##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6662070
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80943370453928	33.0	33.0	33.0	33.0	33.0
2	32.80164273266417	33.0	33.0	33.0	33.0	33.0
3	32.804154414468776	33.0	33.0	33.0	33.0	33.0
4	36.74988539598053	37.0	37.0	37.0	37.0	37.0
5	36.76206869636614	37.0	37.0	37.0	37.0	37.0
6	36.76689152170422	37.0	37.0	37.0	37.0	37.0
7	36.75381360448029	37.0	37.0	37.0	37.0	37.0
8	36.755100741961584	37.0	37.0	37.0	37.0	37.0
9	36.7421571673669	37.0	37.0	37.0	37.0	37.0
10-11	36.71661142858001	37.0	37.0	37.0	37.0	37.0
12-13	36.7015282787482	37.0	37.0	37.0	37.0	37.0
14-15	38.9688989308128	40.0	40.0	40.0	37.0	40.0
16-17	38.92387426130317	40.0	40.0	40.0	37.0	40.0
18-19	38.959731810083056	40.0	40.0	40.0	37.0	40.0
20-21	38.9889463978182	40.0	40.0	40.0	37.0	40.0
22-23	39.02293545730699	40.0	40.0	40.0	37.0	40.0
24-25	39.01419113548391	40.0	40.0	40.0	37.0	40.0
26-27	38.932452385655345	40.0	40.0	40.0	37.0	40.0
28-29	38.863793887487546	40.0	40.0	40.0	37.0	40.0
30-31	38.79761554455865	40.0	37.0	40.0	37.0	40.0
32-33	38.76000914842991	40.0	37.0	40.0	37.0	40.0
34-35	38.73750422286704	40.0	37.0	40.0	37.0	40.0
36-37	38.70626795243465	40.0	37.0	40.0	37.0	40.0
38-39	38.666532798786676	40.0	37.0	40.0	37.0	40.0
40-41	38.62176799435541	40.0	37.0	40.0	37.0	40.0
42-43	38.55647935602983	40.0	37.0	40.0	37.0	40.0
44-45	38.47052180030012	40.0	37.0	40.0	37.0	40.0
46-47	38.385479810419	40.0	37.0	40.0	37.0	40.0
48-49	38.33797203959064	40.0	37.0	40.0	37.0	40.0
50-51	38.299077316799	40.0	37.0	40.0	37.0	40.0
52-53	38.23936198601302	40.0	37.0	40.0	37.0	40.0
54-55	38.16753894065489	40.0	37.0	40.0	37.0	40.0
56-57	38.06565951536169	40.0	37.0	40.0	37.0	40.0
58-59	37.93453289851816	40.0	37.0	40.0	35.0	40.0
60-61	37.76992966846382	40.0	37.0	40.0	33.0	40.0
62-63	37.580929903006734	38.5	37.0	40.0	33.0	40.0
64-65	37.35080073847318	37.0	37.0	40.0	33.0	40.0
66-67	37.11112875402239	37.0	37.0	40.0	33.0	40.0
68-69	36.83952238464982	37.0	37.0	40.0	33.0	40.0
70-71	36.57775916306377	37.0	37.0	40.0	33.0	40.0
72-73	36.33485554652695	37.0	37.0	37.0	33.0	40.0
74-75	36.07135724324792	37.0	37.0	37.0	33.0	40.0
76-77	34.414025781572846	35.0	33.0	37.0	30.0	37.0
78-79	35.66725415137101	37.0	35.0	37.0	33.0	37.0
80-81	35.798048263936195	37.0	37.0	37.0	33.0	37.0
82-83	35.7105430660509	37.0	37.0	37.0	33.0	37.0
84-85	35.60438272626722	37.0	37.0	37.0	33.0	37.0
86-87	35.51279568379332	37.0	35.0	37.0	33.0	37.0
88-89	35.41840235750272	37.0	33.0	37.0	33.0	37.0
90-91	35.358511045059444	37.0	33.0	37.0	33.0	37.0
92-93	35.288160639358594	37.0	33.0	37.0	33.0	37.0
94-95	35.24669314818398	37.0	33.0	37.0	33.0	37.0
96-97	35.209591597715324	37.0	33.0	37.0	33.0	37.0
98-99	35.17011352845244	37.0	33.0	37.0	33.0	37.0
100	35.10059190749384	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	25.0
21	93.0
22	370.0
23	1094.0
24	2791.0
25	5753.0
26	10563.0
27	17536.0
28	27322.0
29	39641.0
30	55958.0
31	76297.0
32	104076.0
33	142643.0
34	206137.0
35	316530.0
36	610468.0
37	1871695.0
38	3037629.0
39	135439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99151314831576	20.03111645479558	14.396351284210462	23.58101911267819
2	14.656480643403627	21.143983776814114	41.598467143095164	22.601068436687097
3	17.926745869300646	26.244898731144744	30.843891116091882	24.98446428346273
4	12.465784868433596	18.208177223159748	39.13925815634842	30.186779752058236
5	13.301086873140688	37.69073872275855	35.52517390105358	13.483000503047183
6	29.63734995279245	37.972672157452564	18.901737748177368	13.488240141577618
7	26.217902243596956	33.03009424998537	23.175184289567657	17.57681921685002
8	24.443829020109366	36.25686911125221	21.31559710420335	17.98370476443508
9	25.84046700199788	16.753471518612084	21.187633873555818	36.21842760583422
10-11	24.047608325940736	26.74274662379711	29.02583581379361	20.183809236468544
12-13	25.09931597836708	24.449150189055356	29.000048033118837	21.451485799458727
14-15	22.291292346072616	25.748401022505018	26.90286202336511	25.057444608057256
16-17	21.60119152155411	28.8199463530104	27.504041536639512	22.074820588795973
18-19	21.640285977181268	27.85920141937866	29.40696360140317	21.093549002036905
20-21	22.57759799673698	26.99862097040633	29.295785261060193	21.127995771796495
22-23	22.264725410837052	26.889469141979518	29.149026016965347	21.696779430218086
24-25	21.966736397856845	27.034606996809053	29.233861113578303	21.7647954917558
26-27	21.866109361915864	27.227114584206003	29.140784692201954	21.765991361676182
28-29	21.820331749623872	27.35051976533921	29.13520620943839	21.69394227559853
30-31	22.16316854547432	27.139509230576426	29.032058719811467	21.665263504137794
32-33	21.91111330308113	27.31522182912558	28.93939620174832	21.83426866604497
34-35	22.031818404893503	27.365019959947773	28.92411983364177	21.679041801516956
36-37	22.051625873367826	27.29845838319586	28.863661405785702	21.78625433765061
38-39	21.894383783180523	27.4659057775643	28.88934161719184	21.75036882206334
40-41	22.198788097286286	27.308492870377904	28.700379917365936	21.792339114969877
42-43	22.090150856721248	27.422214829930507	28.47008767431849	22.017546639029757
44-45	22.196112307638185	27.541153799548923	28.254689582657438	22.00804431015545
46-47	22.263657422109144	27.556401217019438	27.944808819314826	22.235132541556588
48-49	22.197751964607743	27.709519267934564	27.899914997632617	22.192813769825076
50-51	22.241742270978087	27.827319591776956	27.720435455082427	22.210502682162534
52-53	22.423204039011335	27.71082138729496	27.57064822887731	22.2953263448164
54-55	22.387682792368665	27.60378648152552	27.662148583748227	22.346382142357584
56-57	22.36874325666023	27.715445943871337	27.6224676249609	22.293343174507534
58-59	22.3219038753046	27.751900122305194	27.64070753354742	22.285488468842786
60-61	22.393076313633863	27.709953134861486	27.652370449448764	22.24460010205589
62-63	22.361030890791824	27.75568014892955	27.600107761923365	22.28318119835526
64-65	22.334164055070254	27.731172841721026	27.560987653308484	22.373675449900237
66-67	22.40969730181511	27.774155344141167	27.40641554373141	22.409731810312312
68-69	22.36417501158722	27.754433866377497	27.47064933588964	22.41074178614564
70-71	22.36694292950503	27.519931581380124	27.52973259094575	22.583392898169098
72-73	22.40806662782914	27.532929750458806	27.47396171597336	22.58504190573869
74-75	22.31305434615655	27.65412763338438	27.458738405729953	22.57407961472911
76-77	22.421782589573823	27.595359161448783	27.361784645131255	22.621073603846146
78-79	22.443673302031648	27.59820074655656	27.33207157516534	22.626054376246447
80-81	22.44855122773866	27.57937955069863	27.31394436226053	22.658124859302184
82-83	22.495153769556804	27.532314382076407	27.419804307370356	22.55272754099644
84-85	22.585113345674994	27.517466372586135	27.35724698387523	22.540173297863646
86-87	22.435935853447802	27.50303786779522	27.420065563391454	22.640960715365523
88-89	22.521586062046307	27.44015191268326	27.41950441733915	22.618757607931283
90-91	22.56542899270295	27.403104887034708	27.45587183217334	22.575594288089
92-93	22.555999756316545	27.36800895636568	27.469437201075802	22.606554086241974
94-95	22.473292893249713	27.399002913060304	27.49040913685221	22.637295056837768
96-97	22.54272746045948	27.525148673060585	27.548216537933932	22.383907328546
98-99	23.661142936621754	29.403542871042067	27.78914696457644	19.14616722775974
100	22.562166470083422	30.396585291601863	21.542998255260194	25.498249983054517
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1193.0
1	1104.0
2	1608.5
3	2394.0
4	3400.5
5	5739.0
6	7744.0
7	9110.5
8	10119.0
9	10308.0
10	9732.0
11	9013.5
12	9950.0
13	13028.0
14	20133.0
15	30035.0
16	38313.5
17	43403.0
18	44698.0
19	42487.0
20	38233.0
21	33359.5
22	30280.5
23	30812.5
24	34838.0
25	42381.5
26	53268.0
27	65861.5
28	78527.0
29	92849.5
30	107188.5
31	120696.5
32	136394.5
33	152460.5
34	168266.5
35	183502.0
36	199944.0
37	214961.0
38	226177.0
39	235979.5
40	246422.0
41	255669.0
42	262830.5
43	270143.5
44	274449.5
45	277838.0
46	279702.0
47	280799.0
48	281343.0
49	277753.0
50	271431.5
51	262782.0
52	251119.0
53	236669.5
54	221991.5
55	206416.0
56	189297.5
57	170846.0
58	152120.0
59	133599.5
60	112918.0
61	92460.5
62	73480.0
63	56901.0
64	43153.0
65	32160.0
66	24295.5
67	18538.0
68	14097.5
69	10111.0
70	7185.0
71	5104.0
72	3386.0
73	2255.0
74	1495.5
75	1015.5
76	687.0
77	433.0
78	273.5
79	185.0
80	126.5
81	86.0
82	55.0
83	33.0
84	25.0
85	22.0
86	18.0
87	16.5
88	13.5
89	8.0
90	6.0
91	7.5
92	6.5
93	5.0
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.0020699272148145E-5
4	0.013644407819191334
5	0.010011903207261407
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.00882855111389514
26-27	0.05174905186815504
28-29	0.08154635448953101
30-31	0.06955397002958771
32-33	0.03234120468499666
34-35	0.004378134071017418
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.76053593850183E-4
56-57	8.574676112222062E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.7611873925400506E-5
72-73	0.01546025939433144
74-75	0.026587074961714252
76-77	0.0012983255141754903
78-79	0.0
80-81	9.279327523422646E-6
82-83	0.0133075878120139
84-85	0.05067784464279665
86-87	0.039381682621272444
88-89	0.01022549152829691
90-91	3.9400354189483985E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	79.0
20-21	377.0
22-23	764.0
24-25	2141.0
26-27	2412.0
28-29	5704.0
30-31	14008.0
32-33	18473.0
34-35	25285.0
36-37	36703.0
38-39	43154.0
40-41	46047.0
42-43	55097.0
44-45	65823.0
46-47	64013.0
48-49	55927.0
50-51	49387.0
52-53	47975.0
54-55	50997.0
56-57	53266.0
58-59	53597.0
60-61	52222.0
62-63	52326.0
64-65	55921.0
66-67	58738.0
68-69	59008.0
70-71	57751.0
72-73	55994.0
74-75	57410.0
76-77	58050.0
78-79	59815.0
80-81	60941.0
82-83	59315.0
84-85	59980.0
86-87	62866.0
88-89	66529.0
90-91	69985.0
92-93	72153.0
94-95	79113.0
96-97	214735.0
98-99	394316.0
100-101	4263673.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.87919192528844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.34742608940952	80.2753798919232
2	6.294564931037947	11.063225593217474
3	1.3798814588287096	3.6378860266366546
4	0.48916428079355273	1.7194944685940923
5	0.20555003771774555	0.903178560742402
6	0.09540499473436982	0.5030468305735691
7	0.06129539112960827	0.37706126088501274
8	0.03288648011226877	0.23120298380265988
9	0.022566066493434445	0.17847789195378116
>10	0.06989676684794946	0.9236787465388338
>50	8.860199711362377E-4	0.05340496015693788
>100	4.047888262942386E-4	0.07921506877465724
>500	4.84627366888367E-5	0.029854651390727325
>1k	2.4231360780267944E-5	0.024893064810008664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.0020699272148145E-5	0.0	1.5010349636074072E-5	0.0
5	0.0	3.0020699272148145E-5	0.0	1.5010349636074072E-5	0.0
6	0.0	3.0020699272148145E-5	0.0	1.5010349636074072E-5	1.5010349636074072E-5
7	0.0	4.503104890822222E-5	0.0	1.5010349636074072E-5	1.5010349636074072E-5
8	0.0	4.503104890822222E-5	0.0	1.5010349636074072E-5	1.5010349636074072E-5
9	0.0	1.5010349636074074E-4	0.0	3.0020699272148145E-5	1.5010349636074072E-5
10-11	0.0	1.5010349636074074E-4	0.0	4.5031048908222215E-5	1.5010349636074072E-5
12-13	0.0	1.5010349636074074E-4	0.0	7.505174818037037E-5	1.5010349636074072E-5
14-15	0.0	2.1765006972307405E-4	0.0	1.050724474525185E-4	3.0020699272148145E-5
16-17	0.0	2.251552445411111E-4	0.0	1.5010349636074074E-4	4.503104890822222E-5
18-19	0.0	3.002069927214814E-4	0.0	2.6268111863129626E-4	5.253622372625925E-5
20-21	0.0	3.752587409018518E-4	0.0	3.227225171755926E-4	6.004139854429629E-5
22-23	0.0	4.202897898100741E-4	0.0	5.628881113527778E-4	6.754657336233333E-5
24-25	0.0	4.4280531426418515E-4	0.0	0.0015535711873336665	8.25569229984074E-5
26-27	0.0	4.5781566390025924E-4	0.0	0.004495599716004185	9.006209781644444E-5
28-29	0.0	4.653208387182963E-4	0.0	0.013531830196920776	9.006209781644444E-5
30-31	0.0	5.328674120806296E-4	0.0	0.014680121944080443	9.006209781644444E-5
32-33	0.0	5.478777617167036E-4	0.0	0.017021736487308	9.006209781644444E-5
34-35	0.0	6.22929509897074E-4	0.0	0.019716094246983295	9.006209781644444E-5
36-37	0.0	6.904760832594074E-4	0.0	0.02421169396298748	9.006209781644444E-5
38-39	0.0	7.204967825315556E-4	0.0	0.03495159912759848	9.006209781644444E-5
40-41	0.0	7.655278314397777E-4	0.0	0.046854806389005216	9.006209781644444E-5
42-43	0.0	7.805381810758518E-4	0.0	0.05528311770966081	9.756727263448149E-5
44-45	0.0	8.630951040742592E-4	0.0	0.06537757783992063	1.1257762227055555E-4
46-47	0.0	9.306416774365926E-4	0.0	0.07580977083699211	1.275879719066296E-4
48-49	0.0	9.381468522546297E-4	0.0	0.08569408607234688	2.1014489490503704E-4
50-51	0.0	0.001028208950071074	0.0	0.09543580298615895	2.1014489490503704E-4
52-53	0.0	0.001035714124889111	0.0	0.11721582030810243	2.4016559417718518E-4
54-55	0.0	0.0010657348241612592	0.0	0.14346892182159598	2.701862934493333E-4
56-57	0.0	0.0011257762227055554	0.0	0.17111798585124444	2.7769146826737035E-4
58-59	0.0	0.0011332813975235925	0.0	0.19592258862485684	2.851966430854074E-4
60-61	0.0	0.0011482917471596667	0.0	0.20773573378844715	2.851966430854074E-4
62-63	0.0	0.0011933227960678888	0.0	0.22117750188755148	2.851966430854074E-4
64-65	0.0	0.0012908900687023702	0.0	0.2281122834194177	2.851966430854074E-4
66-67	0.0	0.0012908900687023702	0.0	0.2339062783789423	2.851966430854074E-4
68-69	0.0	0.0013584366420647037	0.0	0.24008303725418678	2.851966430854074E-4
70-71	0.0	0.0013809521665188148	0.0	0.24561435109508006	3.302276919936296E-4
72-73	0.0	0.0013809521665188148	0.0	0.25004240423772195	4.05279440174E-4
74-75	0.0	0.001418478040609	0.0	0.25343474325547466	4.05279440174E-4
76-77	0.0	0.0015235504880615185	0.0	0.25704473234295044	4.05279440174E-4
78-79	0.0	0.0015610763621517036	0.0	0.25937884171135994	4.05279440174E-4
80-81	0.0	0.0015760867117877776	0.0	0.2601518747176178	4.05279440174E-4
82-83	0.0	0.0015760867117877776	0.0	0.2607597938778788	4.277949646281111E-4
84-85	0.0	0.0015910970614238518	0.0	0.2610825163950544	4.353001394461481E-4
86-87	0.0	0.0015986022362418886	0.0	0.26126264059068727	4.353001394461481E-4
88	1.5010349636074072E-5	0.0018162523059649628	0.0	0.2612851561151414	4.353001394461481E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9760	0.0	53.078964	1
GTATCAA	18225	0.0	37.45913	1
TCAACGC	22230	0.0	30.063084	4
ATCAACG	22305	0.0	29.924044	3
TATCAAC	22880	0.0	29.486494	2
CAACGCA	22935	0.0	29.171064	5
AACGCAG	23510	0.0	28.472828	6
ACGCAGA	26695	0.0	25.091564	7
CGCAGAG	27050	0.0	24.762266	8
GTGGTAT	4465	0.0	22.655169	1
TGGTATC	4310	0.0	22.291508	2
GCAGAGT	31290	0.0	21.258066	9
GAGTACT	18830	0.0	20.08926	12-13
CAGAGTA	30530	0.0	19.22323	10-11
GTACATG	20750	0.0	18.969492	1
AGAGTAC	28400	0.0	18.966482	10-11
TACATGG	20655	0.0	18.421515	2
GTACTTT	20815	0.0	18.163578	14-15
ACATGGG	20775	0.0	17.765045	3
AGTACTT	20325	0.0	17.445774	12-13
>>END_MODULE