##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54717425
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.594092960332105	31.0	31.0	34.0	28.0	34.0
2	31.633889807497336	33.0	31.0	34.0	28.0	34.0
3	31.728524121886217	33.0	31.0	34.0	28.0	34.0
4	35.252355789768984	37.0	35.0	37.0	32.0	37.0
5	35.0724982215446	37.0	35.0	37.0	32.0	37.0
6	35.11974956789359	37.0	35.0	37.0	32.0	37.0
7	34.99660221583892	37.0	35.0	37.0	32.0	37.0
8	35.021544124198094	37.0	35.0	37.0	32.0	37.0
9	36.540786778617594	39.0	37.0	39.0	32.0	39.0
10-11	36.489291281525034	39.0	37.0	39.0	32.0	39.0
12-13	36.45771326044674	39.0	37.0	39.0	32.0	39.0
14-15	37.65013838827394	40.0	37.0	41.0	32.0	41.0
16-17	37.67144153804752	40.0	37.0	41.0	32.0	41.0
18-19	37.727931285143626	40.0	37.0	41.0	32.0	41.0
20-21	37.755503357664146	40.0	37.0	41.0	32.0	41.0
22-23	37.71996963021974	40.0	37.0	41.0	32.0	41.0
24-25	37.71357966753517	40.0	37.0	41.0	31.0	41.0
26-27	37.645046186228164	40.0	37.0	41.0	31.0	41.0
28-29	37.590896209325095	40.0	37.0	41.0	31.0	41.0
30-31	37.49962064143258	40.0	37.0	41.0	31.0	41.0
32-33	37.42374528618356	40.0	36.0	41.0	31.0	41.0
34-35	37.33544632342692	40.0	36.0	41.0	30.0	41.0
36-37	37.300596583789	40.0	36.0	41.0	30.0	41.0
38-39	37.24488048603396	40.0	36.0	41.0	30.0	41.0
40-41	37.226235256808266	40.0	36.0	41.0	30.0	41.0
42-43	37.190763337784674	40.0	36.0	41.0	30.0	41.0
44-45	37.11789296452032	40.0	36.0	41.0	30.0	41.0
46-47	37.04323366774531	39.5	35.5	41.0	30.0	41.0
48-49	36.98432712004963	39.0	35.0	41.0	30.0	41.0
50-51	36.91015150812359	39.0	35.0	41.0	30.0	41.0
52-53	37.12248591901225	39.0	36.0	41.0	30.0	41.0
54-55	37.11205821778965	39.0	35.0	41.0	30.0	41.0
56-57	37.027572348259696	39.0	35.0	41.0	30.0	41.0
58-59	36.891982919094225	39.0	35.0	41.0	30.0	41.0
60-61	36.76560213615193	39.0	35.0	41.0	30.0	41.0
62-63	36.60263317483597	38.5	35.0	41.0	30.0	41.0
64-65	36.39977113202719	38.0	35.0	40.0	30.0	41.0
66-67	36.170556907603284	37.5	34.5	40.0	29.5	41.0
68-69	35.913293214416235	37.0	34.0	40.0	29.0	41.0
70-71	35.66019992358683	36.5	34.0	39.5	29.0	41.0
72-73	35.382597890912194	36.0	34.0	39.0	29.0	41.0
74-75	35.11294656845364	36.0	34.0	39.0	29.0	40.5
76-77	34.818887402498504	35.0	34.0	37.5	29.0	40.0
78-79	34.54621686862572	35.0	34.0	37.0	29.0	39.0
80-81	34.288060156815035	35.0	34.0	37.0	29.0	39.0
82-83	34.03183158109455	35.0	33.0	36.0	29.0	38.5
84-85	33.822795521386176	35.0	33.0	36.0	29.0	37.0
86-87	33.60395016455351	35.0	33.0	36.0	29.0	37.0
88-89	33.46529944187262	35.0	33.0	35.0	29.0	36.5
90-91	33.36910560810436	35.0	33.0	35.0	29.0	36.0
92-93	33.300226279454584	35.0	33.0	35.0	29.0	36.0
94-95	33.35661206303236	35.0	33.0	35.0	29.0	36.0
96-97	33.54174703030516	35.0	33.5	35.0	30.0	36.0
98-99	33.63867894049688	35.0	34.0	35.0	30.0	35.5
100	32.84296504600573	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	27.0
15	71.0
16	164.0
17	805.0
18	5912.0
19	30141.0
20	93129.0
21	202828.0
22	335334.0
23	457528.0
24	566962.0
25	668724.0
26	761127.0
27	852944.0
28	959007.0
29	1089147.0
30	1248069.0
31	1461886.0
32	1756716.0
33	2159021.0
34	2777235.0
35	3710771.0
36	5472374.0
37	9415573.0
38	1.3097615E7
39	7260311.0
40	333996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94199653949359	21.2094264304287	14.427109828359065	24.421467201718645
2	14.374716484258213	22.74533129608382	40.04548973406612	22.834462485591853
3	16.943015867431626	27.99747064120799	31.481792134772423	23.57772135658796
4	12.228139712848511	19.7666005503151	39.69509475813952	28.310164978696868
5	12.395073615067153	38.82488591736893	36.16842732173753	12.61161314582638
6	27.679255920001992	40.10054909861437	19.568845058669908	12.651349922713727
7	24.51613052976481	33.74625906529045	24.75977299002875	16.977837414915992
8	23.888116634708382	36.59999532139461	22.140301141360407	17.37158690253661
9	25.40810621107449	16.475409857037864	21.58723377518134	36.529250156706304
10-11	23.18483950295255	27.14906024654108	30.062460641344853	19.60363960916152
12-13	24.51425283416117	24.938382238528394	29.772813228878892	20.774551698431544
14-15	21.838064245424274	26.043153263648843	27.344918547011865	24.773863943915018
16-17	21.23130838057739	29.099537755059757	27.873763283128373	21.795390581234482
18-19	21.323044104104884	28.289230599126007	29.539528207771156	20.84819708899795
20-21	22.213073824569086	27.44101311697664	29.320618746556722	21.025294311897554
22-23	21.893051183745303	27.40862807291351	29.19407290056002	21.50424784278116
24-25	21.648020062985932	27.584483930540472	29.2068573279574	21.560638678516195
26-27	21.64354289711755	27.699355390247522	29.10911641113923	21.5479853014957
28-29	21.636028677664665	27.60526714326653	29.192206806200428	21.566497372868376
30-31	21.849826023710854	27.553206799591358	29.068026245660732	21.528940931037056
32-33	21.72010163685002	27.703045352977938	28.99370041440633	21.583152595765707
34-35	21.794714257152393	27.701033931945453	28.945211981951648	21.559039828950507
36-37	21.83925863107823	27.662534410644597	28.855490235811114	21.642716722466066
38-39	21.736958783189422	27.71974941777865	28.920166718249305	21.62312508078262
40-41	21.884826481968002	27.628645684663784	28.817538770073774	21.66898906329444
42-43	21.831019457935565	27.752188089227065	28.620423920050293	21.79636853278708
44-45	21.892846032776177	27.831488054614784	28.434964986028767	21.840700926580272
46-47	22.035594055615086	27.853219495001774	28.10891273697128	22.002273712411863
48-49	21.96369389024795	27.94570911881269	28.08106421860565	22.00953277233371
50-51	21.95155260029846	28.05308304439757	27.96692887921724	22.028435476086734
52-53	22.104646205502867	27.971366744356324	27.919690593348705	22.004296456792105
54-55	22.057560981770393	27.913681280146836	27.97338583651079	22.055371901571977
56-57	22.070173627760187	28.012830918161786	27.91609163263781	22.00090382144022
58-59	22.049520951448027	28.021825057761713	27.92341947436175	22.005234516428512
60-61	22.10748325454128	28.01384265698623	27.901170512864876	21.977503575607614
62-63	22.05255683879367	28.1164052341831	27.84623382746426	21.984804099558964
64-65	22.04770371003936	28.033588696250355	27.829073247129354	22.08963434658093
66-67	22.105893208573864	28.061295968747935	27.776525157866743	22.056285664811455
68-69	22.06816798907118	28.021618691905744	27.82277173250541	22.08744158651767
70-71	22.091709617642604	27.823187568394392	27.884670323107645	22.20043249085536
72-73	22.11236419555662	27.878512438814056	27.805986563766673	22.203136801862648
74-75	21.964111068653853	27.997977363674032	27.826403161529157	22.211508406142958
76-77	22.103775200435212	27.952207412148734	27.780408801578282	22.16360858583777
78-79	22.056268374456288	27.95962542980114	27.760482523533053	22.22362367220952
80-81	22.089808617304886	27.958363859784825	27.72029700714926	22.23153051576103
82-83	22.074500883042685	27.938467235426273	27.792827832518757	22.194204049012285
84-85	22.101212337493102	27.94080960059327	27.8079992402147	22.149978821698934
86-87	22.028825973372427	27.965854790728823	27.835205134562464	22.17011410133629
88-89	22.079742921091647	27.95537161064804	27.795325000586786	22.169560467673534
90-91	22.086615212678375	27.979781005291542	27.81718790325256	22.11641587877752
92-93	22.12511170195588	27.962913113524383	27.778689490704835	22.13328569381491
94-95	21.961959728328896	27.984155862379723	27.860911446353185	22.192972962938196
96-97	22.013395775859262	28.129249002542974	27.918638936464234	21.93871628513353
98-99	23.073427221526718	30.08973847042529	28.112512429722553	18.72432187832544
100	22.140351064562225	31.081006641818327	21.845547504177283	24.933094789442162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10978.0
1	11205.0
2	17481.5
3	23689.5
4	28669.0
5	46828.5
6	63694.0
7	75066.5
8	82215.0
9	78031.5
10	65476.0
11	52074.5
12	53385.5
13	73282.5
14	125680.0
15	202771.5
16	267208.0
17	303433.5
18	312466.5
19	299409.5
20	275090.5
21	253513.0
22	247540.5
23	271508.0
24	327214.0
25	412476.0
26	530273.5
27	659062.5
28	786004.0
29	921177.0
30	1053179.5
31	1178689.0
32	1311914.5
33	1441946.0
34	1566670.5
35	1680989.0
36	1795051.5
37	1902409.0
38	1981113.0
39	2046391.0
40	2114473.5
41	2174972.0
42	2218000.0
43	2257862.0
44	2292346.0
45	2318479.0
46	2314417.0
47	2295440.0
48	2272454.0
49	2220840.5
50	2157140.5
51	2079164.0
52	1978565.5
53	1854951.5
54	1724117.0
55	1592907.0
56	1458545.5
57	1316938.0
58	1167474.0
59	1017558.0
60	857510.0
61	700731.5
62	560208.0
63	436653.0
64	332698.5
65	252507.5
66	191610.5
67	147431.5
68	114785.0
69	86988.5
70	65239.5
71	49244.0
72	36637.5
73	26633.5
74	19363.0
75	14234.5
76	10260.0
77	7263.5
78	5338.5
79	3991.5
80	2967.0
81	2225.5
82	1755.5
83	1205.0
84	800.5
85	612.5
86	487.5
87	375.0
88	239.5
89	146.5
90	103.5
91	82.5
92	59.0
93	30.0
94	17.0
95	16.5
96	16.0
97	14.0
98	10.0
99	14.0
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012900826382089434
3	0.0
4	0.12760834414265657
5	0.03616946521149341
6	0.004866822588965032
7	0.005161061581388379
8	4.843064161005384E-4
9	7.65752408853304E-4
10-11	0.004841236589623872
12-13	8.132692647726021E-5
14-15	6.213742697138982E-5
16-17	4.0663463238630105E-4
18-19	2.284464226889332E-5
20-21	8.798628094493278E-4
22-23	0.030278223855432725
24-25	0.0010516830422060118
26-27	0.004540404884017584
28-29	5.785121192540652E-4
30-31	0.0026726572294792634
32-33	0.003483240852795402
34-35	0.019916000563314957
36-37	0.004575066473477295
38-39	0.0016824835288569056
40-41	0.0010544998499180255
42-43	9.74678648259302E-4
44-45	0.030877784387092256
46-47	0.004030320891511637
48-49	0.005900830262872003
50-51	0.019640729066943242
52-53	0.00445786665881143
54-55	0.001030380611973516
56-57	0.0012740389863810486
58-59	0.004171928441371813
60-61	0.003002856012036828
62-63	8.996580677459109E-4
64-65	1.4317910773100191E-4
66-67	4.2169836719130195E-6
68-69	3.2667007602400083E-4
70-71	0.013372577755399177
72-73	0.004147108245719298
74-75	0.0029623350591872575
76-77	3.3148688874881236E-6
78-79	0.0
80-81	5.863962123134995E-5
82-83	4.5598164126715936E-6
84-85	2.304054851988726E-5
86-87	0.005654279556058013
88-89	0.0
90-91	7.035766557797123E-5
92-93	1.3174093432918684E-4
94-95	3.065436574817522E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	33957.0
20-21	66251.0
22-23	68152.0
24-25	73284.0
26-27	86056.0
28-29	114360.0
30-31	169564.0
32-33	194221.0
34-35	236748.0
36-37	301172.0
38-39	365479.0
40-41	376154.0
42-43	432492.0
44-45	512140.0
46-47	496413.0
48-49	443967.0
50-51	395177.0
52-53	371738.0
54-55	390315.0
56-57	407740.0
58-59	420955.0
60-61	420723.0
62-63	397784.0
64-65	407875.0
66-67	434573.0
68-69	450004.0
70-71	451143.0
72-73	422519.0
74-75	418196.0
76-77	437301.0
78-79	462427.0
80-81	480140.0
82-83	463068.0
84-85	457589.0
86-87	476147.0
88-89	515449.0
90-91	555053.0
92-93	579747.0
94-95	662166.0
96-97	1778642.0
98-99	3471971.0
100-101	3.5018573E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.6932444486615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29160290841006	69.56551048895415
2	8.2587055971561	13.163260879673802
3	2.2274654579552333	5.325418477253284
4	0.9018955963954522	2.8749994492285653
5	0.45554198181773503	1.8151809256814238
6	0.2548342406831049	1.2185140461988633
7	0.1677563653065135	0.9358334319733658
8	0.10748113434093623	0.6852416250121327
9	0.06934166401196205	0.4973455962524022
>10	0.25740126898082943	3.191053518109458
>50	0.005409557999357777	0.2965527861759495
>100	0.002472926119394029	0.3266523211321498
>500	4.671618248155328E-5	0.027436795045216362
>1k	4.4584640854085516E-5	0.07699965930920266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.8275713815114655E-6	0.0	0.0	0.0
2	0.0	2.0103285196626122E-5	0.0	3.655142763022931E-6	0.0
3	0.0	2.375842795964905E-5	0.0	3.655142763022931E-6	0.0
4	0.0	6.39649983529013E-5	0.0	7.310285526045862E-6	0.0
5	0.0	6.762014111592422E-5	0.0	7.310285526045862E-6	0.0
6	1.8275713815114656E-5	6.944771249743569E-5	0.0	7.310285526045862E-6	1.8275713815114655E-6
7	1.8275713815114656E-5	7.127528387894715E-5	0.0	7.310285526045862E-6	1.8275713815114655E-6
8	1.8275713815114656E-5	7.675799802348155E-5	0.0	7.310285526045862E-6	1.8275713815114655E-6
9	2.0103285196626122E-5	1.4072299637638285E-4	0.0	2.5585999341160517E-5	1.8275713815114655E-6
10-11	2.2844642268893316E-5	1.4620571052091725E-4	0.0	2.924114210418345E-5	1.8275713815114655E-6
12-13	2.375842795964905E-5	1.4986085328394017E-4	0.0	3.198249917645064E-5	1.8275713815114655E-6
14-15	2.375842795964905E-5	1.9463635213097107E-4	0.0	3.7465213320985043E-5	6.396499835290129E-6
16-17	3.289628486720638E-5	2.037742090385284E-4	0.0	4.294792746551944E-5	1.2792999670580258E-5
18-19	3.289628486720638E-5	2.2204992285364305E-4	0.0	6.213742697138984E-5	1.4620571052091724E-5
20-21	3.7465213320985043E-5	2.49463493576315E-4	0.0	7.401664095121435E-5	1.553435674284746E-5
22-23	4.1120356084007975E-5	2.8418734982503286E-4	0.0	9.868885460161914E-5	2.1930856578137585E-5
24-25	4.203414177476371E-5	3.106871348569491E-4	0.0	1.33412710850337E-4	2.924114210418345E-5
26-27	4.203414177476371E-5	3.271352772905523E-4	0.0	1.8184335246039082E-4	3.106871348569492E-5
28-29	4.203414177476371E-5	3.307904200535752E-4	0.0	3.993243468602552E-4	3.289628486720638E-5
30-31	4.203414177476371E-5	3.8744513288043066E-4	0.0	0.0010471984016060696	3.289628486720638E-5
32-33	4.751685591929811E-5	3.956692040972323E-4	0.0	0.0024781867933295473	3.289628486720638E-5
34-35	5.29995700638325E-5	4.5597905968711063E-4	0.0	0.004239051819415844	3.289628486720638E-5
36-37	5.4827141445343965E-5	5.071510583694316E-4	0.0	0.007007822462405715	3.289628486720638E-5
38-39	5.5740927136099696E-5	5.318232720198364E-4	0.0	0.011480803418655025	3.289628486720638E-5
40-41	5.665471282685543E-5	5.839090563929132E-4	0.0	0.016310160794299075	3.289628486720638E-5
42-43	6.305121266214556E-5	6.122364128063409E-4	0.0	0.02073105596617531	3.289628486720638E-5
44-45	6.762014111592422E-5	6.789427682315094E-4	0.0	0.02562803348293528	3.381007055796211E-5
46-47	7.127528387894715E-5	7.282871955323191E-4	0.0	0.03113359226973857	3.472385624871784E-5
48-49	7.218906956970289E-5	7.355974810583649E-4	0.0	0.03660442720029314	3.8378999011740775E-5
50-51	8.315449785877168E-5	8.141830504633579E-4	0.0	0.042456310763892856	3.8378999011740775E-5
52-53	8.406828354952741E-5	8.224071216801595E-4	0.0	0.04781566384017523	4.112035608400797E-5
54-55	8.406828354952741E-5	8.333725499692283E-4	0.0	0.05267152100085119	4.477549884703091E-5
56-57	8.406828354952741E-5	8.4799312102132E-4	0.0	0.05662273032767898	4.568928453778664E-5
58-59	8.406828354952741E-5	8.525620494750986E-4	0.0	0.06101986707159557	4.751685591929811E-5
60-61	9.2292354766329E-5	8.808894058885263E-4	0.0	0.06536491803113907	5.117199868232103E-5
62-63	1.05999140127665E-4	9.06475405229687E-4	0.0	0.06950893613871632	5.29995700638325E-5
64-65	1.05999140127665E-4	0.001001509117068283	0.0	0.07331576732640471	5.29995700638325E-5
66-67	1.1056806858144365E-4	0.0010133883310481076	0.0	0.0770376164448528	5.29995700638325E-5
68-69	1.1330942565371085E-4	0.0010471984016060696	0.0	0.08094405027283355	5.3913355754588234E-5
70-71	1.1879213979824527E-4	0.0010700430438749632	0.0	0.08430860918619618	5.84822842083669E-5
72-73	1.3524028223184845E-4	0.001080094686473276	0.0	0.08704631111570034	6.579256973441276E-5
74-75	1.3615406792260418E-4	0.001104766900123681	0.0	0.08921837970262672	6.579256973441276E-5
76-77	1.398092106856271E-4	0.0011696456841673378	0.0	0.09129906972047752	6.67063554251685E-5
78-79	1.4620571052091725E-4	0.0011943178978177428	0.0	0.09259024890151538	6.762014111592422E-5
80-81	1.4620571052091725E-4	0.001199800611962277	0.0	0.09305719338949156	7.218906956970289E-5
82-83	1.507746389746959E-4	0.0012061971117975673	0.0	0.09337062188142077	7.858556940499301E-5
84-85	1.5534356742847455E-4	0.0012199038971589032	0.0	0.09345925909342409	7.858556940499301E-5
86-87	1.589987101914975E-4	0.001240920968046285	0.0	0.09349215537829128	7.858556940499301E-5
88	1.6265385295452043E-4	0.0013158513946882552	0.0	0.09349672430674506	7.858556940499301E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	69525	0.0	49.578693	1
GTATCAA	125670	0.0	36.60965	1
TCAACGC	155625	0.0	28.998302	4
ATCAACG	156780	0.0	28.79532	3
CAACGCA	158685	0.0	28.399473	5
TATCAAC	163700	0.0	27.784533	2
AACGCAG	164620	0.0	27.44648	6
ACGCAGA	188470	0.0	23.905735	7
CGCAGAG	191035	0.0	23.591232	8
GCAGAGT	223680	0.0	19.827497	9
GTACATG	157915	0.0	19.483492	1
TACATGG	157995	0.0	19.002836	2
AGAGTAC	201400	0.0	18.537563	10-11
ACATGGG	160540	0.0	18.26727	3
GAGTACT	131690	0.0	18.224627	12-13
CAGAGTA	217350	0.0	17.81692	10-11
AGTACTT	144605	0.0	16.335794	12-13
CATGGGG	108355	0.0	16.21411	4
GTACTTT	150190	0.0	16.001394	14-15
GTGGTAT	31665	0.0	14.588125	1
>>END_MODULE