##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138885_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7790218
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8483478896226	33.0	33.0	33.0	33.0	33.0
2	32.84395609981646	33.0	33.0	33.0	33.0	33.0
3	32.845042333860235	33.0	33.0	33.0	33.0	33.0
4	36.796238949924124	37.0	37.0	37.0	37.0	37.0
5	36.80756135450895	37.0	37.0	37.0	37.0	37.0
6	36.81703990825417	37.0	37.0	37.0	37.0	37.0
7	36.80331371984712	37.0	37.0	37.0	37.0	37.0
8	36.80191979223174	37.0	37.0	37.0	37.0	37.0
9	36.79975913382655	37.0	37.0	37.0	37.0	37.0
10-11	36.781768109698604	37.0	37.0	37.0	37.0	37.0
12-13	36.770806606438995	37.0	37.0	37.0	37.0	37.0
14-15	39.164908414629735	40.0	40.0	40.0	37.0	40.0
16-17	39.13122103900045	40.0	40.0	40.0	37.0	40.0
18-19	39.106956634589686	40.0	40.0	40.0	37.0	40.0
20-21	39.154894315927734	40.0	40.0	40.0	37.0	40.0
22-23	39.18576656942824	40.0	40.0	40.0	37.0	40.0
24-25	39.1905751034229	40.0	40.0	40.0	37.0	40.0
26-27	38.939198581174495	40.0	40.0	40.0	37.0	40.0
28-29	38.9460961710962	40.0	40.0	40.0	37.0	40.0
30-31	38.96349404523653	40.0	40.0	40.0	37.0	40.0
32-33	38.95418213553976	40.0	40.0	40.0	37.0	40.0
34-35	38.93646076924745	40.0	40.0	40.0	37.0	40.0
36-37	38.90683685026229	40.0	40.0	40.0	37.0	40.0
38-39	38.873442263439316	40.0	40.0	40.0	37.0	40.0
40-41	38.827138656221095	40.0	40.0	40.0	37.0	40.0
42-43	38.77331776100324	40.0	37.0	40.0	37.0	40.0
44-45	38.71149414322872	40.0	37.0	40.0	37.0	40.0
46-47	38.64343831034073	40.0	37.0	40.0	37.0	40.0
48-49	38.59565011040632	40.0	37.0	40.0	37.0	40.0
50-51	38.551794055725814	40.0	37.0	40.0	37.0	40.0
52-53	38.490191431159005	40.0	37.0	40.0	37.0	40.0
54-55	38.42288215556354	40.0	37.0	40.0	37.0	40.0
56-57	38.32799827545013	40.0	37.0	40.0	37.0	40.0
58-59	38.20414911312653	40.0	37.0	40.0	37.0	40.0
60-61	38.05630816823769	40.0	37.0	40.0	37.0	40.0
62-63	37.876999647719025	40.0	37.0	40.0	35.0	40.0
64-65	37.664028012434095	38.5	37.0	40.0	33.0	40.0
66-67	37.43845670537711	37.0	37.0	40.0	33.0	40.0
68-69	37.18428504314347	37.0	37.0	40.0	33.0	40.0
70-71	36.929068870269276	37.0	37.0	40.0	33.0	40.0
72-73	36.680963572643485	37.0	37.0	38.5	33.0	40.0
74-75	36.414874795432084	37.0	37.0	37.0	33.0	40.0
76-77	34.72373359303573	37.0	33.0	37.0	30.0	37.0
78-79	31.618182864801952	37.0	33.0	37.0	10.5	37.0
80-81	34.66726626398041	37.0	33.0	37.0	30.0	37.0
82-83	35.6742722966367	37.0	35.0	37.0	33.0	37.0
84-85	35.78337096726709	37.0	37.0	37.0	33.0	37.0
86-87	35.74339758376571	37.0	37.0	37.0	33.0	37.0
88-89	35.66427423112968	37.0	37.0	37.0	33.0	37.0
90-91	35.59718566966677	37.0	37.0	37.0	33.0	37.0
92-93	35.52860672382444	37.0	35.0	37.0	33.0	37.0
94-95	35.479145794975786	37.0	33.0	37.0	33.0	37.0
96-97	35.45482180562867	37.0	33.0	37.0	33.0	37.0
98-99	35.41590022584397	37.0	33.0	37.0	33.0	37.0
100	35.3659141482877	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	25.0
21	85.0
22	348.0
23	1113.0
24	2788.0
25	5767.0
26	10666.0
27	18024.0
28	27821.0
29	39914.0
30	55657.0
31	75454.0
32	102455.0
33	141514.0
34	206300.0
35	335354.0
36	739032.0
37	2422467.0
38	3447203.0
39	158229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.557219579734486	20.309547178268954	14.516885150068969	23.616348091927595
2	14.284426956986312	21.231241539068613	41.98962082960965	22.49471067433543
3	17.70399922261193	26.416059995463527	30.918877577938098	24.96106320398645
4	12.091003905952581	18.484511969065668	39.700776889230596	29.723707235751156
5	13.065199601385242	37.861828785151936	35.850819376210524	13.222152237252299
6	29.160339287039207	38.47294132205286	19.22069446580314	13.14602492510479
7	25.90537260959834	33.1185212018457	23.503462932616262	17.472643255939694
8	24.02084254895049	36.573584975414036	21.641371268429204	17.764201207206266
9	25.4128831824732	17.055170984945477	21.592065844627196	35.93987998795413
10-11	23.62048148074932	27.063902447916092	29.41681477976611	19.89880129156848
12-13	24.706946583523077	24.67260864843577	29.361777809042056	21.258666958999093
14-15	21.850659378209954	26.069719743401276	27.216972618738012	24.862648259650758
16-17	21.26780662620738	29.150352403488576	27.830382923815485	21.75145804648856
18-19	21.296310321482657	28.272539998238816	29.626378876688687	20.804770803589836
20-21	22.07698014697392	27.470392732548945	29.58982847479946	20.862798645677678
22-23	21.721242050136702	27.40046731674546	29.457573779937217	21.420716853180622
24-25	21.573806863997934	27.490252078686556	29.531458358627837	21.404482698687673
26-27	21.579598473793315	27.70048810651578	29.33513863181112	21.384774787879785
28-29	21.442016098017884	27.80155555831331	29.397121327851462	21.359307015817347
30-31	21.787894480274637	27.622769092338253	29.277595409571767	21.311741017815347
32-33	21.553590136149946	27.714766866211715	29.232211899425998	21.49943109821234
34-35	21.655277877907704	27.810236935109423	29.20571222958549	21.328772957397383
36-37	21.693493622917092	27.719174036259947	29.151696043149293	21.435636297673668
38-39	21.54971207405712	27.849794709695647	29.187933573285147	21.41255964296209
40-41	21.798213152782992	27.75013701875627	29.00993332166994	21.441716506790794
42-43	21.72966890344786	27.835877641614637	28.799399864837138	21.635053590100366
44-45	21.826180855552035	27.96948919062373	28.578978138394252	21.625351815429983
46-47	21.857576592514736	27.980090839749067	28.28919758044355	21.873134987292648
48-49	21.82477277378308	28.052701053549633	28.311447776274186	21.811078396393096
50-51	21.887220092437158	28.198622303497935	28.098882192455022	21.815275411609893
52-53	22.00991898494912	28.139391671823276	28.018167615164042	21.83252172806357
54-55	22.009972970898627	28.04322774938664	28.023310708429104	21.923488571285628
56-57	22.02515650970061	28.16721228087407	27.950768240534106	21.85686296889121
58-59	21.938214794895075	28.2319931234703	28.013677113790685	21.816114967843937
60-61	22.059028763348117	28.18792408097429	27.95615732144431	21.796889834233284
62-63	21.951773609771358	28.269693662419463	27.92524343488613	21.853289292923044
64-65	21.93114764862089	28.230723698847292	27.91357505801852	21.924553594513295
66-67	22.031218195501655	28.21982531141971	27.790882433682697	21.958074059395933
68-69	21.97977179825646	28.224225516209273	27.833770121299683	21.962232564234583
70-71	22.014548990942426	27.98106721921646	27.911085205733134	22.093298584107984
72-73	22.0394307939171	28.04500790531702	27.873179731165436	22.04238156960044
74-75	21.941272054190122	28.176840428983894	27.770313401947956	22.111574114878028
76-77	22.01968498848847	28.131499120249764	27.719454757944064	22.1293611333177
78-79	22.50039911519412	28.054226085479744	27.368570465726304	22.076804333599828
80-81	21.994539656554032	28.137677790136827	27.71540261296575	22.15237994034339
82-83	22.052502958710456	28.130669029135763	27.782895904729184	22.033932107424604
84-85	22.164287398737056	28.09666041147128	27.737223316292475	22.00182887349919
86-87	21.992129322764427	28.1287919452045	27.783481372316	22.095597359715068
88-89	22.066715167524087	28.09673542543126	27.781087332658977	22.055462074385677
90-91	22.085260879049105	28.11180427105044	27.787817406730742	22.01511744316971
92-93	22.08599780547102	28.093405336833104	27.794617905730878	22.025978951964994
94-95	21.982751633441364	28.177638739184484	27.78574185033431	22.05386777703984
96-97	22.06240165639282	28.282856536101818	27.87137494892879	21.78336685857657
98-99	23.145434886647642	30.18798411826222	28.06836591807434	18.59821507701579
100	22.087436351975477	31.17339007174278	21.952860678939466	24.786312897342277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1645.0
1	1519.5
2	2038.0
3	2923.5
4	4129.0
5	6660.5
6	8689.0
7	10033.0
8	10997.5
9	11052.0
10	10452.0
11	9813.0
12	10926.5
13	14524.5
14	22436.0
15	33004.0
16	41900.5
17	47728.5
18	49108.0
19	46364.5
20	41735.0
21	37131.0
22	34832.0
23	36353.0
24	41586.0
25	50927.0
26	64711.0
27	81210.0
28	97715.0
29	115543.5
30	133992.5
31	151973.0
32	170870.5
33	189115.5
34	207257.0
35	224277.0
36	242267.5
37	259855.0
38	271211.5
39	280487.5
40	292041.0
41	301893.0
42	308675.5
43	314321.5
44	318201.0
45	321487.0
46	322920.0
47	322237.5
48	317689.5
49	307957.5
50	298054.0
51	284949.5
52	267076.0
53	248845.5
54	229758.0
55	209854.5
56	190337.5
57	170650.5
58	150139.5
59	128787.5
60	106705.0
61	86007.0
62	67859.5
63	51662.5
64	38143.0
65	28086.5
66	20988.0
67	16004.5
68	11897.0
69	8453.0
70	6094.0
71	4363.5
72	3100.0
73	2243.0
74	1535.5
75	993.5
76	665.0
77	418.0
78	261.0
79	172.5
80	109.5
81	75.0
82	57.5
83	43.5
84	28.5
85	18.0
86	12.5
87	10.0
88	8.5
89	6.0
90	3.5
91	2.0
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.7226172618019164E-4
4	0.023734894196799115
5	0.015968744392005463
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008429542252101479
26-27	0.05195253554702938
28-29	0.08428406408646971
30-31	0.07441755436422885
32-33	0.0358140681935402
34-35	0.0041871086601794226
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.8170774682688393E-4
56-57	8.230786874199557E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.013058747431398156
74-75	0.027254087344039748
76-77	9.083592535738766E-4
78-79	4.896124858469924
80-81	0.0
82-83	0.012192032703762947
84-85	0.04842706520585749
86-87	0.038398579260288786
88-89	0.009497550715790162
90-91	2.3641192887310707E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	507.0
22-23	1065.0
24-25	1924.0
26-27	3012.0
28-29	4791.0
30-31	10493.0
32-33	14064.0
34-35	19036.0
36-37	27506.0
38-39	33680.0
40-41	37829.0
42-43	46032.0
44-45	55917.0
46-47	55172.0
48-49	47915.0
50-51	42733.0
52-53	41984.0
54-55	44989.0
56-57	47470.0
58-59	47870.0
60-61	48059.0
62-63	48387.0
64-65	51039.0
66-67	54866.0
68-69	55384.0
70-71	54658.0
72-73	53471.0
74-75	55769.0
76-77	58814.0
78-79	57624.0
80-81	58884.0
82-83	59032.0
84-85	59269.0
86-87	62355.0
88-89	66121.0
90-91	69938.0
92-93	72713.0
94-95	86705.0
96-97	251511.0
98-99	489767.0
100-101	5391762.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.8139014356334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.16997322801917	77.3783719504294
2	6.988799879838424	11.994723680836422
3	1.5864608417635113	4.084211829197579
4	0.5735368531214454	1.9686973993386825
5	0.25871497962035733	1.1100670880531622
6	0.13341905813007304	0.6869525942405478
7	0.08320785856260683	0.4998273681353167
8	0.05460877413683506	0.3748953569039304
9	0.03746537856469653	0.2893545273059604
>10	0.1121204753213765	1.4541046405840439
>50	0.0012512718990189886	0.07070283971197354
>100	3.945889093709241E-4	0.059729862322713635
>500	4.681211303695081E-5	0.028360862940276156
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2836611247592815E-5	0.0
2	0.0	2.567322249518563E-5	0.0	2.567322249518563E-5	0.0
3	0.0	2.567322249518563E-5	0.0	2.567322249518563E-5	0.0
4	0.0	1.0269288998074252E-4	0.0	3.8509833742778444E-5	1.2836611247592815E-5
5	0.0	1.1552950122833532E-4	0.0	3.8509833742778444E-5	1.2836611247592815E-5
6	0.0	1.1552950122833532E-4	0.0	3.8509833742778444E-5	1.2836611247592815E-5
7	0.0	1.1552950122833532E-4	0.0	5.134644499037126E-5	1.2836611247592815E-5
8	0.0	1.1552950122833532E-4	0.0	5.134644499037126E-5	1.2836611247592815E-5
9	0.0	1.4120272372352097E-4	0.0	1.1552950122833532E-4	1.2836611247592815E-5
10-11	0.0	1.4120272372352097E-4	0.0	1.2194780685213173E-4	1.2836611247592815E-5
12-13	0.0	1.5403933497111377E-4	0.0	1.2836611247592813E-4	1.2836611247592815E-5
14-15	0.0	1.797125574662994E-4	0.0	1.2836611247592813E-4	1.2836611247592815E-5
16-17	0.0	1.8613086309009582E-4	0.0	1.2836611247592813E-4	1.2836611247592815E-5
18-19	0.0	2.0538577996148503E-4	0.0	1.5403933497111377E-4	2.567322249518563E-5
20-21	0.0	2.1822239120907784E-4	0.0	1.9896747433768863E-4	2.567322249518563E-5
22-23	0.0	2.8882375307083834E-4	0.0	2.4389561370426348E-4	2.567322249518563E-5
24-25	0.0	3.2733358681361676E-4	0.0	2.6956883619944907E-4	2.567322249518563E-5
26-27	0.0	3.4658850368500597E-4	0.0	2.9524205869463474E-4	2.567322249518563E-5
28-29	0.0	3.5942511493259883E-4	0.0	4.043532542991736E-4	2.567322249518563E-5
30-31	6.418305623796407E-6	3.8509833742778445E-4	0.0	6.418305623796408E-4	2.567322249518563E-5
32-33	1.2836611247592815E-5	4.0435325429917366E-4	0.0	0.0012900794303830777	2.567322249518563E-5
34-35	1.2836611247592815E-5	4.8779122740852697E-4	0.0	0.002471047665161617	2.567322249518563E-5
36-37	1.2836611247592815E-5	5.776475061416767E-4	0.0	0.004024277626120348	3.209152811898203E-5
38-39	1.2836611247592815E-5	6.289939511320479E-4	0.0	0.006476070374410574	3.8509833742778444E-5
40-41	1.2836611247592815E-5	6.546671736272335E-4	0.0	0.009088320763295712	3.8509833742778444E-5
42-43	1.2836611247592815E-5	7.060136186176048E-4	0.0	0.011963721682756504	3.8509833742778444E-5
44-45	1.9254916871389222E-5	8.279614254697366E-4	0.0	0.01493539718657424	4.4928139366574854E-5
46-47	2.567322249518563E-5	8.857261760839042E-4	0.0	0.0187542890327331	5.776475061416766E-5
48-49	2.567322249518563E-5	8.921444817077005E-4	0.0	0.02229077543144492	6.418305623796407E-5
50-51	3.209152811898203E-5	9.948373716884432E-4	0.0	0.025801588607661557	6.418305623796407E-5
52-53	3.8509833742778444E-5	0.0010140922885598324	0.0	0.02954987909195866	7.060136186176047E-5
54-55	3.8509833742778444E-5	0.0010333472054312215	0.0	0.033330261104374745	7.701966748555689E-5
56-57	3.8509833742778444E-5	0.0010654387335502036	0.0	0.03726468245176194	7.701966748555689E-5
58-59	3.8509833742778444E-5	0.0010911119560453892	0.0	0.04142374449598202	7.701966748555689E-5
60-61	4.4928139366574854E-5	0.0011167851785405748	0.0	0.046044924545115426	7.701966748555689E-5
62-63	6.418305623796407E-5	0.001136040095411964	0.0	0.050711032733615415	8.98562787331497E-5
64-65	6.418305623796407E-5	0.0012066414572737246	0.0	0.05579433078766217	8.98562787331497E-5
66-67	7.701966748555689E-5	0.001213059762897521	0.0	0.06054387694927151	8.98562787331497E-5
68-69	7.701966748555689E-5	0.0012772428191354852	0.0	0.06472861221598677	8.98562787331497E-5
70-71	7.701966748555689E-5	0.001309334347254467	0.0	0.06846406608903627	8.98562787331497E-5
72-73	7.701966748555689E-5	0.00132217095850206	0.0	0.07135872192536846	8.98562787331497E-5
74-75	7.701966748555689E-5	0.0013285892641258564	0.0	0.07379767806241108	8.98562787331497E-5
76-77	8.343797310935329E-5	0.0013606807922448383	0.0	0.0762815623388203	8.98562787331497E-5
78-79	8.98562787331497E-5	0.0013863540147400239	0.0	0.07786046552227421	8.98562787331497E-5
80-81	8.98562787331497E-5	0.0013991906259876168	0.0	0.0784445313340397	9.627458435694611E-5
82-83	8.98562787331497E-5	0.001418445542859006	0.0	0.07870768186461534	1.1552950122833532E-4
84-85	8.98562787331497E-5	0.001450537070977988	0.0	0.07891306764457683	1.1552950122833532E-4
86-87	8.98562787331497E-5	0.00148262859909697	0.0	0.078970832395191	1.1552950122833532E-4
88	8.98562787331497E-5	0.0015275567384635449	0.0	0.078970832395191	1.1552950122833532E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11210	0.0	47.726517	1
GTATCAA	20400	0.0	37.39347	1
TCAACGC	23590	0.0	31.842834	4
ATCAACG	23735	0.0	31.70304	3
CAACGCA	24185	0.0	31.01559	5
AACGCAG	24925	0.0	30.210478	6
TATCAAC	25015	0.0	30.184307	2
ACGCAGA	27845	0.0	26.964977	7
CGCAGAG	28320	0.0	26.604074	8
GCAGAGT	33070	0.0	22.665434	9
GAGTACT	21035	0.0	20.99452	12-13
TACATGG	20145	0.0	19.982878	2
AGAGTAC	29405	0.0	19.865812	10-11
GTACATG	21000	0.0	19.847303	1
CAGAGTA	31810	0.0	19.807745	10-11
ACATGGG	20535	0.0	19.267187	3
GTACTTT	23555	0.0	18.712671	14-15
AGTACTT	22795	0.0	17.992413	12-13
TATACCG	525	0.0	17.25387	5
GTGGTAT	5870	0.0	16.832205	1
>>END_MODULE