##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138895_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6771500
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80890216347929	33.0	33.0	33.0	33.0	33.0
2	32.80514538876172	33.0	33.0	33.0	33.0	33.0
3	32.80590135125157	33.0	33.0	33.0	33.0	33.0
4	36.75046016392232	37.0	37.0	37.0	37.0	37.0
5	36.761687366167024	37.0	37.0	37.0	37.0	37.0
6	36.763597873440155	37.0	37.0	37.0	37.0	37.0
7	36.75057727239164	37.0	37.0	37.0	37.0	37.0
8	36.75026951192498	37.0	37.0	37.0	37.0	37.0
9	36.738232887838734	37.0	37.0	37.0	37.0	37.0
10-11	36.712749907701394	37.0	37.0	37.0	37.0	37.0
12-13	36.697901794284874	37.0	37.0	37.0	37.0	37.0
14-15	38.95629853060622	40.0	40.0	40.0	37.0	40.0
16-17	38.904222624233924	40.0	40.0	40.0	37.0	40.0
18-19	38.942914642250614	40.0	40.0	40.0	37.0	40.0
20-21	38.975348771557904	40.0	40.0	40.0	37.0	40.0
22-23	39.00596638088585	40.0	40.0	40.0	37.0	40.0
24-25	38.996446440433964	40.0	40.0	40.0	37.0	40.0
26-27	38.90959437432201	40.0	40.0	40.0	37.0	40.0
28-29	38.83195322104767	40.0	38.5	40.0	37.0	40.0
30-31	38.7659826242196	40.0	37.0	40.0	37.0	40.0
32-33	38.72595234371297	40.0	37.0	40.0	37.0	40.0
34-35	38.69151286640143	40.0	37.0	40.0	37.0	40.0
36-37	38.65745389310573	40.0	37.0	40.0	37.0	40.0
38-39	38.6026846865379	40.0	37.0	40.0	37.0	40.0
40-41	38.569394413629766	40.0	37.0	40.0	37.0	40.0
42-43	38.51495480564152	40.0	37.0	40.0	37.0	40.0
44-45	38.427519694256574	40.0	37.0	40.0	37.0	40.0
46-47	38.339479721744254	40.0	37.0	40.0	37.0	40.0
48-49	38.29299771211035	40.0	37.0	40.0	37.0	40.0
50-51	38.22641361358919	40.0	37.0	40.0	37.0	40.0
52-53	38.16783493632373	40.0	37.0	40.0	37.0	40.0
54-55	38.09798810818907	40.0	37.0	40.0	37.0	40.0
56-57	37.988300444362864	40.0	37.0	40.0	35.0	40.0
58-59	37.85668224031466	40.0	37.0	40.0	33.0	40.0
60-61	37.687101927977146	40.0	37.0	40.0	33.0	40.0
62-63	37.49837400486335	37.0	37.0	40.0	33.0	40.0
64-65	37.2811272449586	37.0	37.0	40.0	33.0	40.0
66-67	37.04902769489289	37.0	37.0	40.0	33.0	40.0
68-69	36.784206907073894	37.0	37.0	40.0	33.0	40.0
70-71	36.530467040289395	37.0	37.0	40.0	33.0	40.0
72-73	36.28932518830241	37.0	37.0	37.0	33.0	40.0
74-75	36.03159987319542	37.0	37.0	37.0	33.0	40.0
76-77	34.383756840695085	35.0	33.0	37.0	30.0	37.0
78-79	35.640691219825854	37.0	35.0	37.0	33.0	37.0
80-81	35.771077065995755	37.0	37.0	37.0	33.0	37.0
82-83	35.68286805198585	37.0	37.0	37.0	33.0	37.0
84-85	35.57374991774582	37.0	37.0	37.0	33.0	37.0
86-87	35.48122081222475	37.0	35.0	37.0	33.0	37.0
88-89	35.38623580745425	37.0	33.0	37.0	33.0	37.0
90-91	35.324166884764	37.0	33.0	37.0	33.0	37.0
92-93	35.25584555406172	37.0	33.0	37.0	33.0	37.0
94-95	35.214946901354	37.0	33.0	37.0	33.0	37.0
96-97	35.17940059058809	37.0	33.0	37.0	33.0	37.0
98-99	35.14486767023696	37.0	33.0	37.0	33.0	37.0
100	35.0771133264407	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	7.0
20	22.0
21	119.0
22	432.0
23	1342.0
24	3067.0
25	6412.0
26	11638.0
27	19662.0
28	30023.0
29	42943.0
30	59167.0
31	80895.0
32	109114.0
33	149478.0
34	215180.0
35	329987.0
36	638574.0
37	1925392.0
38	3018880.0
39	129164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7145216612336	20.125871705197284	14.52894174966898	23.630664883900135
2	14.541715880297094	20.766542351485594	42.00016008274683	22.69158168547048
3	18.018475686458558	26.217375367976402	30.809449140765686	24.954699804799354
4	12.389199370090278	18.30817662640274	38.58619474443076	30.71642925907622
5	13.289774474424002	37.57684880963177	35.49485272115605	13.63852399478818
6	29.622234253041203	37.777519833868375	18.74690061194731	13.853345301143117
7	26.19514526990257	32.955524323344065	22.675865417686687	18.173464989066677
8	24.445429291210115	36.47772819578058	21.11209054302452	17.964751969984786
9	25.50253643986361	16.850700265923393	20.980952666462773	36.665810627750226
10-11	24.11836551121358	26.72973689210196	28.698820696951984	20.453076899732476
12-13	25.131979620468137	24.28476703832238	28.7716901720446	21.811563169164884
14-15	22.33005980949568	25.715661227202247	26.48481872554087	25.469460237761204
16-17	21.732348814885917	29.02907036845603	27.069851583844052	22.168729232813998
18-19	21.736941593443106	27.741172561470872	29.040087129882597	21.481798715203425
20-21	22.64507953732625	26.878064229295635	28.974940929696686	21.50191530368143
22-23	22.344660573081192	27.042528600927753	28.83674606759628	21.776064758394778
24-25	22.149538474493205	27.28274803510497	28.91518884819351	21.65252464220832
26-27	22.064027319900063	27.44636547758083	28.869653918365536	21.61995328415357
28-29	21.806928843799582	27.275308180799403	28.94204758540852	21.97571538999249
30-31	22.379306959327476	27.204970222586113	28.863929872291124	21.551792945795288
32-33	22.190386934839594	27.375138156049328	28.859421037337402	21.575053871773683
34-35	22.097087589418496	27.475000293681212	28.910424850397305	21.51748726650299
36-37	22.149719897133647	27.279251518446095	28.76700549905363	21.804023085366627
38-39	21.86307871742873	27.251209484338624	28.96569762816481	21.920014170067834
40-41	22.06187038730929	27.140518864682324	28.90918621768992	21.888424530318467
42-43	22.166724371990625	27.171517767661935	28.754418412480902	21.907339447866537
44-45	22.048587110671203	27.487456523029962	28.436565932712867	22.02739043358597
46-47	22.396486320036722	27.281269826904058	27.92444800814292	22.397795844916303
48-49	22.237579692572456	27.422044041146794	27.95702272460594	22.383353541674815
50-51	22.139441331933103	27.656957224725492	27.950962741657648	22.252638701683754
52-53	22.259126207497708	27.644965279847057	28.007502636093545	22.088405876561694
54-55	22.228645825161518	27.569563030274114	27.775684903544022	22.426106241020342
56-57	22.40505548247321	27.818750902314864	27.516251356903926	22.259942258307998
58-59	22.129369645675283	27.980483762494305	27.812545537941848	22.077601053888564
60-61	22.40484861808921	28.122628332511653	27.424029298917812	22.048493750481327
62-63	22.119497658995474	28.297458202638254	27.439253271316417	22.143790867049855
64-65	22.193984056394708	28.154034223672124	27.431045594238988	22.220936125694177
66-67	22.321683004098926	28.058121231933246	27.307052360057725	22.3131434039101
68-69	22.33189875467331	27.881510882711027	27.414958738514024	22.371631624101642
70-71	22.359890674389415	27.62714662012525	27.51691375026032	22.49604895522502
72-73	22.415195947256876	27.62769386145361	27.44642934568625	22.51068084560326
74-75	22.298101366735445	27.775106655027905	27.391490784508605	22.53530119372805
76-77	22.44095054113697	27.69028139086831	27.34804358309021	22.520724484904513
78-79	22.445811121129346	27.71522068739915	27.271696633732162	22.567271557739343
80-81	22.429524003568073	27.681278525076085	27.301070284328887	22.588127187026956
82-83	22.505349593073333	27.63517111601295	27.364239963326554	22.495239327587164
84-85	22.575680041385397	27.58848812107912	27.362124460320107	22.473707377215373
86-87	22.41502361566969	27.578091816216876	27.44956418140978	22.557320386703648
88-89	22.521959921571437	27.57464696858971	27.38654834307086	22.516844766767992
90-91	22.5847474589146	27.488160893208097	27.443278633179403	22.483813014697905
92-93	22.615306513806914	27.41057497166525	27.445057555028455	22.529060959499382
94-95	22.495392567521485	27.509266411103518	27.487944149353773	22.507396872021225
96-97	22.54689579860169	27.62270367283113	27.588830222024065	22.241570306543117
98-99	23.668328032061098	29.48007438583953	27.79446335703552	19.057134225063855
100	22.590012518464047	30.474034947302485	21.621960700997356	25.31399183323611
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1326.0
1	1203.5
2	1625.5
3	2362.5
4	3367.0
5	5597.5
6	7521.5
7	8876.5
8	9756.0
9	9838.5
10	9369.0
11	8851.5
12	9910.0
13	13273.5
14	20934.5
15	31279.5
16	39820.0
17	45235.0
18	46861.5
19	44689.5
20	40056.5
21	34647.0
22	31196.0
23	31622.0
24	35799.5
25	43697.0
26	55027.5
27	68298.0
28	81271.5
29	95225.5
30	109133.5
31	122269.5
32	137525.5
33	152846.0
34	168095.5
35	182230.0
36	196926.0
37	210823.5
38	220624.0
39	229818.5
40	240687.0
41	249796.0
42	256374.5
43	263812.0
44	269812.0
45	275306.5
46	280292.5
47	285109.5
48	295349.5
49	295808.0
50	285333.5
51	276835.0
52	264112.0
53	246055.5
54	227691.5
55	209865.5
56	191927.5
57	173857.5
58	155390.5
59	137029.0
60	116504.5
61	95726.5
62	76456.0
63	59073.0
64	44546.5
65	33176.0
66	24850.0
67	19157.5
68	14625.0
69	10448.0
70	7311.5
71	5209.5
72	3663.0
73	2453.5
74	1653.0
75	1082.0
76	696.5
77	436.5
78	274.5
79	193.5
80	138.0
81	98.5
82	70.0
83	50.0
84	35.0
85	24.0
86	18.5
87	14.5
88	10.0
89	6.5
90	3.5
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.953555342243225E-5
2	2.953555342243225E-5
3	8.860666026729677E-5
4	0.014856383371483423
5	0.0108986192128775
6	2.953555342243225E-5
7	1.4767776711216126E-5
8	1.4767776711216126E-5
9	1.4767776711216126E-5
10-11	7.383888355608063E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008995918821951193
26-27	0.05206991198352676
28-29	0.08105062182663829
30-31	0.06924039483746931
32-33	0.03294189348046341
34-35	0.004178277122371478
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.7217074904111843E-4
56-57	6.136510883263538E-4
58-59	8.149966556612236E-6
60-61	1.6455626005747295E-5
62-63	1.6616418616769556E-5
64-65	8.393667716350772E-6
66-67	0.0
68-69	0.0
70-71	5.2041614209442345E-5
72-73	0.015562816062227442
74-75	0.026098167478978022
76-77	0.001303507998941123
78-79	0.0
80-81	1.8232343320129776E-5
82-83	0.01288270764657812
84-85	0.05061594529864479
86-87	0.03870247026448069
88-89	0.009572138732959331
90-91	4.822935566545418E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	358.0
22-23	819.0
24-25	1874.0
26-27	2326.0
28-29	5401.0
30-31	13094.0
32-33	16949.0
34-35	23874.0
36-37	34726.0
38-39	41565.0
40-41	44412.0
42-43	52952.0
44-45	64191.0
46-47	62847.0
48-49	55145.0
50-51	47329.0
52-53	45244.0
54-55	51205.0
56-57	57778.0
58-59	57596.0
60-61	59313.0
62-63	60526.0
64-65	62917.0
66-67	69500.0
68-69	60599.0
70-71	56397.0
72-73	53361.0
74-75	55285.0
76-77	55489.0
78-79	58620.0
80-81	59487.0
82-83	57318.0
84-85	57312.0
86-87	60117.0
88-89	64779.0
90-91	67842.0
92-93	70997.0
94-95	77141.0
96-97	215927.0
98-99	400874.0
100-101	4367948.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.98278912381926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.66827919000438	78.86579809003722
2	6.819665182325578	11.863869968985565
3	1.4788617934342623	3.859065705646968
4	0.48489566532144884	1.687103096148385
5	0.21231384458113364	0.9233825185634043
6	0.11877593636032001	0.6198877335248335
7	0.061995505121447	0.3774779364022437
8	0.04051092104067199	0.2819002321673971
9	0.02518926237273754	0.1971929067437146
>10	0.08766679004721897	1.1360167221522721
>50	0.0014554271612772628	0.08347853210770241
>100	3.402400899934448E-4	0.06062197029529875
>500	2.5121070332395184E-5	0.01662861680861683
>1k	2.512106922439821E-5	0.02757597041641298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4767776711216126E-5	0.0	0.0	0.0
3	0.0	1.4767776711216126E-5	0.0	0.0	0.0
4	0.0	4.430333013364838E-5	0.0	2.953555342243225E-5	0.0
5	0.0	4.430333013364838E-5	0.0	2.953555342243225E-5	0.0
6	0.0	4.430333013364838E-5	0.0	2.953555342243225E-5	0.0
7	0.0	4.430333013364838E-5	0.0	2.953555342243225E-5	0.0
8	0.0	4.430333013364838E-5	0.0	2.953555342243225E-5	0.0
9	0.0	1.6244554382337738E-4	0.0	7.383888355608063E-5	0.0
10-11	0.0	1.6244554382337738E-4	0.0	8.860666026729675E-5	0.0
12-13	0.0	1.6244554382337738E-4	0.0	8.860666026729675E-5	0.0
14-15	0.0	2.8797164586871445E-4	0.0	1.0337443697851288E-4	0.0
16-17	0.0	2.953555342243225E-4	0.0	1.1075832533412094E-4	0.0
18-19	0.0	3.1012331093553864E-4	0.0	1.2552610204533707E-4	1.4767776711216126E-5
20-21	0.0	3.2489108764675477E-4	0.0	1.993649856014177E-4	1.4767776711216126E-5
22-23	0.0	3.54426641069187E-4	0.0	4.72568854758916E-4	1.4767776711216126E-5
24-25	0.0	4.208816362696596E-4	0.0	0.0012700287971645868	1.4767776711216126E-5
26-27	0.0	4.72568854758916E-4	0.0	0.004164513032562947	1.4767776711216126E-5
28-29	0.0	4.72568854758916E-4	0.0	0.012914420733958503	1.4767776711216126E-5
30-31	0.0	5.390238499593886E-4	0.0	0.01453149228383667	1.4767776711216126E-5
32-33	0.0	5.464077383149967E-4	0.0	0.01838588200546408	1.4767776711216126E-5
34-35	0.0	6.793177287159418E-4	0.0	0.023325703315365872	2.953555342243225E-5
36-37	0.0	8.122277191168869E-4	0.0	0.03142582884146792	2.953555342243225E-5
38-39	0.0	8.26995495828103E-4	0.0	0.04899948312781511	2.953555342243225E-5
40-41	0.0	8.712988259617514E-4	0.0	0.06680942184154176	2.953555342243225E-5
42-43	0.0	8.860666026729675E-4	0.0	0.07823229712766742	3.6919441778040314E-5
44-45	0.0	9.525215978734401E-4	0.0	0.09353171380048733	4.430333013364838E-5
46-47	0.0	0.0010042088163626966	0.0	0.10877205936646238	4.430333013364838E-5
48-49	0.0	0.0010042088163626966	0.0	0.12255777892638263	5.90711068448645E-5
50-51	0.0	0.001166654360186074	0.0	0.13582662630141035	5.90711068448645E-5
52-53	0.0	0.00118142213689729	0.0	0.1685815550468877	5.90711068448645E-5
54-55	0.0	0.00118142213689729	0.0	0.20725097836520712	5.90711068448645E-5
56-57	0.0	0.00118142213689729	0.0	0.2467843166211327	5.90711068448645E-5
58-59	0.0	0.0012035738019641144	0.0	0.2704570626892121	5.90711068448645E-5
60-61	0.0	0.0012331093553865466	0.0	0.28707819537768586	5.90711068448645E-5
62-63	0.0	0.001284796573875803	0.0	0.2999335450047995	5.90711068448645E-5
64-65	0.0	0.0014324743409879642	0.0	0.30552314848999484	5.90711068448645E-5
66-67	0.0	0.0014472421176991803	0.0	0.3116000886066602	5.90711068448645E-5
68-69	0.0	0.0014620098944103964	0.0	0.31741859263087946	5.90711068448645E-5
70-71	0.0	0.0014767776711216126	0.0	0.32235841394078124	5.90711068448645E-5
72-73	0.0	0.0014841615594772205	0.0	0.3259543675699623	5.90711068448645E-5
74-75	0.0	0.001535848777966477	0.0	0.3287750129218046	5.90711068448645E-5
76-77	0.0	0.0015801521081001255	0.0	0.331839326589382	5.90711068448645E-5
78-79	0.0	0.0016096876615225577	0.0	0.333892047552241	5.90711068448645E-5
80-81	0.0	0.0016096876615225577	0.0	0.3345935169460238	5.90711068448645E-5
82-83	0.0	0.0016096876615225577	0.0	0.33519161190282803	5.90711068448645E-5
84-85	0.0	0.0016096876615225577	0.0	0.33539097688842945	5.90711068448645E-5
86-87	0.0	0.0016244554382337738	0.0	0.3354869674370523	5.90711068448645E-5
88	0.0	0.0017425976519235028	0.0	0.33549435132540795	5.90711068448645E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10285	0.0	51.60264	1
GTATCAA	18635	0.0	36.90392	1
CTTATAC	7890	0.0	31.939514	1
TCAACGC	22670	0.0	29.792517	4
ATCAACG	22805	0.0	29.690783	3
TATCAAC	23310	0.0	29.22955	2
CAACGCA	23335	0.0	28.921465	5
AACGCAG	23835	0.0	28.364386	6
TATACAC	10895	0.0	25.933783	3
ACGCAGA	26895	0.0	25.137203	7
GTGGTAT	4570	0.0	24.879261	1
CGCAGAG	27220	0.0	24.83707	8
TTATACA	11140	0.0	24.182844	2
TGGTATC	4340	0.0	23.94943	2
GCAGAGT	31385	0.0	21.473455	9
GAGTACT	18875	0.0	20.56053	12-13
TACACAT	13740	0.0	20.437565	5
ACATCTC	12800	0.0	20.311907	8
TACATGG	22145	0.0	20.15389	2
GTACATG	22655	0.0	20.14963	1
>>END_MODULE