##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7233661
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90292674760401	33.0	33.0	33.0	33.0	33.0
2	32.9610353595503	33.0	33.0	33.0	33.0	33.0
3	33.62678123843514	33.0	33.0	33.0	33.0	37.0
4	36.79039990953405	37.0	37.0	37.0	37.0	37.0
5	36.80238706237409	37.0	37.0	37.0	37.0	37.0
6	36.80688271125783	37.0	37.0	37.0	37.0	37.0
7	36.79831540350038	37.0	37.0	37.0	37.0	37.0
8	36.78936516378083	37.0	37.0	37.0	37.0	37.0
9	36.7931990454073	37.0	37.0	37.0	37.0	37.0
10-11	36.7885052257771	37.0	37.0	37.0	37.0	37.0
12-13	37.045106827649235	37.0	37.0	37.0	37.0	38.5
14-15	39.13445411666375	40.0	40.0	40.0	37.0	40.0
16-17	39.096748935290165	40.0	40.0	40.0	37.0	40.0
18-19	39.0786809058373	40.0	40.0	40.0	37.0	40.0
20-21	39.133868382736466	40.0	40.0	40.0	37.0	40.0
22-23	39.16367886599534	40.0	40.0	40.0	37.0	40.0
24-25	39.149111303738856	40.0	40.0	40.0	37.0	40.0
26-27	38.891446586649394	40.0	40.0	40.0	37.0	40.0
28-29	38.92178320144184	40.0	40.0	40.0	37.0	40.0
30-31	38.92779500725065	40.0	40.0	40.0	37.0	40.0
32-33	38.911855879536375	40.0	40.0	40.0	37.0	40.0
34-35	38.889025544506595	40.0	40.0	40.0	37.0	40.0
36-37	38.8543908411573	40.0	40.0	40.0	37.0	40.0
38-39	38.81476614813643	40.0	40.0	40.0	37.0	40.0
40-41	38.764113183983795	40.0	37.0	40.0	37.0	40.0
42-43	38.705265302993965	40.0	37.0	40.0	37.0	40.0
44-45	38.63660140849288	40.0	37.0	40.0	37.0	40.0
46-47	38.562094396495	40.0	37.0	40.0	37.0	40.0
48-49	38.5067578452563	40.0	37.0	40.0	37.0	40.0
50-51	38.457300360265215	40.0	37.0	40.0	37.0	40.0
52-53	38.3907236180372	40.0	37.0	40.0	37.0	40.0
54-55	38.313747548996595	40.0	37.0	40.0	37.0	40.0
56-57	38.21352320826039	40.0	37.0	40.0	37.0	40.0
58-59	38.0792173050441	40.0	37.0	40.0	37.0	40.0
60-61	37.92255370643176	40.0	37.0	40.0	37.0	40.0
62-63	37.736077703466286	38.5	37.0	40.0	33.0	40.0
64-65	37.51643909980457	37.0	37.0	40.0	33.0	40.0
66-67	37.28655141417613	37.0	37.0	40.0	33.0	40.0
68-69	37.030354729768646	37.0	37.0	40.0	33.0	40.0
70-71	36.77945667445239	37.0	37.0	40.0	33.0	40.0
72-73	36.52728503228575	37.0	37.0	37.0	33.0	40.0
74-75	36.042536517325026	37.0	37.0	37.0	33.0	40.0
76-77	34.21062662342739	37.0	33.0	37.0	30.0	37.0
78-79	31.877888198432824	37.0	33.0	37.0	10.5	37.0
80-81	34.72261409655802	37.0	33.0	37.0	30.0	37.0
82-83	35.62204197499756	37.0	35.0	37.0	33.0	37.0
84-85	35.71525214733767	37.0	37.0	37.0	33.0	37.0
86-87	35.673836877152496	37.0	37.0	37.0	33.0	37.0
88-89	35.597185824570516	37.0	37.0	37.0	33.0	37.0
90-91	35.525932101885054	37.0	35.0	37.0	33.0	37.0
92-93	35.458878877275815	37.0	33.0	37.0	33.0	37.0
94-95	35.41358332442648	37.0	33.0	37.0	33.0	37.0
96-97	35.38773854247829	37.0	33.0	37.0	33.0	37.0
98-99	35.34135389382729	37.0	33.0	37.0	33.0	37.0
100	35.2779938642543	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	9.0
20	21.0
21	99.0
22	407.0
23	1150.0
24	2750.0
25	5919.0
26	10847.0
27	18013.0
28	27328.0
29	39557.0
30	54390.0
31	74250.0
32	100093.0
33	138338.0
34	200059.0
35	326537.0
36	717676.0
37	2267484.0
38	3075568.0
39	173162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.02485275747604	0.0	24.51359231944508	29.461554923078882
2	14.949708347399623	23.892951869371927	38.50571900100724	22.651620782221205
3	16.508178114367794	24.66251366936114	33.20805216960929	25.621256046661774
4	12.953580803561056	23.105408622335595	37.54149608241584	26.39951449168751
5	17.278788163690812	36.412232843794676	32.254550884460215	14.054428108054298
6	27.886999404589186	37.32186233222707	20.40564245407685	14.385495809106896
7	26.010895451141547	33.11493308851493	22.4639501353464	18.41022132499712
8	24.21599519247584	32.004292155797735	22.25394582356016	21.52576682816626
9	23.930081876936175	20.291619969473274	23.767868026992144	32.01043012659841
10-11	24.46546085032185	26.049769266212504	28.82753283572454	20.65723704774111
12-13	24.936425690946812	24.304843702241506	28.631449552308297	22.127281054503385
14-15	22.390999522924837	26.627720043833957	26.893443029746628	24.08783740349458
16-17	21.358949500121724	28.87990603927942	27.772942359339204	21.988202101259652
18-19	21.467597315881587	27.827883809855592	29.682166427506964	21.02235244675586
20-21	22.259139243929226	27.16563008766378	29.617102506335293	20.958128162071706
22-23	22.095313333212587	26.929479271381705	29.403485976554705	21.571721418850995
24-25	21.75023837837565	27.14114053602409	29.59967576806795	21.508945317532305
26-27	21.690610376008763	27.358859172456572	29.394603873096887	21.555926578437777
28-29	21.67227019668858	27.359537468968192	29.409297092593818	21.55889524174941
30-31	21.894010458201883	27.17998486562786	29.4288053390771	21.49719933709315
32-33	21.685893463749622	27.494090789460536	29.305186779359488	21.51482896743035
34-35	21.882000330208683	27.37217870827203	29.161244906211138	21.584576055308148
36-37	21.83848602557712	27.377034791068827	29.206086106625477	21.57839307672857
38-39	21.65900500175293	27.68397403379878	29.140562802842833	21.516458161605456
40-41	22.013266865435607	27.37790143578011	28.89378866664925	21.715043032135032
42-43	21.86320352844411	27.450681002233818	28.88196202069968	21.804153448622394
44-45	22.02510590964968	27.735778471142186	28.48412701526119	21.754988603946945
46-47	22.121469946447757	27.616493745046455	28.201159651270334	22.06087665723545
48-49	21.95635917961184	27.78869751086142	28.233048817827346	22.021894491699392
50-51	22.005095895433723	28.09634179165565	27.939196941061482	21.95936537184915
52-53	22.275230955755788	27.772546663493486	27.821811252372818	22.130411128377908
54-55	22.170987776516927	27.70962076781028	27.961563894693743	22.15782756097905
56-57	22.155081389022254	28.049482835395985	27.801980878575037	21.99345489700672
58-59	22.23243628065516	27.8231900541971	27.85276354867551	22.091610116472225
60-61	22.135753074850157	27.90562760675288	27.91175249072329	22.046866827673675
62-63	22.114799393906573	28.066897231707994	27.754994122194105	22.06330925219133
64-65	22.230116836465896	27.810423384642974	27.74862048883421	22.210839290056924
66-67	22.183170713074375	27.903265183944452	27.708284301599107	22.205279801382062
68-69	22.22603920985869	27.977614351984048	27.70195962245398	22.09438681570328
70-71	22.209393293925917	27.616162192486488	27.761655406173258	22.412789107414337
72-73	22.202456455644782	27.7224241475865	27.72612281962098	22.34899657714774
74-75	22.091059880143074	27.932972742986646	27.68208140696257	22.293885969907713
76-77	22.444235618356277	27.69662462793049	27.464843356363524	22.39429639734971
78-79	22.57206831359327	27.786341031758727	27.364605465905118	22.276985188742884
80-81	22.234869897636518	27.918832924471577	27.50151967223382	22.344777505658094
82-83	22.34527749750148	27.67763541697744	27.64183424636511	22.33525283915597
84-85	22.40554579299776	27.67495706152815	27.671744290823007	22.247752854651083
86-87	22.245436814679703	27.760098403885607	27.649351907628407	22.345112873806283
88-89	22.348144546170214	27.653842909621662	27.589580420419672	22.40843212378845
90-91	22.348180960813576	27.603338015140473	27.731593417118024	22.316887606927928
92-93	22.319215127571216	27.67199923407702	27.682845163425508	22.32594047492626
94-95	22.362596241761423	27.556518316382544	27.680366151141488	22.400519290714545
96-97	22.389907120318732	27.835710114987883	27.844582247008088	21.929800517685297
98-99	23.44372017070748	29.929639812958747	27.36841979396047	19.258220222373307
100	22.572083092513218	30.277244711869244	21.792624779901615	25.358047415715916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1390.0
1	1269.5
2	1889.0
3	2832.0
4	3922.5
5	6357.5
6	8419.0
7	10040.0
8	11498.5
9	11849.5
10	11228.0
11	10538.0
12	11511.5
13	14612.5
14	22113.5
15	33368.0
16	43422.5
17	49700.0
18	50938.0
19	47922.5
20	43195.0
21	38051.0
22	34678.5
23	35117.5
24	39665.5
25	48326.5
26	60417.5
27	74404.5
28	89162.5
29	104838.5
30	120528.0
31	135972.0
32	152117.5
33	168407.5
34	184164.0
35	198790.0
36	215375.5
37	230216.5
38	239469.0
39	248447.0
40	259548.5
41	269413.5
42	275312.5
43	280251.0
44	284801.5
45	288366.0
46	289532.5
47	289671.5
48	288585.0
49	283182.0
50	276436.0
51	267706.5
52	255275.0
53	241365.0
54	226550.0
55	211016.0
56	194970.5
57	177194.5
58	158667.5
59	138954.0
60	117596.0
61	96633.0
62	76910.0
63	59582.5
64	44845.0
65	33100.0
66	24724.5
67	19030.5
68	14495.0
69	10515.5
70	7543.0
71	5358.0
72	3724.5
73	2532.0
74	1703.5
75	1172.0
76	767.0
77	465.5
78	293.5
79	217.0
80	147.5
81	92.5
82	72.0
83	56.0
84	44.0
85	34.0
86	23.0
87	15.0
88	9.0
89	4.0
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3501239552143789E-4
2	7.60334220804652E-4
3	0.004575829583387997
4	0.022851499399819813
5	0.013838082818644667
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.3824258560084583E-5
20-21	2.07368751823981E-5
22-23	5.875874839994744E-4
24-25	0.014291324536828336
26-27	0.05831142533913962
28-29	0.08650423255570634
30-31	0.07232701897631848
32-33	0.03274881327181737
34-35	0.0036649873328440654
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.4686117646229489E-5
54-55	5.172433030477527E-4
56-57	7.587691395796002E-4
58-59	7.490752852871951E-6
60-61	0.0
62-63	7.595535890881923E-6
64-65	7.649819418362811E-6
66-67	6.16710590593668E-5
68-69	1.9430329173869817E-4
70-71	0.0016379866181979374
72-73	0.017581406852323688
74-75	0.07028625333632614
76-77	0.9438836033878928
78-79	3.9674718927951456
80-81	0.0011543235639355262
82-83	0.01731908067741733
84-85	0.0493104790272824
86-87	0.03602070352468101
88-89	0.008408741789242375
90-91	2.5826695298715E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	102.0
20-21	401.0
22-23	937.0
24-25	2222.0
26-27	3149.0
28-29	5750.0
30-31	11251.0
32-33	15377.0
34-35	21586.0
36-37	29259.0
38-39	34632.0
40-41	37889.0
42-43	47049.0
44-45	57467.0
46-47	56200.0
48-49	48489.0
50-51	42425.0
52-53	41901.0
54-55	44421.0
56-57	46744.0
58-59	46261.0
60-61	46012.0
62-63	46005.0
64-65	49275.0
66-67	52444.0
68-69	53363.0
70-71	52482.0
72-73	52136.0
74-75	53828.0
76-77	56191.0
78-79	56709.0
80-81	56552.0
82-83	56315.0
84-85	57145.0
86-87	60702.0
88-89	65863.0
90-91	68402.0
92-93	71703.0
94-95	92460.0
96-97	342351.0
98-99	1307088.0
100-101	3943123.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.54570417416303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8828999570384	76.89095970040698
2	7.213904642652675	12.342371050019741
3	1.6595388090164833	4.258992480650008
4	0.5737741139324962	1.9633564245304738
5	0.2655404487477417	1.1357922337424406
6	0.14151415448602728	0.7263556797671097
7	0.08038684583426642	0.4813724533262543
8	0.04578377656333743	0.31332843246905945
9	0.031166582054188523	0.23995504876746504
>10	0.10311320555478429	1.359740286340716
>50	0.001646589722012961	0.09086518375551055
>100	6.043667473206403E-4	0.10628628601539584
>500	7.590494185200917E-5	0.03372208153590472
>1k	5.0602708440057344E-5	0.056902658672979334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3824258560084583E-5
2	0.0	4.1472775680253744E-5	0.0	0.0	1.3824258560084583E-5
3	0.0	4.1472775680253744E-5	0.0	0.0	1.3824258560084583E-5
4	0.0	4.1472775680253744E-5	0.0	1.3824258560084583E-5	2.7648517120169165E-5
5	0.0	4.1472775680253744E-5	0.0	1.3824258560084583E-5	2.7648517120169165E-5
6	6.912129280042291E-5	4.1472775680253744E-5	0.0	1.3824258560084583E-5	2.7648517120169165E-5
7	6.912129280042291E-5	4.1472775680253744E-5	0.0	1.3824258560084583E-5	2.7648517120169165E-5
8	6.912129280042291E-5	4.1472775680253744E-5	0.0	1.3824258560084583E-5	2.7648517120169165E-5
9	8.294555136050749E-5	6.912129280042291E-5	0.0	6.912129280042291E-5	5.529703424033833E-5
10-11	8.294555136050749E-5	8.294555136050749E-5	0.0	8.294555136050749E-5	5.529703424033833E-5
12-13	1.1059406848067666E-4	8.294555136050749E-5	0.0	8.294555136050749E-5	5.529703424033833E-5
14-15	1.1059406848067666E-4	1.1750619776071896E-4	0.0	8.294555136050749E-5	6.220916352038061E-5
16-17	1.3133045632080355E-4	1.3824258560084582E-4	0.0	8.294555136050749E-5	6.912129280042291E-5
18-19	1.3824258560084582E-4	1.3824258560084582E-4	0.0	8.294555136050749E-5	6.912129280042291E-5
20-21	1.3824258560084582E-4	1.3824258560084582E-4	0.0	9.676980992059207E-5	6.912129280042291E-5
22-23	1.520668441609304E-4	1.6589110272101498E-4	0.0	1.2441832704076125E-4	6.912129280042291E-5
24-25	1.9353961984118416E-4	1.7971536128109957E-4	0.0	1.4515471488088812E-4	8.294555136050749E-5
26-27	1.9353961984118416E-4	1.9353961984118416E-4	0.0	1.7971536128109957E-4	8.294555136050749E-5
28-29	2.0736387840126873E-4	1.9353961984118416E-4	0.0	3.3178220544203E-4	8.294555136050749E-5
30-31	2.2118813696135332E-4	2.0045174912122646E-4	0.0	7.11949315844356E-4	8.294555136050749E-5
32-33	2.488366540815225E-4	2.0736387840126873E-4	0.0	0.0013686015974483736	8.294555136050749E-5
34-35	2.7648517120169164E-4	2.557487833615648E-4	0.0	0.002536751445775521	8.294555136050749E-5
36-37	3.041336883218608E-4	3.041336883218608E-4	0.0	0.004202574602265714	8.294555136050749E-5
38-39	3.041336883218608E-4	3.1795794688194536E-4	0.0	0.006753150306601318	1.1059406848067666E-4
40-41	3.179579468819454E-4	3.870792396823683E-4	0.0	0.009400495820857516	1.2441832704076125E-4
42-43	3.179579468819454E-4	4.769369203229181E-4	0.0	0.012338150764875488	1.3133045632080355E-4
44-45	3.179579468819454E-4	5.667946009634679E-4	0.0	0.01598775502473782	1.3824258560084582E-4
46-47	3.179579468819454E-4	5.667946009634679E-4	0.0	0.019519853086839428	1.3824258560084582E-4
48-49	3.248700761619877E-4	5.667946009634679E-4	0.0	0.02320401799310197	1.520668441609304E-4
50-51	3.386943347220723E-4	6.082673766437216E-4	0.0	0.02616240932496007	1.520668441609304E-4
52-53	3.5943072256219914E-4	6.082673766437216E-4	0.0	0.02995716829970329	2.0045174912122646E-4
54-55	3.5943072256219914E-4	6.151795059237639E-4	0.0	0.03355838765460532	2.488366540815225E-4
56-57	3.6634285184224146E-4	6.359158937638908E-4	0.0	0.03749138921494939	2.488366540815225E-4
58-59	3.7325498112228373E-4	6.428280230439331E-4	0.0	0.04194280047129662	2.488366540815225E-4
60-61	3.939913689624106E-4	6.497401523239754E-4	0.0	0.04601404461724153	2.488366540815225E-4
62-63	4.2855201536262205E-4	6.497401523239754E-4	0.0	0.050023079599666065	2.6266091264160704E-4
64-65	4.2855201536262205E-4	7.672463500846944E-4	0.0	0.05419109355553156	2.6957304192164937E-4
66-67	4.2855201536262205E-4	7.741584793647366E-4	0.0	0.05851117435555799	2.7648517120169164E-4
68-69	4.4237627392270664E-4	7.810706086447789E-4	0.0	0.0624027031402218	2.7648517120169164E-4
70-71	4.4237627392270664E-4	7.879827379248212E-4	0.0	0.06614216508072468	2.7648517120169164E-4
72-73	4.5620053248279123E-4	7.948948672048635E-4	0.0	0.06892084105130168	3.179579468819454E-4
74-75	4.5620053248279123E-4	8.156312550449904E-4	0.0	0.07107742538667489	3.179579468819454E-4
76-77	4.631126617628335E-4	8.571040307252441E-4	0.0	0.07346210998828948	3.248700761619877E-4
78-79	4.7002479104287577E-4	8.709282892853286E-4	0.0	0.07498969055917881	3.3178220544202995E-4
80-81	4.7002479104287577E-4	8.709282892853286E-4	0.0	0.07554266090158221	3.5943072256219914E-4
82-83	4.97673308163045E-4	8.709282892853286E-4	0.0	0.0759159158827045	3.870792396823683E-4
84-85	4.97673308163045E-4	9.193131942456247E-4	0.0	0.07607489485614546	3.870792396823683E-4
86-87	4.97673308163045E-4	9.26225323525667E-4	0.0	0.07610254337326562	3.870792396823683E-4
88	4.97673308163045E-4	9.815223577660053E-4	0.0	0.07610254337326562	3.870792396823683E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10260	0.0	53.994625	1
GTATCAA	19405	0.0	39.29288	1
ATCAACG	23170	0.0	32.261074	3
TCAACGC	24180	0.0	30.858917	4
TATCAAC	24735	0.0	30.393185	2
CAACGCA	24785	0.0	30.16932	5
AACGCAG	25150	0.0	29.761806	6
ACGCAGA	25640	0.0	29.159653	7
CGCAGAG	29755	0.0	25.098225	8
GTGGTAT	4620	0.0	24.834558	1
TGGTATC	4345	0.0	24.238676	2
GCAGAGT	34170	0.0	21.755163	9
GTACATG	22075	0.0	20.518639	1
GAGTACT	21585	0.0	20.391994	12-13
TACATGG	21985	0.0	19.979494	2
ACATGGG	21710	0.0	19.680283	3
AGAGTAC	30920	0.0	19.439709	10-11
CAGAGTA	33205	0.0	19.332823	10-11
GTACTTT	23680	0.0	18.453173	14-15
AGTACTT	22985	0.0	18.060705	12-13
>>END_MODULE