##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138888_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6588996
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.891491207461655	33.0	33.0	33.0	33.0	33.0
2	32.94193364209054	33.0	33.0	33.0	33.0	33.0
3	33.601997633630376	33.0	33.0	33.0	33.0	37.0
4	36.76907483325229	37.0	37.0	37.0	37.0	37.0
5	36.78447035026277	37.0	37.0	37.0	37.0	37.0
6	36.79430599138321	37.0	37.0	37.0	37.0	37.0
7	36.78593703805557	37.0	37.0	37.0	37.0	37.0
8	36.7702989954767	37.0	37.0	37.0	37.0	37.0
9	36.78050388860458	37.0	37.0	37.0	37.0	37.0
10-11	36.76900464046419	37.0	37.0	37.0	37.0	37.0
12-13	37.014805669938184	37.0	37.0	37.0	37.0	38.5
14-15	39.03499964182707	40.0	40.0	40.0	37.0	40.0
16-17	38.98065251519351	40.0	40.0	40.0	37.0	40.0
18-19	38.98977052042527	40.0	40.0	40.0	37.0	40.0
20-21	39.05858199204667	40.0	40.0	40.0	37.0	40.0
22-23	39.09003042243458	40.0	40.0	40.0	37.0	40.0
24-25	39.0699760866633	40.0	40.0	40.0	37.0	40.0
26-27	38.7879181699773	40.0	40.0	40.0	37.0	40.0
28-29	38.81445523413934	40.0	38.5	40.0	37.0	40.0
30-31	38.801136374595146	40.0	37.0	40.0	37.0	40.0
32-33	38.776001184156684	40.0	37.0	40.0	37.0	40.0
34-35	38.74985649000814	40.0	37.0	40.0	37.0	40.0
36-37	38.71226489487952	40.0	37.0	40.0	37.0	40.0
38-39	38.65810527465662	40.0	37.0	40.0	37.0	40.0
40-41	38.61494589852951	40.0	37.0	40.0	37.0	40.0
42-43	38.5462421751153	40.0	37.0	40.0	37.0	40.0
44-45	38.45916785877421	40.0	37.0	40.0	37.0	40.0
46-47	38.365714892017664	40.0	37.0	40.0	37.0	40.0
48-49	38.2983631603871	40.0	37.0	40.0	37.0	40.0
50-51	38.218181562835035	40.0	37.0	40.0	37.0	40.0
52-53	38.146018996406866	40.0	37.0	40.0	37.0	40.0
54-55	38.05097576477055	40.0	37.0	40.0	37.0	40.0
56-57	37.91553012090563	40.0	37.0	40.0	35.0	40.0
58-59	37.769401050808035	40.0	37.0	40.0	33.0	40.0
60-61	37.601720575654966	37.0	37.0	40.0	33.0	40.0
62-63	37.4143887177094	37.0	37.0	40.0	33.0	40.0
64-65	37.20720836615729	37.0	37.0	40.0	33.0	40.0
66-67	36.98065754790857	37.0	37.0	40.0	33.0	40.0
68-69	36.72607851880437	37.0	37.0	40.0	33.0	40.0
70-71	36.49187606126542	37.0	37.0	38.5	33.0	40.0
72-73	36.26322944772109	37.0	37.0	37.0	33.0	40.0
74-75	35.80026353954635	37.0	35.0	37.0	33.0	40.0
76-77	33.938397028516796	35.0	33.0	37.0	27.0	37.0
78-79	31.597546062891865	37.0	33.0	37.0	10.5	37.0
80-81	34.50829205391137	37.0	33.0	37.0	30.0	37.0
82-83	35.47523824288989	37.0	35.0	37.0	33.0	37.0
84-85	35.59048634435499	37.0	37.0	37.0	33.0	37.0
86-87	35.57149182598703	37.0	37.0	37.0	33.0	37.0
88-89	35.49728403778333	37.0	35.0	37.0	33.0	37.0
90-91	35.43060152346141	37.0	33.0	37.0	33.0	37.0
92-93	35.36326192008411	37.0	33.0	37.0	33.0	37.0
94-95	35.32231351946126	37.0	33.0	37.0	33.0	37.0
96-97	35.29361183457644	37.0	33.0	37.0	33.0	37.0
98-99	35.2464164498238	37.0	33.0	37.0	33.0	37.0
100	35.18018674984135	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	1.0
18	5.0
19	2.0
20	25.0
21	97.0
22	371.0
23	1089.0
24	2684.0
25	5445.0
26	10072.0
27	16869.0
28	25823.0
29	36923.0
30	52204.0
31	72084.0
32	98410.0
33	139159.0
34	205327.0
35	336890.0
36	757422.0
37	2194230.0
38	2485779.0
39	148083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.48501687438979	0.0	24.01595148504799	29.49903164056222
2	15.92563683409266	23.404585335988482	38.56211927798632	22.107658551932545
3	16.587397049036266	25.15555811886876	32.830067259146155	25.426977572948818
4	13.173084673609065	23.52054310217761	36.514215947235705	26.792156276977618
5	17.27406493533697	35.98894318499537	31.81457203337991	14.922419846287745
6	27.21501120959855	37.39231288044491	19.964953689454358	15.427722220502183
7	26.34887014652915	32.893053812750836	21.91811924001775	18.83995680070226
8	24.428243696004674	31.69303790744447	21.742508266813335	22.13621012973752
9	24.867582253806194	19.887885802328608	22.719743645314097	32.5247882985511
10-11	25.23911837251077	25.696995414779426	27.543392043340138	21.52049416936966
12-13	25.934413983556826	24.0676500638337	27.552414662264173	22.445521290345297
14-15	23.292585091871356	26.044491452111973	26.202565610906426	24.46035784511024
16-17	21.473537091235144	28.77956672002836	27.169632520645028	22.577263668091465
18-19	21.930374521399013	27.02534650195568	29.476167537512545	21.56811143913276
20-21	22.950290211165182	26.254780254379273	29.434733639073325	21.360195895382226
22-23	22.90626086684629	25.994073105349457	29.29113593198096	21.808530095823293
24-25	22.300380915131598	26.41621689084097	29.547728568817067	21.735673625210364
26-27	22.274123870030525	26.647738237604717	29.34859510737844	21.729542784986318
28-29	22.011725076189723	26.445532361458206	29.40409608791731	22.138646474434758
30-31	22.192839727218274	26.79340580755934	29.281935502787565	21.731818962434822
32-33	22.044802909514573	26.764650387152656	29.480184091767438	21.71036261156533
34-35	22.19605031989866	26.44079232549555	29.356802269388833	22.00635508521696
36-37	22.005338842833954	26.92186139348987	29.225641442958043	21.84715832071813
38-39	22.025116452694785	26.687915617924894	29.303151075654494	21.98381685372583
40-41	22.225724079049012	26.518626994129807	29.173739878843225	22.08190904797796
42-43	22.33390744245825	26.76675048186507	28.792982245747634	22.106359829929048
44-45	22.249756300522588	27.15341847042066	28.364527638127175	22.23229759092958
46-47	22.523387433278767	26.915893613763487	27.797784121827334	22.762934831130416
48-49	22.384227650831413	27.362581671305968	27.62232031329263	22.63087036456999
50-51	22.34415601570214	27.605165067348423	27.35571663897456	22.694962277974874
52-53	22.615603983814513	27.264298453995107	27.466094782267962	22.65400277992242
54-55	22.56729085750359	27.311403197349804	27.393386958122505	22.7279189870241
56-57	22.663049431664568	27.690016850484195	26.820507333989678	22.82642638386156
58-59	22.558767879863947	27.556343907409158	27.273990504364487	22.61089770836241
60-61	22.661950242907196	27.786478747763553	26.982424988330934	22.569146020998314
62-63	22.49482002108151	28.024647118747524	26.97689735120983	22.503635508961136
64-65	22.62784739917626	27.779028420158795	26.949827320925678	22.643296859739266
66-67	22.67598782279603	27.726899425565378	26.893431464977034	22.70368128666155
68-69	22.890545295152073	27.541583869900133	26.793676245706806	22.77419458924099
70-71	22.821041752702705	27.168862224853406	27.095344363841278	22.914751658602608
72-73	22.63700187792131	27.362671640627127	26.989260812412713	23.01106566903885
74-75	22.59197747804517	27.57110369200374	27.112782213059024	22.72413661689207
76-77	22.970004999246985	27.276141755356388	26.684354089109775	23.069499156286856
78-79	23.022579439733523	27.36150111803101	26.642081649448706	22.973837792786757
80-81	22.79324892784847	27.5698813006992	26.730277785668484	22.906591985783848
82-83	22.8963111884502	27.12925279753985	26.93941241462246	23.03502359938749
84-85	22.736813225049655	27.3305574508488	27.048556479783198	22.884072844318347
86-87	22.77857984488094	27.248216794421932	26.965481396718932	23.007721963978195
88-89	23.026997881984826	27.045490721315378	26.884422007609615	23.04308938909018
90-91	22.82003654700203	26.93808568980078	27.168654153950193	23.073223609246998
92-93	22.91519849040849	27.10146907326977	26.93779517781315	23.045537258508595
94-95	22.906394515181532	27.036470177507482	26.984369618511455	23.072765688799535
96-97	23.037172495797286	27.272677573222904	27.110899722552517	22.579250208427297
98-99	24.057040657988598	29.246691966796146	26.650845528877603	20.045421846337653
100	23.481344367862413	29.372675840537088	21.08688800845836	26.059091783142136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1480.0
1	1427.0
2	2247.0
3	3319.5
4	4705.5
5	8126.0
6	10740.0
7	12276.0
8	13337.5
9	13204.5
10	12551.5
11	11642.0
12	12799.5
13	17121.0
14	28363.5
15	46084.5
16	62352.0
17	72633.0
18	74337.0
19	68159.0
20	58024.0
21	46471.5
22	36964.0
23	32642.0
24	33369.0
25	38762.0
26	48023.0
27	58720.0
28	69591.5
29	81867.5
30	94141.5
31	105299.0
32	117515.5
33	130875.0
34	145099.0
35	157755.0
36	171291.5
37	184002.5
38	192927.0
39	203142.5
40	215939.5
41	227723.0
42	236442.0
43	247547.0
44	261649.0
45	277090.5
46	292588.5
47	298984.0
48	295630.0
49	290611.0
50	286041.5
51	280134.0
52	270962.5
53	259497.0
54	248075.0
55	234223.5
56	215773.0
57	194067.0
58	170715.5
59	146759.0
60	123723.5
61	101610.0
62	81497.5
63	64790.5
64	49815.0
65	38026.0
66	28735.5
67	21351.5
68	16053.0
69	11663.0
70	8258.5
71	5783.5
72	3929.0
73	2567.5
74	1666.0
75	1110.0
76	739.0
77	496.5
78	352.0
79	236.5
80	152.0
81	92.5
82	61.0
83	45.0
84	32.5
85	18.0
86	8.5
87	4.5
88	2.5
89	2.5
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.580059238160108E-4
2	5.311886666800223E-4
3	0.004568222533448191
4	0.020625297086232865
5	0.012778881638416537
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	7.588608193860406E-6
22-23	5.540116170923663E-4
24-25	0.013640208937335118
26-27	0.057618208447309256
28-29	0.08556008000703326
30-31	0.07115680028797576
32-33	0.03195609775452711
34-35	0.003201181710867736
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	3.3426579562571425E-5
54-55	3.035801715599012E-4
56-57	5.196007456015158E-4
58-59	0.0
60-61	8.703386835953343E-6
62-63	0.0
64-65	8.894334593482659E-6
66-67	5.4000875354189495E-5
68-69	1.8229611250805634E-4
70-71	0.0015312143110610217
72-73	0.01780406076211639
74-75	0.06905990112160594
76-77	0.9619445209276686
78-79	4.088419914735129
80-81	0.0011518669175914385
82-83	0.01756320159582134
84-85	0.05109680240747587
86-87	0.037100941154509695
88-89	0.007497199304440165
90-91	2.0770058216396174E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	415.0
22-23	880.0
24-25	2092.0
26-27	3762.0
28-29	8049.0
30-31	16037.0
32-33	21859.0
34-35	31133.0
36-37	44020.0
38-39	52416.0
40-41	54990.0
42-43	69204.0
44-45	84807.0
46-47	80650.0
48-49	66705.0
50-51	55477.0
52-53	53248.0
54-55	57541.0
56-57	62204.0
58-59	62622.0
60-61	61766.0
62-63	62194.0
64-65	63378.0
66-67	71190.0
68-69	66345.0
70-71	60259.0
72-73	57113.0
74-75	57894.0
76-77	60589.0
78-79	62509.0
80-81	60720.0
82-83	59228.0
84-85	58147.0
86-87	61242.0
88-89	66492.0
90-91	67667.0
92-93	68398.0
94-95	83145.0
96-97	282870.0
98-99	1074173.0
100-101	3255478.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.51805296184061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.05748123394896	79.69173465204553
2	6.613846118980176	11.57661869844742
3	1.3538731616367572	3.554650291912209
4	0.458981742856727	1.6067675391941179
5	0.20521197126775576	0.8979876084907582
6	0.10654416563147791	0.5594722758306481
7	0.058899731796285146	0.3608352892749843
8	0.03402168043984506	0.23820089844681414
9	0.024789325884828416	0.1952562182109066
>10	0.08446331318914262	1.1125366333691364
>50	0.0012839523538297226	0.07562802602023236
>100	5.529931612438873E-4	0.08916471194005425
>500	2.5304431222746283E-5	0.014527740260605451
>1k	2.530442175719715E-5	0.02661941655665189
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0353638096001272E-5	0.0	0.0	0.0
3	0.0	3.0353638096001272E-5	0.0	0.0	0.0
4	0.0	3.0353638096001272E-5	0.0	0.0	0.0
5	0.0	3.0353638096001272E-5	0.0	0.0	0.0
6	0.0	3.0353638096001272E-5	0.0	0.0	1.5176819048000636E-5
7	0.0	4.5530457144001906E-5	0.0	1.5176819048000636E-5	1.5176819048000636E-5
8	0.0	7.588409524000319E-5	0.0	3.0353638096001272E-5	1.5176819048000636E-5
9	0.0	1.8212182857600762E-4	0.0	3.0353638096001272E-5	1.5176819048000636E-5
10-11	0.0	1.9729864762400826E-4	0.0	4.5530457144001906E-5	1.5176819048000636E-5
12-13	0.0	1.9729864762400826E-4	0.0	4.5530457144001906E-5	2.2765228572000953E-5
14-15	0.0	3.0353638096001275E-4	0.0	8.34725047640035E-5	5.3118866668002225E-5
16-17	0.0	3.3389001905601397E-4	0.0	9.106091428800381E-5	6.0707276192002543E-5
18-19	0.0	3.490668381040146E-4	0.0	9.106091428800381E-5	9.106091428800381E-5
20-21	0.0	3.490668381040146E-4	0.0	9.106091428800381E-5	9.106091428800381E-5
22-23	0.0	3.945972952480165E-4	0.0	1.4417978095600606E-4	9.106091428800381E-5
24-25	1.5176819048000636E-5	4.173625238200175E-4	0.0	1.8971023810000794E-4	9.106091428800381E-5
26-27	1.5176819048000636E-5	4.4012775239201844E-4	0.0	2.8077115238801174E-4	9.106091428800381E-5
28-29	2.2765228572000953E-5	4.4771616191601875E-4	0.0	6.601916285880276E-4	9.864932381200413E-5
30-31	3.0353638096001272E-5	4.8565820953602035E-4	0.0	0.0016998037333760714	1.0623773333600445E-4
32-33	3.0353638096001272E-5	4.8565820953602035E-4	0.0	0.00358172929532815	1.0623773333600445E-4
34-35	3.0353638096001272E-5	5.160118476320217E-4	0.0	0.006328733543016265	1.0623773333600445E-4
36-37	3.0353638096001272E-5	5.46365485728023E-4	0.0	0.010692069019316448	1.3659137143200574E-4
38-39	3.0353638096001272E-5	5.843075333480245E-4	0.0	0.01723327802900472	1.3659137143200574E-4
40-41	3.0353638096001272E-5	7.057220857320295E-4	0.0	0.023691014533928995	1.3659137143200574E-4
42-43	3.0353638096001272E-5	8.043714095440337E-4	0.0	0.029458205772169236	1.3659137143200574E-4
44-45	3.0353638096001272E-5	8.954323238320375E-4	0.0	0.03662925277234953	1.3659137143200574E-4
46-47	3.0353638096001272E-5	8.954323238320375E-4	0.0	0.04417213183920585	1.4417978095600606E-4
48-49	3.0353638096001272E-5	9.106091428800381E-4	0.0	0.05214755024893018	1.5176819048000638E-4
50-51	3.0353638096001272E-5	0.001001670057168042	0.0	0.05984978591579051	1.5176819048000638E-4
52-53	3.0353638096001272E-5	0.001001670057168042	0.0	0.06736989975407481	1.593566000040067E-4
54-55	3.0353638096001272E-5	0.0010092584666920425	0.0	0.07490519041140714	1.6694500952800699E-4
56-57	3.0353638096001272E-5	0.0010168468762160427	0.0	0.08076344256393539	1.8212182857600762E-4
58-59	3.0353638096001272E-5	0.001024435285740043	0.0	0.08650028016407962	1.8212182857600762E-4
60-61	3.0353638096001272E-5	0.001047200514312044	0.0	0.0928441905261439	1.8212182857600762E-4
62-63	3.0353638096001272E-5	0.0010775541524080453	0.0	0.09884662245962814	1.8212182857600762E-4
64-65	3.0353638096001272E-5	0.0011989687047920504	0.0	0.10428751208833636	1.9729864762400826E-4
66-67	3.0353638096001272E-5	0.001244499161936052	0.0	0.11022923674562862	1.9729864762400826E-4
68-69	3.0353638096001272E-5	0.001244499161936052	0.0	0.11501752315527282	1.9729864762400826E-4
70-71	3.0353638096001272E-5	0.001244499161936052	0.0	0.11878137427917698	1.9729864762400826E-4
72-73	3.0353638096001272E-5	0.0012520875714600523	0.0	0.1218319149078251	2.4282910476801017E-4
74-75	3.0353638096001272E-5	0.0012976180286040544	0.0	0.12433609005074522	2.4282910476801017E-4
76-77	3.0353638096001272E-5	0.001343148485748056	0.0	0.12694650292700133	2.504175142920105E-4
78-79	3.0353638096001272E-5	0.0013507368952720565	0.0	0.12843383119370538	2.5800592381601084E-4
80-81	3.0353638096001272E-5	0.0013507368952720565	0.0	0.12898778508895742	2.807711523880118E-4
82-83	3.0353638096001272E-5	0.0013886789428920582	0.0	0.12955691580325743	3.0353638096001275E-4
84-85	3.0353638096001272E-5	0.0014266209905120597	0.0	0.12974662604135745	3.0353638096001275E-4
86-87	3.0353638096001272E-5	0.0014721514476560616	0.0	0.12979974490802543	3.0353638096001275E-4
88	3.0353638096001272E-5	0.0015328587238480642	0.0	0.12980733331754943	3.0353638096001275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15005	0.0	56.720123	1
GTATCAA	27540	0.0	37.614624	1
CTTATAC	5870	0.0	35.772858	1
GTGGTAT	5145	0.0	32.314217	1
TGGTATC	5005	0.0	31.569569	2
TTGTGCG	3110	0.0	31.160902	7
ATCAACG	33720	0.0	30.292633	3
GTGCGCT	3275	0.0	29.213207	9
TCAACGC	35115	0.0	29.064169	4
TATACAC	7705	0.0	28.806044	3
AACGCAG	35815	0.0	28.486383	6
TATCAAC	35990	0.0	28.45076	2
ACGCAGA	36090	0.0	28.280748	7
CAACGCA	36240	0.0	28.178919	5
TGTGCGC	3630	0.0	26.469872	8
TTATACA	8020	0.0	26.234318	2
TCGAAGG	4980	0.0	25.728468	94
CGCAGAG	41855	0.0	24.38543	8
ACACATC	8720	0.0	22.700075	6
GCAGAGT	45950	0.0	22.08659	9
>>END_MODULE