FastQCFastQC Report
Fri 27 May 2016
SRR2138893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7770897
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118950.052.3170931
GTATCAA223800.038.769921
CTTATAC68350.037.0774541
TCAACGC275950.030.9965324
ATCAACG278950.030.6483553
TATCAAC283650.030.245282
CAACGCA283550.030.1616555
AACGCAG292650.029.2049486
TATACAC103750.028.31343
GTGGTAT51800.027.4529571
TGGTATC50500.026.9828432
ACGCAGA332150.025.7318337
CGCAGAG338050.025.3329498
TTATACA108650.024.4578742
ACACATC117450.022.1128086
GCAGAGT386000.022.0760789
ACATCTC119350.021.6185578
TACACAT136200.021.0970335
GTACATG272200.020.4762611
TACATGG266750.020.4649472
Produced by FastQC (version 0.11.5)