FastQCFastQC Report
Fri 27 May 2016
SRR2138892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6365949
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93400.049.8475951
GTATCAA170850.036.949551
CTTATAC53150.033.7884331
ATCAACG207350.029.8570733
TCAACGC208450.029.7194584
TATCAAC211900.029.3549042
CAACGCA213850.028.9260335
AACGCAG218800.028.3221656
TATACAC75450.026.923493
ACGCAGA249650.024.822317
CGCAGAG253650.024.4308668
GTGGTAT44550.024.1677191
TTATACA82350.023.5440272
TGGTATC43600.022.4756832
GCAGAGT296000.020.9638469
ACATCTC93100.020.185258
ACACATC91300.020.1687816
TACACAT102350.019.9268825
GTACATG202100.019.7905431
GAGTACT175650.019.7700712-13
Produced by FastQC (version 0.11.5)