FastQCFastQC Report
Fri 27 May 2016
SRR2138881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421055
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81750.050.987251
GTATCAA147350.037.376141
TCAACGC176100.030.6872774
ATCAACG176350.030.6437723
CAACGCA179950.029.9983885
TATCAAC184650.029.6161382
AACGCAG186350.029.1040086
ACGCAGA214450.025.230217
CGCAGAG217350.024.8737728
CTTATAC42950.022.5576211
GCAGAGT259350.020.8290339
GTGGTAT41150.020.4051021
TGGTATC37550.020.182582
GTACATG178000.020.1027281
GAGTACT158050.019.99001712-13
TATACAC63000.019.616223
TACATGG175550.019.5002542
ACATGGG177000.019.1215573
CAGAGTA250050.018.91831610-11
AGAGTAC230300.018.718410-11
Produced by FastQC (version 0.11.5)