FastQCFastQC Report
Fri 27 May 2016
SRR2138894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662070
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97600.053.0789641
GTATCAA182250.037.459131
TCAACGC222300.030.0630844
ATCAACG223050.029.9240443
TATCAAC228800.029.4864942
CAACGCA229350.029.1710645
AACGCAG235100.028.4728286
ACGCAGA266950.025.0915647
CGCAGAG270500.024.7622668
GTGGTAT44650.022.6551691
TGGTATC43100.022.2915082
GCAGAGT312900.021.2580669
GAGTACT188300.020.0892612-13
CAGAGTA305300.019.2232310-11
GTACATG207500.018.9694921
AGAGTAC284000.018.96648210-11
TACATGG206550.018.4215152
GTACTTT208150.018.16357814-15
ACATGGG207750.017.7650453
AGTACTT203250.017.44577412-13
Produced by FastQC (version 0.11.5)