##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6553826
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.847847501596775	33.0	33.0	33.0	33.0	33.0
2	32.84292304983379	33.0	33.0	33.0	33.0	33.0
3	32.84317374309297	33.0	33.0	33.0	33.0	33.0
4	36.7937735911817	37.0	37.0	37.0	37.0	37.0
5	36.8042264167526	37.0	37.0	37.0	37.0	37.0
6	36.8152518849295	37.0	37.0	37.0	37.0	37.0
7	36.80320792770513	37.0	37.0	37.0	37.0	37.0
8	36.80065369449845	37.0	37.0	37.0	37.0	37.0
9	36.79887519137676	37.0	37.0	37.0	37.0	37.0
10-11	36.78027826799186	37.0	37.0	37.0	37.0	37.0
12-13	36.76878505776625	37.0	37.0	37.0	37.0	37.0
14-15	39.157781805620104	40.0	40.0	40.0	37.0	40.0
16-17	39.12243596335942	40.0	40.0	40.0	37.0	40.0
18-19	39.09823841829185	40.0	40.0	40.0	37.0	40.0
20-21	39.14577124123986	40.0	40.0	40.0	37.0	40.0
22-23	39.17804779743582	40.0	40.0	40.0	37.0	40.0
24-25	39.18122107322219	40.0	40.0	40.0	37.0	40.0
26-27	38.92563297140711	40.0	40.0	40.0	37.0	40.0
28-29	38.933494982597196	40.0	40.0	40.0	37.0	40.0
30-31	38.95275743476451	40.0	40.0	40.0	37.0	40.0
32-33	38.944577156485295	40.0	40.0	40.0	37.0	40.0
34-35	38.92710519985937	40.0	40.0	40.0	37.0	40.0
36-37	38.896730559202496	40.0	40.0	40.0	37.0	40.0
38-39	38.86255476719711	40.0	40.0	40.0	37.0	40.0
40-41	38.81682534663659	40.0	40.0	40.0	37.0	40.0
42-43	38.75971726377104	40.0	37.0	40.0	37.0	40.0
44-45	38.6982770722784	40.0	37.0	40.0	37.0	40.0
46-47	38.630937298931464	40.0	37.0	40.0	37.0	40.0
48-49	38.57965400737873	40.0	37.0	40.0	37.0	40.0
50-51	38.536456267514794	40.0	37.0	40.0	37.0	40.0
52-53	38.4701895299153	40.0	37.0	40.0	37.0	40.0
54-55	38.39955002797652	40.0	37.0	40.0	37.0	40.0
56-57	38.3017105550098	40.0	37.0	40.0	37.0	40.0
58-59	38.17203721779799	40.0	37.0	40.0	37.0	40.0
60-61	38.0199500576621	40.0	37.0	40.0	37.0	40.0
62-63	37.83710255142903	40.0	37.0	40.0	35.0	40.0
64-65	37.62346495124072	37.0	37.0	40.0	33.0	40.0
66-67	37.39679571936041	37.0	37.0	40.0	33.0	40.0
68-69	37.14076553035344	37.0	37.0	40.0	33.0	40.0
70-71	36.88857887496007	37.0	37.0	40.0	33.0	40.0
72-73	36.643442193750225	37.0	37.0	38.5	33.0	40.0
74-75	36.37605254596036	37.0	37.0	37.0	33.0	40.0
76-77	34.678298005248095	37.0	33.0	37.0	30.0	37.0
78-79	31.426561168420236	37.0	33.0	37.0	8.5	37.0
80-81	34.58104779563301	37.0	33.0	37.0	30.0	37.0
82-83	35.63549533815421	37.0	35.0	37.0	33.0	37.0
84-85	35.75699986465888	37.0	37.0	37.0	33.0	37.0
86-87	35.720917504635295	37.0	37.0	37.0	33.0	37.0
88-89	35.64447792271716	37.0	37.0	37.0	33.0	37.0
90-91	35.582764933363336	37.0	37.0	37.0	33.0	37.0
92-93	35.51568855888617	37.0	35.0	37.0	33.0	37.0
94-95	35.46754074487899	37.0	33.0	37.0	33.0	37.0
96-97	35.44042015782743	37.0	33.0	37.0	33.0	37.0
98-99	35.403644606525276	37.0	33.0	37.0	33.0	37.0
100	35.35306168818022	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	14.0
21	69.0
22	302.0
23	975.0
24	2356.0
25	4997.0
26	9315.0
27	15628.0
28	23364.0
29	33481.0
30	46693.0
31	63846.0
32	86434.0
33	119904.0
34	175781.0
35	284709.0
36	635198.0
37	2058855.0
38	2853233.0
39	138666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7389476009891	20.104363466469817	14.500278158132366	23.65641077440872
2	14.408881163460855	21.116596626153942	41.802544040687074	22.67197816969813
3	17.8413134364796	26.176477768622785	30.877704537825384	25.104504257072236
4	12.287539404613554	18.286838878150366	39.37556994085206	30.050051776384016
5	13.200415461187252	37.74856479402898	35.705855188640804	13.345164556142963
6	29.49507661631542	38.142346165430695	19.03057847431409	13.3319987439398
7	26.098404199318075	33.08908414718365	23.23784610699155	17.574665546506726
8	24.257296425019522	36.40212602531712	21.453026064469825	17.887551485193534
9	25.556995257426728	16.984109739867982	21.417809993734956	36.04108500897033
10-11	23.835039257984572	26.855381879225966	29.229643875195954	20.079934987593507
12-13	24.863842891160065	24.54452559466791	29.183754649574155	21.40787686459787
14-15	22.051439876493518	25.94360912236608	27.038259789014845	24.966691212125557
16-17	21.44113224855222	28.99857274208989	27.657607937714552	21.902687071643342
18-19	21.48654083889319	28.143514948367564	29.42143261050873	20.948511602230514
20-21	22.29379806335534	27.27655997123437	29.416121951500497	21.013520013909798
22-23	21.99563156883171	27.165844657842975	29.252763574056367	21.585760199268947
24-25	21.742889650452206	27.33845679023652	29.33893424281333	21.57971931649794
26-27	21.750030436255024	27.48612267162649	29.178218059231625	21.585628832886858
28-29	21.612742875676137	27.58137566302578	29.229172117751222	21.57670934354686
30-31	21.950663666292957	27.384002316453742	29.13508987542176	21.53024414183154
32-33	21.74018285179399	27.55370711311173	29.04752913384198	21.658580901252293
34-35	21.83304974108296	27.65592393127167	29.01319874405857	21.4978275835868
36-37	21.831160441394974	27.544587841120784	28.998882200363063	21.625369517121182
38-39	21.739310297579838	27.693907232464284	28.974357209781232	21.592425260174647
40-41	21.988948077850893	27.570321087684217	28.840909769163048	21.59982106530184
42-43	21.905117232205132	27.625771816823054	28.638534314273812	21.830576636698
44-45	21.983905554731475	27.773618009502854	28.41824299576381	21.824233440001866
46-47	22.033957379759254	27.772038899669298	28.161777134578823	22.032226585992625
48-49	22.01423472222589	27.911768314158465	28.10673008646197	21.967266877153676
50-51	22.018852887020092	28.012638976231962	27.952887044267605	22.015621092480337
52-53	22.14906601203004	27.895172217014146	27.867822737436487	22.087939033519323
54-55	22.153256790562867	27.790040885510052	27.931122104384613	22.125580219542464
56-57	22.129724651184613	27.88392044714909	27.848599861982308	22.13775503968398
58-59	22.055037156659555	27.930096547552502	27.919266686323308	22.09559960946464
60-61	22.172261658597968	27.910359636246824	27.92715325826196	21.99022544689325
62-63	22.04385619857417	28.003872180863393	27.85839752018358	22.093874100378855
64-65	22.00392693630977	27.98197719117428	27.822063693098354	22.1920321794176
66-67	22.078662818834644	28.09756856821354	27.627416908857246	22.19635170409457
68-69	22.090961931541546	28.05458144857037	27.671987971834188	22.182468648053895
70-71	22.061641339806346	27.811056457678596	27.7207729878367	22.406529214678354
72-73	22.133427355536476	27.798011877463086	27.664679871337672	22.403880895662773
74-75	22.024412842473687	27.917033051929486	27.61991963631796	22.43863446927887
76-77	22.170417979286185	27.836446697539497	27.504134134985403	22.48900118818892
78-79	22.661694667484554	27.89646413871382	27.075341253025847	22.36649994077577
80-81	22.16107719251855	27.92734257134526	27.404720550441102	22.506859685695087
82-83	22.22671947041641	27.902561681750537	27.476757833183147	22.393961014649914
84-85	22.34771161128911	27.95461101229657	27.410279735826652	22.287397640587663
86-87	22.150082687668178	27.928258888318112	27.497119081806886	22.424539342206824
88-89	22.152930537017156	27.93880509626494	27.523431877529536	22.384832489188366
90-91	22.20333858583437	27.854009501763894	27.5419276599398	22.400724252461938
92-93	22.190036857143934	27.83126525892411	27.600918955643877	22.377778928288084
94-95	22.033904427550375	27.96644703507109	27.651146012217232	22.348502525161308
96-97	22.096187828245842	28.04039542286667	27.771192606523808	22.092224142363676
98-99	23.23058507543074	29.930174971461003	27.933482410598504	18.90575754250975
100	22.188803354584433	30.91965374353806	21.69396165803575	25.197581243841753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1522.0
1	1358.0
2	1698.0
3	2357.5
4	3259.5
5	5296.0
6	7032.5
7	8283.0
8	9207.0
9	9397.5
10	8952.5
11	8393.5
12	9264.0
13	12204.0
14	18582.5
15	27079.5
16	34272.5
17	39027.5
18	40433.5
19	38617.5
20	35145.0
21	31367.0
22	29096.0
23	30023.5
24	34257.5
25	41977.0
26	52898.5
27	65668.0
28	78875.0
29	93093.0
30	107692.5
31	121967.5
32	137372.5
33	152774.5
34	168874.5
35	183954.0
36	199271.0
37	213521.5
38	223860.5
39	232687.5
40	242778.5
41	251815.5
42	257489.0
43	263780.5
44	268942.5
45	272358.5
46	274109.5
47	274654.0
48	272604.5
49	265389.5
50	256967.0
51	247154.0
52	234196.0
53	219792.5
54	204334.0
55	187875.5
56	170913.5
57	153246.0
58	135514.5
59	117401.0
60	97896.5
61	79236.5
62	62420.5
63	47613.5
64	35374.5
65	26284.0
66	19743.0
67	15060.5
68	11354.0
69	8023.5
70	5596.0
71	3942.5
72	2724.0
73	1839.0
74	1260.5
75	808.5
76	489.5
77	320.0
78	216.5
79	144.0
80	90.5
81	54.5
82	33.0
83	20.0
84	15.0
85	12.5
86	8.5
87	6.5
88	4.5
89	3.0
90	3.5
91	3.5
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	3.967148349681545E-4
4	0.024581061505142186
5	0.016906155274796737
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008730148070637276
26-27	0.05395417376144713
28-29	0.08716424239128642
30-31	0.07774114949118532
32-33	0.03661669404240568
34-35	0.004535735262256443
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.9130197662117427E-4
56-57	8.366626903315342E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.014029762477954632
74-75	0.028841490602209446
76-77	0.0010041432907144116
78-79	5.108473052844788
80-81	0.0
82-83	0.012892616754988713
84-85	0.051918784029582624
86-87	0.04045078526636781
88-89	0.010259845423981753
90-91	8.57576382422657E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	83.0
20-21	419.0
22-23	853.0
24-25	1874.0
26-27	2823.0
28-29	4445.0
30-31	10358.0
32-33	13840.0
34-35	18538.0
36-37	27059.0
38-39	31183.0
40-41	35888.0
42-43	43302.0
44-45	52239.0
46-47	51239.0
48-49	45329.0
50-51	37651.0
52-53	34184.0
54-55	37145.0
56-57	38601.0
58-59	41286.0
60-61	41482.0
62-63	45023.0
64-65	47847.0
66-67	50420.0
68-69	51307.0
70-71	50065.0
72-73	49188.0
74-75	50889.0
76-77	52262.0
78-79	52301.0
80-81	53407.0
82-83	52007.0
84-85	52395.0
86-87	55237.0
88-89	58905.0
90-91	61335.0
92-93	62805.0
94-95	73882.0
96-97	212829.0
98-99	406641.0
100-101	4445260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.53475270527402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.98631479043307	79.64464564744773
2	6.63628680614598	11.61811448914522
3	1.4276386519662894	3.749039890570797
4	0.466114688824732	1.6320493607427464
5	0.2001268676702319	0.8759027935597423
6	0.09709059575385802	0.5099280773593012
7	0.05712801515051863	0.35004806751206563
8	0.034914118892913744	0.2444959010570992
9	0.021603209142596703	0.17019284129437653
>10	0.07078922153634341	0.9859999471491117
>50	0.001422061978329313	0.08066983433970315
>100	5.207757971666266E-4	0.10235543588839714
>500	2.5098363642954496E-5	0.013818998254648359
>1k	2.5098344285064436E-5	0.022738715679156158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5258262883390556E-5
2	0.0	3.0516525766781112E-5	0.0	0.0	1.5258262883390556E-5
3	0.0	3.0516525766781112E-5	0.0	0.0	1.5258262883390556E-5
4	0.0	7.629131441695278E-5	0.0	0.0	1.5258262883390556E-5
5	1.5258262883390556E-5	9.154957730034334E-5	0.0	0.0	1.5258262883390556E-5
6	4.577478865017167E-5	9.154957730034334E-5	0.0	0.0	1.5258262883390556E-5
7	6.1033051533562224E-5	1.2206610306712445E-4	0.0	3.0516525766781112E-5	1.5258262883390556E-5
8	6.1033051533562224E-5	1.37324365950515E-4	0.0	4.577478865017167E-5	1.5258262883390556E-5
9	6.1033051533562224E-5	2.2887394325085835E-4	0.0	1.068078401837339E-4	3.0516525766781112E-5
10-11	6.1033051533562224E-5	2.2887394325085835E-4	0.0	1.37324365950515E-4	3.0516525766781112E-5
12-13	7.629131441695278E-5	2.2887394325085835E-4	0.0	1.6021176027560083E-4	3.0516525766781112E-5
14-15	7.629131441695278E-5	3.5856917775967806E-4	0.0	1.6784089171729613E-4	5.3403920091866944E-5
16-17	9.154957730034334E-5	3.6619830920137336E-4	0.0	1.8309915460068668E-4	6.1033051533562224E-5
18-19	9.154957730034334E-5	3.6619830920137336E-4	0.0	2.4413220613424892E-4	6.1033051533562224E-5
20-21	9.917870874203861E-5	3.7382744064306866E-4	0.0	3.4331091487628757E-4	6.1033051533562224E-5
22-23	1.1443697162542918E-4	4.0434396640984976E-4	0.0	4.3486049217663085E-4	6.1033051533562224E-5
24-25	1.37324365950515E-4	4.7300614938510725E-4	0.0	5.340392009186695E-4	6.1033051533562224E-5
26-27	1.7547002315899138E-4	5.035226751518883E-4	0.0	6.484761725440986E-4	6.1033051533562224E-5
28-29	1.9835741748407723E-4	5.111518065935836E-4	0.0	9.002375101200429E-4	6.1033051533562224E-5
30-31	1.9835741748407723E-4	5.4929746380206E-4	0.0	0.0014571641053637984	6.1033051533562224E-5
32-33	1.9835741748407723E-4	5.645557266854506E-4	0.0	0.0026625668731516524	6.1033051533562224E-5
34-35	1.9835741748407723E-4	6.408470411024034E-4	0.0	0.0043409757903246134	6.1033051533562224E-5
36-37	2.0598654892577253E-4	7.247674869610514E-4	0.0	0.007240045738168819	6.1033051533562224E-5
38-39	2.5939046901763947E-4	7.858005384946136E-4	0.0	0.02620606650222328	7.629131441695278E-5
40-41	2.7464873190103E-4	9.30754035886824E-4	0.0	0.046034179119189306	7.629131441695278E-5
42-43	2.7464873190103E-4	9.765288245369957E-4	0.0	0.05095496889908276	7.629131441695278E-5
44-45	2.7464873190103E-4	0.0011138531904875107	0.0	0.05872905383817025	7.629131441695278E-5
46-47	2.7464873190103E-4	0.001205402767787854	0.0	0.0680137068027134	7.629131441695278E-5
48-49	2.8990699478442057E-4	0.0012282901621129398	0.0	0.07684061188075485	7.629131441695278E-5
50-51	3.051652576678111E-4	0.001396131053830236	0.0	0.08584298698195528	7.629131441695278E-5
52-53	3.051652576678111E-4	0.0014190184481553219	0.0	0.15830447741517703	7.629131441695278E-5
54-55	3.051652576678111E-4	0.0014342767110387122	0.0	0.25248610506290525	7.629131441695278E-5
56-57	3.051652576678111E-4	0.0014647932368054934	0.0	0.3515885224905269	7.629131441695278E-5
58-59	3.051652576678111E-4	0.0014953097625722746	0.0	0.4576334495300913	7.629131441695278E-5
60-61	3.127943891095064E-4	0.0015563428141058368	0.0	0.4910719326390417	7.629131441695278E-5
62-63	3.204235205512017E-4	0.0016326341285227896	0.0	0.5450404084575942	7.629131441695278E-5
64-65	3.204235205512017E-4	0.001724183705823133	0.0	0.5518379645721446	7.629131441695278E-5
66-67	3.204235205512017E-4	0.0017470711001482187	0.0	0.5568274165350133	7.629131441695278E-5
68-69	3.204235205512017E-4	0.001754700231589914	0.0	0.5611073592738043	7.629131441695278E-5
70-71	3.4331091487628757E-4	0.001754700231589914	0.0	0.5648990376003269	7.629131441695278E-5
72-73	3.814565720847639E-4	0.0017623293630316093	0.0	0.5683702924062983	9.154957730034334E-5
74-75	3.814565720847639E-4	0.0017928458887983903	0.0	0.5708268727305241	9.154957730034334E-5
76-77	3.814565720847639E-4	0.0018615080717736478	0.0	0.5734741813407924	9.154957730034334E-5
78-79	3.814565720847639E-4	0.001914911991865515	0.0	0.5752136233094989	9.154957730034334E-5
80-81	3.814565720847639E-4	0.0019225411233072102	0.0	0.5757476625104176	9.154957730034334E-5
82-83	3.814565720847639E-4	0.0019225411233072102	0.0	0.5761672647397109	9.154957730034334E-5
84-85	3.8908570352645915E-4	0.0019225411233072102	0.0	0.5763961386829617	9.154957730034334E-5
86-87	3.9671483496815446E-4	0.0019988324377241628	0.0	0.5764800591288203	9.154957730034334E-5
88	3.9671483496815446E-4	0.0020903820150245064	0.0	0.576487688260262	9.154957730034334E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9675	0.0	45.402935	1
GTATCAA	17165	0.0	36.327065	1
TCAACGC	20345	0.0	30.08026	4
ATCAACG	20470	0.0	29.938446	3
CAACGCA	20745	0.0	29.473528	5
TATCAAC	21385	0.0	28.938034	2
AACGCAG	21285	0.0	28.74153	6
ACGCAGA	23950	0.0	25.52547	7
CGCAGAG	24275	0.0	25.130787	8
GCAGAGT	28270	0.0	21.518791	9
GAGTACT	17250	0.0	20.054358	12-13
AGAGTAC	25015	0.0	19.771915	10-11
TACATGG	17975	0.0	19.335838	2
GTACATG	18555	0.0	19.331945	1
CAGAGTA	27125	0.0	19.142038	10-11
ACATGGG	18520	0.0	18.234602	3
GTACTTT	19060	0.0	18.16186	14-15
AGTACTT	18735	0.0	17.824516	12-13
CATGGGG	13255	0.0	16.198286	4
TGGTATC	4805	0.0	15.8758745	2
>>END_MODULE