##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5490898
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.851258573734206	33.0	33.0	33.0	33.0	33.0
2	32.84828474322415	33.0	33.0	33.0	33.0	33.0
3	32.84890103585971	33.0	33.0	33.0	33.0	33.0
4	36.79792285342033	37.0	37.0	37.0	37.0	37.0
5	36.80673999043508	37.0	37.0	37.0	37.0	37.0
6	36.817915211683044	37.0	37.0	37.0	37.0	37.0
7	36.805604839135604	37.0	37.0	37.0	37.0	37.0
8	36.7923796799722	37.0	37.0	37.0	37.0	37.0
9	36.79167633418068	37.0	37.0	37.0	37.0	37.0
10-11	36.783210232643185	37.0	37.0	37.0	37.0	37.0
12-13	36.770860158028796	37.0	37.0	37.0	37.0	37.0
14-15	39.1632806692093	40.0	40.0	40.0	37.0	40.0
16-17	39.12939049313974	40.0	40.0	40.0	37.0	40.0
18-19	39.09191502009325	40.0	40.0	40.0	37.0	40.0
20-21	39.140990707945264	40.0	40.0	40.0	37.0	40.0
22-23	39.1800312161787	40.0	40.0	40.0	37.0	40.0
24-25	39.18571432302879	40.0	40.0	40.0	37.0	40.0
26-27	38.90981409542076	40.0	40.0	40.0	37.0	40.0
28-29	38.927060918459674	40.0	40.0	40.0	37.0	40.0
30-31	38.94324547510489	40.0	40.0	40.0	37.0	40.0
32-33	38.93826002850663	40.0	40.0	40.0	37.0	40.0
34-35	38.919635156498174	40.0	40.0	40.0	37.0	40.0
36-37	38.891105289601995	40.0	40.0	40.0	37.0	40.0
38-39	38.85303918696325	40.0	40.0	40.0	37.0	40.0
40-41	38.81451654420831	40.0	40.0	40.0	37.0	40.0
42-43	38.76272790317638	40.0	37.0	40.0	37.0	40.0
44-45	38.69875225500353	40.0	37.0	40.0	37.0	40.0
46-47	38.62832661376724	40.0	37.0	40.0	37.0	40.0
48-49	38.57913264342447	40.0	37.0	40.0	37.0	40.0
50-51	38.527021125322776	40.0	37.0	40.0	37.0	40.0
52-53	38.45753427522278	40.0	37.0	40.0	37.0	40.0
54-55	38.38802991269577	40.0	37.0	40.0	37.0	40.0
56-57	38.28724027707604	40.0	37.0	40.0	37.0	40.0
58-59	38.15614465216841	40.0	37.0	40.0	37.0	40.0
60-61	38.00397150450599	40.0	37.0	40.0	37.0	40.0
62-63	37.82346706798161	40.0	37.0	40.0	35.0	40.0
64-65	37.6136983160283	37.0	37.0	40.0	33.0	40.0
66-67	37.39110587211331	37.0	37.0	40.0	33.0	40.0
68-69	37.13820540637699	37.0	37.0	40.0	33.0	40.0
70-71	36.890158458892984	37.0	37.0	40.0	33.0	40.0
72-73	36.64935752202757	37.0	37.0	38.5	33.0	40.0
74-75	36.385828537422725	37.0	37.0	37.0	33.0	40.0
76-77	34.711208953865906	37.0	33.0	37.0	30.0	37.0
78-79	30.83170633337032	37.0	33.0	37.0	8.5	37.0
80-81	34.400433011429016	37.0	33.0	37.0	30.0	37.0
82-83	35.60480702105842	37.0	35.0	37.0	33.0	37.0
84-85	35.75902634078018	37.0	37.0	37.0	33.0	37.0
86-87	35.727492129168674	37.0	37.0	37.0	33.0	37.0
88-89	35.648462667158256	37.0	37.0	37.0	33.0	37.0
90-91	35.5826643818594	37.0	37.0	37.0	33.0	37.0
92-93	35.51656948661111	37.0	35.0	37.0	33.0	37.0
94-95	35.47561493448397	37.0	33.0	37.0	33.0	37.0
96-97	35.45343408371696	37.0	33.0	37.0	33.0	37.0
98-99	35.41338308063767	37.0	33.0	37.0	33.0	37.0
100	35.36308816395186	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	0.0
19	4.0
20	13.0
21	54.0
22	258.0
23	840.0
24	1999.0
25	4154.0
26	7778.0
27	12868.0
28	19883.0
29	28802.0
30	39593.0
31	53758.0
32	73388.0
33	102338.0
34	149234.0
35	245512.0
36	551746.0
37	1766450.0
38	2326662.0
39	105561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80835265925537	20.069194510624673	14.441535792506071	23.680917037613884
2	14.37538996353602	20.9768420393167	41.805912256975084	22.84185574017219
3	17.892260778220205	26.32002328220863	30.636811386427844	25.150904553143317
4	12.453235765337677	18.305530280052572	39.02989431651852	30.21133963809123
5	13.241748964061037	37.77389836368184	35.51793851561773	13.4664141566394
6	29.441905495239578	38.12981046087543	18.979919131624737	13.448364912260253
7	26.110410355464627	33.13095599299058	22.95589901688212	17.802734634662674
8	24.207370087734283	36.39579536899065	21.527079905691203	17.86975463758387
9	25.343723376394898	17.036903617586777	21.296407254332532	36.322965751685786
10-11	23.808537328502553	26.886103147426887	29.046578537791085	20.258780986279476
12-13	24.976151441895297	24.438188434751474	29.05384510875999	21.531815014593242
14-15	22.04429038747396	25.932588804235664	26.871151130470828	25.151969677819547
16-17	21.609762191903766	28.990039516304982	27.382415408190063	22.01778288360119
18-19	21.642980802047315	28.05633067669441	29.187320543925605	21.11336797733267
20-21	22.321640697145305	27.163344405564	29.297901830956658	21.217113066334033
22-23	21.932485600468237	27.186612850009457	29.251337273383875	21.629564276138428
24-25	21.796237312779034	27.313909448946927	29.2233358057931	21.666517432480944
26-27	21.914005173799936	27.44644351388157	29.004490158294942	21.63506115402355
28-29	21.618131644596005	27.543158289569657	29.280344894144385	21.558365171689957
30-31	22.205672929565278	27.403246198979826	28.945789975875275	21.44529089557962
32-33	21.64242619071595	27.772306260623846	28.9457837325429	21.639483816117306
34-35	21.868784492078614	27.78614254496951	28.927775368883484	21.41729759406839
36-37	21.83643909103411	27.70936989408054	28.86442423451257	21.58976678037278
38-39	21.71662563807945	27.66229269190105	28.959149544013247	21.661932126006253
40-41	21.983395499315176	27.480778669018203	28.865278156072367	21.670547675594253
42-43	21.955532489960223	27.5295927176348	28.68789322816615	21.82698156423883
44-45	21.981221018425316	27.71874492837101	28.4650166654315	21.83501738777218
46-47	22.146523495403912	27.646703187324434	28.08564867459958	22.121124642672076
48-49	22.04517562108386	27.745446305392452	28.125280276796843	22.084097796726844
50-51	22.037651813948887	27.91049315172446	28.007764457800615	22.044090576526035
52-53	22.16670738012056	27.912854899659408	27.98100295786856	21.939434762351475
54-55	22.151066019882574	27.77878750955651	27.88897267239296	22.18117379816796
56-57	22.230617469282862	28.001909081133213	27.728212967739026	22.039260481844895
58-59	22.08945711137831	28.099390632412945	27.87326073959765	21.937891516611092
60-61	22.227901050356667	28.120630978069055	27.727680044529755	21.923787927044522
62-63	22.085457867680265	28.229227412150195	27.699940488117065	21.985374232052482
64-65	22.0875200437779	28.11898460661964	27.66258311312465	22.130912236477805
66-67	22.1773743926275	28.1033105213015	27.582390838538394	22.13692424753261
68-69	22.171853119119024	28.023309846630745	27.662678963229116	22.142158071021118
70-71	22.177875733496315	27.805619215443084	27.710307051627286	22.30619799943332
72-73	22.2274471317946	27.85779653530697	27.652308421347506	22.26244791155092
74-75	22.127798003065926	27.983557960679693	27.612249302546793	22.276394733707583
76-77	22.206397095557612	27.96953331937102	27.543204477365492	22.28086510770588
78-79	22.782937084503722	27.857705107606417	27.090862561555056	22.268495246334805
80-81	22.227446943757982	27.973831874830847	27.50698869130495	22.29173249010622
82-83	22.232528203099577	27.93622245494025	27.58859328307606	22.242656058884112
84-85	22.33283836838008	27.930748780343023	27.523378375763556	22.21303447551334
86-87	22.20024148658311	27.964459599312296	27.573516515445235	22.261782398659356
88-89	22.267960937555685	27.902865980997998	27.559497081275257	22.26967600017106
90-91	22.284771979629483	27.92706922629593	27.553492647824307	22.234666146250284
92-93	22.27298445997279	27.908776366813647	27.570347539628457	22.247891633585105
94-95	22.201040739457014	27.97114533158457	27.595590157308493	22.232223771649924
96-97	22.257607259267154	28.095748038134804	27.617991487996363	22.028653214601675
98-99	23.344158547545106	30.006132207146614	27.831868698385254	18.817840546923026
100	22.243856995472854	31.057577625672344	21.753830705383642	24.94473467347116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1291.0
1	1148.0
2	1432.0
3	1974.5
4	2697.5
5	4354.5
6	5726.0
7	6710.5
8	7486.5
9	7631.5
10	7284.0
11	6978.0
12	7808.5
13	10313.0
14	15864.5
15	23155.0
16	29187.0
17	33090.0
18	34162.0
19	32337.5
20	29072.0
21	25622.0
22	23571.5
23	24471.5
24	28105.0
25	34571.5
26	43589.0
27	53928.5
28	64519.0
29	76331.5
30	88700.0
31	101065.0
32	114165.0
33	126795.0
34	139058.0
35	151053.0
36	164967.5
37	178892.5
38	188938.0
39	195982.5
40	203818.0
41	210208.5
42	212980.0
43	215799.0
44	219331.5
45	224891.5
46	229090.5
47	230724.5
48	229014.5
49	223034.5
50	216336.5
51	207509.5
52	196085.0
53	183526.0
54	170845.5
55	157604.0
56	143460.5
57	128669.5
58	113963.0
59	99177.5
60	82879.5
61	66953.0
62	52916.0
63	40682.5
64	30116.0
65	22037.5
66	16483.0
67	12521.5
68	9491.5
69	6864.5
70	4792.0
71	3298.5
72	2255.0
73	1574.0
74	1075.0
75	713.0
76	437.0
77	244.0
78	150.5
79	102.5
80	71.0
81	52.5
82	42.5
83	27.5
84	18.5
85	16.5
86	11.5
87	7.0
88	3.5
89	3.0
90	2.5
91	1.5
92	1.5
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	1.8211957315542921E-4
4	0.02536925654055129
5	0.01731957140708132
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008370380692929079
26-27	0.0512468471800796
28-29	0.08217517019265229
30-31	0.0722698761314573
32-33	0.033853257001211626
34-35	0.004091444049090738
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.1681554089958875E-4
56-57	7.806445430702086E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.0299562783559837E-5
72-73	0.013765839539726802
74-75	0.02680867521195805
76-77	7.280044532770963E-4
78-79	5.717890374746175
80-81	0.0
82-83	0.0122277550978854
84-85	0.04706200646083659
86-87	0.036366395069166724
88-89	0.008953161601894329
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	75.0
20-21	311.0
22-23	631.0
24-25	1276.0
26-27	2075.0
28-29	3277.0
30-31	7367.0
32-33	10101.0
34-35	14251.0
36-37	22538.0
38-39	28163.0
40-41	29103.0
42-43	32188.0
44-45	39750.0
46-47	39333.0
48-49	34357.0
50-51	30138.0
52-53	29753.0
54-55	33284.0
56-57	35679.0
58-59	36274.0
60-61	36222.0
62-63	37173.0
64-65	39801.0
66-67	42573.0
68-69	40851.0
70-71	38798.0
72-73	37648.0
74-75	39306.0
76-77	40723.0
78-79	40265.0
80-81	40942.0
82-83	40608.0
84-85	41063.0
86-87	43551.0
88-89	46604.0
90-91	48991.0
92-93	49892.0
94-95	59938.0
96-97	177312.0
98-99	342385.0
100-101	3776328.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.38365155935077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.57784609791635	80.9398444007409
2	6.265571094906186	11.075481049450564
3	1.311940633640625	3.478623114907405
4	0.41199989973646456	1.4565622232318052
5	0.18253648304375406	0.8066620457104249
6	0.08921005138973279	0.47308260585731504
7	0.04573974126222128	0.28298517478945173
8	0.03254911370550035	0.2301447619450105
9	0.018796213045204995	0.14951501499804493
>10	0.061687726855498275	0.8406782779753967
>50	0.001295387424006251	0.07363126628526656
>100	7.694691688046456E-4	0.1522569681906911
>500	2.904395682097036E-5	0.014785983127120166
>1k	2.904394877365812E-5	0.02574711279063588
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.4635871946628766E-5	0.0	0.0	0.0
3	0.0	5.4635871946628766E-5	0.0	0.0	0.0
4	0.0	1.0927174389325753E-4	0.0	0.0	1.821195731554292E-5
5	0.0	1.0927174389325753E-4	0.0	0.0	1.821195731554292E-5
6	3.642391463108584E-5	1.0927174389325753E-4	0.0	0.0	1.821195731554292E-5
7	3.642391463108584E-5	1.0927174389325753E-4	0.0	0.0	1.821195731554292E-5
8	3.642391463108584E-5	1.0927174389325753E-4	0.0	1.821195731554292E-5	1.821195731554292E-5
9	3.642391463108584E-5	1.639076158398863E-4	0.0	3.642391463108584E-5	1.821195731554292E-5
10-11	5.4635871946628766E-5	1.639076158398863E-4	0.0	7.284782926217168E-5	1.821195731554292E-5
12-13	5.4635871946628766E-5	1.639076158398863E-4	0.0	7.284782926217168E-5	1.821195731554292E-5
14-15	5.4635871946628766E-5	2.549674024176009E-4	0.0	1.0016576523548608E-4	3.642391463108584E-5
16-17	6.374185060440023E-5	2.7317935973314385E-4	0.0	1.0927174389325753E-4	3.642391463108584E-5
18-19	7.284782926217168E-5	3.0049729570645823E-4	0.0	1.365896798665719E-4	3.642391463108584E-5
20-21	7.284782926217168E-5	3.278152316797726E-4	0.0	1.5480163718211483E-4	4.5529893288857304E-5
22-23	9.105978657771461E-5	3.278152316797726E-4	0.0	1.639076158398863E-4	6.374185060440023E-5
24-25	9.105978657771461E-5	3.733451249686299E-4	0.0	1.639076158398863E-4	9.105978657771461E-5
26-27	9.105978657771461E-5	4.006630609419443E-4	0.0	2.3675544510205797E-4	9.105978657771461E-5
28-29	9.105978657771461E-5	4.006630609419443E-4	0.0	4.0976903959971575E-4	9.105978657771461E-5
30-31	9.105978657771461E-5	4.5529893288857306E-4	0.0	9.652337377237749E-4	1.0016576523548608E-4
32-33	9.105978657771461E-5	4.5529893288857306E-4	0.0	0.002149010963234065	1.0927174389325753E-4
34-35	9.105978657771461E-5	5.463587194662876E-4	0.0	0.003533119719215327	1.0927174389325753E-4
36-37	9.105978657771461E-5	6.374185060440022E-4	0.0	0.005946204063524764	1.2748370120880046E-4
38-39	9.105978657771461E-5	6.465244847017737E-4	0.0	0.00947021780408232	1.2748370120880046E-4
40-41	9.105978657771461E-5	6.920543779906311E-4	0.0	0.013094397309875362	1.2748370120880046E-4
42-43	9.105978657771461E-5	7.011603566484026E-4	0.0	0.017410631193659033	1.2748370120880046E-4
44-45	1.0016576523548608E-4	7.375842712794884E-4	0.0	0.02187256073596705	1.2748370120880046E-4
46-47	1.0927174389325753E-4	7.649022072528027E-4	0.0	0.026944590848345752	1.365896798665719E-4
48-49	1.0927174389325753E-4	7.649022072528027E-4	0.0	0.032207846512537656	1.4569565852434337E-4
50-51	1.0927174389325753E-4	8.286440578572029E-4	0.0	0.037707857621831625	1.4569565852434337E-4
52-53	1.0927174389325753E-4	8.377500365149744E-4	0.0	0.042962007307365754	1.5480163718211483E-4
54-55	1.0927174389325753E-4	8.377500365149744E-4	0.0	0.048698773861761774	1.639076158398863E-4
56-57	1.0927174389325753E-4	8.559619938305174E-4	0.0	0.052942159916283274	1.639076158398863E-4
58-59	1.0927174389325753E-4	8.559619938305174E-4	0.0	0.05725839380006695	1.639076158398863E-4
60-61	1.456956585243434E-4	8.832799298038316E-4	0.0	0.06221204618989462	1.639076158398863E-4
62-63	2.0033153047097215E-4	9.197038444349176E-4	0.0	0.0665282800736783	1.639076158398863E-4
64-65	2.094375091287436E-4	9.834456950393179E-4	0.0	0.07100842157330185	1.639076158398863E-4
66-67	2.36755445102058E-4	9.834456950393179E-4	0.0	0.07566157666742307	1.7301359449765777E-4
68-69	2.36755445102058E-4	9.834456950393179E-4	0.0	0.07943145183174045	1.8211957315542921E-4
70-71	2.36755445102058E-4	0.0010016576523548607	0.0	0.08269139219122264	1.8211957315542921E-4
72-73	2.36755445102058E-4	0.0010198696096704036	0.0	0.08512268849284763	1.8211957315542921E-4
74-75	2.36755445102058E-4	0.0010562935243014896	0.0	0.08769968045299695	1.8211957315542921E-4
76-77	2.4586142375982947E-4	0.0011109293962481182	0.0	0.0902675664344885	1.8211957315542921E-4
78-79	2.549674024176009E-4	0.0011291413535636612	0.0	0.09162435725449644	1.8211957315542921E-4
80-81	2.549674024176009E-4	0.0011291413535636612	0.0	0.0921433980379894	1.8211957315542921E-4
82-83	2.549674024176009E-4	0.0011291413535636612	0.0	0.09245300131235365	2.0033153047097215E-4
84-85	2.549674024176009E-4	0.0011655652681947471	0.0	0.09265333284282462	2.0033153047097215E-4
86-87	2.549674024176009E-4	0.0012202011401413757	0.0	0.09273528665074456	2.0033153047097215E-4
88	2.7317935973314385E-4	0.0012384130974569187	0.0	0.09273528665074456	2.0033153047097215E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7765	0.0	48.987747	1
GTATCAA	13845	0.0	36.66423	1
TCAACGC	16185	0.0	30.672905	4
ATCAACG	16255	0.0	30.593702	3
CAACGCA	16705	0.0	29.760893	5
TATCAAC	17115	0.0	29.433123	2
AACGCAG	17225	0.0	28.882658	6
ACGCAGA	19660	0.0	25.349087	7
CGCAGAG	19900	0.0	25.04337	8
CTCGTAT	4205	0.0	21.455624	9
GTGGTAT	3730	0.0	21.318102	1
GCAGAGT	23460	0.0	21.224785	9
CTTATAC	3585	0.0	21.22119	1
AATCTCG	4350	0.0	21.036728	6
TCTCGTA	4320	0.0	20.785015	8
GAGTACT	14345	0.0	20.335604	12-13
TGGTATC	3740	0.0	19.537228	2
GTACATG	15195	0.0	19.376507	1
TACATGG	14900	0.0	19.26974	2
ATCTCGT	4660	0.0	19.268513	7
>>END_MODULE