FastQCFastQC Report
Fri 27 May 2016
SRR2138895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6771500
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102850.051.602641
GTATCAA186350.036.903921
CTTATAC78900.031.9395141
TCAACGC226700.029.7925174
ATCAACG228050.029.6907833
TATCAAC233100.029.229552
CAACGCA233350.028.9214655
AACGCAG238350.028.3643866
TATACAC108950.025.9337833
ACGCAGA268950.025.1372037
GTGGTAT45700.024.8792611
CGCAGAG272200.024.837078
TTATACA111400.024.1828442
TGGTATC43400.023.949432
GCAGAGT313850.021.4734559
GAGTACT188750.020.5605312-13
TACACAT137400.020.4375655
ACATCTC128000.020.3119078
TACATGG221450.020.153892
GTACATG226550.020.149631
Produced by FastQC (version 0.11.5)