FastQCFastQC Report
Fri 27 May 2016
SRR2138886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553826
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96750.045.4029351
GTATCAA171650.036.3270651
TCAACGC203450.030.080264
ATCAACG204700.029.9384463
CAACGCA207450.029.4735285
TATCAAC213850.028.9380342
AACGCAG212850.028.741536
ACGCAGA239500.025.525477
CGCAGAG242750.025.1307878
GCAGAGT282700.021.5187919
GAGTACT172500.020.05435812-13
AGAGTAC250150.019.77191510-11
TACATGG179750.019.3358382
GTACATG185550.019.3319451
CAGAGTA271250.019.14203810-11
ACATGGG185200.018.2346023
GTACTTT190600.018.1618614-15
AGTACTT187350.017.82451612-13
CATGGGG132550.016.1982864
TGGTATC48050.015.87587452
Produced by FastQC (version 0.11.5)