##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6472449
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.023663376876357	28.0	18.0	32.0	18.0	33.0
2	26.574143419283796	28.0	18.0	32.0	18.0	33.0
3	29.71868623453039	32.0	27.0	33.0	25.0	33.0
4	30.827349122411007	33.0	31.0	33.0	27.0	33.0
5	32.35249995789847	33.0	33.0	33.0	32.0	34.0
6	36.5572731434423	38.0	37.0	38.0	34.0	38.0
7	36.75905966968608	38.0	37.0	38.0	34.0	38.0
8	37.020184940816065	38.0	38.0	38.0	35.0	38.0
9	37.133003597247345	38.0	38.0	38.0	36.0	38.0
10-11	37.14830908671509	38.0	38.0	38.0	36.0	38.0
12-13	37.20069412675171	38.0	38.0	38.0	36.0	38.0
14-15	37.19247281824855	38.0	38.0	38.0	36.0	38.0
16-17	37.16792407325264	38.0	38.0	38.0	36.0	38.0
18-19	37.18849588463347	38.0	38.0	38.0	36.0	38.0
20-21	37.20688778020199	38.0	38.0	38.0	36.0	38.0
22-23	37.21528879298525	38.0	38.0	38.0	36.0	38.0
24-25	37.213825851714084	38.0	38.0	38.0	36.0	38.0
26-27	37.17674338641767	38.0	38.0	38.0	36.0	38.0
28-29	37.15068874350889	38.0	38.0	38.0	36.0	38.0
30-31	37.15594975336083	38.0	38.0	38.0	36.0	38.0
32-33	37.1494132359566	38.0	38.0	38.0	36.0	38.0
34-35	37.12585441921418	38.0	38.0	38.0	36.0	38.0
36-37	37.11012729882583	38.0	38.0	38.0	36.0	38.0
38-39	37.086500854877926	38.0	38.0	38.0	36.0	38.0
40-41	37.07926867762676	38.0	38.0	38.0	36.0	38.0
42-43	37.05206032443028	38.0	38.0	38.0	36.0	38.0
44-45	37.01229217978929	38.0	38.0	38.0	36.0	38.0
46-47	36.99332649830741	38.0	38.0	38.0	36.0	38.0
48-49	36.962933706407824	38.0	38.0	38.0	36.0	38.0
50-51	36.9283153964113	38.0	38.0	38.0	36.0	38.0
52-53	36.906271841540395	38.0	38.0	38.0	36.0	38.0
54-55	36.87466709897162	38.0	38.0	38.0	35.0	38.0
56-57	36.87678714707593	38.0	38.0	38.0	35.0	38.0
58-59	36.86261586972248	38.0	38.0	38.0	35.0	38.0
60-61	36.871885147069875	38.0	38.0	38.0	35.0	38.0
62-63	36.86033869088021	38.0	38.0	38.0	35.0	38.0
64-65	36.853561775221564	38.0	38.0	38.0	35.0	38.0
66-67	36.84220365719841	38.0	38.0	38.0	35.0	38.0
68-69	36.84346424980321	38.0	38.0	38.0	35.0	38.0
70-71	36.82424229087714	38.0	38.0	38.0	35.0	38.0
72-73	36.80710179465176	38.0	38.0	38.0	35.0	38.0
74-75	36.821924805541535	38.0	38.0	38.0	35.0	38.0
76-77	36.79887764957739	38.0	38.0	38.0	35.0	38.0
78-79	36.77519417141974	38.0	38.0	38.0	35.0	38.0
80-81	36.766711954317074	38.0	38.0	38.0	35.0	38.0
82-83	36.709649705828184	38.0	38.0	38.0	34.5	38.0
84-85	36.71087959156371	38.0	38.0	38.0	34.5	38.0
86-87	36.69904280815488	38.0	38.0	38.0	34.0	38.0
88-89	36.673595344360024	38.0	38.0	38.0	34.0	38.0
90-91	36.65122689719429	38.0	38.0	38.0	34.0	38.0
92-93	36.65114431191364	38.0	38.0	38.0	34.0	38.0
94-95	36.59579743001548	38.0	38.0	38.0	34.0	38.0
96-97	36.56188809501292	38.0	38.0	38.0	34.0	38.0
98-99	36.59090820946619	38.0	38.0	38.0	34.0	38.0
100	34.97327341428841	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	10.0
19	40.0
20	93.0
21	214.0
22	687.0
23	1915.0
24	4641.0
25	8896.0
26	15403.0
27	24661.0
28	36730.0
29	52199.0
30	70947.0
31	93002.0
32	121508.0
33	163730.0
34	241179.0
35	430896.0
36	1276742.0
37	3928951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.90342094622916	16.91407688187269	13.425505554389073	22.756996617509078
2	14.538967260335331	19.384815862079304	41.9173976988805	24.15881917870487
3	19.10015822449895	25.019695018068123	30.052256881436996	25.827889875995936
4	12.474675350860238	17.478948076686272	39.681100615856536	30.365275956596953
5	13.522154450002116	37.375945060931656	35.29175101284946	13.810149476216772
6	29.938320053445032	37.6972593351136	18.840008503742943	13.524412107698423
7	26.62485250945971	32.649140997480245	22.94154809099307	17.784458402066978
8	23.914178389045627	37.1640008287435	21.1586217210827	17.763199061128176
9	25.376453333197375	17.33399521572128	21.472506001978537	35.8170454491028
10-11	24.164330997432348	26.81113439441547	28.87358401742524	20.15095059072694
12-13	24.9104087185546	24.28020676563075	29.05095891833215	21.758425597482496
14-15	22.10786056406161	25.63687253464647	26.603067865038412	25.65219903625351
16-17	21.8052085076298	28.856758855882838	27.295626431355423	22.042406205131936
18-19	21.810716469144833	27.991514494745346	28.958667731487726	21.2391013046221
20-21	22.52842891731639	27.159390275990052	28.892619103571164	21.419561703122394
22-23	22.23507121819323	27.116087103471585	28.75014823040896	21.898693447926224
24-25	22.030743324598358	27.248743080928694	28.88913285041827	21.83138074405468
26-27	22.01571546112931	27.3574485875687	28.816906973676964	21.809928977625027
28-29	21.941989880287892	27.32193198888173	28.935238356101706	21.80083977472867
30-31	22.202715169300095	27.242367792487315	28.770781831438953	21.784135206773644
32-33	22.027756365015243	27.30582258112139	28.733799610176213	21.932621443687157
34-35	22.069687002296455	27.41214588133155	28.796739562587188	21.721427553784803
36-37	22.1176479943705	27.285726414412537	28.699673127683994	21.896952463532966
38-39	21.900509875192377	27.408740397371446	28.83276537284638	21.8579843545898
40-41	22.176194201588594	27.2425254668268	28.74809598790386	21.833184343680745
42-43	22.125602478451402	27.268768052950403	28.624133509906564	21.981495958691628
44-45	22.08134178855388	27.470263814981273	28.497865402422345	21.950528994042507
46-47	22.207767287893617	27.369683336370475	28.241227376787442	22.181321998948462
48-49	22.155189514005478	27.45249721797861	28.254348687474668	22.13796458054124
50-51	22.052322471027733	27.550557876067554	28.23450795432152	22.16261169858319
52-53	22.20901684593198	27.544582131014263	28.15711286121197	22.089288161841786
54-55	22.22980157246339	27.440333744761418	28.136999669327484	22.192865013447708
56-57	22.24453698395525	27.603633944985837	28.032477594274045	22.119351476784864
58-59	22.13183496741901	27.63352701750228	28.22206215142447	22.012575863654245
60-61	22.23169066824406	27.69405923734722	28.086414332580183	21.987835761828535
62-63	22.13953281568374	27.80145136838541	28.02368841154083	22.035327404390017
64-65	22.130000124093943	27.76431320207871	28.011510057813986	22.094176616013357
66-67	22.21678875595826	27.741824282338435	27.9407320281367	22.100654933566606
68-69	22.154343374794056	27.693113449381247	28.024246539529805	22.128296636294888
70-71	22.169709915046987	27.458742997977666	28.11194767630605	22.2595994106693
72-73	22.269270085118226	27.436460113786172	28.039285830258958	22.254983970836648
74-75	22.165869331614594	27.56544233575272	27.984741408176806	22.283946924455876
76-77	22.280020183517806	27.51925977503734	27.928355612245966	22.27236442919888
78-79	22.21239168981982	27.541553014216426	27.88242307544587	22.363632220517882
80-81	22.252889889623297	27.57677283189749	27.85467421725788	22.31566306122133
82-83	22.312023028812515	27.52941930049954	27.97090663634766	22.187651034340288
84-85	22.398402357905624	27.51727197244574	27.850643296274647	22.23368237337399
86-87	22.244154740204262	27.5712576063419	27.91779880904361	22.26678884441023
88-89	22.326241192128474	27.540098556858382	27.851471659269027	22.282188591744116
90-91	22.334411439602697	27.61305520526832	27.834621611944016	22.217911743184967
92-93	22.368585329216227	27.576932794216386	27.839078900413778	22.215402976153612
94-95	22.262442710481746	27.650790145939524	27.83616964126807	22.250597502310658
96-97	22.37839494315715	27.765891181947076	27.871250486143655	21.98446338875211
98-99	23.39332489341041	29.662636292403494	28.127012726791094	18.817026087395007
100	22.243467161824153	30.59035704048393	22.071041302056614	25.095134495635303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	880.0
1	841.5
2	1258.0
3	1892.5
4	2387.5
5	3282.0
6	4117.5
7	5217.0
8	7112.0
9	8242.5
10	7819.5
11	7494.0
12	8353.5
13	10143.5
14	13157.5
15	16520.0
16	19264.5
17	21553.5
18	22240.0
19	21080.5
20	19522.5
21	18756.5
22	19952.0
23	23689.5
24	29836.0
25	38782.0
26	50438.5
27	63533.5
28	77354.0
29	92244.5
30	107023.5
31	121594.0
32	136116.0
33	149453.0
34	163411.5
35	177144.5
36	191493.5
37	204995.0
38	215467.5
39	225693.5
40	236499.5
41	245650.5
42	253085.5
43	262159.0
44	268437.5
45	272104.0
46	274109.0
47	273913.0
48	273279.0
49	269892.0
50	263283.0
51	254341.5
52	243504.0
53	230394.0
54	216311.5
55	200439.0
56	183913.5
57	167007.5
58	148864.0
59	129525.0
60	108666.5
61	88608.0
62	70598.5
63	55382.5
64	42304.5
65	31880.0
66	24348.0
67	18248.5
68	13303.0
69	9886.5
70	7263.5
71	5074.0
72	3612.5
73	2484.5
74	1648.0
75	1073.0
76	637.5
77	364.5
78	238.0
79	162.5
80	114.5
81	70.5
82	48.0
83	32.5
84	20.0
85	12.0
86	7.0
87	6.0
88	3.5
89	2.0
90	2.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0032136213047024398
3	0.0
4	0.0
5	0.0017767617790422144
6	7.725051213227018E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.545072427973938E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	3.6493369925100195E-4
50-51	0.0017402175468014354
52-53	0.004170955195632195
54-55	0.0059237494964812926
56-57	0.006528958519238887
58-59	0.008365702035575611
60-61	0.007796436816284729
62-63	0.005719982978561863
64-65	0.0032745940106641467
66-67	0.0011212555280505103
68-69	5.351440033035053E-4
70-71	2.303036562565576E-4
72-73	4.920052721495853E-4
74-75	0.0
76-77	2.913001481170222E-4
78-79	1.9295534728424994E-4
80-81	8.348722812384162E-5
82-83	0.0
84-85	1.7010640722793464E-4
86-87	1.812251161223777E-4
88-89	3.8524340545154094E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	160.0
22-23	282.0
24-25	471.0
26-27	1394.0
28-29	3698.0
30-31	11264.0
32-33	14330.0
34-35	20027.0
36-37	31909.0
38-39	36348.0
40-41	35429.0
42-43	40989.0
44-45	48501.0
46-47	50247.0
48-49	46450.0
50-51	42884.0
52-53	41832.0
54-55	45506.0
56-57	47972.0
58-59	47182.0
60-61	46426.0
62-63	45203.0
64-65	48456.0
66-67	52530.0
68-69	53788.0
70-71	57586.0
72-73	48503.0
74-75	47988.0
76-77	50438.0
78-79	51752.0
80-81	51253.0
82-83	48749.0
84-85	48482.0
86-87	49916.0
88-89	52505.0
90-91	52972.0
92-93	52489.0
94-95	64174.0
96-97	201622.0
98-99	451298.0
100-101	4329397.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.27728604613894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15684566810599	77.78487962724783
2	7.013916122957825	12.102832952881162
3	1.655510406804584	4.284988347607168
4	0.569810796834454	1.966469164426582
5	0.24587369478101573	1.0606657547921376
6	0.1294363834372906	0.6700451927158105
7	0.06958788235543559	0.4202697541927487
8	0.043656516872435644	0.3013252635184977
9	0.02873551385711281	0.223129993385962
>10	0.08563133016358536	1.0613260918733083
>50	7.218910182252955E-4	0.0404308559576062
>100	1.9372821984999829E-4	0.030124926746994275
>500	5.337640797518727E-5	0.02845995960295821
>1k	2.6688184219671055E-5	0.025052115051222013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.180040970581615E-5	0.0
5	0.0	0.0	0.0	6.180040970581615E-5	0.0
6	1.0815071698517826E-4	0.0	0.0	6.180040970581615E-5	0.0
7	1.0815071698517826E-4	0.0	0.0	6.180040970581615E-5	0.0
8	1.0815071698517826E-4	1.5450102426454037E-5	0.0	7.725051213227018E-5	0.0
9	1.0815071698517826E-4	6.180040970581615E-5	0.0	1.236008194116323E-4	0.0
10-11	1.0815071698517826E-4	6.180040970581615E-5	0.0	2.472016388232646E-4	0.0
12-13	1.0815071698517826E-4	6.180040970581615E-5	0.0	3.012769973158537E-4	0.0
14-15	1.0815071698517826E-4	1.3132587062485932E-4	0.0	3.5535235580844284E-4	0.0
16-17	1.8540122911744843E-4	1.3905092183808633E-4	0.0	3.862525606613509E-4	0.0
18-19	2.0085133154390246E-4	1.4677597305131333E-4	0.0	4.017026630878049E-4	0.0
20-21	2.0085133154390246E-4	1.5450102426454036E-4	0.0	4.2487781672748603E-4	0.0
22-23	2.472016388232646E-4	2.0085133154390246E-4	0.0	4.3260286794071304E-4	0.0
24-25	2.7810184367617265E-4	2.085763827571295E-4	0.0	4.5577802158039405E-4	0.0
26-27	2.7810184367617265E-4	2.2402648518358352E-4	0.0	4.8667822643330217E-4	0.0
28-29	2.935519461026267E-4	2.3175153639681055E-4	0.0	6.257291482713884E-4	0.0
30-31	2.935519461026267E-4	2.472016388232646E-4	0.0	0.0011510326307708257	0.0
32-33	3.090020485290807E-4	2.472016388232646E-4	0.0	0.0026419675149236405	0.0
34-35	3.244521509555348E-4	2.9355194610262666E-4	0.0	0.004380104037899719	0.0
36-37	3.5535235580844284E-4	3.553523558084428E-4	0.0	0.007029796604036587	0.0
38-39	3.862525606613509E-4	3.862525606613509E-4	0.0	0.011000472927635275	0.0
40-41	3.862525606613509E-4	4.3260286794071304E-4	0.0	0.015519627887373079	0.0
42-43	4.017026630878049E-4	4.4032791915394004E-4	0.0	0.01966025533766276	0.0
44-45	4.017026630878049E-4	4.712281240068481E-4	0.0	0.023893583402511166	0.0
46-47	4.017026630878049E-4	4.944032776465292E-4	0.0	0.02943244512239494	0.0
48-49	4.017026630878049E-4	5.021283288597562E-4	0.0	0.03459277933283059	0.0
50-51	4.3260286794071304E-4	5.871038922052534E-4	0.0	0.03962178767264138	0.0
52-53	4.5577802158039405E-4	5.871038922052534E-4	0.0	0.04478212188307702	0.0
54-55	4.635030727936211E-4	5.871038922052534E-4	0.0	0.049950181144725894	0.0
56-57	4.635030727936211E-4	5.871038922052534E-4	0.0	0.054809238357845694	0.0
58-59	4.944032776465292E-4	5.871038922052534E-4	0.0	0.05942881898335545	0.0
60-61	5.175784312862102E-4	6.025539946317074E-4	0.0	0.06411020001857103	0.0
62-63	5.716537897787993E-4	6.180040970581614E-4	0.0	0.0690465077438231	0.0
64-65	5.716537897787993E-4	6.489043019110696E-4	0.0	0.07385921464966352	0.0
66-67	5.871038922052534E-4	6.489043019110696E-4	0.0	0.0784865203263865	0.0
68-69	6.025539946317074E-4	6.566293531242966E-4	0.0	0.08345372825649147	0.0
70-71	6.411792506978424E-4	6.720794555507506E-4	0.0	0.08818918465019965	0.0
72-73	6.952546091904316E-4	6.798045067639776E-4	0.0	0.0919512845910412	0.0
74-75	6.952546091904316E-4	6.798045067639776E-4	0.0	0.09450055149140611	0.0
76-77	7.338798652565667E-4	7.338798652565667E-4	0.0	0.09713479395511652	0.0
78-79	7.570550188962478E-4	7.493299676830207E-4	0.0	0.09883430522202646	0.0
80-81	7.570550188962478E-4	7.570550188962478E-4	0.0	0.09952183478000368	0.0
82-83	7.570550188962478E-4	7.570550188962478E-4	0.0	0.09996216269915761	0.0
84-85	7.570550188962478E-4	7.570550188962478E-4	0.0	0.10010893867220894	0.0
86-87	7.570550188962478E-4	7.570550188962478E-4	0.0	0.1002170893891941	0.0
88	7.570550188962478E-4	7.725051213227018E-4	0.0	0.10022481444040733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7280	0.0	47.38341	1
GTATCAA	13285	0.0	38.07405	1
TCAACGC	14825	0.0	33.246277	4
ATCAACG	14845	0.0	33.027657	3
CAACGCA	15165	0.0	32.472534	5
TATCAAC	15755	0.0	31.477064	2
AACGCAG	15745	0.0	31.275618	6
ACGCAGA	18150	0.0	27.20247	7
CGCAGAG	18765	0.0	26.265104	8
GTGGTAT	3775	0.0	24.952295	1
GCAGAGT	22620	0.0	21.636786	9
TGGTATC	4055	0.0	21.001856	2
GTACATG	17375	0.0	20.026592	1
GAGTACT	12680	0.0	19.858696	12-13
TACATGG	17125	0.0	19.665932	2
ACATGGG	17495	0.0	18.609514	3
CAGAGTA	22110	0.0	18.576235	10-11
GCGTACA	770	0.0	17.874857	1
AGAGTAC	20205	0.0	17.528624	10-11
GTACTTT	14485	0.0	17.443789	14-15
>>END_MODULE