FastQCFastQC Report
Fri 27 May 2016
SRR2138888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6588996
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150050.056.7201231
GTATCAA275400.037.6146241
CTTATAC58700.035.7728581
GTGGTAT51450.032.3142171
TGGTATC50050.031.5695692
TTGTGCG31100.031.1609027
ATCAACG337200.030.2926333
GTGCGCT32750.029.2132079
TCAACGC351150.029.0641694
TATACAC77050.028.8060443
AACGCAG358150.028.4863836
TATCAAC359900.028.450762
ACGCAGA360900.028.2807487
CAACGCA362400.028.1789195
TGTGCGC36300.026.4698728
TTATACA80200.026.2343182
TCGAAGG49800.025.72846894
CGCAGAG418550.024.385438
ACACATC87200.022.7000756
GCAGAGT459500.022.086599
Produced by FastQC (version 0.11.5)