FastQCFastQC Report
Fri 27 May 2016
SRR2138883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7653920
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116600.050.9094161
GTATCAA211600.038.4303131
TCAACGC252950.031.5511574
ATCAACG254900.031.275943
TATCAAC262000.030.7247662
CAACGCA259850.030.6727315
AACGCAG267650.029.9019036
ACGCAGA298700.026.7502777
CGCAGAG303300.026.3587888
CTTATAC54450.026.2245671
GTGGTAT52450.024.0990731
TGGTATC50450.023.6007692
GCAGAGT353750.022.5508759
TATACAC83300.022.1654723
GAGTACT222950.021.10111212-13
CAGAGTA342650.020.07234210-11
GTACATG222300.019.8912751
TACATGG221550.019.5302332
AGAGTAC321650.019.4456410-11
GTACTTT245450.019.10550914-15
Produced by FastQC (version 0.11.5)