FastQCFastQC Report
Fri 27 May 2016
SRR2138890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5690699
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88500.050.4374581
CTTATAC56250.040.534351
GTATCAA161400.036.719161
TATACAC79950.030.8251883
ATCAACG192400.030.4107573
TCAACGC192600.030.3791754
CAACGCA196950.029.7001065
TATCAAC199950.029.3882332
AACGCAG202400.028.9987876
TTATACA83700.028.7931562
ACGCAGA228950.025.617677
CGCAGAG230950.025.3958238
GTGGTAT39650.025.2639681
CTCGTAT111600.023.6873999
TACACAT105350.023.625475
TCTCGTA111650.023.4141338
TGGTATC39950.023.3956412
AATCTCG113050.023.1612326
ACATCTC99150.023.070248
ACACATC100150.022.9235466
Produced by FastQC (version 0.11.5)