FastQCFastQC Report
Fri 27 May 2016
SRR2138879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6472449
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72800.047.383411
GTATCAA132850.038.074051
TCAACGC148250.033.2462774
ATCAACG148450.033.0276573
CAACGCA151650.032.4725345
TATCAAC157550.031.4770642
AACGCAG157450.031.2756186
ACGCAGA181500.027.202477
CGCAGAG187650.026.2651048
GTGGTAT37750.024.9522951
GCAGAGT226200.021.6367869
TGGTATC40550.021.0018562
GTACATG173750.020.0265921
GAGTACT126800.019.85869612-13
TACATGG171250.019.6659322
ACATGGG174950.018.6095143
CAGAGTA221100.018.57623510-11
GCGTACA7700.017.8748571
AGAGTAC202050.017.52862410-11
GTACTTT144850.017.44378914-15
Produced by FastQC (version 0.11.5)