##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7770897
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.808428808154325	33.0	33.0	33.0	33.0	33.0
2	32.80456040017002	33.0	33.0	33.0	33.0	33.0
3	32.805292619372	33.0	33.0	33.0	33.0	33.0
4	36.750878309157876	37.0	37.0	37.0	37.0	37.0
5	36.76501387162897	37.0	37.0	37.0	37.0	37.0
6	36.76709291089561	37.0	37.0	37.0	37.0	37.0
7	36.7542369947768	37.0	37.0	37.0	37.0	37.0
8	36.752620193009896	37.0	37.0	37.0	37.0	37.0
9	36.74154759740092	37.0	37.0	37.0	37.0	37.0
10-11	36.71623237317391	37.0	37.0	37.0	37.0	37.0
12-13	36.702138826444354	37.0	37.0	37.0	37.0	37.0
14-15	38.961906263845734	40.0	40.0	40.0	37.0	40.0
16-17	38.91260230318328	40.0	40.0	40.0	37.0	40.0
18-19	38.95354976394617	40.0	40.0	40.0	37.0	40.0
20-21	38.98521957993604	40.0	40.0	40.0	37.0	40.0
22-23	39.01740385298889	40.0	40.0	40.0	37.0	40.0
24-25	39.00660324591148	40.0	40.0	40.0	37.0	40.0
26-27	38.92384367064662	40.0	40.0	40.0	37.0	40.0
28-29	38.8507516929336	40.0	40.0	40.0	37.0	40.0
30-31	38.77852172878963	40.0	37.0	40.0	37.0	40.0
32-33	38.74117742158144	40.0	37.0	40.0	37.0	40.0
34-35	38.714851044886636	40.0	37.0	40.0	37.0	40.0
36-37	38.685208937040336	40.0	37.0	40.0	37.0	40.0
38-39	38.63662957737017	40.0	37.0	40.0	37.0	40.0
40-41	38.603032975002094	40.0	37.0	40.0	37.0	40.0
42-43	38.54375261356799	40.0	37.0	40.0	37.0	40.0
44-45	38.4551412670194	40.0	37.0	40.0	37.0	40.0
46-47	38.36812494927411	40.0	37.0	40.0	37.0	40.0
48-49	38.322408111026164	40.0	37.0	40.0	37.0	40.0
50-51	38.27656991100358	40.0	37.0	40.0	37.0	40.0
52-53	38.22685726708939	40.0	37.0	40.0	37.0	40.0
54-55	38.15053997017916	40.0	37.0	40.0	37.0	40.0
56-57	38.049519037741305	40.0	37.0	40.0	37.0	40.0
58-59	37.9237648500755	40.0	37.0	40.0	33.0	40.0
60-61	37.76104848604831	40.0	37.0	40.0	33.0	40.0
62-63	37.578352593754246	38.5	37.0	40.0	33.0	40.0
64-65	37.36043789214811	37.0	37.0	40.0	33.0	40.0
66-67	37.128373237119675	37.0	37.0	40.0	33.0	40.0
68-69	36.86519235447172	37.0	37.0	40.0	33.0	40.0
70-71	36.60872364786938	37.0	37.0	40.0	33.0	40.0
72-73	36.36632428207466	37.0	37.0	37.0	33.0	40.0
74-75	36.103078418424985	37.0	37.0	37.0	33.0	40.0
76-77	34.45383299805552	35.0	33.0	37.0	30.0	37.0
78-79	35.700087067166194	37.0	35.0	37.0	33.0	37.0
80-81	35.821097996688906	37.0	37.0	37.0	33.0	37.0
82-83	35.72882692742566	37.0	37.0	37.0	33.0	37.0
84-85	35.61586362328806	37.0	37.0	37.0	33.0	37.0
86-87	35.51909004673037	37.0	37.0	37.0	33.0	37.0
88-89	35.42383606817808	37.0	33.0	37.0	33.0	37.0
90-91	35.3615595723683	37.0	33.0	37.0	33.0	37.0
92-93	35.29119142352656	37.0	33.0	37.0	33.0	37.0
94-95	35.24610714608419	37.0	33.0	37.0	33.0	37.0
96-97	35.208291549670946	37.0	33.0	37.0	33.0	37.0
98-99	35.16739235495815	37.0	33.0	37.0	33.0	37.0
100	35.09341125679464	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	21.0
21	94.0
22	437.0
23	1316.0
24	3222.0
25	6940.0
26	12763.0
27	21624.0
28	33112.0
29	47752.0
30	65926.0
31	90270.0
32	122711.0
33	169847.0
34	242698.0
35	372112.0
36	713212.0
37	2165295.0
38	3550288.0
39	151250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.591697843891126	20.30617572205628	14.673441174165609	23.428685259886986
2	14.422028756783162	21.022528029904397	42.15802886076086	22.39741435255158
3	17.844519739695198	26.513784135407914	31.035441242894958	24.60625488200193
4	12.196964617058363	18.53459842587179	39.005163736696666	30.263273220373186
5	13.080496063284993	37.88068736175295	35.54926302342441	13.489553551537654
6	29.16662001825529	38.18111345446993	18.936990156992174	13.715276370282606
7	25.91008476884972	33.3133485104744	22.88591651645878	17.890650204217092
8	24.323871491283438	36.531149492780564	21.299857661219807	17.84512135471619
9	25.580804892922913	16.958402614267055	21.120019992543977	36.340772500266056
10-11	23.874650506884855	27.027098673422127	28.8507813190678	20.24746950062522
12-13	25.015915151108036	24.595764169824925	28.89544797724124	21.4928727018258
14-15	22.195449251225437	25.95087800031322	26.755489102480706	25.098183645980637
16-17	21.437023293449904	29.240877854898862	27.344468727355416	21.977630124295818
18-19	21.45300600432614	28.001722323690558	29.450422518790305	21.094849153192996
20-21	22.456418813838138	27.062268433828653	29.326374482078705	21.154938270254508
22-23	22.087720265731008	27.195356972356592	29.16678276209129	21.55013999982111
24-25	21.795772823454634	27.430941902470813	29.373827549862824	21.39945772421173
26-27	21.791436961238407	27.573301979084512	29.282524214561377	21.352736845115697
28-29	21.568459085799134	27.442264460419953	29.471866525180758	21.517409928600156
30-31	22.002823941531684	27.37995344793943	29.225226694803702	21.391995915725182
32-33	21.81529300532408	27.366446228301207	29.247383398584088	21.57087736779063
34-35	21.650651304497757	27.648204275775456	29.413808781480643	21.28733563824614
36-37	21.914866021093758	27.457008458135178	29.075433213228862	21.552692307542205
38-39	21.628665027700876	27.48220584128454	29.235258300616152	21.653870830398432
40-41	21.828671488373764	27.394249780609126	29.1755904928295	21.60148823818761
42-43	21.975030255893753	27.393535769153132	28.951904825713992	21.679529149239126
44-45	21.854162260188424	27.70974680454008	28.69747756473078	21.738613370540723
46-47	22.144464722843978	27.520860652281094	28.195294695520474	22.139379929354458
48-49	22.01817550291796	27.64660266848097	28.19122011610479	22.14400171249628
50-51	21.91799948536306	27.854294637003246	28.18528194727728	22.04242393035641
52-53	22.05202908556987	27.90415995913518	28.16921468005395	21.874596275241
54-55	22.03638678727649	27.823005686569896	28.01938990490737	22.12121762124624
56-57	22.214656139129914	28.066156346921368	27.711527013275905	22.007660500672813
58-59	21.942531870293177	28.236829927090355	28.035054568333003	21.78558363428346
60-61	22.20056232050863	28.373957127024706	27.649883207983052	21.775597344483614
62-63	21.974130937530987	28.510433254540818	27.62111859588102	21.89431721204718
64-65	22.00335254234461	28.37556053106354	27.655036063166616	21.96605086342523
66-67	22.166286905426137	28.238608494068686	27.55749374148348	22.0376108590217
68-69	22.161263875807457	28.08981237352921	27.67185037730593	22.077073373357404
70-71	22.201364570585923	27.811179464715853	27.73756111489483	22.249894849803393
72-73	22.187490068195153	27.916227507820263	27.691453377545315	22.20482904643927
74-75	22.08105140787016	28.03095867446031	27.61961786804464	22.268372049624894
76-77	22.17356767016292	28.003687960529255	27.56815708798935	22.254587281318475
78-79	22.21388998986544	28.053906006983816	27.472133098867136	22.26007090428361
80-81	22.216169668686582	27.990321581462013	27.505774983500896	22.287733766350506
82-83	22.23017895035479	27.988922292386015	27.616048030609754	22.16485072664944
84-85	22.34501627577159	27.95169491374658	27.535618362065605	22.167670448416228
86-87	22.11693410523741	27.998376847910205	27.614328344844825	22.27036070200756
88-89	22.25285274245276	28.005175258709258	27.54120049949108	22.2007714993469
90-91	22.298721622583777	27.970399252010814	27.570020177695053	22.160858947710352
92-93	22.250092740401474	27.98059530795311	27.565399110199618	22.2039128414458
94-95	22.14115298047588	28.061045436131447	27.57910642929003	22.218695154102644
96-97	22.238693718677315	28.13460782737383	27.672244978550392	21.954453475398463
98-99	23.329406687275657	30.057516816237218	27.843481640025075	18.769594856462053
100	22.24376142986414	31.111931987166912	21.723287339665504	24.921019243303444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2902.0
1	2422.0
2	2635.5
3	3567.0
4	4765.5
5	7372.5
6	9525.0
7	11086.5
8	12384.0
9	12625.5
10	11984.5
11	11412.0
12	12777.0
13	16820.5
14	25854.0
15	38166.5
16	48613.0
17	55030.0
18	56113.5
19	52701.5
20	46935.0
21	40610.0
22	36661.0
23	37175.0
24	42469.0
25	51980.0
26	65601.5
27	81396.0
28	95920.0
29	111418.5
30	127798.0
31	144886.5
32	164154.0
33	182623.0
34	201684.0
35	219753.0
36	238420.0
37	256481.0
38	268154.0
39	276832.0
40	287875.0
41	297621.5
42	303451.0
43	309483.5
44	315043.5
45	321645.5
46	330200.5
47	338675.0
48	335913.5
49	326068.0
50	316900.0
51	301287.5
52	282638.5
53	263508.0
54	244482.5
55	226454.0
56	208033.5
57	187893.0
58	167252.0
59	146041.0
60	122417.0
61	99564.5
62	78864.5
63	60452.0
64	45480.0
65	33958.5
66	25320.5
67	19086.0
68	14505.0
69	10319.0
70	7111.5
71	5063.5
72	3447.0
73	2272.0
74	1536.5
75	1045.0
76	707.0
77	444.5
78	271.5
79	186.5
80	132.0
81	96.5
82	70.5
83	41.5
84	22.0
85	16.0
86	12.0
87	9.5
88	9.0
89	8.5
90	6.0
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	9.007969092885931E-5
4	0.014618646985026311
5	0.010809562911463117
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.009081087871849953
26-27	0.05244284110153915
28-29	0.0814356480604171
30-31	0.07000102654416229
32-33	0.03325635115863848
34-35	0.004334563511398153
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.7696245607514786E-4
56-57	7.465083655769781E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.2649471791668344E-5
72-73	0.013960691660826216
74-75	0.024047749124529658
76-77	0.0010092413905110505
78-79	0.0
80-81	1.5839771210344638E-5
82-83	0.013123144305375568
84-85	0.04838258614864591
86-87	0.03819500079133966
88-89	0.009875220479159568
90-91	5.848361673380029E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	98.0
20-21	408.0
22-23	924.0
24-25	2198.0
26-27	3386.0
28-29	6287.0
30-31	14864.0
32-33	19590.0
34-35	27266.0
36-37	39050.0
38-39	47503.0
40-41	50223.0
42-43	61447.0
44-45	74477.0
46-47	72314.0
48-49	63475.0
50-51	55988.0
52-53	54264.0
54-55	60752.0
56-57	65395.0
58-59	64759.0
60-61	66414.0
62-63	68201.0
64-65	70495.0
66-67	76345.0
68-69	66330.0
70-71	62123.0
72-73	59691.0
74-75	61206.0
76-77	61241.0
78-79	64651.0
80-81	65392.0
82-83	62413.0
84-85	63112.0
86-87	65674.0
88-89	70263.0
90-91	72624.0
92-93	75183.0
94-95	89396.0
96-97	245319.0
98-99	463228.0
100-101	5056928.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.3426043410172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0311978252969	77.73528092177459
2	7.247947352910902	12.516133011538177
3	1.6064626144981287	4.161184977367439
4	0.5639400640593704	1.947682152925044
5	0.22593760589043624	0.9754020655577307
6	0.11625686298472443	0.6022752193570745
7	0.06422027639700945	0.3881462140932426
8	0.0373549797267548	0.2580260987771127
9	0.02468980499687991	0.19186038576922249
>10	0.0803658015721504	1.0306080187623392
>50	0.0012352979359950803	0.07048703696122953
>100	3.0356969140457206E-4	0.047417414468158225
>500	6.59580892138013E-5	0.04256055789956419
>1k	2.198595012882282E-5	0.03293592474914484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.286852727555133E-5
2	0.0	2.573705455110266E-5	0.0	0.0	1.286852727555133E-5
3	0.0	2.573705455110266E-5	0.0	0.0	1.286852727555133E-5
4	0.0	3.860558182665399E-5	0.0	1.286852727555133E-5	1.286852727555133E-5
5	1.286852727555133E-5	3.860558182665399E-5	0.0	1.286852727555133E-5	1.286852727555133E-5
6	1.286852727555133E-5	3.860558182665399E-5	0.0	1.286852727555133E-5	1.286852727555133E-5
7	1.286852727555133E-5	6.434263637775664E-5	0.0	3.860558182665399E-5	1.286852727555133E-5
8	1.286852727555133E-5	7.721116365330798E-5	0.0	5.147410910220532E-5	2.573705455110266E-5
9	1.286852727555133E-5	1.4155380003106464E-4	0.0	6.434263637775664E-5	2.573705455110266E-5
10-11	5.147410910220532E-5	1.4155380003106464E-4	0.0	1.0294821820441064E-4	2.573705455110266E-5
12-13	7.721116365330798E-5	1.4155380003106464E-4	0.0	1.1581674547996197E-4	3.217131818887832E-5
14-15	7.721116365330798E-5	1.9302790913326993E-4	0.0	1.3511953639328895E-4	7.077690001553232E-5
16-17	7.721116365330798E-5	2.0589643640882128E-4	0.0	1.6729085458216727E-4	1.1581674547996197E-4
18-19	7.721116365330798E-5	2.2519922732214828E-4	0.0	1.994621727710456E-4	1.286852727555133E-4
20-21	7.721116365330798E-5	2.4450201823547526E-4	0.0	2.2519922732214828E-4	1.286852727555133E-4
22-23	9.007969092885931E-5	3.088446546132319E-4	0.0	2.509362818732509E-4	1.5442232730661595E-4
24-25	9.007969092885931E-5	3.345817091643346E-4	0.0	3.474502364398859E-4	1.9302790913326993E-4
26-27	9.007969092885931E-5	3.474502364398859E-4	0.0	4.246614000931939E-4	1.9302790913326993E-4
28-29	9.007969092885931E-5	3.474502364398859E-4	0.0	5.726494637620341E-4	1.994621727710456E-4
30-31	1.0938248184218631E-4	3.6031876371543723E-4	0.0	0.001228944354815152	2.1233070004659694E-4
32-33	1.286852727555133E-4	3.7318729099098855E-4	0.0	0.00256727119147249	2.4450201823547526E-4
34-35	1.415538000310646E-4	4.3109566373096954E-4	0.0	0.004426773382789658	2.8310760006212927E-4
36-37	1.5442232730661595E-4	4.890040364709505E-4	0.0	0.0072449808561353985	2.8310760006212927E-4
38-39	1.5442232730661595E-4	4.954383001087262E-4	0.0	0.011189184466091882	2.8310760006212927E-4
40-41	1.5442232730661595E-4	5.919522546753612E-4	0.0	0.015365021567008287	2.8310760006212927E-4
42-43	1.5442232730661595E-4	6.176893092264638E-4	0.0	0.01950225308609804	2.8310760006212927E-4
44-45	1.5442232730661595E-4	7.270717910686502E-4	0.0	0.02397406631435213	2.8310760006212927E-4
46-47	1.608565909443916E-4	8.107172183597338E-4	0.0	0.02956544141557918	2.8310760006212927E-4
48-49	1.6729085458216727E-4	8.171514819975095E-4	0.0	0.0353498444259395	2.8310760006212927E-4
50-51	1.6729085458216727E-4	9.715738093041253E-4	0.0	0.04106990479992207	2.8954186369990493E-4
52-53	1.7372511821994296E-4	9.844423365796768E-4	0.0	0.04656476594658249	2.959761273376806E-4
54-55	1.8659364549549428E-4	0.0010037451274930037	0.0	0.05184086212955853	3.0241039097545625E-4
56-57	1.9302790913326993E-4	0.0010037451274930037	0.0	0.058024189485460946	3.088446546132319E-4
58-59	1.9302790913326993E-4	0.0010166136547685551	0.0	0.06389867218675013	3.217131818887832E-4
60-61	1.9302790913326993E-4	0.0010294821820441063	0.0	0.0698825373698815	3.3458170916433454E-4
62-63	1.9302790913326993E-4	0.0010616535002329847	0.0	0.07575058580753291	3.7318729099098855E-4
64-65	1.9302790913326993E-4	0.001209641563901825	0.0	0.08133552664512218	3.7318729099098855E-4
66-67	1.9302790913326993E-4	0.001209641563901825	0.0	0.08744807710100906	3.8605581826653987E-4
68-69	1.9302790913326993E-4	0.0012225100911773762	0.0	0.09392094632061138	3.9892434554209124E-4
70-71	1.9302790913326993E-4	0.0012353786184529276	0.0	0.09915200265812299	4.246614000931939E-4
72-73	1.9302790913326993E-4	0.001248247145728479	0.0	0.10326349712266164	4.632669819198479E-4
74-75	1.9302790913326993E-4	0.0012739842002795817	0.0	0.10619752134148736	4.632669819198479E-4
76-77	1.9302790913326993E-4	0.001325458309381787	0.0	0.10902216307847086	4.632669819198479E-4
78-79	1.9302790913326993E-4	0.0013511953639328896	0.0	0.11099748201526799	4.632669819198479E-4
80-81	1.9302790913326993E-4	0.0013511953639328896	0.0	0.11175029086088775	4.761355091953992E-4
82-83	1.994621727710456E-4	0.0013704981548462165	0.0	0.11223286063372093	5.147410910220532E-4
84-85	2.0589643640882128E-4	0.0013898009457595437	0.0	0.1124258885428542	5.276096182976045E-4
86-87	2.0589643640882128E-4	0.0014284065275861975	0.0	0.11254813955197193	5.340438819353801E-4
88	2.0589643640882128E-4	0.0015184862185150568	0.0	0.11257387660652303	5.404781455731558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11895	0.0	52.317093	1
GTATCAA	22380	0.0	38.76992	1
CTTATAC	6835	0.0	37.077454	1
TCAACGC	27595	0.0	30.996532	4
ATCAACG	27895	0.0	30.648355	3
TATCAAC	28365	0.0	30.24528	2
CAACGCA	28355	0.0	30.161655	5
AACGCAG	29265	0.0	29.204948	6
TATACAC	10375	0.0	28.3134	3
GTGGTAT	5180	0.0	27.452957	1
TGGTATC	5050	0.0	26.982843	2
ACGCAGA	33215	0.0	25.731833	7
CGCAGAG	33805	0.0	25.332949	8
TTATACA	10865	0.0	24.457874	2
ACACATC	11745	0.0	22.112808	6
GCAGAGT	38600	0.0	22.076078	9
ACATCTC	11935	0.0	21.618557	8
TACACAT	13620	0.0	21.097033	5
GTACATG	27220	0.0	20.476261	1
TACATGG	26675	0.0	20.464947	2
>>END_MODULE