FastQCFastQC Report
Fri 27 May 2016
SRR2138891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7044145
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125050.046.1206931
GTATCAA218700.035.3868981
ATCAACG249100.030.186723
TCAACGC257800.029.19914
CAACGCA261900.028.7040465
AACGCAG262700.028.6455156
ACGCAGA266300.028.242427
TATCAAC274050.027.8236222
CGCAGAG302900.024.8019688
GCAGAGT343800.021.7409279
GAGTACT218050.020.29447212-13
GTACATG213850.020.1381571
CAGAGTA331900.019.60205810-11
ACATGGG207550.019.467713
AGAGTAC310250.019.31029710-11
TACATGG214400.019.2592682
GTGGTAT64150.019.0208511
TGGTATC60450.018.9277382
GTACTTT237450.018.33493614-15
AGTACTT234250.017.66580212-13
Produced by FastQC (version 0.11.5)