##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6265972
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.845838442942295	33.0	33.0	33.0	33.0	33.0
2	32.84195795959509	33.0	33.0	33.0	33.0	33.0
3	32.84206009857689	33.0	33.0	33.0	33.0	33.0
4	36.791490769508705	37.0	37.0	37.0	37.0	37.0
5	36.802419959744476	37.0	37.0	37.0	37.0	37.0
6	36.81381212683363	37.0	37.0	37.0	37.0	37.0
7	36.80028381869565	37.0	37.0	37.0	37.0	37.0
8	36.79738674223249	37.0	37.0	37.0	37.0	37.0
9	36.796767045878916	37.0	37.0	37.0	37.0	37.0
10-11	36.777584946118495	37.0	37.0	37.0	37.0	37.0
12-13	36.76618416105275	37.0	37.0	37.0	37.0	37.0
14-15	39.15340126001202	40.0	40.0	40.0	37.0	40.0
16-17	39.117323856538135	40.0	40.0	40.0	37.0	40.0
18-19	39.0892223903969	40.0	40.0	40.0	37.0	40.0
20-21	39.13877642138682	40.0	40.0	40.0	37.0	40.0
22-23	39.17192266024834	40.0	40.0	40.0	37.0	40.0
24-25	39.17803060264893	40.0	40.0	40.0	37.0	40.0
26-27	38.91591346917133	40.0	40.0	40.0	37.0	40.0
28-29	38.92412606056067	40.0	40.0	40.0	37.0	40.0
30-31	38.948777334034325	40.0	40.0	40.0	37.0	40.0
32-33	38.94063038551134	40.0	40.0	40.0	37.0	40.0
34-35	38.92192377283382	40.0	40.0	40.0	37.0	40.0
36-37	38.89271895650087	40.0	40.0	40.0	37.0	40.0
38-39	38.85344034492236	40.0	40.0	40.0	37.0	40.0
40-41	38.812550131824636	40.0	40.0	40.0	37.0	40.0
42-43	38.76025920383139	40.0	37.0	40.0	37.0	40.0
44-45	38.69650842365347	40.0	37.0	40.0	37.0	40.0
46-47	38.62942758208529	40.0	37.0	40.0	37.0	40.0
48-49	38.57984380574324	40.0	37.0	40.0	37.0	40.0
50-51	38.527994938033764	40.0	37.0	40.0	37.0	40.0
52-53	38.46414161167792	40.0	37.0	40.0	37.0	40.0
54-55	38.39527278160319	40.0	37.0	40.0	37.0	40.0
56-57	38.293244004347926	40.0	37.0	40.0	37.0	40.0
58-59	38.16632527016577	40.0	37.0	40.0	37.0	40.0
60-61	38.01643512176596	40.0	37.0	40.0	37.0	40.0
62-63	37.835036067092915	40.0	37.0	40.0	35.0	40.0
64-65	37.62404225186836	37.0	37.0	40.0	33.0	40.0
66-67	37.40015922313665	37.0	37.0	40.0	33.0	40.0
68-69	37.14620208837033	37.0	37.0	40.0	33.0	40.0
70-71	36.89469789408102	37.0	37.0	40.0	33.0	40.0
72-73	36.648866099419905	37.0	37.0	38.5	33.0	40.0
74-75	36.37925857871092	37.0	37.0	37.0	33.0	40.0
76-77	34.686687534054045	37.0	33.0	37.0	30.0	37.0
78-79	31.327281928732077	37.0	33.0	37.0	8.5	37.0
80-81	34.54906844891403	37.0	33.0	37.0	30.0	37.0
82-83	35.62642102421698	37.0	35.0	37.0	33.0	37.0
84-85	35.75158647156057	37.0	37.0	37.0	33.0	37.0
86-87	35.71415887755714	37.0	37.0	37.0	33.0	37.0
88-89	35.63629899148402	37.0	37.0	37.0	33.0	37.0
90-91	35.57082474815577	37.0	37.0	37.0	33.0	37.0
92-93	35.50200300756258	37.0	33.0	37.0	33.0	37.0
94-95	35.45309557467098	37.0	33.0	37.0	33.0	37.0
96-97	35.424751000612034	37.0	33.0	37.0	33.0	37.0
98-99	35.38706885333348	37.0	33.0	37.0	33.0	37.0
100	35.337311703897285	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	19.0
21	79.0
22	309.0
23	924.0
24	2287.0
25	5080.0
26	9179.0
27	15084.0
28	23108.0
29	33226.0
30	45783.0
31	61937.0
32	83515.0
33	115062.0
34	168131.0
35	274828.0
36	611412.0
37	1978619.0
38	2711523.0
39	125864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53255073594328	20.281434388790757	14.429700611493319	23.756314263772644
2	14.28613150521579	21.120649757132654	42.01573195667009	22.577486780981467
3	17.79048560187783	26.37425159065911	30.865202372949952	24.970060434513105
4	12.197376247154185	18.41570752604134	39.442189927882076	29.944726298922397
5	13.162897092599247	37.7361773283492	35.822560556416875	13.278365022634683
6	29.254615245647443	38.335999586337124	19.116236076382084	13.29314909163335
7	25.898408100131952	33.03801868249651	23.395109330204473	17.668463887167064
8	24.070343755126896	36.5841245380605	21.594351203612145	17.751180503200462
9	25.413886305269155	16.947250961223574	21.50419759296722	36.134665140540044
10-11	23.73926982118656	26.945923154460317	29.295031959925772	20.019775064427357
12-13	24.7071005105034	24.544571855731242	29.298151986635112	21.450175647130244
14-15	21.92604754697276	25.974924560786416	26.967667586130297	25.13136030611053
16-17	21.40556963867697	29.143259816673293	27.582145276104008	21.869025268545723
18-19	21.435995883799034	28.209286603898004	29.352748464244655	21.001969048058307
20-21	22.219308729177158	27.385251087818595	29.32442453480688	21.071015648197363
22-23	21.8779272437141	27.42534386360339	29.171351719649195	21.525377173033313
24-25	21.72178317377179	27.536959877879724	29.247036452892594	21.49422049545589
26-27	21.764753855391287	27.67679181729758	29.083077974009225	21.475376353301908
28-29	21.573228532004972	27.694085677100276	29.168969440637348	21.563716350257398
30-31	21.957613638708725	27.563596899234728	29.0750415975241	21.40374786453245
32-33	21.72595983768265	27.69463276252576	29.055807758454055	21.523599641337533
34-35	21.793886707211065	27.7453649661557	29.041886342757422	21.418861983875814
36-37	21.85326528378388	27.62371619931058	28.95900322674795	21.564015290157595
38-39	21.642196148107388	27.73779416734441	29.032428381894128	21.58758130265408
40-41	21.889799818866436	27.60205955090294	28.914053356550262	21.59408727368036
42-43	21.83190794866316	27.68885840778591	28.765390604257068	21.713843039293856
44-45	21.867688788991654	27.85846221660102	28.539759490107276	21.734089504300048
46-47	21.98835496426114	27.828035818719854	28.16699650572868	22.016612711290318
48-49	21.941300108427168	27.912038846253672	28.179921646987093	21.966739398332066
50-51	21.925553596449472	28.039241155529933	28.08427638591857	21.950928862102025
52-53	22.034585644416055	27.99046304827132	28.038471287593786	21.936480019718843
54-55	22.04678641830369	27.909914147907955	28.02342910856507	22.019870325223284
56-57	22.072609757574327	28.019510073230048	27.92317065599964	21.98470951319598
58-59	21.90597064568626	28.05426526222281	28.054727934270414	21.98503615782052
60-61	22.058434296710942	28.10852687372399	27.941725251916505	21.891313577648567
62-63	21.921618584624134	28.20356234008314	27.898921993089758	21.975897082202966
64-65	21.92312905046004	28.19079502564637	27.848300955901788	22.0377749679918
66-67	21.97149768388576	28.242345998274924	27.711872159774874	22.07428415806444
68-69	21.97834438877052	28.192988858600305	27.75935872324139	22.069308029387788
70-71	21.977007920826203	27.95154345541017	27.812945151581292	22.258503472182333
72-73	22.02824975997288	27.931111469689974	27.731777418816804	22.30886135152034
74-75	21.935880997467546	28.09638840090347	27.655385457781023	22.31234514384796
76-77	22.1284596378498	27.988602246866783	27.551612310765904	22.331325804517512
78-79	22.632621315511287	27.954000139693935	27.133254199561108	22.28012434523367
80-81	22.124347934101227	28.060121866978278	27.473578719761594	22.341951479158904
82-83	22.16254369517715	28.0506821506206	27.529422443601664	22.257351710600588
84-85	22.247555355308727	28.004182910205845	27.522460948686327	22.225800785799105
86-87	22.097599341887904	28.080846504198615	27.55092867860468	22.2706254753088
88-89	22.195018315203647	28.100271592572806	27.488470718685537	22.21623937353801
90-91	22.245117362479842	28.116840676507305	27.540919668052044	22.097122292960808
92-93	22.28877101508547	28.09079248937087	27.51998021969191	22.10045627585175
94-95	22.17000732277215	28.177841974113853	27.584237302521508	22.067913400592488
96-97	22.21651599565471	28.22718010687874	27.724384613509446	21.831919283957106
98-99	23.348332880339264	30.064417417335964	27.923923567076425	18.663326135248344
100	22.292381971763113	30.971309025702332	21.86926887131227	24.867040131222286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1152.0
1	1093.5
2	1524.0
3	2183.0
4	3022.0
5	4861.5
6	6395.0
7	7381.5
8	8029.5
9	8020.0
10	7614.0
11	7300.5
12	8237.5
13	10974.5
14	16816.5
15	24656.5
16	31305.5
17	35795.5
18	37108.0
19	35161.5
20	31797.0
21	28182.0
22	26145.0
23	27118.0
24	31283.0
25	38984.0
26	50119.0
27	62933.0
28	76096.5
29	90531.0
30	104505.0
31	118413.5
32	133482.0
33	148587.0
34	164137.5
35	178248.5
36	192254.5
37	205466.5
38	215049.0
39	223785.5
40	233613.0
41	242456.5
42	248523.5
43	253139.5
44	256479.0
45	259130.5
46	261255.5
47	262072.5
48	261714.5
49	255858.0
50	246487.0
51	236392.5
52	222948.5
53	206452.5
54	189560.5
55	172940.5
56	156418.0
57	139994.5
58	123230.5
59	106477.5
60	88264.5
61	70809.5
62	55650.0
63	42622.0
64	31771.5
65	23166.5
66	17233.0
67	13105.0
68	9766.0
69	6939.5
70	4882.5
71	3499.5
72	2413.0
73	1589.0
74	1033.0
75	703.5
76	460.5
77	281.0
78	171.0
79	116.5
80	89.5
81	64.0
82	47.5
83	33.0
84	20.0
85	13.5
86	11.0
87	10.0
88	7.5
89	4.5
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	2.553474544731448E-4
4	0.02409841601590304
5	0.016422033165804126
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008739673616689248
26-27	0.052124348625271204
28-29	0.08455488803845039
30-31	0.07471649215500811
32-33	0.035563730757824444
34-35	0.004234669334080603
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.3180711531764625E-4
56-57	8.941525996592683E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.013835448826846234
74-75	0.029094222675630253
76-77	0.001104171597099562
78-79	5.228848703905389
80-81	0.0
82-83	0.012630648268022356
84-85	0.04905972673484143
86-87	0.038323150746651324
88-89	0.009923581611291947
90-91	3.9336836016335014E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	258.0
22-23	758.0
24-25	1473.0
26-27	2265.0
28-29	3531.0
30-31	8550.0
32-33	11341.0
34-35	15687.0
36-37	22536.0
38-39	26430.0
40-41	30891.0
42-43	37705.0
44-45	45106.0
46-47	44627.0
48-49	39303.0
50-51	32834.0
52-53	29143.0
54-55	33033.0
56-57	35689.0
58-59	36382.0
60-61	39775.0
62-63	42964.0
64-65	45700.0
66-67	49099.0
68-69	48050.0
70-71	45870.0
72-73	45096.0
74-75	46323.0
76-77	47985.0
78-79	47746.0
80-81	49058.0
82-83	48083.0
84-85	48699.0
86-87	51159.0
88-89	54431.0
90-91	56527.0
92-93	59394.0
94-95	70521.0
96-97	204165.0
98-99	391379.0
100-101	4316340.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.06647282961883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.58843530842314	78.87215541458507
2	6.896547357677915	12.009161062708875
3	1.500869373208261	3.9202620750973227
4	0.5018029823539754	1.747608629157764
5	0.20640032405538725	0.8985274103196448
6	0.1081413913670275	0.5649293707928684
7	0.06058743850784916	0.36925941980618954
8	0.03554276510553727	0.2475666553880628
9	0.024174766079851143	0.18943304526483754
>10	0.0760696770381448	0.9982941809231699
>50	8.585112905952404E-4	0.04846874854137345
>100	5.170440725630619E-4	0.09187785667599478
>500	2.6530411137475422E-5	0.015845977938352997
>1k	2.6530408574004428E-5	0.026610152800518636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.595921590457155E-5	0.0	0.0	0.0
5	0.0	1.595921590457155E-5	0.0	0.0	0.0
6	0.0	1.595921590457155E-5	0.0	0.0	0.0
7	0.0	1.595921590457155E-5	0.0	0.0	0.0
8	0.0	1.595921590457155E-5	0.0	0.0	0.0
9	0.0	6.38368636182862E-5	0.0	1.595921590457155E-5	0.0
10-11	0.0	7.979607952285775E-5	0.0	1.595921590457155E-5	0.0
12-13	0.0	7.979607952285775E-5	0.0	1.595921590457155E-5	0.0
14-15	0.0	1.0373490337971508E-4	0.0	1.595921590457155E-5	7.979607952285774E-6
16-17	0.0	1.1171451133200085E-4	0.0	3.19184318091431E-5	1.595921590457155E-5
18-19	0.0	1.1171451133200085E-4	0.0	3.19184318091431E-5	1.595921590457155E-5
20-21	0.0	1.276737272365724E-4	0.0	3.9898039761428874E-5	1.595921590457155E-5
22-23	1.595921590457155E-5	1.6757176699800128E-4	0.0	7.181647157057197E-5	1.595921590457155E-5
24-25	1.595921590457155E-5	1.835309829025728E-4	0.0	9.57552954274293E-5	1.595921590457155E-5
26-27	1.595921590457155E-5	1.915105908548586E-4	0.0	1.1969411928428663E-4	1.595921590457155E-5
28-29	4.787764771371465E-5	1.915105908548586E-4	0.0	3.830211817097172E-4	3.19184318091431E-5
30-31	4.787764771371465E-5	2.0746980675943015E-4	0.0	0.0011011858974154369	3.19184318091431E-5
32-33	4.787764771371465E-5	2.0746980675943015E-4	0.0	0.0026651890560634487	3.19184318091431E-5
34-35	4.787764771371465E-5	2.2342902266400168E-4	0.0	0.004923418106560323	3.19184318091431E-5
36-37	4.787764771371465E-5	2.3938823856857323E-4	0.0	0.009096753065605784	4.787764771371465E-5
38-39	4.787764771371465E-5	2.3938823856857323E-4	0.0	0.02709874860596249	4.787764771371465E-5
40-41	4.787764771371465E-5	2.553474544731448E-4	0.0	0.045667296310931485	5.585725566600042E-5
42-43	4.787764771371465E-5	2.7130667037771636E-4	0.0	0.05372670034274012	6.38368636182862E-5
44-45	4.787764771371465E-5	3.431231419482883E-4	0.0	0.06378898597057249	6.38368636182862E-5
46-47	5.585725566600042E-5	4.069600055665745E-4	0.0	0.07615737829661542	7.181647157057197E-5
48-49	6.38368636182862E-5	4.149396135188603E-4	0.0	0.08894868984412953	7.979607952285775E-5
50-51	6.38368636182862E-5	4.3887843737571764E-4	0.0	0.10157242962464563	7.979607952285775E-5
52-53	6.38368636182862E-5	4.468580453280034E-4	0.0	0.17285426746241445	7.979607952285775E-5
54-55	6.38368636182862E-5	4.5483765328028917E-4	0.0	0.27129071116181175	7.979607952285775E-5
56-57	6.38368636182862E-5	4.6281726123257493E-4	0.0	0.37175397528108967	8.777568747514352E-5
58-59	6.38368636182862E-5	4.6281726123257493E-4	0.0	0.47354185432044704	9.57552954274293E-5
60-61	6.38368636182862E-5	4.6281726123257493E-4	0.0	0.5526277487355513	9.57552954274293E-5
62-63	7.979607952285775E-5	4.7877647713714646E-4	0.0	0.603633402766562	9.57552954274293E-5
64-65	7.979607952285775E-5	5.904909884691473E-4	0.0	0.6106793965884303	9.57552954274293E-5
66-67	7.979607952285775E-5	6.224094202782905E-4	0.0	0.6175578186433006	9.57552954274293E-5
68-69	7.979607952285775E-5	6.623074600397192E-4	0.0	0.623039809306521	9.57552954274293E-5
70-71	7.979607952285775E-5	6.782666759442908E-4	0.0	0.626917898771332	9.57552954274293E-5
72-73	7.979607952285775E-5	6.862462838965767E-4	0.0	0.6302773137192441	9.57552954274293E-5
74-75	7.979607952285775E-5	6.862462838965767E-4	0.0	0.6327190737526436	1.1171451133200085E-4
76-77	7.979607952285775E-5	6.862462838965767E-4	0.0	0.6351767930019476	1.1171451133200085E-4
78-79	7.979607952285775E-5	6.862462838965767E-4	0.0	0.6368525106719277	1.1171451133200085E-4
80-81	7.979607952285775E-5	6.862462838965767E-4	0.0	0.6375626957796812	1.1171451133200085E-4
82-83	7.979607952285775E-5	6.862462838965767E-4	0.0	0.6380813702965797	1.1171451133200085E-4
84-85	8.777568747514352E-5	7.022054998011482E-4	0.0	0.6383047993192437	1.1171451133200085E-4
86-87	9.57552954274293E-5	7.341239316102912E-4	0.0	0.6383207585351482	1.1969411928428663E-4
88	9.57552954274293E-5	7.660423634194344E-4	0.0	0.6383207585351482	1.276737272365724E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8100	0.0	51.834026	1
GTATCAA	14550	0.0	36.551006	1
TCAACGC	17370	0.0	29.972185	4
ATCAACG	17485	0.0	29.775293	3
CAACGCA	17850	0.0	29.15946	5
TATCAAC	18355	0.0	28.598597	2
AACGCAG	18345	0.0	28.460392	6
ACGCAGA	21045	0.0	24.78857	7
CGCAGAG	21285	0.0	24.488844	8
TGGTATC	3605	0.0	21.262293	2
GTGGTAT	3810	0.0	21.135475	1
GCAGAGT	25130	0.0	20.759071	9
GAGTACT	15365	0.0	20.211641	12-13
GTACATG	17585	0.0	19.198578	1
TACATGG	17220	0.0	19.08037	2
CAGAGTA	24610	0.0	19.028963	10-11
AGAGTAC	23050	0.0	18.262732	10-11
TATACCG	335	1.4006218E-10	17.9918	5
ACATGGG	17765	0.0	17.913258	3
GTACTTT	17280	0.0	17.797743	14-15
>>END_MODULE