FastQCFastQC Report
Fri 27 May 2016
SRR2138889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421220
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98850.050.917691
CTTATAC61350.040.3671841
GTATCAA179850.036.782441
TATACAC86950.031.7816643
TCAACGC216850.030.0974064
ATCAACG217150.030.0174373
TTATACA88000.029.3888592
TATCAAC224100.029.1618122
CAACGCA223600.029.1442835
AACGCAG228750.028.4492666
ACGCAGA261650.024.888167
CGCAGAG262700.024.740528
ACACATC111900.022.6899036
GTGGTAT46200.022.2819291
TACACAT121000.022.170145
TGGTATC44800.021.6598172
GCAGAGT300000.021.5660389
ACATCTC117550.021.5275638
GAGTACT180850.020.45254512-13
CACATCT127250.019.5551197
Produced by FastQC (version 0.11.5)