##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5690699
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84615932067396	33.0	33.0	33.0	33.0	33.0
2	32.84555306826103	33.0	33.0	33.0	33.0	33.0
3	32.84434144206186	33.0	33.0	33.0	33.0	33.0
4	36.79239017913265	37.0	37.0	37.0	37.0	37.0
5	36.79496420387021	37.0	37.0	37.0	37.0	37.0
6	36.81270613680323	37.0	37.0	37.0	37.0	37.0
7	36.79985428855049	37.0	37.0	37.0	37.0	37.0
8	36.7650824617503	37.0	37.0	37.0	37.0	37.0
9	36.7658169936593	37.0	37.0	37.0	37.0	37.0
10-11	36.77083869661706	37.0	37.0	37.0	37.0	37.0
12-13	36.759563104637934	37.0	37.0	37.0	37.0	37.0
14-15	39.1040062038073	40.0	40.0	40.0	37.0	40.0
16-17	39.06956974881293	40.0	40.0	40.0	37.0	40.0
18-19	39.050841030249536	40.0	40.0	40.0	37.0	40.0
20-21	39.09192692490529	40.0	40.0	40.0	37.0	40.0
22-23	39.13548662737047	40.0	40.0	40.0	37.0	40.0
24-25	39.13673327145997	40.0	40.0	40.0	37.0	40.0
26-27	38.84810998431733	40.0	40.0	40.0	37.0	40.0
28-29	38.859794101354666	40.0	40.0	40.0	37.0	40.0
30-31	38.85837275596208	40.0	40.0	40.0	37.0	40.0
32-33	38.85653473368964	40.0	40.0	40.0	37.0	40.0
34-35	38.83921780038244	40.0	38.5	40.0	37.0	40.0
36-37	38.810298198087835	40.0	37.0	40.0	37.0	40.0
38-39	38.764664122417905	40.0	37.0	40.0	37.0	40.0
40-41	38.74201448812454	40.0	37.0	40.0	37.0	40.0
42-43	38.69654035377906	40.0	37.0	40.0	37.0	40.0
44-45	38.62767076603129	40.0	37.0	40.0	37.0	40.0
46-47	38.556756779598786	40.0	37.0	40.0	37.0	40.0
48-49	38.510503926560794	40.0	37.0	40.0	37.0	40.0
50-51	38.45333863174351	40.0	37.0	40.0	37.0	40.0
52-53	38.382890856949594	40.0	37.0	40.0	37.0	40.0
54-55	38.31753081707126	40.0	37.0	40.0	37.0	40.0
56-57	38.216085266049426	40.0	37.0	40.0	37.0	40.0
58-59	38.086725665341476	40.0	37.0	40.0	37.0	40.0
60-61	37.93614768497872	40.0	37.0	40.0	35.0	40.0
62-63	37.760018161270665	40.0	37.0	40.0	33.0	40.0
64-65	37.55677700748434	37.0	37.0	40.0	33.0	40.0
66-67	37.33980241586666	37.0	37.0	40.0	33.0	40.0
68-69	37.090081221635515	37.0	37.0	40.0	33.0	40.0
70-71	36.84654231847463	37.0	37.0	40.0	33.0	40.0
72-73	36.61185700361223	37.0	37.0	38.5	33.0	40.0
74-75	36.351022586496576	37.0	37.0	37.0	33.0	40.0
76-77	34.67864758853797	37.0	33.0	37.0	30.0	37.0
78-79	30.848877711177465	37.0	33.0	37.0	8.5	37.0
80-81	34.39051963959952	37.0	33.0	37.0	30.0	37.0
82-83	35.58309124152268	37.0	35.0	37.0	33.0	37.0
84-85	35.73251918181566	37.0	37.0	37.0	33.0	37.0
86-87	35.69895508371333	37.0	37.0	37.0	33.0	37.0
88-89	35.620165756571396	37.0	37.0	37.0	33.0	37.0
90-91	35.553239660212384	37.0	37.0	37.0	33.0	37.0
92-93	35.486673611043415	37.0	33.0	37.0	33.0	37.0
94-95	35.442089072445825	37.0	33.0	37.0	33.0	37.0
96-97	35.41691458502027	37.0	33.0	37.0	33.0	37.0
98-99	35.37776577737208	37.0	33.0	37.0	33.0	37.0
100	35.32408761318982	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	1.0
16	0.0
17	0.0
18	6.0
19	7.0
20	17.0
21	78.0
22	314.0
23	869.0
24	2095.0
25	4441.0
26	8052.0
27	13288.0
28	20282.0
29	29465.0
30	41499.0
31	57203.0
32	78775.0
33	110523.0
34	164733.0
35	269741.0
36	592700.0
37	1816201.0
38	2361013.0
39	119394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82951865842843	20.194900485862984	14.583287571526801	23.392293284181783
2	14.342385706922823	20.798394010999353	42.010322457750796	22.84889782432703
3	17.753181645730997	26.81235147882848	30.918042057136514	24.51642481830401
4	12.78856411050531	18.46889403817412	38.58382313160248	30.15871871971809
5	13.069518949663589	38.052286230802785	35.473635258300966	13.404559561232658
6	29.108392483946172	38.468701296624545	18.75040306999193	13.672503149437354
7	25.738613129951172	33.56703631662824	22.773072341376693	17.921278212043898
8	24.083614332791104	36.42280148712838	21.751071353448847	17.742512826631668
9	25.16659201268596	16.929923722902934	21.041474869783134	36.86200939462797
10-11	23.730397618991972	26.739193199288874	29.061333238675953	20.4690759430432
12-13	25.165291645191566	24.32303131829675	29.067228823735007	21.444448212776674
14-15	22.066059020166065	26.040447755187895	26.810669480146466	25.082823744499578
16-17	21.73569538645428	29.075619708580614	27.09416716645881	22.09451773850629
18-19	21.78766615489591	27.835350279464787	29.04412972817575	21.332853837463553
20-21	22.377428706213305	26.840753947483687	29.324773302008918	21.457044044294094
22-23	21.928352559472973	27.139273617203415	29.463712274235025	21.468661549088583
24-25	21.8637638727319	27.38879071396584	29.201944083216397	21.54550133008587
26-27	22.126943404206173	27.460495941861247	28.881013835821417	21.53154681811117
28-29	21.481879734906425	27.456248458801795	29.58831157043354	21.473560235858237
30-31	22.459386062067786	27.470218560550247	28.901514857760446	21.16888051962152
32-33	21.41176059312159	28.191019417132896	28.916919615301495	21.48030037444402
34-35	21.69132754910913	28.171333192254693	29.00172347825579	21.135615780380387
36-37	21.620526845263154	28.13839323619484	28.808034485839762	21.433045432702247
38-39	21.60766229006742	27.668240118700027	29.086299841197594	21.63779775003496
40-41	21.803997458425364	27.373492634503332	29.13655414930314	21.685955757768163
42-43	21.99987831910596	27.328026006194435	29.004116566653348	21.66797910804626
44-45	21.826920673981558	27.652154115509454	28.71770884784552	21.80321636266347
46-47	22.186103704107943	27.461139800197	28.16865748535483	22.18409901034023
48-49	22.058663408317226	27.611972185339862	28.174974489485173	22.154389916857742
50-51	21.920294956627973	27.877733159733083	28.15605047208672	22.04592141155223
52-53	22.03210229434191	27.877783011892358	28.242783944605865	21.84733074915987
54-55	22.026868691928843	27.85222536135269	27.98398054986702	22.136925396851446
56-57	22.242415844065473	28.129247184941008	27.664442043546757	21.963894927446763
58-59	21.90875278381412	28.33384319208535	28.019089737389013	21.738314286711518
60-61	22.20484386281241	28.487976433970523	27.574029622610418	21.73315008060665
62-63	21.935579139351532	28.704823652802048	27.54166230033309	21.81793490751333
64-65	21.96581542781498	28.538245493971843	27.572674509405626	21.92326456880755
66-67	22.128990184984314	28.324648762609208	27.553831529588663	21.992529522817815
68-69	22.15979979899033	28.145912720875465	27.642439610457124	22.05184786967708
70-71	22.15622154626521	27.88059955426869	27.73414915214516	22.22902974732093
72-73	22.200962327525083	27.86956730002437	27.71259032019156	22.216880052258986
74-75	22.084504970405447	28.017732498569377	27.646673939532047	22.25108859149313
76-77	22.231858701624663	27.923974364985753	27.59797332785116	22.246193605538423
78-79	22.843676943282837	27.81028188379466	27.149501175798264	22.196539997124244
80-81	22.25066572363806	27.888394843272437	27.579850958097563	22.28108847499194
82-83	22.27017886893141	27.883154596511183	27.65067181435209	22.195994720205313
84-85	22.37108530033759	27.817803944085295	27.62586033943452	22.185250416142598
86-87	22.24089298289029	27.82258998998268	27.674268984890816	22.262248042236216
88-89	22.29196461555906	27.759985936202664	27.66106842796373	22.286981020274546
90-91	22.379167738818307	27.69818915901968	27.703141111199557	22.219501990962456
92-93	22.38467979748996	27.678957544994	27.67154109690556	22.264821560610482
94-95	22.24248058260496	27.738903412855016	27.73992444371627	22.278691560823756
96-97	22.330640224990375	27.82091129509716	27.77575569491485	22.072692784997614
98-99	23.487620341554567	29.7211068268726	27.95825464468213	18.8330181868907
100	22.342702887408166	30.716992995045278	21.890859388347856	25.0494447291987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1026.0
1	951.0
2	1336.0
3	1946.0
4	2748.0
5	4554.5
6	6070.0
7	7190.5
8	8247.0
9	8632.5
10	8339.5
11	7948.5
12	8713.5
13	11302.0
14	17457.5
15	25895.5
16	33123.0
17	38143.5
18	39622.0
19	37560.5
20	33989.5
21	30073.5
22	27646.5
23	28267.0
24	32048.0
25	38841.0
26	48185.5
27	59194.5
28	70728.5
29	83215.5
30	95765.5
31	108145.0
32	121799.5
33	135109.5
34	147890.0
35	160551.5
36	176774.5
37	194357.0
38	208304.0
39	218190.5
40	225508.0
41	229618.0
42	229766.0
43	231131.0
44	234403.0
45	240988.0
46	248294.5
47	253198.5
48	250071.0
49	240703.5
50	232656.5
51	220875.5
52	205615.5
53	191268.0
54	177765.5
55	163694.5
56	149663.5
57	134992.5
58	119369.0
59	103540.5
60	87293.5
61	72016.0
62	57683.5
63	44836.0
64	34107.5
65	25607.0
66	19557.5
67	15066.5
68	11376.5
69	8179.5
70	5728.5
71	4069.0
72	2804.5
73	1874.0
74	1299.5
75	885.0
76	564.5
77	364.0
78	228.0
79	135.5
80	89.0
81	57.0
82	38.5
83	24.5
84	16.0
85	12.0
86	10.5
87	11.5
88	10.0
89	7.0
90	5.0
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	2.1087040449688165E-4
4	0.02551531894412268
5	0.01653575421929714
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008788825184554342
26-27	0.05197682037779728
28-29	0.08312583395223229
30-31	0.07369863870186835
32-33	0.03429343781035958
34-35	0.0038852645104009154
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.262919216742E-4
56-57	6.294327803556546E-4
58-59	0.0
60-61	0.0
62-63	9.970694135796068E-6
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.0424999064356333E-5
72-73	0.014241808433887377
74-75	0.02829622009040127
76-77	8.047224115406207E-4
78-79	5.5909076961626365
80-81	0.0
82-83	0.012840257128362832
84-85	0.04904708206764427
86-87	0.038354299936275815
88-89	0.00891481143345139
90-91	4.627990549643298E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	332.0
22-23	753.0
24-25	1810.0
26-27	2609.0
28-29	4690.0
30-31	11253.0
32-33	14879.0
34-35	21853.0
36-37	39018.0
38-39	48194.0
40-41	44972.0
42-43	45082.0
44-45	54094.0
46-47	53093.0
48-49	46303.0
50-51	41190.0
52-53	39702.0
54-55	44813.0
56-57	49259.0
58-59	49853.0
60-61	50003.0
62-63	51478.0
64-65	54567.0
66-67	59370.0
68-69	53184.0
70-71	47026.0
72-73	44346.0
74-75	45716.0
76-77	47346.0
78-79	47936.0
80-81	47312.0
82-83	45968.0
84-85	46125.0
86-87	48862.0
88-89	52278.0
90-91	54144.0
92-93	55703.0
94-95	60423.0
96-97	174051.0
98-99	332896.0
100-101	3658125.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.57364103283317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.645142765336	81.17343977699619
2	6.2638644466931375	11.096265619594483
3	1.2686491022075168	3.37106610526664
4	0.41002781559591733	1.4527062620827798
5	0.16949479937674486	0.7506385758463937
6	0.07936531841633514	0.4217805134318984
7	0.053685815513652806	0.3328603706302833
8	0.029972836822041132	0.21238426315289247
9	0.018027337660016275	0.14370722412083609
>10	0.05991741603286959	0.8046934935600949
>50	0.0010453035270502164	0.06264079159056145
>100	8.070428187294945E-4	0.1778170037269419
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.757253370807347E-5	0.0	0.0	0.0
3	0.0	1.757253370807347E-5	0.0	0.0	0.0
4	0.0	3.514506741614694E-5	0.0	0.0	0.0
5	0.0	3.514506741614694E-5	0.0	0.0	0.0
6	0.0	3.514506741614694E-5	0.0	0.0	0.0
7	0.0	3.514506741614694E-5	0.0	0.0	0.0
8	0.0	5.271760112422042E-5	0.0	0.0	0.0
9	0.0	1.4058026966458776E-4	0.0	1.757253370807347E-5	0.0
10-11	8.786266854036735E-6	1.4058026966458776E-4	0.0	1.757253370807347E-5	0.0
12-13	1.757253370807347E-5	1.4058026966458776E-4	0.0	1.757253370807347E-5	0.0
14-15	1.757253370807347E-5	1.7572533708073472E-4	0.0	1.757253370807347E-5	0.0
16-17	1.757253370807347E-5	1.7572533708073472E-4	0.0	1.757253370807347E-5	0.0
18-19	1.757253370807347E-5	1.9329787078880818E-4	0.0	3.514506741614694E-5	1.757253370807347E-5
20-21	3.514506741614694E-5	2.1087040449688167E-4	0.0	3.514506741614694E-5	1.757253370807347E-5
22-23	5.271760112422042E-5	2.8116053932917553E-4	0.0	5.271760112422041E-5	1.757253370807347E-5
24-25	7.029013483229388E-5	2.98733073037249E-4	0.0	1.0543520224844084E-4	1.757253370807347E-5
26-27	8.786266854036736E-5	2.98733073037249E-4	0.0	1.493665365186245E-4	1.757253370807347E-5
28-29	1.0543520224844084E-4	2.98733073037249E-4	0.0	4.129545421397266E-4	1.757253370807347E-5
30-31	1.230077359565143E-4	3.3387814045339595E-4	0.0	0.001194932292148996	1.757253370807347E-5
32-33	1.3179400281055102E-4	3.426644073074327E-4	0.0	0.003031262064642674	2.6358800562110206E-5
34-35	1.493665365186245E-4	4.3931334270183684E-4	0.0	0.005640783320291585	3.514506741614694E-5
36-37	1.5815280337266126E-4	5.096034775341307E-4	0.0	0.009225580196738572	3.514506741614694E-5
38-39	1.5815280337266126E-4	5.271760112422042E-4	0.0	0.014769714581635753	4.393133427018368E-5
40-41	1.7572533708073472E-4	6.150386797825715E-4	0.0	0.02127155205362294	5.271760112422042E-5
42-43	1.7572533708073472E-4	6.238249466366083E-4	0.0	0.02775581699190205	5.271760112422042E-5
44-45	1.7572533708073472E-4	6.853288146148654E-4	0.0	0.035241716351541344	5.271760112422042E-5
46-47	1.7572533708073472E-4	7.468326825931227E-4	0.0	0.04282426464657505	5.271760112422042E-5
48-49	1.7572533708073472E-4	7.556189494471593E-4	0.0	0.05009929360171747	7.029013483229388E-5
50-51	1.7572533708073472E-4	8.259090842794532E-4	0.0	0.05804207883776667	7.029013483229388E-5
52-53	1.9329787078880818E-4	8.259090842794532E-4	0.0	0.06626602461314507	8.786266854036736E-5
54-55	1.9329787078880818E-4	8.259090842794532E-4	0.0	0.07371677890536821	1.0543520224844084E-4
56-57	1.9329787078880818E-4	8.434816179875267E-4	0.0	0.07931363089138962	1.0543520224844084E-4
58-59	1.9329787078880818E-4	8.522678848415634E-4	0.0	0.08546401768921533	1.0543520224844084E-4
60-61	1.9329787078880818E-4	8.874129522577104E-4	0.0	0.09057762499826472	1.0543520224844084E-4
62-63	1.9329787078880818E-4	8.961992191117471E-4	0.0	0.0957175911078762	1.0543520224844084E-4
64-65	1.9329787078880818E-4	9.752756207980777E-4	0.0	0.10141109202929201	1.0543520224844084E-4
66-67	1.9329787078880818E-4	0.001001634421360188	0.0	0.10677071481025441	1.0543520224844084E-4
68-69	1.9329787078880818E-4	0.001001634421360188	0.0	0.1110935581024405	1.0543520224844084E-4
70-71	1.9329787078880818E-4	0.0010104206882142247	0.0	0.11487165284967629	1.0543520224844084E-4
72-73	2.2844293820495514E-4	0.001027993221922298	0.0	0.11796441878229721	1.4058026966458776E-4
74-75	2.2844293820495514E-4	0.0010543520224844082	0.0	0.12038064216715733	1.4058026966458776E-4
76-77	2.2844293820495514E-4	0.0011158558904626656	0.0	0.1228407968862876	1.4058026966458776E-4
78-79	2.2844293820495514E-4	0.0011422146910247757	0.0	0.12451897385540862	1.4058026966458776E-4
80-81	2.460154719130286E-4	0.0011422146910247757	0.0	0.12514279880204524	1.4058026966458776E-4
82-83	2.460154719130286E-4	0.0011510009578788124	0.0	0.12564361601272533	1.5815280337266126E-4
84-85	2.5480173876706535E-4	0.0011773597584409227	0.0	0.12585448641722222	1.5815280337266126E-4
86-87	2.635880056211021E-4	0.001194932292148996	0.0	0.1258808452177843	1.5815280337266126E-4
88	2.635880056211021E-4	0.0012476498932732164	0.0	0.12588963148463836	1.5815280337266126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8850	0.0	50.437458	1
CTTATAC	5625	0.0	40.53435	1
GTATCAA	16140	0.0	36.71916	1
TATACAC	7995	0.0	30.825188	3
ATCAACG	19240	0.0	30.410757	3
TCAACGC	19260	0.0	30.379175	4
CAACGCA	19695	0.0	29.700106	5
TATCAAC	19995	0.0	29.388233	2
AACGCAG	20240	0.0	28.998787	6
TTATACA	8370	0.0	28.793156	2
ACGCAGA	22895	0.0	25.61767	7
CGCAGAG	23095	0.0	25.395823	8
GTGGTAT	3965	0.0	25.263968	1
CTCGTAT	11160	0.0	23.687399	9
TACACAT	10535	0.0	23.62547	5
TCTCGTA	11165	0.0	23.414133	8
TGGTATC	3995	0.0	23.395641	2
AATCTCG	11305	0.0	23.161232	6
ACATCTC	9915	0.0	23.07024	8
ACACATC	10015	0.0	22.923546	6
>>END_MODULE