##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6228226
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84656786699776	33.0	33.0	33.0	33.0	33.0
2	32.84166085174173	33.0	33.0	33.0	33.0	33.0
3	32.84247296100045	33.0	33.0	33.0	33.0	33.0
4	36.7928390845162	37.0	37.0	37.0	37.0	37.0
5	36.803714733537284	37.0	37.0	37.0	37.0	37.0
6	36.814521984269675	37.0	37.0	37.0	37.0	37.0
7	36.800748078184704	37.0	37.0	37.0	37.0	37.0
8	36.79806272283633	37.0	37.0	37.0	37.0	37.0
9	36.7976992164382	37.0	37.0	37.0	37.0	37.0
10-11	36.77884384734915	37.0	37.0	37.0	37.0	37.0
12-13	36.76735301512822	37.0	37.0	37.0	37.0	37.0
14-15	39.15695143368272	40.0	40.0	40.0	37.0	40.0
16-17	39.12236750882194	40.0	40.0	40.0	37.0	40.0
18-19	39.09396712643375	40.0	40.0	40.0	37.0	40.0
20-21	39.1432648923205	40.0	40.0	40.0	37.0	40.0
22-23	39.174325863411354	40.0	40.0	40.0	37.0	40.0
24-25	39.18046834892477	40.0	40.0	40.0	37.0	40.0
26-27	38.92355176243731	40.0	40.0	40.0	37.0	40.0
28-29	38.93412074891861	40.0	40.0	40.0	37.0	40.0
30-31	38.95741955711874	40.0	40.0	40.0	37.0	40.0
32-33	38.95215077330198	40.0	40.0	40.0	37.0	40.0
34-35	38.93497224849786	40.0	40.0	40.0	37.0	40.0
36-37	38.90594379862922	40.0	40.0	40.0	37.0	40.0
38-39	38.87502207985905	40.0	40.0	40.0	37.0	40.0
40-41	38.82901622946444	40.0	40.0	40.0	37.0	40.0
42-43	38.77464437052494	40.0	37.0	40.0	37.0	40.0
44-45	38.71403160066015	40.0	37.0	40.0	37.0	40.0
46-47	38.6470110575545	40.0	37.0	40.0	37.0	40.0
48-49	38.59744021063551	40.0	37.0	40.0	37.0	40.0
50-51	38.55702716744017	40.0	37.0	40.0	37.0	40.0
52-53	38.49240555075963	40.0	37.0	40.0	37.0	40.0
54-55	38.423275886354716	40.0	37.0	40.0	37.0	40.0
56-57	38.32834674879952	40.0	37.0	40.0	37.0	40.0
58-59	38.200767966924815	40.0	37.0	40.0	37.0	40.0
60-61	38.05153446477367	40.0	37.0	40.0	37.0	40.0
62-63	37.87067843283065	40.0	37.0	40.0	35.0	40.0
64-65	37.65822289104115	37.0	37.0	40.0	33.0	40.0
66-67	37.43435057227577	37.0	37.0	40.0	33.0	40.0
68-69	37.17817728883733	37.0	37.0	40.0	33.0	40.0
70-71	36.92285511768182	37.0	37.0	40.0	33.0	40.0
72-73	36.675949567502556	37.0	37.0	38.5	33.0	40.0
74-75	36.405323635495876	37.0	37.0	37.0	33.0	40.0
76-77	34.71411747246371	37.0	33.0	37.0	30.0	37.0
78-79	31.434931091515217	37.0	33.0	37.0	8.5	37.0
80-81	34.6060545301901	37.0	33.0	37.0	30.0	37.0
82-83	35.658867785360385	37.0	35.0	37.0	33.0	37.0
84-85	35.77726852674162	37.0	37.0	37.0	33.0	37.0
86-87	35.7383438428024	37.0	37.0	37.0	33.0	37.0
88-89	35.65848893362167	37.0	37.0	37.0	33.0	37.0
90-91	35.58876259474998	37.0	37.0	37.0	33.0	37.0
92-93	35.520250075246054	37.0	35.0	37.0	33.0	37.0
94-95	35.47531472814978	37.0	33.0	37.0	33.0	37.0
96-97	35.44931225071235	37.0	33.0	37.0	33.0	37.0
98-99	35.409725644975666	37.0	33.0	37.0	33.0	37.0
100	35.35829217122593	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	14.0
21	65.0
22	289.0
23	847.0
24	2250.0
25	4640.0
26	8451.0
27	14260.0
28	21760.0
29	31627.0
30	44078.0
31	60302.0
32	82228.0
33	113772.0
34	166173.0
35	269804.0
36	598190.0
37	1942828.0
38	2732684.0
39	133959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.612298590320904	20.461010888172652	14.166281056596212	23.760409464910232
2	14.215974179485457	21.38414694649809	42.01822477219035	22.381654101826108
3	17.476840850530362	26.809252356376025	31.05342660360733	24.660480189486286
4	11.984354087379042	18.51287386366228	40.04705786373165	29.455714185227023
5	12.90011560630521	38.10808731865154	36.03102790369107	12.960769171352183
6	28.886893314404453	38.75183720051263	19.417326860007968	12.943942625074941
7	25.62073052583513	33.19307937765906	23.98246306412131	17.203727032384503
8	23.94686705331502	36.6276689381535	21.908437490868184	17.517026517663297
9	25.535473504012216	16.739854976360846	21.706951546074276	36.017719973552666
10-11	23.555198542891667	26.94280040576562	29.791420542542934	19.710580508799776
12-13	24.64807795991989	24.665651182214646	29.582300000032113	21.103970857833353
14-15	21.835671987496923	26.00395361375775	27.31435082798858	24.846023570756746
16-17	21.316142028243675	28.997815750423957	27.915228188572478	21.77081403275989
18-19	21.334518047354095	28.269221444436987	29.576142548456012	20.820117959752906
20-21	22.157478123979676	27.51181558384611	29.433289507835404	20.89741678433881
22-23	21.80884749708964	27.430765525269962	29.294191321103124	21.466195656537273
24-25	21.598656035513557	27.517191037131678	29.391313216159375	21.492839711195394
26-27	21.688468840792723	27.673602399768686	29.190647022396416	21.44728173704217
28-29	21.539220325363566	27.766897941813106	29.248189038079175	21.44569269474415
30-31	21.846516700813464	27.60147851512093	29.153064749778512	21.398940034287094
32-33	21.630216881244927	27.79189931487272	29.060710829625624	21.51717297425673
34-35	21.746708597106593	27.79226582857431	29.042601916564966	21.418423657754136
36-37	21.771977860815856	27.720975333936256	29.009272075969672	21.497774729278216
38-39	21.633137973258986	27.857848879316442	29.05763047724395	21.451382670180617
40-41	21.906798566207133	27.74066287044914	28.870647324143334	21.481891239200397
42-43	21.814979929755374	27.840246071159974	28.68974229251243	21.65503170657222
44-45	21.909099310165193	27.95245226194646	28.48525304775246	21.653195380135887
46-47	21.915195044972098	27.94141119564814	28.212620215332805	21.930773544046957
48-49	21.882263802833915	28.00731654380072	28.234904346979018	21.87551530638635
50-51	21.912951720118613	28.173670894151797	28.05761709791752	21.855760287812064
52-53	22.054961914758373	28.101251378895707	27.9551370397133	21.88864966663262
54-55	22.054812937912928	27.97433143057849	28.003467257344088	21.96738837416449
56-57	22.051761214482656	28.086450645819923	27.954774031820456	21.907014107876968
58-59	22.007937949741674	28.115185476579185	27.97583493858203	21.901041635097112
60-61	22.06750229844379	28.117934280292754	27.940617038188094	21.87394638307536
62-63	22.00735782046198	28.185274433879528	27.904584606371536	21.902783139286957
64-65	22.01486424132108	28.10703761158708	27.878644140006315	21.99945400708552
66-67	22.104249659767103	28.120241207675022	27.746334902560164	22.029174229997707
68-69	22.036050452407828	28.130509284468502	27.812263221810905	22.021177041312765
70-71	22.05828445000352	27.91427007776534	27.878274233056054	22.14917123917508
72-73	22.113894033845664	27.961660284787637	27.8035322660766	22.120913415290094
74-75	21.978018027915823	28.095956926778438	27.752156804046002	22.17386824125974
76-77	22.11277644885313	28.02307124708891	27.706536264773835	22.15761603928413
78-79	22.598094046866294	27.980228498531684	27.293400677300628	22.128276777301394
80-81	22.06906930070847	28.085235664431302	27.66145186836921	22.184243166491022
82-83	22.112773485160453	28.06834131291986	27.73834854566422	22.080536656255465
84-85	22.21743645030819	28.037117310813347	27.6677500410944	22.077696197784068
86-87	22.04835742167758	28.023215254971092	27.76016862717374	22.16825869617759
88-89	22.13871440799577	28.010018315248864	27.728051597391808	22.12321567936356
90-91	22.158164981759096	28.04564838917746	27.7356584023879	22.060528226675544
92-93	22.15708275769336	28.026553810558102	27.718436198272983	22.097927233475552
94-95	22.04760390578919	28.086567106735373	27.7520024851801	22.11382650229534
96-97	22.114777516128445	28.231509668780667	27.79871801033082	21.854994804760068
98-99	23.24868993516995	30.11669543351389	28.011884427335303	18.62273020398086
100	22.126608577220722	31.10930328025289	21.932641439932596	24.831446702593794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1074.0
1	1045.0
2	1612.5
3	2392.5
4	3357.0
5	5411.0
6	7045.0
7	8090.5
8	8718.5
9	8615.5
10	7996.5
11	7373.0
12	8150.0
13	10728.5
14	16524.0
15	24231.5
16	30424.0
17	34232.5
18	35081.0
19	33242.5
20	30230.0
21	27342.0
22	26222.0
23	28250.5
24	33334.5
25	41661.5
26	53350.0
27	66465.5
28	79842.5
29	94034.0
30	108464.0
31	122866.5
32	138179.5
33	153740.0
34	168992.5
35	182692.0
36	197227.5
37	210377.0
38	218817.5
39	226012.5
40	234678.5
41	242494.0
42	246621.0
43	250107.0
44	252748.5
45	254717.0
46	254966.0
47	253428.0
48	251432.5
49	246029.5
50	238059.0
51	228523.5
52	216297.5
53	203003.5
54	189359.5
55	173735.0
56	157440.0
57	140993.5
58	124372.5
59	107193.0
60	88354.0
61	70384.0
62	54863.5
63	41639.5
64	30305.0
65	21912.0
66	16279.0
67	12199.0
68	9199.5
69	6595.0
70	4521.0
71	3140.0
72	2120.0
73	1337.5
74	859.0
75	570.0
76	361.0
77	224.5
78	133.5
79	81.0
80	52.0
81	31.0
82	24.0
83	19.5
84	16.5
85	11.0
86	9.0
87	9.5
88	8.0
89	6.5
90	3.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	2.890068536369746E-4
4	0.02286365330994733
5	0.01697112468301568
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008623884917226765
26-27	0.05305020187589691
28-29	0.08549296820336527
30-31	0.07568555050100666
32-33	0.03585312395710253
34-35	0.004478282869809539
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.612510666430916E-4
56-57	9.028877186890793E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.013415455170949728
74-75	0.02810368842021151
76-77	8.363791473950972E-4
78-79	5.113448598064234
80-81	0.0
82-83	0.01276316138922044
84-85	0.048758932348114285
86-87	0.03840078682190124
88-89	0.00968576716340066
90-91	1.9883257442253553E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	273.0
22-23	645.0
24-25	1475.0
26-27	2240.0
28-29	3699.0
30-31	8579.0
32-33	11210.0
34-35	15453.0
36-37	22838.0
38-39	28669.0
40-41	30798.0
42-43	36804.0
44-45	45486.0
46-47	45258.0
48-49	40633.0
50-51	36218.0
52-53	34876.0
54-55	38350.0
56-57	40247.0
58-59	40779.0
60-61	41010.0
62-63	40158.0
64-65	42267.0
66-67	45132.0
68-69	46019.0
70-71	46492.0
72-73	45037.0
74-75	45721.0
76-77	47915.0
78-79	48649.0
80-81	49369.0
82-83	47992.0
84-85	48635.0
86-87	51183.0
88-89	54357.0
90-91	57384.0
92-93	59439.0
94-95	69464.0
96-97	200698.0
98-99	386390.0
100-101	4270311.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.07968043989905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.38656377369016	78.70833089473493
2	7.079439553949121	12.329506681029418
3	1.5652390609969937	4.089015517309976
4	0.4950436324790759	1.7243296528033902
5	0.2044903485813754	0.8903477103754868
6	0.09907445660383106	0.5176423212490953
7	0.050280690875108544	0.306489854559126
8	0.03198351907823995	0.22280916965412398
9	0.019446913354587106	0.15240879004138652
>10	0.0668680502486724	0.8838700107992346
>50	0.0011093658567020634	0.06511330241195654
>100	4.078132793932819E-4	0.07283304456416458
>500	5.282100675725793E-5	0.037303050467722317
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6055936313165257E-5
2	0.0	0.0	0.0	1.6055936313165257E-5	1.6055936313165257E-5
3	0.0	0.0	0.0	3.2111872626330514E-5	1.6055936313165257E-5
4	0.0	0.0	0.0	4.8167808939495774E-5	1.6055936313165257E-5
5	0.0	0.0	0.0	4.8167808939495774E-5	1.6055936313165257E-5
6	0.0	0.0	0.0	4.8167808939495774E-5	1.6055936313165257E-5
7	0.0	0.0	0.0	6.422374525266103E-5	1.6055936313165257E-5
8	1.6055936313165257E-5	0.0	0.0	6.422374525266103E-5	3.2111872626330514E-5
9	1.6055936313165257E-5	8.027968156582629E-5	0.0	9.633561787899155E-5	3.2111872626330514E-5
10-11	1.6055936313165257E-5	8.027968156582629E-5	0.0	1.1239155419215681E-4	3.2111872626330514E-5
12-13	1.6055936313165257E-5	9.633561787899155E-5	0.0	1.5253139497506995E-4	3.2111872626330514E-5
14-15	1.6055936313165257E-5	1.2844749050532205E-4	0.0	1.685873312882352E-4	4.0139840782913144E-5
16-17	1.6055936313165257E-5	1.2844749050532205E-4	0.0	2.2478310838431361E-4	4.8167808939495774E-5
18-19	1.6055936313165257E-5	1.3647545866190468E-4	0.0	2.2478310838431361E-4	5.6195777096078404E-5
20-21	1.6055936313165257E-5	1.4450342681848733E-4	0.0	2.2478310838431361E-4	6.422374525266103E-5
22-23	1.6055936313165257E-5	1.7661529944481782E-4	0.0	2.3281107654089624E-4	6.422374525266103E-5
24-25	1.6055936313165257E-5	2.0069920391456572E-4	0.0	2.568949810106441E-4	6.422374525266103E-5
26-27	1.6055936313165257E-5	2.0872717207114834E-4	0.0	3.291466944198878E-4	6.422374525266103E-5
28-29	1.6055936313165257E-5	2.1675514022773096E-4	0.0	4.3351028045546193E-4	6.422374525266103E-5
30-31	1.6055936313165257E-5	2.4083904469747886E-4	0.0	8.509646245977586E-4	6.422374525266103E-5
32-33	1.6055936313165257E-5	2.4083904469747886E-4	0.0	0.0019829081346759096	6.422374525266103E-5
34-35	1.6055936313165257E-5	2.6492294916722676E-4	0.0	0.003500194116270026	8.027968156582629E-5
36-37	1.6055936313165257E-5	2.8900685363697466E-4	0.0	0.0058684447224619015	8.027968156582629E-5
38-39	1.6055936313165257E-5	2.8900685363697466E-4	0.0	0.009609477883429406	1.0436358603557418E-4
40-41	1.6055936313165257E-5	3.371746625764704E-4	0.0	0.01355923821646806	1.1239155419215681E-4
42-43	1.6055936313165257E-5	3.371746625764704E-4	0.0	0.01790236898917926	1.3647545866190468E-4
44-45	1.6055936313165257E-5	4.254823122988793E-4	0.0	0.022727177851285422	1.6055936313165257E-4
46-47	1.6055936313165257E-5	5.137899620212882E-4	0.0	0.0281460563569787	1.6055936313165257E-4
48-49	1.6055936313165257E-5	5.137899620212882E-4	0.0	0.03409478076100643	1.926712357579831E-4
50-51	1.6055936313165257E-5	5.298458983344535E-4	0.0	0.04004350516503415	2.0872717207114837E-4
52-53	1.6055936313165257E-5	5.298458983344535E-4	0.0	0.04574336255620782	2.3281107654089624E-4
54-55	1.6055936313165257E-5	5.298458983344535E-4	0.0	0.05137096823397225	2.4083904469747886E-4
56-57	1.6055936313165257E-5	5.298458983344535E-4	0.0	0.05654900769496804	2.488670128540615E-4
58-59	1.6055936313165257E-5	5.459018346476187E-4	0.0	0.06171901918780724	2.568949810106441E-4
60-61	1.6055936313165257E-5	5.459018346476187E-4	0.0	0.0670576180119347	2.649229491672267E-4
62-63	1.6055936313165257E-5	5.539298028042014E-4	0.0	0.07206707014164226	2.8900685363697466E-4
64-65	1.6055936313165257E-5	6.101255799002798E-4	0.0	0.07680357135402602	2.8900685363697466E-4
66-67	1.6055936313165257E-5	6.181535480568625E-4	0.0	0.08122698180830304	2.970348217935573E-4
68-69	1.6055936313165257E-5	6.26181516213445E-4	0.0	0.08523293791843778	3.130907581067225E-4
70-71	1.6055936313165257E-5	6.342094843700276E-4	0.0	0.08903819482465794	3.2111872626330515E-4
72-73	1.6055936313165257E-5	6.422374525266103E-4	0.0	0.09216910240572516	3.371746625764704E-4
74-75	1.6055936313165257E-5	6.582933888397756E-4	0.0	0.09494677938790275	3.371746625764704E-4
76-77	1.6055936313165257E-5	6.582933888397756E-4	0.0	0.09722672234437221	3.371746625764704E-4
78-79	1.6055936313165257E-5	6.582933888397756E-4	0.0	0.09860753286730442	3.371746625764704E-4
80-81	1.6055936313165257E-5	6.582933888397756E-4	0.0	0.09932202203324028	3.612585670462183E-4
82-83	1.6055936313165257E-5	6.582933888397756E-4	0.0	0.09974750434553917	3.853424715159662E-4
84-85	1.6055936313165257E-5	6.582933888397756E-4	0.0	0.10001242729470639	3.853424715159662E-4
86-87	1.6055936313165257E-5	6.904052614661061E-4	0.0	0.10011679088074196	3.853424715159662E-4
88	1.6055936313165257E-5	7.546290067187671E-4	0.0	0.1001408747852117	3.853424715159662E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7610	0.0	50.378963	1
GTATCAA	14025	0.0	37.429424	1
TCAACGC	16325	0.0	31.259907	4
ATCAACG	16365	0.0	31.15721	3
CAACGCA	16670	0.0	30.58076	5
TATCAAC	17335	0.0	29.71163	2
AACGCAG	17290	0.0	29.479908	6
ACGCAGA	19550	0.0	26.116001	7
CGCAGAG	19975	0.0	25.560345	8
GTACATG	16265	0.0	21.53409	1
GCAGAGT	23840	0.0	21.416437	9
TACATGG	16000	0.0	21.030178	2
ACATGGG	16180	0.0	20.184568	3
GTGGTAT	3790	0.0	20.095104	1
GAGTACT	14775	0.0	20.072872	12-13
CAGAGTA	23180	0.0	19.066507	10-11
AGAGTAC	21290	0.0	19.041819	10-11
CATGGGG	11945	0.0	17.939047	4
TGGTATC	3655	0.0	17.658754	2
GTACTTT	16945	0.0	17.400988	14-15
>>END_MODULE