##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6365949
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.843074457555346	33.0	33.0	33.0	33.0	33.0
2	32.83889409104597	33.0	33.0	33.0	33.0	33.0
3	32.83700827637796	33.0	33.0	33.0	33.0	33.0
4	36.78776267293376	37.0	37.0	37.0	37.0	37.0
5	36.79763677026002	37.0	37.0	37.0	37.0	37.0
6	36.81029474160098	37.0	37.0	37.0	37.0	37.0
7	36.79551171396441	37.0	37.0	37.0	37.0	37.0
8	36.792905503955495	37.0	37.0	37.0	37.0	37.0
9	36.79224558663602	37.0	37.0	37.0	37.0	37.0
10-11	36.77157113574111	37.0	37.0	37.0	37.0	37.0
12-13	36.75977917824977	37.0	37.0	37.0	37.0	37.0
14-15	39.1087683863003	40.0	40.0	40.0	37.0	40.0
16-17	39.06623678574868	40.0	40.0	40.0	37.0	40.0
18-19	39.05312656447609	40.0	40.0	40.0	37.0	40.0
20-21	39.100572289777745	40.0	40.0	40.0	37.0	40.0
22-23	39.13068709842533	40.0	40.0	40.0	37.0	40.0
24-25	39.134141658753165	40.0	40.0	40.0	37.0	40.0
26-27	38.86943437682659	40.0	40.0	40.0	37.0	40.0
28-29	38.86953762187655	40.0	40.0	40.0	37.0	40.0
30-31	38.88268605953805	40.0	40.0	40.0	37.0	40.0
32-33	38.87274989640714	40.0	40.0	40.0	37.0	40.0
34-35	38.85532747683776	40.0	40.0	40.0	37.0	40.0
36-37	38.81962212843829	40.0	37.0	40.0	37.0	40.0
38-39	38.77583406707598	40.0	37.0	40.0	37.0	40.0
40-41	38.73464260837817	40.0	37.0	40.0	37.0	40.0
42-43	38.67904246361527	40.0	37.0	40.0	37.0	40.0
44-45	38.60725582371735	40.0	37.0	40.0	37.0	40.0
46-47	38.52906091753905	40.0	37.0	40.0	37.0	40.0
48-49	38.4737630170443	40.0	37.0	40.0	37.0	40.0
50-51	38.41511299742912	40.0	37.0	40.0	37.0	40.0
52-53	38.35314749309066	40.0	37.0	40.0	37.0	40.0
54-55	38.28009655734378	40.0	37.0	40.0	37.0	40.0
56-57	38.17325714071696	40.0	37.0	40.0	37.0	40.0
58-59	38.04236243329218	40.0	37.0	40.0	37.0	40.0
60-61	37.8811857879329	40.0	37.0	40.0	35.0	40.0
62-63	37.70003586018635	38.5	37.0	40.0	33.0	40.0
64-65	37.48854886545716	37.0	37.0	40.0	33.0	40.0
66-67	37.26109726012645	37.0	37.0	40.0	33.0	40.0
68-69	37.00463123754484	37.0	37.0	40.0	33.0	40.0
70-71	36.75614592170773	37.0	37.0	40.0	33.0	40.0
72-73	36.51831367178005	37.0	37.0	37.0	33.0	40.0
74-75	36.260535616894586	37.0	37.0	37.0	33.0	40.0
76-77	34.55929704053497	35.0	33.0	37.0	30.0	37.0
78-79	31.45266208362237	37.0	33.0	37.0	10.5	37.0
80-81	34.532861204761076	37.0	33.0	37.0	30.0	37.0
82-83	35.56940336288583	37.0	35.0	37.0	33.0	37.0
84-85	35.69287434035095	37.0	37.0	37.0	33.0	37.0
86-87	35.65885647245061	37.0	37.0	37.0	33.0	37.0
88-89	35.582115917329034	37.0	37.0	37.0	33.0	37.0
90-91	35.5155714366458	37.0	35.0	37.0	33.0	37.0
92-93	35.450320682170016	37.0	33.0	37.0	33.0	37.0
94-95	35.404629308084566	37.0	33.0	37.0	33.0	37.0
96-97	35.380072744435665	37.0	33.0	37.0	33.0	37.0
98-99	35.33924701031681	37.0	33.0	37.0	33.0	37.0
100	35.286135060524984	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	28.0
21	97.0
22	317.0
23	919.0
24	2318.0
25	4920.0
26	8932.0
27	15160.0
28	23211.0
29	33893.0
30	47008.0
31	65138.0
32	89570.0
33	125182.0
34	184250.0
35	300375.0
36	657579.0
37	2012796.0
38	2660584.0
39	133669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.794491284802945	20.04705032980943	14.530826432948174	23.627631952439458
2	14.585272360805906	20.850968174580096	41.906430604455046	22.657328860158948
3	18.024004987804602	26.06575601636651	30.858223790502155	25.052015205326732
4	12.426659619244365	18.314441569819603	38.640949441134595	30.61794936980144
5	13.348274640391333	37.51191887388735	35.48653122081162	13.65327526490969
6	29.671648327688455	37.80199935626251	18.741950336077153	13.784401979971877
7	26.24213609000009	33.027031790546864	22.68736365936956	18.043468460083485
8	24.464616351780386	36.3345512193076	21.16601939475167	18.034813034160344
9	25.611923689618	16.88486665538791	20.995785545878547	36.50742410911555
10-11	24.0867779493678	26.76689681302819	28.76308779727893	20.38323744032508
12-13	25.181579368606315	24.340416487785248	28.769716816769975	21.708287326838466
14-15	22.33744725256203	25.70798949221868	26.571568512408756	25.38299474281054
16-17	21.702537987659028	28.978915790874225	27.207522397681792	22.111023823784954
18-19	21.855005435953068	27.76448570354554	29.074117621740292	21.3063912387611
20-21	22.595918778984405	26.905727946403722	29.077586367371154	21.420766907240715
22-23	22.229786647085568	26.969703967843262	29.00561039959958	21.79489898547159
24-25	22.10154911488971	27.11521893117319	29.03089468839537	21.75233726554173
26-27	22.074762826046115	27.24253366886008	28.989894486165106	21.6928090189287
28-29	21.812008564334313	27.24440111327216	29.08245245724818	21.86113786514534
30-31	22.154623987907645	27.116192035820706	29.040756156822123	21.688427819449522
32-33	21.766774271339806	27.153249236183115	29.169907371045095	21.910069121431984
34-35	21.843241754793073	27.309159355239927	28.990497948468363	21.85710094149864
36-37	21.967635169924023	27.316363583183872	28.97575220864746	21.740249038244645
38-39	21.855441589879153	27.34156051292789	28.956734751051105	21.84626314614185
40-41	22.04897283641488	27.216774293981743	28.861388676904028	21.87286419269935
42-43	22.17079848886657	27.250088561344572	28.697856746542012	21.881256203246846
44-45	22.056337150167092	27.53449315536259	28.424185986239635	21.984983708230686
46-47	22.353958500137963	27.370232129220895	27.926777513939673	22.34903185670147
48-49	22.19727988370024	27.546786848430678	27.932452181438585	22.3234810864305
50-51	22.122806550769404	27.758838207365592	27.873539314282215	22.244815927582792
52-53	22.256548014244775	27.688800508060574	27.932654985675363	22.12199649201929
54-55	22.236599733813087	27.64461540422657	27.761933047070187	22.35685181489016
56-57	22.427139964124603	27.85095480701003	27.486011529158688	22.235893699706676
58-59	22.102902284770327	28.027499539678814	27.813176784336065	22.056421391214798
60-61	22.398994037899183	28.12879207568188	27.449347128449936	22.022866757969005
62-63	22.167553617848693	28.33076524707406	27.391768464220473	22.109912670856783
64-65	22.19874151418185	28.193489167135127	27.39542017655112	22.2123491421319
66-67	22.31418839107813	28.0821748235063	27.325297153383627	22.27833963203194
68-69	22.3268219282633	27.927314454211384	27.45468067023662	22.2911829472887
70-71	22.35721764499892	27.645001699026	27.51659480903309	22.48118584694199
72-73	22.39077536472932	27.722029543273695	27.457980627929075	22.429214464067908
74-75	22.2685781914767	27.873541067024288	27.35489646379624	22.50298427770277
76-77	22.357755792195718	27.804589812113583	27.354836174798635	22.48281822089206
78-79	22.876944542413792	27.76175200739787	26.957212290702596	22.404091159485738
80-81	22.389949386611598	27.826180225554225	27.31872263218819	22.465147755645983
82-83	22.424703428404623	27.792861745745867	27.378129669276547	22.404305156572963
84-85	22.492207961431358	27.771462557613475	27.34466587637238	22.391663604582778
86-87	22.32294033287886	27.791982944160644	27.41870571387086	22.466371009089634
88-89	22.417477549240637	27.79362190968378	27.340790326817448	22.448110214258136
90-91	22.443598236285133	27.82308672282694	27.370081706027943	22.363233334859984
92-93	22.44404461483113	27.784385973319292	27.371868947961524	22.39970046388805
94-95	22.322721593795467	27.856879889941304	27.35227922760094	22.468119288662287
96-97	22.419964879103066	27.944108887645292	27.4545926254693	22.181333607782346
98-99	23.546975515319488	29.856809740130608	27.647614775783147	18.948599968766757
100	22.427509081480096	30.8797771135771	21.482500768176017	25.21021303676679
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2023.0
1	1791.5
2	2121.5
3	2846.0
4	3754.0
5	5880.0
6	7604.5
7	8819.0
8	9929.5
9	10198.0
10	9686.5
11	9190.0
12	10080.0
13	13060.0
14	20073.0
15	29649.0
16	37711.5
17	42808.5
18	44115.5
19	41826.5
20	37847.5
21	33438.0
22	30557.0
23	30926.0
24	34540.0
25	41828.0
26	52427.0
27	64249.5
28	75815.5
29	88904.0
30	102293.0
31	115516.0
32	130370.5
33	144669.0
34	158750.5
35	172379.0
36	187818.0
37	202016.0
38	210607.0
39	219691.0
40	230846.0
41	239126.0
42	244696.0
43	251198.0
44	256890.0
45	261519.5
46	266699.0
47	271881.5
48	277675.5
49	275162.5
50	265280.5
51	255390.5
52	242683.0
53	227284.5
54	211263.0
55	195494.5
56	179826.0
57	163473.5
58	146095.0
59	128147.0
60	108187.0
61	88360.0
62	70597.5
63	55111.5
64	41943.0
65	31652.5
66	23947.5
67	18266.0
68	14000.5
69	10199.0
70	7183.0
71	5177.0
72	3729.5
73	2565.5
74	1726.5
75	1176.5
76	784.0
77	489.0
78	324.0
79	212.5
80	143.0
81	100.5
82	65.0
83	38.5
84	22.0
85	17.0
86	14.0
87	11.0
88	8.5
89	5.5
90	3.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.4558869384596075E-4
4	0.022730310908868418
5	0.016399754380690138
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008571628804318278
26-27	0.05291016518864808
28-29	0.08578849051734022
30-31	0.07588991734069259
32-33	0.036179649913555496
34-35	0.003973178355105744
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.6521091539686247E-4
56-57	6.042499488545579E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.012462146930396382
74-75	0.02657652935459834
76-77	9.250376213754261E-4
78-79	4.907659468487351
80-81	0.0
82-83	0.012538348908912878
84-85	0.0492639220996226
86-87	0.03957738221362532
88-89	0.009012377404264802
90-91	3.0806878354419786E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	349.0
22-23	934.0
24-25	2172.0
26-27	3185.0
28-29	5588.0
30-31	13226.0
32-33	17767.0
34-35	23782.0
36-37	34765.0
38-39	41743.0
40-41	43702.0
42-43	51619.0
44-45	62569.0
46-47	60795.0
48-49	52967.0
50-51	48076.0
52-53	45895.0
54-55	50961.0
56-57	54079.0
58-59	55084.0
60-61	53652.0
62-63	54977.0
64-65	58162.0
66-67	62888.0
68-69	57764.0
70-71	52575.0
72-73	49918.0
74-75	50944.0
76-77	53004.0
78-79	52291.0
80-81	53076.0
82-83	51663.0
84-85	51048.0
86-87	53989.0
88-89	56928.0
90-91	58807.0
92-93	60448.0
94-95	71012.0
96-97	199498.0
98-99	377075.0
100-101	4116895.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.78174349447102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.3558748699846	80.19377974549978
2	6.316691549120377	11.089803945971553
3	1.3576804870380428	3.575386807818658
4	0.45891775681814895	1.6113840325627509
5	0.20233362054797124	0.8880598989624822
6	0.10968932080929683	0.5777231894018661
7	0.05687385555985521	0.34947403402077626
8	0.03992162242665543	0.28035116957918305
9	0.02598368601957091	0.20528039350897614
>10	0.07425195871578236	0.9898428740887468
>50	0.0010656213164783005	0.061534533147625234
>100	6.386513419576357E-4	0.11562263307638672
>500	5.133353948263153E-5	0.03219642332581726
>1k	2.566676176063153E-5	0.02956031903533556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5708576992998217E-5	0.0	0.0	0.0
3	0.0	1.5708576992998217E-5	0.0	3.1417153985996433E-5	0.0
4	0.0	4.712573097899465E-5	0.0	6.283430797199287E-5	0.0
5	0.0	4.712573097899465E-5	0.0	6.283430797199287E-5	0.0
6	3.1417153985996433E-5	4.712573097899465E-5	0.0	6.283430797199287E-5	0.0
7	3.1417153985996433E-5	4.712573097899465E-5	0.0	6.283430797199287E-5	0.0
8	3.1417153985996433E-5	6.283430797199287E-5	0.0	6.283430797199287E-5	0.0
9	4.712573097899465E-5	1.099600389509875E-4	0.0	1.2566861594398573E-4	0.0
10-11	5.498001947549376E-5	1.099600389509875E-4	0.0	1.4137719293698395E-4	0.0
12-13	6.283430797199287E-5	1.099600389509875E-4	0.0	1.7279434692298037E-4	0.0
14-15	6.283430797199287E-5	2.3562865489497323E-4	0.0	1.7279434692298037E-4	0.0
16-17	1.021057504544884E-4	2.4348294339147236E-4	0.0	2.3562865489497325E-4	0.0
18-19	1.099600389509875E-4	2.670458088809697E-4	0.0	2.670458088809697E-4	1.5708576992998217E-5
20-21	1.099600389509875E-4	2.827543858739679E-4	0.0	3.141715398599643E-4	1.5708576992998217E-5
22-23	1.099600389509875E-4	3.6915155933545807E-4	0.0	3.141715398599643E-4	3.927144248249554E-5
24-25	1.099600389509875E-4	4.005687133214545E-4	0.0	3.5344298234245985E-4	4.712573097899465E-5
26-27	1.099600389509875E-4	4.084230018179536E-4	0.0	3.927144248249554E-4	4.712573097899465E-5
28-29	1.099600389509875E-4	4.084230018179536E-4	0.0	5.969259257339322E-4	4.712573097899465E-5
30-31	1.099600389509875E-4	4.319858673074509E-4	0.0	0.001091746101013376	4.712573097899465E-5
32-33	1.099600389509875E-4	4.5554873279694824E-4	0.0	0.0024348294339147235	6.283430797199287E-5
34-35	1.099600389509875E-4	5.340916177619394E-4	0.0	0.004233461499613019	6.283430797199287E-5
36-37	1.099600389509875E-4	6.126345027269304E-4	0.0	0.006629019491045247	6.283430797199287E-5
38-39	1.099600389509875E-4	6.283430797199286E-4	0.0	0.010359806526882323	6.283430797199287E-5
40-41	1.099600389509875E-4	6.597602337059251E-4	0.0	0.01399634210076141	6.283430797199287E-5
42-43	1.099600389509875E-4	6.833230991954224E-4	0.0	0.017844943464045974	6.283430797199287E-5
44-45	1.099600389509875E-4	7.697202726569126E-4	0.0	0.022510390830966443	6.283430797199287E-5
46-47	1.099600389509875E-4	8.325545806289054E-4	0.0	0.02747430116075388	7.068859646849197E-5
48-49	1.099600389509875E-4	8.325545806289054E-4	0.0	0.03274452874190478	7.854288496499108E-5
50-51	1.2566861594398573E-4	9.739317735658894E-4	0.0	0.03731572464686726	7.854288496499108E-5
52-53	1.4137719293698395E-4	0.001013203216048385	0.0	0.041879066263333244	9.42514619579893E-5
54-55	1.4923148143348305E-4	0.001021057504544884	0.0	0.04637171928333073	1.099600389509875E-4
56-57	1.5708576992998216E-4	0.0010367660815378823	0.0	0.051005749496265206	1.099600389509875E-4
58-59	1.5708576992998216E-4	0.0010367660815378823	0.0	0.05581257405612266	1.099600389509875E-4
60-61	1.7279434692298037E-4	0.001091746101013376	0.0	0.060823610116889096	1.3352290444048484E-4
62-63	2.19920077901975E-4	0.0011310175434958716	0.0	0.06590533477412402	1.4137719293698395E-4
64-65	2.2777436639847412E-4	0.00123312329395036	0.0	0.0704922392560795	1.4137719293698395E-4
66-67	2.3562865489497323E-4	0.001240977582446859	0.0	0.07510270660352447	1.4923148143348305E-4
68-69	2.3562865489497323E-4	0.001240977582446859	0.0	0.07938329383411649	1.5708576992998216E-4
70-71	2.3562865489497323E-4	0.0012488318709433582	0.0	0.08375027823817	1.5708576992998216E-4
72-73	2.5133723188797147E-4	0.0012566861594398573	0.0	0.08699409938722412	1.7279434692298037E-4
74-75	2.5133723188797147E-4	0.0012802490249293546	0.0	0.08948390884061433	1.7279434692298037E-4
76-77	2.5133723188797147E-4	0.001311666178915351	0.0	0.09172238106211658	1.7279434692298037E-4
78-79	2.5133723188797147E-4	0.0013195204674118501	0.0	0.09353672170480787	1.7279434692298037E-4
80-81	2.5133723188797147E-4	0.0013195204674118501	0.0	0.0941886276500173	1.7279434692298037E-4
82-83	2.5133723188797147E-4	0.0013430833329013475	0.0	0.09470701069078624	1.885029239159786E-4
84-85	2.5133723188797147E-4	0.0013745004868873439	0.0	0.09497405649966721	1.885029239159786E-4
86-87	2.5133723188797147E-4	0.001382354775383843	0.0	0.09507616225012169	1.885029239159786E-4
88	2.5133723188797147E-4	0.001523731968320827	0.0	0.0950840165386182	1.885029239159786E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9340	0.0	49.847595	1
GTATCAA	17085	0.0	36.94955	1
CTTATAC	5315	0.0	33.788433	1
ATCAACG	20735	0.0	29.857073	3
TCAACGC	20845	0.0	29.719458	4
TATCAAC	21190	0.0	29.354904	2
CAACGCA	21385	0.0	28.926033	5
AACGCAG	21880	0.0	28.322165	6
TATACAC	7545	0.0	26.92349	3
ACGCAGA	24965	0.0	24.82231	7
CGCAGAG	25365	0.0	24.430866	8
GTGGTAT	4455	0.0	24.167719	1
TTATACA	8235	0.0	23.544027	2
TGGTATC	4360	0.0	22.475683	2
GCAGAGT	29600	0.0	20.963846	9
ACATCTC	9310	0.0	20.18525	8
ACACATC	9130	0.0	20.168781	6
TACACAT	10235	0.0	19.926882	5
GTACATG	20210	0.0	19.790543	1
GAGTACT	17565	0.0	19.77007	12-13
>>END_MODULE