FastQCFastQC Report
Fri 27 May 2016
SRR2138887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6265972
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81000.051.8340261
GTATCAA145500.036.5510061
TCAACGC173700.029.9721854
ATCAACG174850.029.7752933
CAACGCA178500.029.159465
TATCAAC183550.028.5985972
AACGCAG183450.028.4603926
ACGCAGA210450.024.788577
CGCAGAG212850.024.4888448
TGGTATC36050.021.2622932
GTGGTAT38100.021.1354751
GCAGAGT251300.020.7590719
GAGTACT153650.020.21164112-13
GTACATG175850.019.1985781
TACATGG172200.019.080372
CAGAGTA246100.019.02896310-11
AGAGTAC230500.018.26273210-11
TATACCG3351.4006218E-1017.99185
ACATGGG177650.017.9132583
GTACTTT172800.017.79774314-15
Produced by FastQC (version 0.11.5)