##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7044145
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90140038287117	33.0	33.0	33.0	33.0	33.0
2	32.95769522064069	33.0	33.0	33.0	33.0	33.0
3	33.62937475023583	33.0	33.0	33.0	33.0	37.0
4	36.78290168643604	37.0	37.0	37.0	37.0	37.0
5	36.796997364477875	37.0	37.0	37.0	37.0	37.0
6	36.80225960709213	37.0	37.0	37.0	37.0	37.0
7	36.793129045469676	37.0	37.0	37.0	37.0	37.0
8	36.782236027225444	37.0	37.0	37.0	37.0	37.0
9	36.78711184962831	37.0	37.0	37.0	37.0	37.0
10-11	36.781997957168684	37.0	37.0	37.0	37.0	37.0
12-13	37.038829623751354	37.0	37.0	37.0	37.0	38.5
14-15	39.09243797508427	40.0	40.0	40.0	37.0	40.0
16-17	39.051545574374174	40.0	40.0	40.0	37.0	40.0
18-19	39.043903695906316	40.0	40.0	40.0	37.0	40.0
20-21	39.10208258285597	40.0	40.0	40.0	37.0	40.0
22-23	39.1308224894155	40.0	40.0	40.0	37.0	40.0
24-25	39.11415454506327	40.0	40.0	40.0	37.0	40.0
26-27	38.84988750901671	40.0	40.0	40.0	37.0	40.0
28-29	38.87929025536898	40.0	40.0	40.0	37.0	40.0
30-31	38.88472863795849	40.0	40.0	40.0	37.0	40.0
32-33	38.86698743938219	40.0	40.0	40.0	37.0	40.0
34-35	38.84506062430479	40.0	38.5	40.0	37.0	40.0
36-37	38.81175033296511	40.0	37.0	40.0	37.0	40.0
38-39	38.77422427568088	40.0	37.0	40.0	37.0	40.0
40-41	38.726117596663485	40.0	37.0	40.0	37.0	40.0
42-43	38.66858511482177	40.0	37.0	40.0	37.0	40.0
44-45	38.59992218210452	40.0	37.0	40.0	37.0	40.0
46-47	38.526603420180024	40.0	37.0	40.0	37.0	40.0
48-49	38.476823654288566	40.0	37.0	40.0	37.0	40.0
50-51	38.434277152961826	40.0	37.0	40.0	37.0	40.0
52-53	38.37319731634956	40.0	37.0	40.0	37.0	40.0
54-55	38.30205645753763	40.0	37.0	40.0	37.0	40.0
56-57	38.20144690574335	40.0	37.0	40.0	37.0	40.0
58-59	38.074208134895315	40.0	37.0	40.0	37.0	40.0
60-61	37.92209612626975	40.0	37.0	40.0	35.0	40.0
62-63	37.74152419164043	40.0	37.0	40.0	33.0	40.0
64-65	37.52905249108641	37.0	37.0	40.0	33.0	40.0
66-67	37.299078160529206	37.0	37.0	40.0	33.0	40.0
68-69	37.037221895315625	37.0	37.0	40.0	33.0	40.0
70-71	36.783471434939145	37.0	37.0	40.0	33.0	40.0
72-73	36.531742359374576	37.0	37.0	38.5	33.0	40.0
74-75	36.04402836443405	37.0	37.0	37.0	33.0	40.0
76-77	34.22140842949089	37.0	33.0	37.0	27.0	37.0
78-79	31.90197903869231	37.0	33.0	37.0	10.5	37.0
80-81	34.73055943612855	37.0	33.0	37.0	30.0	37.0
82-83	35.61229197838034	37.0	35.0	37.0	33.0	37.0
84-85	35.69580443452763	37.0	37.0	37.0	33.0	37.0
86-87	35.64986167997114	37.0	37.0	37.0	33.0	37.0
88-89	35.57243721517351	37.0	37.0	37.0	33.0	37.0
90-91	35.500560332627686	37.0	35.0	37.0	33.0	37.0
92-93	35.43097742904408	37.0	33.0	37.0	33.0	37.0
94-95	35.38494771412165	37.0	33.0	37.0	33.0	37.0
96-97	35.356754044233256	37.0	33.0	37.0	33.0	37.0
98-99	35.31210094095544	37.0	33.0	37.0	33.0	37.0
100	35.243810326518634	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	1.0
17	1.0
18	2.0
19	4.0
20	21.0
21	77.0
22	327.0
23	960.0
24	2383.0
25	5196.0
26	9843.0
27	16612.0
28	25472.0
29	37539.0
30	52927.0
31	72799.0
32	99491.0
33	138958.0
34	203912.0
35	329811.0
36	713058.0
37	2176428.0
38	2985166.0
39	173155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.97328441504754	0.0	24.81238273921201	29.214332845740454
2	14.9183847388597	23.977614132250213	38.7991800782699	22.304821050620184
3	16.445801537121355	24.757375831509513	33.457475323035126	25.339347308334006
4	12.851834392118889	23.423195213172296	37.781250266234636	25.943720128474183
5	17.074591917388055	36.52602927373086	32.46643627090532	13.932942537975759
6	27.577711702413847	37.560016155260854	20.544012083794414	14.31826005853088
7	25.79356696853443	33.288601141600736	22.598260342207656	18.319571547657173
8	23.93559813655144	32.31795091071392	22.35114727921519	21.39530367351945
9	23.801486738488027	20.503839785217338	23.843578439055193	31.85109503723944
10-11	24.409083630317607	26.20400718667875	28.880996754183336	20.50591242882031
12-13	24.92126793546526	24.433394604085322	28.70592225258314	21.93941520786628
14-15	22.340152330787106	26.75650157066636	27.00466515164937	23.898680946897166
16-17	21.183720664466733	29.042971148379255	27.958410850429683	21.81489733672433
18-19	21.27571648703636	27.98686596887187	29.861373494165754	20.876044049926012
20-21	22.14011390423254	27.29430456122338	29.77236615628015	20.79321537826393
22-23	21.934824229254783	26.99145269777155	29.632351563072383	21.441371509901288
24-25	21.634914995127048	27.26882123939006	29.77574894460755	21.320514820875346
26-27	21.558225904963876	27.459745324947704	29.58157541832969	21.40045335175873
28-29	21.480001912331005	27.452813516992997	29.68432167456452	21.38286289611148
30-31	21.7328243703934	27.316182008736984	29.620104677138652	21.33088894373096
32-33	21.54239983742716	27.61370055786659	29.454532584887477	21.389367019818774
34-35	21.733498193974572	27.53443183184214	29.339870515704693	21.39219945847859
36-37	21.638856540856075	27.57309747155958	29.37843030377288	21.409615683811463
38-39	21.521550529566785	27.793195354853335	29.333790077849446	21.35146403773043
40-41	21.856720737008388	27.53897059834674	29.10248752772778	21.50182113691709
42-43	21.71692585796792	27.6194658054567	29.069111923757383	21.59449641281799
44-45	21.826221636388414	27.95682769267011	28.657356207470247	21.559594463471228
46-47	21.951628540850614	27.784175842268027	28.33095077329928	21.933244843582074
48-49	21.86659875089379	27.977261087301457	28.32500261509412	21.831137546710632
50-51	21.90522623959856	28.190154697811558	28.041295945709372	21.863323116880508
52-53	22.11425112258816	27.96329558167691	27.971793535731255	21.950659760003678
54-55	22.04412114743673	27.908470587999133	28.084986368917598	21.96242189564654
56-57	22.066762498500765	28.220573448376157	27.83753927884534	21.875124774277737
58-59	22.075798672362538	28.017708529693223	27.952218172903017	21.95427462504122
60-61	22.04523749049508	28.075555123004165	28.02418700779146	21.85502037870929
62-63	21.98815698834364	28.233137715292315	27.87015473639105	21.908550559972994
64-65	22.0557442953514	28.018872817971157	27.84121034370033	22.084172542977118
66-67	22.0835581990278	28.126779792365262	27.765872380997504	22.023789627609432
68-69	22.09877107275176	28.156646792644647	27.78853686847249	21.956045266131103
70-71	22.125305345596384	27.747647581742214	27.880954657496016	22.24609241516539
72-73	22.135986376176323	27.877849277276834	27.822148054001584	22.16401629254526
74-75	21.99214083486517	28.053841671491682	27.809477254298372	22.144540239344774
76-77	22.300233290232406	27.8508571335006	27.55957225066137	22.28933732560563
78-79	22.460597369159487	27.910116519259343	27.51650076648952	22.112785345091645
80-81	22.11095457949379	27.99690616466004	27.672782658240575	22.219356597605593
82-83	22.211961443615447	27.820198618232645	27.779995260622464	22.187844677529444
84-85	22.29810842539496	27.817432133175956	27.780992146670414	22.10346729475867
86-87	22.107832479939628	27.884019136296313	27.805810716882846	22.20233766688122
88-89	22.26518354352402	27.74256410570932	27.73410392528921	22.258148425477444
90-91	22.246139142087948	27.749832120742756	27.845919555380718	22.158109181788575
92-93	22.217926325891046	27.81077079525292	27.794191673124363	22.177111205731663
94-95	22.253442538198897	27.719089032712606	27.799182666831634	22.228285762256863
96-97	22.271757479054973	27.977809743617748	27.990997963811488	21.759434813515792
98-99	23.309559626765214	30.04184364079429	27.52244961656017	19.126147115880325
100	22.44723857789136	30.45248126532445	21.868605820699656	25.23167433608454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1255.0
1	1174.5
2	1801.5
3	2755.0
4	4028.0
5	6810.0
6	9072.0
7	10476.0
8	11452.5
9	11485.5
10	10917.5
11	10241.5
12	11232.0
13	14461.5
14	22312.5
15	34346.0
16	45227.0
17	52150.0
18	53874.5
19	50882.5
20	45822.0
21	40424.0
22	36694.0
23	36872.5
24	41232.0
25	49927.5
26	62597.5
27	76617.5
28	90646.0
29	106403.0
30	122735.0
31	138570.0
32	155086.0
33	171585.5
34	188454.0
35	204029.5
36	220459.0
37	236072.5
38	245900.5
39	253627.0
40	263622.0
41	272515.5
42	277373.0
43	282351.0
44	286396.5
45	289573.0
46	291228.5
47	290839.0
48	289040.5
49	281699.5
50	272111.0
51	261360.5
52	248402.0
53	234167.5
54	218427.5
55	202386.5
56	186024.5
57	168256.0
58	149779.5
59	131004.0
60	110375.5
61	89863.0
62	71165.5
63	55031.0
64	41581.0
65	31184.5
66	23596.0
67	18069.0
68	13866.0
69	10202.0
70	7255.5
71	5134.0
72	3547.0
73	2367.5
74	1590.0
75	1074.0
76	712.0
77	448.5
78	287.5
79	201.0
80	138.0
81	85.0
82	59.0
83	41.0
84	27.0
85	22.5
86	20.5
87	16.5
88	13.0
89	10.0
90	6.5
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.413351797840618E-4
2	8.233788486750344E-4
3	0.004670545538173902
4	0.021081337763490103
5	0.012804960715601398
6	0.0
7	1.4196187046121282E-5
8	1.4196187046121282E-5
9	1.4196187046121282E-5
10-11	1.4196187046121282E-5
12-13	1.4196187046121282E-5
14-15	1.4196187046121282E-5
16-17	0.0
18-19	2.129428056918192E-5
20-21	6.388582098923218E-5
22-23	5.53744212485569E-4
24-25	0.014010043774108388
26-27	0.058754339801117515
28-29	0.08735869251687857
30-31	0.0728939073376237
32-33	0.0328120473854469
34-35	0.003649125819711719
36-37	7.186233246734431E-6
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	7.541727245590766E-6
50-51	7.604921875017825E-6
52-53	2.2988148153710712E-5
54-55	4.941151274349936E-4
56-57	7.160484276004602E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	8.041936446828003E-6
66-67	5.6791063163588365E-5
68-69	2.6201674286986936E-4
70-71	0.0013883378463954577
72-73	0.01720195486917467
74-75	0.07140435291435264
76-77	0.9478122849528654
78-79	3.9174283485884107
80-81	9.959341637288052E-4
82-83	0.017623039425931457
84-85	0.050393254616467244
86-87	0.0366230725714063
88-89	0.008495536145890902
90-91	3.6500436682099355E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	164.0
20-21	708.0
22-23	1256.0
24-25	2520.0
26-27	3900.0
28-29	6966.0
30-31	14543.0
32-33	19871.0
34-35	27655.0
36-37	38171.0
38-39	44896.0
40-41	47031.0
42-43	57426.0
44-45	68532.0
46-47	65838.0
48-49	57221.0
50-51	50348.0
52-53	48341.0
54-55	51222.0
56-57	53352.0
58-59	52186.0
60-61	51220.0
62-63	50362.0
64-65	53381.0
66-67	56518.0
68-69	56227.0
70-71	54791.0
72-73	53167.0
74-75	54501.0
76-77	56618.0
78-79	57385.0
80-81	56839.0
82-83	55700.0
84-85	56485.0
86-87	59183.0
88-89	63501.0
90-91	66002.0
92-93	68825.0
94-95	88466.0
96-97	324646.0
98-99	1241685.0
100-101	3706496.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.15233839372418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.59161918696935	76.28935874250486
2	7.353259881732586	12.52294547492579
3	1.7865555244933444	4.56388141742504
4	0.592441569715391	2.017911400916564
5	0.2680269351051523	1.1411560138353343
6	0.1446376115452738	0.7389738505657909
7	0.07824582773133978	0.46639706406132647
8	0.046574949151782556	0.3172772664674468
9	0.030972575903270693	0.2373648537816552
>10	0.10448033306466928	1.3696250866753765
>50	0.0024548479091942455	0.13958073649615924
>100	6.251160197305871E-4	0.11483809383441665
>500	7.923055432484862E-5	0.045944769049510614
>1k	2.6410104621486163E-5	0.034745229460720825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4196187046121282E-5	0.0
2	0.0	1.4196187046121282E-5	0.0	1.4196187046121282E-5	0.0
3	0.0	1.4196187046121282E-5	0.0	2.8392374092242564E-5	0.0
4	0.0	1.4196187046121282E-5	0.0	2.8392374092242564E-5	0.0
5	1.4196187046121282E-5	4.258856113836385E-5	0.0	2.8392374092242564E-5	0.0
6	1.4196187046121282E-5	4.258856113836385E-5	0.0	2.8392374092242564E-5	0.0
7	1.4196187046121282E-5	4.258856113836385E-5	0.0	2.8392374092242564E-5	1.4196187046121282E-5
8	1.4196187046121282E-5	7.09809352306064E-5	0.0	2.8392374092242564E-5	1.4196187046121282E-5
9	1.4196187046121282E-5	1.2776568341509153E-4	0.0	4.258856113836385E-5	1.4196187046121282E-5
10-11	1.4196187046121282E-5	1.2776568341509153E-4	0.0	7.098093523060642E-5	1.4196187046121282E-5
12-13	1.4196187046121282E-5	1.2776568341509153E-4	0.0	9.227521579978834E-5	1.4196187046121282E-5
14-15	1.4196187046121282E-5	2.058447121687586E-4	0.0	1.2776568341509153E-4	1.4196187046121282E-5
16-17	1.4196187046121282E-5	2.271389927379405E-4	0.0	1.419618704612128E-4	2.1294280569181925E-5
18-19	1.4196187046121282E-5	2.5553136683018307E-4	0.0	2.058447121687586E-4	4.258856113836385E-5
20-21	1.4196187046121282E-5	2.5553136683018307E-4	0.0	3.0521802149160754E-4	4.968665466142449E-5
22-23	1.4196187046121282E-5	2.839237409224256E-4	0.0	4.187875178605778E-4	6.388284170754577E-5
24-25	1.4196187046121282E-5	3.0521802149160754E-4	0.0	5.039646401373056E-4	7.09809352306064E-5
26-27	1.4196187046121282E-5	3.265123020607895E-4	0.0	5.678474818448513E-4	7.09809352306064E-5
28-29	1.4196187046121282E-5	3.4780658262997143E-4	0.0	9.298502515209439E-4	7.09809352306064E-5
30-31	1.4196187046121282E-5	4.187875178605778E-4	0.0	0.0016680519779192506	9.937330932284898E-5
32-33	1.4196187046121282E-5	4.329837049066991E-4	0.0	0.0030237878408238333	9.937330932284898E-5
34-35	4.258856113836385E-5	5.252589207064874E-4	0.0	0.005046744494896116	9.937330932284898E-5
36-37	4.258856113836385E-5	6.033379494601545E-4	0.0	0.00785758953002813	9.937330932284898E-5
38-39	4.968665466142449E-5	6.885150717368823E-4	0.0	0.011782835248280665	1.1356949636897026E-4
40-41	7.09809352306064E-5	8.304769421980951E-4	0.0	0.016566950282823536	1.1356949636897026E-4
42-43	7.09809352306064E-5	9.014578774287013E-4	0.0	0.021088435857013164	1.1356949636897026E-4
44-45	7.09809352306064E-5	9.653407191362472E-4	0.0	0.025595725244156674	1.1356949636897026E-4
46-47	7.09809352306064E-5	9.724388126593079E-4	0.0	0.03099737441520582	1.1356949636897026E-4
48-49	7.09809352306064E-5	9.795369061823685E-4	0.0	0.03620737506113233	1.2776568341509153E-4
50-51	7.09809352306064E-5	0.001185381618351127	0.0	0.041431571894104964	1.2776568341509153E-4
52-53	7.09809352306064E-5	0.001206675898920309	0.0	0.04693969246800002	1.4196187046121284E-4
54-55	7.09809352306064E-5	0.001206675898920309	0.0	0.052490401603033435	1.5615805750733412E-4
56-57	7.09809352306064E-5	0.0012208720859664302	0.0	0.057863658399990345	1.5615805750733412E-4
58-59	7.09809352306064E-5	0.0012634606471047943	0.0	0.06338597516093153	1.5615805750733412E-4
60-61	8.51771222767277E-5	0.001306049208243158	0.0	0.06857468152628886	1.5615805750733412E-4
62-63	8.51771222767277E-5	0.0013486377693815218	0.0	0.07369950504993864	1.703542445534554E-4
64-65	8.51771222767277E-5	0.0015331882009810985	0.0	0.07956253029998672	1.7745233807651604E-4
66-67	8.51771222767277E-5	0.001540286294504159	0.0	0.08497127756455894	1.8455043159957668E-4
68-69	9.937330932284898E-5	0.0016041691362117048	0.0	0.08931531080067204	1.8455043159957668E-4
70-71	9.937330932284898E-5	0.0016467576973500687	0.0	0.0933825183893858	1.9164852512263731E-4
72-73	9.937330932284898E-5	0.0016751500714423115	0.0	0.09671152425170124	1.9874661864569795E-4
74-75	9.937330932284898E-5	0.0017035424455345539	0.0	0.10000503964640137	1.9874661864569795E-4
76-77	9.937330932284898E-5	0.001760327193719039	0.0	0.10214866389036568	1.9874661864569795E-4
78-79	9.937330932284898E-5	0.0017887195678112815	0.0	0.10349020356622415	1.9874661864569795E-4
80-81	1.0647140284590962E-4	0.0017887195678112815	0.0	0.10400836439340758	2.058447121687586E-4
82-83	1.1356949636897026E-4	0.0017887195678112815	0.0	0.10439166144365285	2.1294280569181923E-4
84-85	1.1356949636897026E-4	0.0017958176613343422	0.0	0.10464719281048303	2.1294280569181923E-4
86-87	1.1356949636897026E-4	0.001802915754857403	0.0	0.1046897813716214	2.1294280569181923E-4
88	1.1356949636897026E-4	0.0018313081289496455	0.0	0.10469687946514446	2.271389927379405E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12505	0.0	46.120693	1
GTATCAA	21870	0.0	35.386898	1
ATCAACG	24910	0.0	30.18672	3
TCAACGC	25780	0.0	29.1991	4
CAACGCA	26190	0.0	28.704046	5
AACGCAG	26270	0.0	28.645515	6
ACGCAGA	26630	0.0	28.24242	7
TATCAAC	27405	0.0	27.823622	2
CGCAGAG	30290	0.0	24.801968	8
GCAGAGT	34380	0.0	21.740927	9
GAGTACT	21805	0.0	20.294472	12-13
GTACATG	21385	0.0	20.138157	1
CAGAGTA	33190	0.0	19.602058	10-11
ACATGGG	20755	0.0	19.46771	3
AGAGTAC	31025	0.0	19.310297	10-11
TACATGG	21440	0.0	19.259268	2
GTGGTAT	6415	0.0	19.020851	1
TGGTATC	6045	0.0	18.927738	2
GTACTTT	23745	0.0	18.334936	14-15
AGTACTT	23425	0.0	17.665802	12-13
>>END_MODULE