FastQCFastQC Report
Fri 27 May 2016
SRR2138880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6228226
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.050.3789631
GTATCAA140250.037.4294241
TCAACGC163250.031.2599074
ATCAACG163650.031.157213
CAACGCA166700.030.580765
TATCAAC173350.029.711632
AACGCAG172900.029.4799086
ACGCAGA195500.026.1160017
CGCAGAG199750.025.5603458
GTACATG162650.021.534091
GCAGAGT238400.021.4164379
TACATGG160000.021.0301782
ACATGGG161800.020.1845683
GTGGTAT37900.020.0951041
GAGTACT147750.020.07287212-13
CAGAGTA231800.019.06650710-11
AGAGTAC212900.019.04181910-11
CATGGGG119450.017.9390474
TGGTATC36550.017.6587542
GTACTTT169450.017.40098814-15
Produced by FastQC (version 0.11.5)