FastQCFastQC Report
Fri 27 May 2016
SRR2138884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233661
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.053.9946251
GTATCAA194050.039.292881
ATCAACG231700.032.2610743
TCAACGC241800.030.8589174
TATCAAC247350.030.3931852
CAACGCA247850.030.169325
AACGCAG251500.029.7618066
ACGCAGA256400.029.1596537
CGCAGAG297550.025.0982258
GTGGTAT46200.024.8345581
TGGTATC43450.024.2386762
GCAGAGT341700.021.7551639
GTACATG220750.020.5186391
GAGTACT215850.020.39199412-13
TACATGG219850.019.9794942
ACATGGG217100.019.6802833
AGAGTAC309200.019.43970910-11
CAGAGTA332050.019.33282310-11
GTACTTT236800.018.45317314-15
AGTACTT229850.018.06070512-13
Produced by FastQC (version 0.11.5)