FastQCFastQC Report
Fri 27 May 2016
SRR2138882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490898
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.048.9877471
GTATCAA138450.036.664231
TCAACGC161850.030.6729054
ATCAACG162550.030.5937023
CAACGCA167050.029.7608935
TATCAAC171150.029.4331232
AACGCAG172250.028.8826586
ACGCAGA196600.025.3490877
CGCAGAG199000.025.043378
CTCGTAT42050.021.4556249
GTGGTAT37300.021.3181021
GCAGAGT234600.021.2247859
CTTATAC35850.021.221191
AATCTCG43500.021.0367286
TCTCGTA43200.020.7850158
GAGTACT143450.020.33560412-13
TGGTATC37400.019.5372282
GTACATG151950.019.3765071
TACATGG149000.019.269742
ATCTCGT46600.019.2685137
Produced by FastQC (version 0.11.5)