FastQCFastQC Report
Fri 27 May 2016
SRR2138885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7790218
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112100.047.7265171
GTATCAA204000.037.393471
TCAACGC235900.031.8428344
ATCAACG237350.031.703043
CAACGCA241850.031.015595
AACGCAG249250.030.2104786
TATCAAC250150.030.1843072
ACGCAGA278450.026.9649777
CGCAGAG283200.026.6040748
GCAGAGT330700.022.6654349
GAGTACT210350.020.9945212-13
TACATGG201450.019.9828782
AGAGTAC294050.019.86581210-11
GTACATG210000.019.8473031
CAGAGTA318100.019.80774510-11
ACATGGG205350.019.2671873
GTACTTT235550.018.71267114-15
AGTACTT227950.017.99241312-13
TATACCG5250.017.253875
GTGGTAT58700.016.8322051
Produced by FastQC (version 0.11.5)