##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138881_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6421055
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84523758167466	33.0	33.0	33.0	33.0	33.0
2	32.84277879569635	33.0	33.0	33.0	33.0	33.0
3	32.84409555750574	33.0	33.0	33.0	33.0	33.0
4	36.794604624940916	37.0	37.0	37.0	37.0	37.0
5	36.80329307255583	37.0	37.0	37.0	37.0	37.0
6	36.81405703579863	37.0	37.0	37.0	37.0	37.0
7	36.799669991925	37.0	37.0	37.0	37.0	37.0
8	36.79794800075688	37.0	37.0	37.0	37.0	37.0
9	36.797204042014904	37.0	37.0	37.0	37.0	37.0
10-11	36.777880581929296	37.0	37.0	37.0	37.0	37.0
12-13	36.76674635242962	37.0	37.0	37.0	37.0	37.0
14-15	39.1556155491582	40.0	40.0	40.0	37.0	40.0
16-17	39.11778858770093	40.0	40.0	40.0	37.0	40.0
18-19	39.089756434106235	40.0	40.0	40.0	37.0	40.0
20-21	39.13714532721221	40.0	40.0	40.0	37.0	40.0
22-23	39.17141756699908	40.0	40.0	40.0	37.0	40.0
24-25	39.17715755827354	40.0	40.0	40.0	37.0	40.0
26-27	38.92037392560441	40.0	40.0	40.0	37.0	40.0
28-29	38.92723472557212	40.0	40.0	40.0	37.0	40.0
30-31	38.94982188049849	40.0	40.0	40.0	37.0	40.0
32-33	38.93960711176493	40.0	40.0	40.0	37.0	40.0
34-35	38.92141342893831	40.0	40.0	40.0	37.0	40.0
36-37	38.890917755634916	40.0	40.0	40.0	37.0	40.0
38-39	38.85136099619497	40.0	40.0	40.0	37.0	40.0
40-41	38.808674920257204	40.0	40.0	40.0	37.0	40.0
42-43	38.756360934380616	40.0	37.0	40.0	37.0	40.0
44-45	38.69133995191298	40.0	37.0	40.0	37.0	40.0
46-47	38.62306526699014	40.0	37.0	40.0	37.0	40.0
48-49	38.56933753794394	40.0	37.0	40.0	37.0	40.0
50-51	38.515719216723134	40.0	37.0	40.0	37.0	40.0
52-53	38.449186871953394	40.0	37.0	40.0	37.0	40.0
54-55	38.378557211255895	40.0	37.0	40.0	37.0	40.0
56-57	38.27977480460021	40.0	37.0	40.0	37.0	40.0
58-59	38.14801578945471	40.0	37.0	40.0	37.0	40.0
60-61	37.99329905403986	40.0	37.0	40.0	37.0	40.0
62-63	37.80981791371078	40.0	37.0	40.0	33.0	40.0
64-65	37.59757059011597	37.0	37.0	40.0	33.0	40.0
66-67	37.37366918567649	37.0	37.0	40.0	33.0	40.0
68-69	37.11676170260135	37.0	37.0	40.0	33.0	40.0
70-71	36.863120453048275	37.0	37.0	40.0	33.0	40.0
72-73	36.61877703437418	37.0	37.0	38.5	33.0	40.0
74-75	36.35301829715201	37.0	37.0	37.0	33.0	40.0
76-77	34.65636002685416	35.0	33.0	37.0	30.0	37.0
78-79	31.471273757718055	37.0	33.0	37.0	8.5	37.0
80-81	34.586372453719065	37.0	33.0	37.0	30.0	37.0
82-83	35.62618762204022	37.0	35.0	37.0	33.0	37.0
84-85	35.748260031167376	37.0	37.0	37.0	33.0	37.0
86-87	35.708144953343904	37.0	37.0	37.0	33.0	37.0
88-89	35.62907262655381	37.0	37.0	37.0	33.0	37.0
90-91	35.56344633554655	37.0	37.0	37.0	33.0	37.0
92-93	35.49174332878823	37.0	33.0	37.0	33.0	37.0
94-95	35.44538546952067	37.0	33.0	37.0	33.0	37.0
96-97	35.42042214541511	37.0	33.0	37.0	33.0	37.0
98-99	35.38069110722571	37.0	33.0	37.0	33.0	37.0
100	35.333599181375014	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	16.0
21	79.0
22	297.0
23	932.0
24	2371.0
25	5064.0
26	9255.0
27	15449.0
28	23058.0
29	33501.0
30	46545.0
31	63217.0
32	85870.0
33	119099.0
34	173440.0
35	283852.0
36	633153.0
37	2037620.0
38	2762406.0
39	125830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56136335851352	20.18534337425859	14.465146303839477	23.788146963388414
2	14.367311913696426	21.04780289220385	41.85914931424821	22.72573587985152
3	17.80273186402757	26.229579427746643	30.829272592573442	25.138416115652344
4	12.28078866000981	18.343475014670037	39.191456967290684	30.18427935802947
5	13.29421083005387	37.635469131254574	35.66403764483049	13.406282393861064
6	29.48988912258188	38.07838431535005	19.028368391175594	13.40335817089248
7	26.120676430898037	32.949414076035794	23.18156440024264	17.74834509282353
8	24.249846793089297	36.39401936286171	21.54228549669797	17.81384834735102
9	25.476171750592385	16.95816341707087	21.344202782876025	36.22146204946072
10-11	23.787446143974783	26.848866736073745	29.20312939228834	20.160557727663132
12-13	24.81369494576826	24.460879092298697	29.139845399237352	21.585580562695693
14-15	22.039594739493744	25.85076751406116	26.861395829813013	25.24824191663208
16-17	21.544021971467306	29.013518806488964	27.503587806053677	21.938871415990054
18-19	21.608240390403136	28.065894156022647	29.19704783715449	21.12881761641973
20-21	22.330761855874332	27.242883921262766	29.221437880239563	21.204916342623335
22-23	21.982939143016676	27.27638385837962	29.052962014906836	21.687714983696868
24-25	21.837031864919236	27.403017338745983	29.114887068677316	21.645063727657462
26-27	21.879010171800893	27.542613099365155	28.9874928823896	21.59088384644435
28-29	21.631712407045388	27.55128236430524	29.087106947405307	21.729898281244065
30-31	22.039641384239413	27.35767896578353	29.075094473738094	21.52758517623896
32-33	21.77238595592705	27.586349712104457	28.943717228697828	21.69754710327066
34-35	21.86881874061376	27.663934698897158	28.97142356651529	21.495822993973785
36-37	21.997179174677463	27.52897757992463	28.78101999417501	21.692823251222897
38-39	21.76019319750939	27.614944441927484	28.893806373075265	21.73105598748786
40-41	22.031754318989957	27.497943394653802	28.77539352538765	21.694908760968595
42-43	22.001150234482196	27.527645843614412	28.60472770398384	21.866476217919555
44-45	21.974814709247443	27.72347581992709	28.41724026668097	21.88446920414449
46-47	22.133261100372785	27.676022265519695	28.056882811109784	22.133833822997733
48-49	22.083873125526168	27.730502267309998	28.08700679742128	22.098617809742557
50-51	22.05909582857447	27.90836364004981	27.98025446605611	22.05228606531961
52-53	22.195582037872146	27.89142611977986	27.90964669031065	22.00334515203734
54-55	22.149464771067127	27.805329370028588	27.880778614365774	22.164427244538512
56-57	22.21878441593563	27.971199250506423	27.727515588152546	22.0825007454054
58-59	22.09477469712126	28.05713212464124	27.867359695599426	21.980733482638083
60-61	22.266302617047888	28.08926393960499	27.68501583828657	21.95941760506055
62-63	22.118383083148856	28.20502155222827	27.651685369365005	22.02490999525787
64-65	22.140713916990546	28.119042560294226	27.648031194603817	22.09221232811141
66-67	22.220474649718057	28.03793803228886	27.58938322049824	22.15220409749484
68-69	22.216436590473556	27.975494527696913	27.6536656141543	22.154403267675228
70-71	22.218177151817294	27.774641920445514	27.69375320812561	22.31342771961158
72-73	22.241208557692683	27.805943681032876	27.634464077278274	22.31838368399617
74-75	22.116730553952753	27.973215490927096	27.596413164166695	22.313640790953457
76-77	22.23579659584608	27.907684413284024	27.532828175716755	22.323690815153142
78-79	22.736294533959015	27.862401531290715	27.1145850141526	22.286718920597675
80-81	22.217670815441952	27.951334525924093	27.53104112880264	22.29995352983131
82-83	22.24461318378296	27.919114364772348	27.568086643680967	22.268185807763725
84-85	22.33508190392774	27.905708232692987	27.538372136218637	22.22083772716064
86-87	22.197832419893064	27.8901931457602	27.622838800946	22.28913563340074
88-89	22.2683329277886	27.90220029649932	27.552813762543245	22.27665301316884
90-91	22.29402022660283	27.935011670672193	27.52540432258791	22.245563780137065
92-93	22.291059271413282	27.900020053273305	27.552051069002694	22.25686960631072
94-95	22.19005617532877	27.979118725257067	27.559143374167412	22.271681725246747
96-97	22.283175927373158	28.079791286726852	27.628708535122065	22.00832425077793
98-99	23.387181237889678	29.980167366286985	27.822259221137053	18.81039217468628
100	22.311014161271295	30.989678521408383	21.716633431891633	24.982673885428692
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1350.0
1	1197.5
2	1579.0
3	2283.0
4	3132.0
5	4935.0
6	6396.5
7	7410.5
8	8154.0
9	8216.0
10	7862.5
11	7597.5
12	8452.0
13	11042.0
14	17021.5
15	25028.5
16	31829.5
17	36191.0
18	37177.5
19	35297.0
20	31818.5
21	28019.5
22	26104.5
23	27400.5
24	31911.5
25	39810.5
26	50718.5
27	63339.0
28	76177.0
29	90147.0
30	104742.0
31	119028.5
32	133712.0
33	148562.0
34	164084.5
35	178474.5
36	192990.0
37	206873.0
38	217232.0
39	226282.5
40	236305.0
41	245265.5
42	251354.0
43	256676.5
44	260282.5
45	264182.0
46	267880.5
47	269796.5
48	267915.0
49	261786.0
50	254349.5
51	242953.0
52	229034.0
53	213650.0
54	197899.5
55	182544.0
56	166746.0
57	150349.0
58	133280.0
59	115478.0
60	96493.5
61	78217.0
62	61687.0
63	47373.0
64	35162.5
65	25823.0
66	19404.0
67	14745.0
68	11043.0
69	7919.5
70	5611.5
71	3963.5
72	2759.5
73	1885.5
74	1239.5
75	780.5
76	503.5
77	332.5
78	213.5
79	137.5
80	96.5
81	60.5
82	37.0
83	26.0
84	22.0
85	18.0
86	13.5
87	10.5
88	7.5
89	5.0
90	3.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	2.803277654528734E-4
4	0.023485237239051836
5	0.016352452984750947
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008505229314067275
26-27	0.05197196645685189
28-29	0.08431550084132342
30-31	0.07442136046708628
32-33	0.0359647236367991
34-35	0.0041951847262716325
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.1355477040803875E-4
56-57	8.575939444209184E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.013299398160146551
74-75	0.026828765759778765
76-77	8.194988125372151E-4
78-79	5.024848947757799
80-81	0.0
82-83	0.01198370327315067
84-85	0.04804532941557101
86-87	0.037434050260673174
88-89	0.009175023702936883
90-91	4.804325122517497E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	53.0
20-21	311.0
22-23	733.0
24-25	1533.0
26-27	2392.0
28-29	4008.0
30-31	8647.0
32-33	11591.0
34-35	15682.0
36-37	23246.0
38-39	28509.0
40-41	30953.0
42-43	37376.0
44-45	45975.0
46-47	44936.0
48-49	39832.0
50-51	35679.0
52-53	35100.0
54-55	38882.0
56-57	41708.0
58-59	42589.0
60-61	42635.0
62-63	43637.0
64-65	46469.0
66-67	49941.0
68-69	48136.0
70-71	46232.0
72-73	44535.0
74-75	46177.0
76-77	47611.0
78-79	47478.0
80-81	49151.0
82-83	47767.0
84-85	48459.0
86-87	50862.0
88-89	54322.0
90-91	57082.0
92-93	59185.0
94-95	71056.0
96-97	208199.0
98-99	401314.0
100-101	4421072.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.93036982821788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.19019299531516	80.18387394784625
2	6.511651002993054	11.451437617709304
3	1.3751615672567739	3.627553955473195
4	0.4548363621724921	1.5997571814859395
5	0.19565384207136574	0.8601957345823462
6	0.09496823657158311	0.5010355298203794
7	0.05555638564951063	0.34195654755363963
8	0.03144126483828741	0.2211713636078044
9	0.02249326294375176	0.17800568363586972
>10	0.0665942129167435	0.88031770231804
>50	9.846859865040316E-4	0.057263480105221774
>100	4.4104797978229265E-4	0.0835083768109067
>500	2.513330497861056E-5	0.013922879051180085
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5573764747381857E-5
2	0.0	0.0	0.0	0.0	1.5573764747381857E-5
3	0.0	0.0	0.0	0.0	1.5573764747381857E-5
4	0.0	0.0	0.0	0.0	3.1147529494763714E-5
5	0.0	1.5573764747381857E-5	0.0	0.0	3.1147529494763714E-5
6	3.1147529494763714E-5	1.5573764747381857E-5	0.0	0.0	3.1147529494763714E-5
7	3.1147529494763714E-5	3.1147529494763714E-5	0.0	4.6721294242145564E-5	3.1147529494763714E-5
8	3.1147529494763714E-5	6.229505898952743E-5	0.0	4.6721294242145564E-5	4.6721294242145564E-5
9	3.1147529494763714E-5	1.0901635323167298E-4	0.0	4.6721294242145564E-5	6.229505898952743E-5
10-11	4.6721294242145564E-5	1.0901635323167298E-4	0.0	9.344258848429113E-5	6.229505898952743E-5
12-13	7.786882373690927E-5	1.0901635323167298E-4	0.0	9.344258848429113E-5	6.229505898952743E-5
14-15	8.56557061106002E-5	1.2459011797905485E-4	0.0	9.344258848429113E-5	6.229505898952743E-5
16-17	1.1680323560536393E-4	1.401638827264367E-4	0.0	1.0901635323167298E-4	7.786882373690927E-5
18-19	1.2459011797905485E-4	1.6352452984750947E-4	0.0	1.0901635323167298E-4	7.786882373690927E-5
20-21	1.2459011797905485E-4	1.8688517696858226E-4	0.0	1.2459011797905485E-4	7.786882373690927E-5
22-23	1.3237700035274578E-4	2.1024582408965504E-4	0.0	1.401638827264367E-4	9.344258848429113E-5
24-25	1.7909829459489133E-4	2.4139335358441875E-4	0.0	1.713114122212004E-4	1.0901635323167298E-4
26-27	2.024589417159641E-4	2.491802359581097E-4	0.0	2.1803270646334597E-4	1.0901635323167298E-4
28-29	2.024589417159641E-4	2.491802359581097E-4	0.0	3.659834715634736E-4	1.0901635323167298E-4
30-31	2.1024582408965504E-4	2.803277654528734E-4	0.0	5.995899427742014E-4	1.0901635323167298E-4
32-33	2.3360647121072783E-4	2.959015302002553E-4	0.0	0.0012147536502957847	1.0901635323167298E-4
34-35	2.4139335358441875E-4	3.5040970681609175E-4	0.0	0.002297130300238824	1.0901635323167298E-4
36-37	2.491802359581097E-4	4.127047658056192E-4	0.0	0.003994670657703446	1.0901635323167298E-4
38-39	2.491802359581097E-4	4.28278530553001E-4	0.0	0.0068991777830901616	1.0901635323167298E-4
40-41	2.491802359581097E-4	4.3606541292669194E-4	0.0	0.010200815909535116	1.0901635323167298E-4
42-43	2.491802359581097E-4	4.438522953003829E-4	0.0	0.013292208211890415	1.0901635323167298E-4
44-45	2.6475400070549157E-4	5.061473542899103E-4	0.0	0.01628237104338773	1.0901635323167298E-4
46-47	2.803277654528734E-4	5.606555309057468E-4	0.0	0.01997335328851723	1.0901635323167298E-4
48-49	2.803277654528734E-4	5.606555309057468E-4	0.0	0.023882368240110075	1.0901635323167298E-4
50-51	2.881146478265643E-4	6.229505898952742E-4	0.0	0.027908186427308287	1.0901635323167298E-4
52-53	3.114752949476371E-4	6.229505898952742E-4	0.0	0.03163810308430624	1.3237700035274576E-4
54-55	3.114752949476371E-4	6.307374722689652E-4	0.0	0.03574957697761505	1.5573764747381854E-4
56-57	3.114752949476371E-4	6.540981193900379E-4	0.0	0.04035162446046639	1.5573764747381854E-4
58-59	3.114752949476371E-4	6.774587665111107E-4	0.0	0.044852442472459744	1.5573764747381854E-4
60-61	3.114752949476371E-4	7.008194136321835E-4	0.0	0.04912744089561606	1.5573764747381854E-4
62-63	3.426228244424008E-4	7.008194136321835E-4	0.0	0.05396309484967813	1.5573764747381854E-4
64-65	3.426228244424008E-4	7.942620021164746E-4	0.0	0.058526207920661016	1.5573764747381854E-4
66-67	3.5819658918978265E-4	8.254095316112384E-4	0.0	0.06299587840315961	1.5573764747381854E-4
68-69	3.5819658918978265E-4	8.409832963586202E-4	0.0	0.06718522112020534	1.5573764747381854E-4
70-71	3.5819658918978265E-4	8.565570611060021E-4	0.0	0.07109423607179817	1.5573764747381854E-4
72-73	3.5819658918978265E-4	8.565570611060021E-4	0.0	0.07407661202092179	1.713114122212004E-4
74-75	3.5819658918978265E-4	8.799177082270749E-4	0.0	0.07659177502762396	1.713114122212004E-4
76-77	3.5819658918978265E-4	9.422127672166023E-4	0.0	0.0787876758570048	1.713114122212004E-4
78-79	3.5819658918978265E-4	9.499996495902932E-4	0.0	0.08023603597851131	1.713114122212004E-4
80-81	3.5819658918978265E-4	9.577865319639841E-4	0.0	0.08082783903891183	1.713114122212004E-4
82-83	3.6598347156347355E-4	9.65573414337675E-4	0.0	0.08121718315759638	1.713114122212004E-4
84-85	3.737703539371645E-4	9.65573414337675E-4	0.0	0.08138849456981757	1.713114122212004E-4
86-87	3.737703539371645E-4	9.811471790850568E-4	0.0	0.08147415027592816	1.713114122212004E-4
88	3.737703539371645E-4	9.811471790850568E-4	0.0	0.08149751092304924	1.713114122212004E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8175	0.0	50.98725	1
GTATCAA	14735	0.0	37.37614	1
TCAACGC	17610	0.0	30.687277	4
ATCAACG	17635	0.0	30.643772	3
CAACGCA	17995	0.0	29.998388	5
TATCAAC	18465	0.0	29.616138	2
AACGCAG	18635	0.0	29.104008	6
ACGCAGA	21445	0.0	25.23021	7
CGCAGAG	21735	0.0	24.873772	8
CTTATAC	4295	0.0	22.557621	1
GCAGAGT	25935	0.0	20.829033	9
GTGGTAT	4115	0.0	20.405102	1
TGGTATC	3755	0.0	20.18258	2
GTACATG	17800	0.0	20.102728	1
GAGTACT	15805	0.0	19.990017	12-13
TATACAC	6300	0.0	19.61622	3
TACATGG	17555	0.0	19.500254	2
ACATGGG	17700	0.0	19.121557	3
CAGAGTA	25005	0.0	18.918316	10-11
AGAGTAC	23030	0.0	18.7184	10-11
>>END_MODULE