##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6421220
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8376624691258	33.0	33.0	33.0	33.0	33.0
2	32.83506109430918	33.0	33.0	33.0	33.0	33.0
3	32.834221222758295	33.0	33.0	33.0	33.0	33.0
4	36.78340720299258	37.0	37.0	37.0	37.0	37.0
5	36.79197878284812	37.0	37.0	37.0	37.0	37.0
6	36.80367453536867	37.0	37.0	37.0	37.0	37.0
7	36.788219216908935	37.0	37.0	37.0	37.0	37.0
8	36.784582369082514	37.0	37.0	37.0	37.0	37.0
9	36.78566985712995	37.0	37.0	37.0	37.0	37.0
10-11	36.76379130445616	37.0	37.0	37.0	37.0	37.0
12-13	36.75125669265342	37.0	37.0	37.0	37.0	37.0
14-15	39.08393879979194	40.0	40.0	40.0	37.0	40.0
16-17	39.038278785028396	40.0	40.0	40.0	37.0	40.0
18-19	39.020615786408186	40.0	40.0	40.0	37.0	40.0
20-21	39.07345274044255	40.0	40.0	40.0	37.0	40.0
22-23	39.105087411619586	40.0	40.0	40.0	37.0	40.0
24-25	39.11008081400967	40.0	40.0	40.0	37.0	40.0
26-27	38.838108790282504	40.0	40.0	40.0	37.0	40.0
28-29	38.834386798601955	40.0	40.0	40.0	37.0	40.0
30-31	38.85413411351813	40.0	38.5	40.0	37.0	40.0
32-33	38.83709390299417	40.0	37.0	40.0	37.0	40.0
34-35	38.8164850184772	40.0	37.0	40.0	37.0	40.0
36-37	38.7803468797978	40.0	37.0	40.0	37.0	40.0
38-39	38.724515976260705	40.0	37.0	40.0	37.0	40.0
40-41	38.69450399841584	40.0	37.0	40.0	37.0	40.0
42-43	38.64582198041763	40.0	37.0	40.0	37.0	40.0
44-45	38.57574386352388	40.0	37.0	40.0	37.0	40.0
46-47	38.500382315632734	40.0	37.0	40.0	37.0	40.0
48-49	38.43939072281176	40.0	37.0	40.0	37.0	40.0
50-51	38.37157819700208	40.0	37.0	40.0	37.0	40.0
52-53	38.30776515364869	40.0	37.0	40.0	37.0	40.0
54-55	38.23412690657257	40.0	37.0	40.0	37.0	40.0
56-57	38.12648465273591	40.0	37.0	40.0	37.0	40.0
58-59	37.99261605856621	40.0	37.0	40.0	37.0	40.0
60-61	37.827227847720124	40.0	37.0	40.0	33.0	40.0
62-63	37.64478250754228	38.5	37.0	40.0	33.0	40.0
64-65	37.43876592807405	37.0	37.0	40.0	33.0	40.0
66-67	37.21481946524831	37.0	37.0	40.0	33.0	40.0
68-69	36.95769992361183	37.0	37.0	40.0	33.0	40.0
70-71	36.708196401215076	37.0	37.0	40.0	33.0	40.0
72-73	36.47232094967347	37.0	37.0	37.0	33.0	40.0
74-75	36.21015791198785	37.0	37.0	37.0	33.0	40.0
76-77	34.513365425557346	35.0	33.0	37.0	30.0	37.0
78-79	31.36012957303538	37.0	33.0	37.0	8.5	37.0
80-81	34.473789140583825	37.0	33.0	37.0	30.0	37.0
82-83	35.52554441476311	37.0	35.0	37.0	33.0	37.0
84-85	35.650027290241255	37.0	37.0	37.0	33.0	37.0
86-87	35.614952882426074	37.0	37.0	37.0	33.0	37.0
88-89	35.5349175163699	37.0	37.0	37.0	33.0	37.0
90-91	35.469030002650825	37.0	33.0	37.0	33.0	37.0
92-93	35.398939537561674	37.0	33.0	37.0	33.0	37.0
94-95	35.35171850393474	37.0	33.0	37.0	33.0	37.0
96-97	35.32993514038894	37.0	33.0	37.0	33.0	37.0
98-99	35.29130358094195	37.0	33.0	37.0	33.0	37.0
100	35.23920663721249	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	17.0
21	87.0
22	324.0
23	925.0
24	2412.0
25	5106.0
26	9764.0
27	16243.0
28	24783.0
29	36167.0
30	49838.0
31	68237.0
32	93471.0
33	130135.0
34	191463.0
35	312964.0
36	683494.0
37	2061167.0
38	2611470.0
39	123148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55294476750524	20.063118846574326	14.622890977104039	23.761045408816393
2	14.476548070304396	20.66163439346417	42.14876612232567	22.71305141390577
3	17.979569255303325	26.062320078552236	30.88465482724816	25.07345583889628
4	12.330222594057792	18.343896222360716	38.43885863787409	30.8870225457074
5	13.309769254156864	37.53350350392759	35.471083251923915	13.685643989991627
6	29.6937653592308	37.71459317699752	18.668025702280875	13.923615761490806
7	26.191035348422886	33.02476787900119	22.51407053488278	18.270126237693148
8	24.425825621922314	36.40502895088472	21.147943848676732	18.021201578516234
9	25.365101958817792	16.974406732676968	20.876531251070666	36.78396005743457
10-11	24.068938612911563	26.72971024197894	28.650692547522123	20.550658597587372
12-13	25.16877633845282	24.241982053254677	28.652997405477464	21.936244202815043
14-15	22.350199806267344	25.69665889036663	26.38199594469587	25.57114535867016
16-17	21.787682091565156	29.028479011776575	26.980464771492024	22.203374125166246
18-19	21.818844394055958	27.71212479871426	28.878336204023537	21.590694603206245
20-21	22.578778323835827	26.808656468005378	29.010890160621944	21.601675047536848
22-23	22.22092950905168	27.06920353060214	28.79294309437686	21.916923865969316
24-25	22.102584163376406	27.253493721085587	28.96307398849406	21.68084812704395
26-27	22.11757836693378	27.36513341370468	28.863961558954642	21.6533266604069
28-29	21.579977008563507	27.18333212919833	29.131978866104298	22.10471199613387
30-31	22.248728216020883	26.902966742318867	29.213619332537842	21.63468570912241
32-33	21.840024725114194	27.434950100373417	28.90875714764111	21.816268026871274
34-35	21.843304920155372	27.479932199817327	29.209533177424696	21.4672297026026
36-37	22.199003145360095	27.23160291820395	28.72924649591015	21.840147440525804
38-39	21.878681460569254	27.179913440582876	28.96917006447115	21.972235034376723
40-41	22.060922556591912	27.06513033355146	28.949282447576035	21.924664662280595
42-43	22.185214375016994	27.09534746131292	28.78968486771049	21.929753295959596
44-45	22.034591772282425	27.423741122979038	28.495259523210663	22.046407581527873
46-47	22.451045688512842	27.141724839874797	27.90793285869168	22.49929661292068
48-49	22.19360240105333	27.290507140739724	28.06792465646597	22.447965801740974
50-51	22.10829020156271	27.609730125176817	28.006703380722385	22.275276292538088
52-53	22.258708774598592	27.66036241671316	28.071834605980822	22.00909420270743
54-55	22.2111258831067	27.626515280241104	27.806194980105055	22.35616385654714
56-57	22.480007491338142	27.929453728217656	27.40019920149146	22.190339578952745
58-59	22.098792774921154	28.109702293673504	27.883782144117752	21.90772278728759
60-61	22.442343926593324	28.303904782592582	27.33267972874918	21.921071562064913
62-63	22.11676236284225	28.53152014509806	27.317079326663958	22.03463816539573
64-65	22.15696639642808	28.3533896399518	27.34286210481448	22.146781858805646
66-67	22.349656629456433	28.16808453284056	27.279864036785533	22.20239480091747
68-69	22.34068701422502	27.946697278454707	27.413963709950046	22.29865199737023
70-71	22.37885605316251	27.615234742787592	27.510965441271985	22.49494376277791
72-73	22.43073388202067	27.715233210596992	27.433359987028844	22.42067292035349
74-75	22.288490104951507	27.829801157510232	27.44038477403754	22.44132396350072
76-77	22.403848631818498	27.772936064999886	27.341047551847975	22.482167751333645
78-79	22.897631169201	27.722023643112742	26.967952971347415	22.41239221633884
80-81	22.36928489208012	27.808690257654785	27.31119594717365	22.510828903091447
82-83	22.40816371984566	27.79323237463402	27.383361424041546	22.415242481478774
84-85	22.562062555589833	27.724124211375056	27.338675303747628	22.375137929287487
86-87	22.33681251625019	27.788631812033866	27.43295193952129	22.44160373219465
88-89	22.447452929492197	27.762063002217758	27.35089185175254	22.439592216537505
90-91	22.481137314860636	27.740236098017977	27.38911710670689	22.3895094804145
92-93	22.491504340094206	27.733185656092747	27.36201594946858	22.413294054344462
94-95	22.366501249772895	27.841411849620478	27.37938962964433	22.412697270962298
96-97	22.43568460148195	27.916818944061657	27.46298936258041	22.184507091875986
98-99	23.53433981586149	29.86141558797976	27.592979985107597	19.011264611051153
100	22.475969579522104	30.847267401981384	21.446208356417568	25.230554662078948
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1761.0
1	1588.0
2	1962.0
3	2663.5
4	3567.5
5	5506.5
6	7096.5
7	8214.5
8	9095.5
9	9364.0
10	9055.5
11	8731.0
12	9679.5
13	12533.0
14	19375.5
15	28854.0
16	36897.0
17	42140.5
18	43609.0
19	41380.5
20	37350.5
21	32903.0
22	29850.5
23	30064.5
24	33704.0
25	40741.0
26	50833.0
27	62460.0
28	74295.0
29	87533.5
30	100629.5
31	113432.5
32	127619.0
33	142292.0
34	157386.5
35	171292.0
36	186965.0
37	201455.5
38	210787.0
39	218917.0
40	229190.5
41	238897.0
42	245631.5
43	253118.0
44	259960.0
45	269734.0
46	281452.5
47	289714.5
48	287067.0
49	278471.5
50	271189.5
51	258093.5
52	242232.5
53	226989.5
54	212290.5
55	197235.5
56	181711.5
57	164581.5
58	145863.5
59	126990.0
60	106481.5
61	86334.5
62	68422.0
63	52944.0
64	40303.0
65	30030.5
66	22480.0
67	17226.5
68	13016.0
69	9360.0
70	6550.5
71	4570.0
72	3106.0
73	2038.0
74	1333.5
75	877.5
76	568.5
77	353.0
78	222.0
79	149.0
80	109.5
81	80.0
82	54.5
83	32.0
84	20.0
85	12.5
86	8.5
87	7.5
88	6.5
89	5.0
90	3.5
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	2.3360046844680604E-4
4	0.022067457585941613
5	0.015511071104867921
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008256128150063385
26-27	0.05306680308780075
28-29	0.0865213678311855
30-31	0.07610966534221958
32-33	0.03590766756162149
34-35	0.0041871509041344745
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.374730591038504E-4
56-57	7.150771636362151E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	9.15445934770083E-6
72-73	0.013135728377524159
74-75	0.02765998313374748
76-77	9.123195970293745E-4
78-79	5.029471003324568
80-81	0.0
82-83	0.012248340180462107
84-85	0.049015314206346854
86-87	0.0386744438031607
88-89	0.009755110810278654
90-91	2.0173451334574672E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	350.0
22-23	838.0
24-25	1918.0
26-27	2914.0
28-29	5047.0
30-31	12288.0
32-33	16053.0
34-35	22563.0
36-37	32684.0
38-39	39393.0
40-41	41248.0
42-43	48960.0
44-45	59556.0
46-47	58810.0
48-49	51263.0
50-51	44906.0
52-53	43860.0
54-55	50899.0
56-57	55582.0
58-59	56717.0
60-61	56232.0
62-63	58544.0
64-65	61590.0
66-67	66843.0
68-69	57238.0
70-71	50446.0
72-73	47131.0
74-75	49091.0
76-77	50366.0
78-79	50280.0
80-81	51184.0
82-83	49413.0
84-85	48994.0
86-87	51516.0
88-89	54943.0
90-91	57951.0
92-93	59271.0
94-95	70240.0
96-97	200540.0
98-99	383274.0
100-101	4200197.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.47156527426617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.10974505166789	79.69512011408717
2	6.498763076850418	11.369139573574245
3	1.4197330087507822	3.72558805640923
4	0.4623508769620698	1.6177021965520757
5	0.20769510175846195	0.9083707825305329
6	0.10122342926238756	0.5312503080005998
7	0.059304037007276225	0.3631191860876621
8	0.03866535506767364	0.2705695303723754
9	0.024410592163452628	0.1921709435228043
>10	0.07576364074018012	1.0201243141676646
>50	0.001469611859900823	0.08509536676649067
>100	7.729825147040641E-4	0.1418327621038077
>500	7.742659535089185E-5	0.04697941437112879
>1k	2.5808799421239578E-5	0.032937451454258596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.6720093689361214E-5	0.0	0.0	0.0
3	0.0	6.229345825248161E-5	0.0	0.0	0.0
4	0.0	7.786682281560202E-5	0.0	1.5573364563120402E-5	0.0
5	0.0	9.344018737872243E-5	0.0	1.5573364563120402E-5	0.0
6	1.5573364563120402E-5	9.344018737872243E-5	0.0	1.5573364563120402E-5	0.0
7	1.5573364563120402E-5	1.0901355194184282E-4	0.0	3.1146729126240805E-5	0.0
8	1.5573364563120402E-5	1.2458691650496322E-4	0.0	3.1146729126240805E-5	0.0
9	1.5573364563120402E-5	1.8688037475744486E-4	0.0	3.1146729126240805E-5	0.0
10-11	2.3360046844680604E-5	2.0245373932056525E-4	0.0	3.1146729126240805E-5	0.0
12-13	3.1146729126240805E-5	2.0245373932056525E-4	0.0	3.893341140780101E-5	0.0
14-15	3.1146729126240805E-5	2.803205621361673E-4	0.0	4.6720093689361214E-5	1.5573364563120402E-5
16-17	6.229345825248161E-5	2.803205621361673E-4	0.0	5.450677597092141E-5	3.1146729126240805E-5
18-19	6.229345825248161E-5	2.958939266992877E-4	0.0	6.229345825248161E-5	3.1146729126240805E-5
20-21	6.229345825248161E-5	3.270406558255285E-4	0.0	8.565350509716222E-5	3.1146729126240805E-5
22-23	6.229345825248161E-5	3.504007026702091E-4	0.0	1.4794696334964385E-4	3.893341140780101E-5
24-25	7.008014053404182E-5	4.204808432042509E-4	0.0	2.0245373932056525E-4	4.6720093689361214E-5
26-27	7.786682281560202E-5	4.360542077673713E-4	0.0	2.1802710388368565E-4	4.6720093689361214E-5
28-29	7.786682281560202E-5	4.438408900489315E-4	0.0	3.659740672333295E-4	4.6720093689361214E-5
30-31	8.565350509716222E-5	4.983476660198529E-4	0.0	9.188285092241039E-4	4.6720093689361214E-5
32-33	1.0122686966028263E-4	4.983476660198529E-4	0.0	0.002071257486895014	6.229345825248161E-5
34-35	1.0901355194184282E-4	5.372810774276539E-4	0.0	0.0036519539900517345	6.229345825248161E-5
36-37	1.0901355194184282E-4	5.917878533985753E-4	0.0	0.005902305169422632	6.229345825248161E-5
38-39	1.0901355194184282E-4	6.462946293694968E-4	0.0	0.009382952149280043	6.229345825248161E-5
40-41	1.0901355194184282E-4	6.852280407772977E-4	0.0	0.013470960347099149	6.229345825248161E-5
42-43	1.4016028106808364E-4	7.085880876219784E-4	0.0	0.017558968544918258	7.008014053404182E-5
44-45	1.4016028106808364E-4	8.020282750007008E-4	0.0	0.021896150575747286	8.565350509716222E-5
46-47	1.4016028106808364E-4	8.721084155347426E-4	0.0	0.026856267189101134	9.344018737872243E-5
48-49	1.4016028106808364E-4	8.954684623794232E-4	0.0	0.03183974384929967	1.0901355194184282E-4
50-51	1.4794696334964385E-4	0.0010278420611659466	0.0	0.03647281980682798	1.0901355194184282E-4
52-53	1.5573364563120404E-4	0.0010745621548553079	0.0	0.04077885510853078	1.1680023422340302E-4
54-55	1.5573364563120404E-4	0.001082348837136868	0.0	0.04524062405586478	1.2458691650496322E-4
56-57	1.5573364563120404E-4	0.0011057088839815487	0.0	0.050582288101015074	1.2458691650496322E-4
58-59	1.5573364563120404E-4	0.0011057088839815487	0.0	0.055542404714368915	1.3237359878652343E-4
60-61	1.5573364563120404E-4	0.0011290689308262295	0.0	0.06025334749471284	1.4016028106808364E-4
62-63	1.7130701019432443E-4	0.0011446422953893497	0.0	0.06547042462335817	1.5573364563120404E-4
64-65	1.7130701019432443E-4	0.001269229211894313	0.0	0.07029038095564394	1.5573364563120404E-4
66-67	1.7130701019432443E-4	0.0013237359878652344	0.0	0.07530500434496872	1.5573364563120404E-4
68-69	1.7130701019432443E-4	0.0013548827169914753	0.0	0.07940858590735095	1.5573364563120404E-4
70-71	1.8688037475744486E-4	0.0013782427638361558	0.0	0.08357446092798565	1.7130701019432443E-4
72-73	1.8688037475744486E-4	0.001393816128399276	0.0	0.08652561351269697	2.0245373932056525E-4
74-75	1.8688037475744486E-4	0.0014249628575255168	0.0	0.0888071114211941	2.0245373932056525E-4
76-77	1.8688037475744486E-4	0.0014716829512148783	0.0	0.09087058222580756	2.0245373932056525E-4
78-79	2.0245373932056525E-4	0.0015339764094673596	0.0	0.09266930583284796	2.0245373932056525E-4
80-81	2.0245373932056525E-4	0.0015573364563120403	0.0	0.0933856806027515	2.0245373932056525E-4
82-83	2.0245373932056525E-4	0.0015962698677198413	0.0	0.09381394812823732	2.5696051529148665E-4
84-85	2.0245373932056525E-4	0.0016040565500014016	0.0	0.09401640186755787	2.6474719757304686E-4
86-87	2.0245373932056525E-4	0.0016507766436907629	0.0	0.0940709086435288	2.6474719757304686E-4
88	2.1802710388368565E-4	0.0017597901956326056	0.0	0.09407869532581035	2.6474719757304686E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9885	0.0	50.91769	1
CTTATAC	6135	0.0	40.367184	1
GTATCAA	17985	0.0	36.78244	1
TATACAC	8695	0.0	31.781664	3
TCAACGC	21685	0.0	30.097406	4
ATCAACG	21715	0.0	30.017437	3
TTATACA	8800	0.0	29.388859	2
TATCAAC	22410	0.0	29.161812	2
CAACGCA	22360	0.0	29.144283	5
AACGCAG	22875	0.0	28.449266	6
ACGCAGA	26165	0.0	24.88816	7
CGCAGAG	26270	0.0	24.74052	8
ACACATC	11190	0.0	22.689903	6
GTGGTAT	4620	0.0	22.281929	1
TACACAT	12100	0.0	22.17014	5
TGGTATC	4480	0.0	21.659817	2
GCAGAGT	30000	0.0	21.566038	9
ACATCTC	11755	0.0	21.527563	8
GAGTACT	18085	0.0	20.452545	12-13
CACATCT	12725	0.0	19.555119	7
>>END_MODULE