786931
GTATCAA1256700.036.609651
TCAACGC1556250.028.9983024
ATCAACG1567800.028.795323
CAACGCA1586850.028.3994735
TATCAAC1637000.027.7845332
AACGCAG1646200.027.446486
ACGCAGA1884700.023.9057357
CGCAGAG1910350.023.5912328
GCAGAGT2236800.019.8274979
GTACATG1579150.019.4834921
TACATGG1579950.019.0028362
AGAGTAC2014000.018.53756310-11
ACATGGG1605400.018.267273
GAGTACT1316900.018.22462712-13
CAGAGTA2173500.017.8169210-11
AGTACTT1446050.016.33579412-13
CATGGGG1083550.016.214114
GTACTTT1501900.016.00139414-15
GTGGTAT316650.014.5881251
Produced by FastQC (version 0.11.5)